"When dear old Mrs. Hay went back to town after staying with the Burnells she sent the children a doll's house." There are three girls in the Burnells , one is Isabel---the eldest sister, the second one ..."When dear old Mrs. Hay went back to town after staying with the Burnells she sent the children a doll's house." There are three girls in the Burnells , one is Isabel---the eldest sister, the second one is Lottie, and the third one is Kezia. Kazia is the heroine of the story. When the marvellous house stood in the garden, the Burnell children sounded as though they were in despair.展开更多
With the arrival of the ＂housing stock＂ in first - tier cities, the second - handhousing^market will become the dominant property market. This ardcle aim to the first - tiercities of second - hand housing prices and...With the arrival of the ＂housing stock＂ in first - tier cities, the second - handhousing^market will become the dominant property market. This ardcle aim to the first - tiercities of second - hand housing prices and new home price index for the empirical analysis, thedata related to the cointegration analysis found that the result of the first -tier cities real estatemarket in China, the new home price index is the significant factors influencing the second -hand house price indexi For Beijing, Shanghai second - hand housing and new home price in-dex time series johans test, found that there exists cointegration relationship between two varia-bles,the new city real estate market prices out of a line on the secondary market have clearguide. Therefore, the real estate market regulation aiming at the first -tier cities and the＂housing stock＂ should take the second - hand housing market as the main direction, startingwith the sale price and influencing factors of new houses. At the same time, in different cities,we should adhere to the city＇ s policies, reflect the policy differentiation, promote the reformof the real estate supply side, and promote the return of housing properties.展开更多
探讨仙鹤草-黄连(Agrimoniae Herba-Coptidis Rhizoma,XHC-HL)含药血清通过溶酶体相关膜蛋白2A型(lysosome-associated membrane protein type 2A,LAMP2A)介导的自噬对人结直肠癌HT29和HCT116细胞增殖、迁移、侵袭、凋亡的影响。通过生...探讨仙鹤草-黄连(Agrimoniae Herba-Coptidis Rhizoma,XHC-HL)含药血清通过溶酶体相关膜蛋白2A型(lysosome-associated membrane protein type 2A,LAMP2A)介导的自噬对人结直肠癌HT29和HCT116细胞增殖、迁移、侵袭、凋亡的影响。通过生物信息学分析LAMP2A在结直肠癌发生发展中的作用机制;蛋白免疫印迹(Westem blot,WB)检测LAMP2A蛋白在结直肠癌细胞株中的表达;利用慢病毒转染技术构建LAMP2A干扰HT29及过表达HCT116结直肠癌细胞模型;实时荧光定量聚合酶链式反应(real-time qPCR)实验检测转染效率;结直肠癌HT29和HCT116细胞分别给予不同浓度的仙鹤草-黄连含药血清,采用cell counting kit-8(CCK-8)法检测细胞增殖,筛选含药血清最佳干预浓度及时间。将HT29细胞分为空白对照(NC)组、XHC-HL(仙鹤草-黄连药对处理)组、XHC-HL+shLAMP2A(仙鹤草-黄连药对处理+干扰LAMP2A)组;将HCT116细胞分为空白对照(NC)组、XHC-HL(仙鹤草-黄连药对处理)组、XHC-HL+LAMP2A(仙鹤草-黄连药对处理+过表达LAMP2A)组,CCK-8检测细胞活力;平板克隆实验检测细胞增殖能力;划痕实验及Transwell迁移实验检测细胞迁移能力;Transwell侵袭实验检测细胞侵袭能力;流式细胞术检测细胞凋亡率;WB技术和real-time qPCR技术检测仙鹤草-黄连药对对结直肠癌细胞LAMP2A、热休克同源蛋白70(heat shock cognate protein 70,HSC70)、热休克蛋白90(heat shock protein 90,HSP90)、甘油醛-3-磷酸脱氢酶(glyceraldehyde-3-phosphate dehydrogenase,GAPDH)的蛋白表达水平及mRNA的影响。通过差异表达分析发现结直肠癌患者相对于正常对照样本的LAMP2A表达水平有显著升高,生存分析提示伴侣介导的自噬(chaperone-mediated autophagy,CMA)关键分子LAMP2A与结直肠癌的进展密切关联,通过基因集功能富集分析发现LAMP2A高表达患者显著上调了血管生成、免疫抑制等肿瘤进展相关信号通路,且免疫浸润分析发现LAMP2A高表达患者的肿瘤微环境会有更少的CD8 T细胞浸润。仙鹤草-黄连含药血清可以抑制HT29和HCT116细胞活力,最佳干预质量分数及时间为20%、48 h。与NC组相比,仙鹤草-黄连药对均可抑制HT29和HCT116细胞的增殖、迁移和侵袭,诱导细胞凋亡,药对处理干扰LAMP2A组均能进一步抑制肠癌细胞增殖、侵袭及迁移能力并促进凋亡,而过表达LAMP2A可逆转含药血清处理后结直肠癌细胞被抑制的增殖、迁移、侵袭能力,减少细胞凋亡率。仙鹤草-黄连药对含药血清对CMA具有抑制作用,通过LAMP2A介导的自噬,上调LAMP2A、HSC70、HSP90蛋白和基因的表达,下调底物蛋白GAPDH的表达发挥抗结直肠癌功能。综上,仙鹤草-黄连药对可通过下调CMA关键分子蛋白LAMP2A表达,抑制CMA活性,从而抑制结直肠癌细胞的增殖、迁移、侵袭,并诱导细胞凋亡。展开更多
文摘"When dear old Mrs. Hay went back to town after staying with the Burnells she sent the children a doll's house." There are three girls in the Burnells , one is Isabel---the eldest sister, the second one is Lottie, and the third one is Kezia. Kazia is the heroine of the story. When the marvellous house stood in the garden, the Burnell children sounded as though they were in despair.
文摘With the arrival of the ＂housing stock＂ in first - tier cities, the second - handhousing^market will become the dominant property market. This ardcle aim to the first - tiercities of second - hand housing prices and new home price index for the empirical analysis, thedata related to the cointegration analysis found that the result of the first -tier cities real estatemarket in China, the new home price index is the significant factors influencing the second -hand house price indexi For Beijing, Shanghai second - hand housing and new home price in-dex time series johans test, found that there exists cointegration relationship between two varia-bles,the new city real estate market prices out of a line on the secondary market have clearguide. Therefore, the real estate market regulation aiming at the first -tier cities and the＂housing stock＂ should take the second - hand housing market as the main direction, startingwith the sale price and influencing factors of new houses. At the same time, in different cities,we should adhere to the city＇ s policies, reflect the policy differentiation, promote the reformof the real estate supply side, and promote the return of housing properties.
