To compare the flesh quality of large yellow croaker cultured with three different modes, enclosure culture(EC), cage culture with feeding trash fish(CCF) and cage culture with feeding formula diet(CCD), thirty six fi...To compare the flesh quality of large yellow croaker cultured with three different modes, enclosure culture(EC), cage culture with feeding trash fish(CCF) and cage culture with feeding formula diet(CCD), thirty six fish individuals of EC(484.6 ± 79.8 g), CCF(432.7 ± 87.9 g) and CCD(416.9 ± 49.5 g) were sampled to measure body color, flesh water holding capacity, flesh proximate composition, amino acids and fatty acids composition, and collagen and inosinic acid contents. The analysis showed that there was no significant difference in the yellowness of skin and the contents of crude protein and inosinic acid between CCD and EC(P > 0.05), and the two modes showed higher values than CCF(P < 0.05). Most of the amino acids(AA) in flesh were higher in EC than in CCF(P < 0.05), but CCD showed no difference in AA composition from the other two modes(P > 0.05). EC and CCD had higher contents of collagen, free AA and lower drip loss than CC(P < 0.05). In polyunsaturated fatty acids, EC showed significantly higher levels of C20:5 n-3 and C22:6 n-3 than CCF and CCD(P < 0.05), but CCD had significantly higher C18:2 n-6 than CCF and EC(P < 0.05). In conclusion, the flesh quality of CCD fish is similar to EC fish, and these two modes have better flesh quality than CCF.展开更多
Stem cells (SCs), the undifferentiated biological cells, have the infinite capacity to self-renew and the pluripotent ability to differentiate. SCs and their derived products offer great promise for biomedical applica...Stem cells (SCs), the undifferentiated biological cells, have the infinite capacity to self-renew and the pluripotent ability to differentiate. SCs and their derived products offer great promise for biomedical applications such as cell therapy, tissue engineering, regenerative medicine and drug screening. However, the clinical applications of SCs require a large amount of SCs with high quality and the number of SCs from their tissue resources is very limited. Large-scale expansion is needed to generate homogeneous SCs with good biological characteristics for clinical application. This necessitates a bioreactor system to provide controllable and stable conditions for stem cell (SC) culture. Traditional methods of bioreactor for maintenance and expansion of cells rely on two-dimensional (2-D) culture techniques, leading to loss self-renewal ability and differentiation capacity upon long-term culture. New approaches for SC expansion with bioreactor employ three-dimensional (3-D) cell growth to mimic their environment in vivo. In this review, we summarize the application of bioreactors in SC culture.展开更多
基金supported by the Key Project of Science & Technology Promoting Agriculture (Shanghai municipal agricultural commission): Research of highlyefficient and ecologically formula feeds for large yellow croaker and groupers (No.2013-5-8)Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding (No.ZF1206)
文摘To compare the flesh quality of large yellow croaker cultured with three different modes, enclosure culture(EC), cage culture with feeding trash fish(CCF) and cage culture with feeding formula diet(CCD), thirty six fish individuals of EC(484.6 ± 79.8 g), CCF(432.7 ± 87.9 g) and CCD(416.9 ± 49.5 g) were sampled to measure body color, flesh water holding capacity, flesh proximate composition, amino acids and fatty acids composition, and collagen and inosinic acid contents. The analysis showed that there was no significant difference in the yellowness of skin and the contents of crude protein and inosinic acid between CCD and EC(P > 0.05), and the two modes showed higher values than CCF(P < 0.05). Most of the amino acids(AA) in flesh were higher in EC than in CCF(P < 0.05), but CCD showed no difference in AA composition from the other two modes(P > 0.05). EC and CCD had higher contents of collagen, free AA and lower drip loss than CC(P < 0.05). In polyunsaturated fatty acids, EC showed significantly higher levels of C20:5 n-3 and C22:6 n-3 than CCF and CCD(P < 0.05), but CCD had significantly higher C18:2 n-6 than CCF and EC(P < 0.05). In conclusion, the flesh quality of CCD fish is similar to EC fish, and these two modes have better flesh quality than CCF.
文摘Stem cells (SCs), the undifferentiated biological cells, have the infinite capacity to self-renew and the pluripotent ability to differentiate. SCs and their derived products offer great promise for biomedical applications such as cell therapy, tissue engineering, regenerative medicine and drug screening. However, the clinical applications of SCs require a large amount of SCs with high quality and the number of SCs from their tissue resources is very limited. Large-scale expansion is needed to generate homogeneous SCs with good biological characteristics for clinical application. This necessitates a bioreactor system to provide controllable and stable conditions for stem cell (SC) culture. Traditional methods of bioreactor for maintenance and expansion of cells rely on two-dimensional (2-D) culture techniques, leading to loss self-renewal ability and differentiation capacity upon long-term culture. New approaches for SC expansion with bioreactor employ three-dimensional (3-D) cell growth to mimic their environment in vivo. In this review, we summarize the application of bioreactors in SC culture.
