Odorant binding proteins （OBPs） in insects are postulated to solubilize and transport the hydrophobic odorants across the hydrophilic antennal lymph to the olfactory receptors （ORs） located on the dendrite membran...Odorant binding proteins （OBPs） in insects are postulated to solubilize and transport the hydrophobic odorants across the hydrophilic antennal lymph to the olfactory receptors （ORs） located on the dendrite membrane of the sensory neurons. OBPs in adult insects have been intensively reported, but those in larvae are rarely addressed. In our study, a full-length OBP cDNA, namely SexiOBP13, was cloned by RT-PCR and RACE strategy from the heads of Spodoptera exigua larvae. The quantitative real-time PCR （qPCR） measurement indicated that SexiOBP13 was highly expressed in larval head, but very low in other parts of larva and was not detected in any tissues of adult. The binding affinities of SexiOBP13 to plant volatiles and female sex pheromone components were measured by competitive binding assays. Interestingly, SexiOBP13 displayed a high binding affinity （Ki=3.82 IJmol L-1） to Z9,E12-14：Ac, the major sex pheromone component of S. exigua, while low affinities to the tested host plant volatiles （Ki〉27 μmol L-l）. The behavioral tests further confirmed that Z9,E12-14：Ac was indeed active to elicit the behavioral activity of the third instar larvae of S. exigua. Taken together, our results suggest that SexiOBP13 may play a role in reception of female sex pheromone in S. exigua larvae. The ecological significance of the larvae preference to the adult female sex pheromone was discussed.展开更多
研究雷氏黄萤Luciola leii Fu and Ballantyne幼虫的呼吸系统及其呼吸行为。结果表明：雷氏黄萤幼虫的呼吸系统中只有气管无气囊。前胸、中胸和后胸均分布有气门,无气管鳃,腹部1-8节分布有气门和气管鳃,气门腔基部和气管鳃基部相连,呈...研究雷氏黄萤Luciola leii Fu and Ballantyne幼虫的呼吸系统及其呼吸行为。结果表明：雷氏黄萤幼虫的呼吸系统中只有气管无气囊。前胸、中胸和后胸均分布有气门,无气管鳃,腹部1-8节分布有气门和气管鳃,气门腔基部和气管鳃基部相连,呈“√”状,气管鳃内气管与气门气管相连通。雷氏黄萤幼虫的呼吸行为分为3种：利用胸部气门呼吸、腹部气门呼吸和气管鳃呼吸,其中以腹部气门呼吸为主。展开更多
The effect of different bean varieties on the performance of the bruchid beetle Zabrotes subfasciatus Boheman （Coleoptera： Bruchidae）, was determined by using wild and cultivated seeds of the genus Phaseolus. Resul...The effect of different bean varieties on the performance of the bruchid beetle Zabrotes subfasciatus Boheman （Coleoptera： Bruchidae）, was determined by using wild and cultivated seeds of the genus Phaseolus. Results showed that the quality of the host plant affected the performance and the oviposition behavior of female beetles. Overall, bruchid performance was higher on cultivated seeds than on wild seeds. It was also found that the oviposition behavior and the performance of their offspring differed between females that originated from wild versus cultivated seeds. We also demonstrated the importance of a genetic component in bruchid performance： longevity, fecundity, larval development, adult size, and the sex ratio of the progeny varied according to their matemal lineage （‘family＇ ）. For example, on the same host type, some females laid twice as many eggs as females from other‘families＇, and the sex ratio among the offspring correlated with that of their mother. Thus, the performance and behavior of Z. subfasciatus are not only affected by environmental factors such as the quality of the seeds on which they develop, but also have a genetic basis which can counterbalance a less suitable quality of the host plant. For a crop pest such as Z. subfasciatus, its ability to survive and adapt on host plants of differing quality may be an important attribute to consider for pest management.展开更多
基金supported by a grant from the National Natural Science Foundation of China (31372264)the Special Fund for Agro-Scientific Research in the Public Interest,China (201203036)
文摘Odorant binding proteins （OBPs） in insects are postulated to solubilize and transport the hydrophobic odorants across the hydrophilic antennal lymph to the olfactory receptors （ORs） located on the dendrite membrane of the sensory neurons. OBPs in adult insects have been intensively reported, but those in larvae are rarely addressed. In our study, a full-length OBP cDNA, namely SexiOBP13, was cloned by RT-PCR and RACE strategy from the heads of Spodoptera exigua larvae. The quantitative real-time PCR （qPCR） measurement indicated that SexiOBP13 was highly expressed in larval head, but very low in other parts of larva and was not detected in any tissues of adult. The binding affinities of SexiOBP13 to plant volatiles and female sex pheromone components were measured by competitive binding assays. Interestingly, SexiOBP13 displayed a high binding affinity （Ki=3.82 IJmol L-1） to Z9,E12-14：Ac, the major sex pheromone component of S. exigua, while low affinities to the tested host plant volatiles （Ki〉27 μmol L-l）. The behavioral tests further confirmed that Z9,E12-14：Ac was indeed active to elicit the behavioral activity of the third instar larvae of S. exigua. Taken together, our results suggest that SexiOBP13 may play a role in reception of female sex pheromone in S. exigua larvae. The ecological significance of the larvae preference to the adult female sex pheromone was discussed.
文摘研究雷氏黄萤Luciola leii Fu and Ballantyne幼虫的呼吸系统及其呼吸行为。结果表明：雷氏黄萤幼虫的呼吸系统中只有气管无气囊。前胸、中胸和后胸均分布有气门,无气管鳃,腹部1-8节分布有气门和气管鳃,气门腔基部和气管鳃基部相连,呈“√”状,气管鳃内气管与气门气管相连通。雷氏黄萤幼虫的呼吸行为分为3种：利用胸部气门呼吸、腹部气门呼吸和气管鳃呼吸,其中以腹部气门呼吸为主。
文摘采用乙醇和二氯甲烷分别浸提核桃叶片,获得粗提物,并在室内测定了不同浓度粗提物溶液对马铃薯块茎蛾(Phthorimaea operculella)成虫的产卵选择性及幼虫钻蛀性的影响。结果表明:两种核桃叶粗提物均对马铃薯块茎蛾的产卵以及幼虫钻蛀表现出强烈的抑制效果,而且这种抑制效果随着粗提物浓度的上升而增强;2~162 g E/L的两种粗提物溶液均不影响马铃薯块茎蛾雌蛾的交配行为;两种粗提物对块茎蛾幼虫钻蛀行为的药效能够持续36 h。
基金Acknowledgments We would like to acknowledge the Instituto de Ecologia, UNAM, Mexico for providing laboratory space, the use of equipment and transportation to the field sites. In particular we thank Alicia Callejas and Leonor Ceballos for their help in the field and the laboratory, and Constantino Macias for his support during our work in Mexico. We are grateful to Jacqueline Moret for statistical advice and to John Woodley for reviewing the manuscript. Financial support was provided by the Swiss National Science Foundation, project No. 3100.064821.01/1.
文摘The effect of different bean varieties on the performance of the bruchid beetle Zabrotes subfasciatus Boheman （Coleoptera： Bruchidae）, was determined by using wild and cultivated seeds of the genus Phaseolus. Results showed that the quality of the host plant affected the performance and the oviposition behavior of female beetles. Overall, bruchid performance was higher on cultivated seeds than on wild seeds. It was also found that the oviposition behavior and the performance of their offspring differed between females that originated from wild versus cultivated seeds. We also demonstrated the importance of a genetic component in bruchid performance： longevity, fecundity, larval development, adult size, and the sex ratio of the progeny varied according to their matemal lineage （‘family＇ ）. For example, on the same host type, some females laid twice as many eggs as females from other‘families＇, and the sex ratio among the offspring correlated with that of their mother. Thus, the performance and behavior of Z. subfasciatus are not only affected by environmental factors such as the quality of the seeds on which they develop, but also have a genetic basis which can counterbalance a less suitable quality of the host plant. For a crop pest such as Z. subfasciatus, its ability to survive and adapt on host plants of differing quality may be an important attribute to consider for pest management.
