Establishing intraspecific breeding and hybridization programs and determining genetic variability are two important issues for aquaculture. However, interspecific hybridization to improve growth and feeding efficienc...Establishing intraspecific breeding and hybridization programs and determining genetic variability are two important issues for aquaculture. However, interspecific hybridization to improve growth and feeding efficiency is limited. For this purpose, the embryonic and larval development of reciprocal crosses of Clarias gariepinus (Burchell, 1822) and Clarias jaensis (Boulenger, 1909) were studied under laboratory conditions. The fertilization rate varied from 63.33% to 92%, while the hatching rate ranged from 55.68% to 76% with the highest value in hybrids ♀Cg × ♂Cj. Crosses between ♀Cj × ♂Cj, ♀Cg × ♂Cj and ♀Cj × ♂Cg had embryonic stages similar to those of the pure sib ♀Cg x ♂Cg. All crosses, however, had different timing for the various embryological stages. Hatching occurred at 32 h 15 min and 38 h for ♀Cj × ♂Cj and ♀Cj × ♂Cg, and 23 h and 23 h 30 min, respectively, for ♀Cg × ♂Cg and ♀Cg × ♂Cj. However, both crosses produced viable larvae until the first external feeding. The external morphological features of the larvae were completely formed by the 10th day after hatching. The body forms of the crosses at this time were indistinguishable from the pure sib. This study thus laid the groundwork for further comparative studies on hybrid performance and characterization.展开更多
The effectiveness of current control measures against Aedes mosquitoes remains low, resulting in persistent epidemics in urban areas. The emergence of resistant mosquito populations to chemical insecticides highlights...The effectiveness of current control measures against Aedes mosquitoes remains low, resulting in persistent epidemics in urban areas. The emergence of resistant mosquito populations to chemical insecticides highlights the need for novel, environmentally friendly, cost-effective control strategies. This study explored the potential of environmental bacterial isolates to biocontrol wild Aedes larvae. Initially, we collected bacterial samples from infectious masses of Aedes fluviatilis larvae. The isolated bacteria were identified using biochemical, enzymatic, and molecular methods, including 16S rRNA sequencing and MALDI-TOF. Previously, Aeromonas hydrophila and Bacillus thuringiensis isolated from these infectious masses showed limited Aedes larval inhibition. Consequently, we screened additional environmental isolates from the bacteriotheque. Six isolates previously identified were tested: Chromobacterium violaceum, Enterobacter cloacae, Bacillus cereus, Bacillus sphaericus, and two strains of Bacillus thuringiensis israelensis. Among these strains, B. thuringiensis and C. violaceum exhibited significant inhibitory activities against wild Aedes larvae. Bacillus thuringiensis cultures grown under daylight conditions showed a slight ability to inhibit Aedes larvae. The potential of B. thuringiensis and C. violaceum strains studied, along with optimized culture growth conditions, will be further investigated to develop bioinsecticide products to provide safer and more sustainable alternatives for controlling larvae of Aedes mosquitoes.展开更多
The last-instar larval external morphologies of Illiberis tenuis (Butler), Chalcosia remota (Walker) and Pryeria sinica Moore of Zygaenidae are described and illustrated. All the specimens are deposited in the Insect ...The last-instar larval external morphologies of Illiberis tenuis (Butler), Chalcosia remota (Walker) and Pryeria sinica Moore of Zygaenidae are described and illustrated. All the specimens are deposited in the Insect Collection of Department of Forest Resources Protection, Kangwon National University, Korea.展开更多
The last-instar larval external morphologies of Orthaga achatina Butler, Macalla sp. and Locastra muscosalis （Walker） of Epipaschiinae are described and illustrated. All specimens examined are deposited in the Colle...The last-instar larval external morphologies of Orthaga achatina Butler, Macalla sp. and Locastra muscosalis （Walker） of Epipaschiinae are described and illustrated. All specimens examined are deposited in the Collection of College of Life Sciences, China Jiliang University, China. Biological information such as living habit also is included.展开更多
The last-stage larval external morphologies of Pleuroptya rulalis （Scopoli）, Pleuroptya harutai （Inoue） and Botyodes diniasalis （Walker） of Pyraustinae are described and illustrated. All specimens are deposited ...The last-stage larval external morphologies of Pleuroptya rulalis （Scopoli）, Pleuroptya harutai （Inoue） and Botyodes diniasalis （Walker） of Pyraustinae are described and illustrated. All specimens are deposited in the Insect Collection of Department of Forest Resources Protection, Kangwon National University, Korea.展开更多
文摘Establishing intraspecific breeding and hybridization programs and determining genetic variability are two important issues for aquaculture. However, interspecific hybridization to improve growth and feeding efficiency is limited. For this purpose, the embryonic and larval development of reciprocal crosses of Clarias gariepinus (Burchell, 1822) and Clarias jaensis (Boulenger, 1909) were studied under laboratory conditions. The fertilization rate varied from 63.33% to 92%, while the hatching rate ranged from 55.68% to 76% with the highest value in hybrids ♀Cg × ♂Cj. Crosses between ♀Cj × ♂Cj, ♀Cg × ♂Cj and ♀Cj × ♂Cg had embryonic stages similar to those of the pure sib ♀Cg x ♂Cg. All crosses, however, had different timing for the various embryological stages. Hatching occurred at 32 h 15 min and 38 h for ♀Cj × ♂Cj and ♀Cj × ♂Cg, and 23 h and 23 h 30 min, respectively, for ♀Cg × ♂Cg and ♀Cg × ♂Cj. However, both crosses produced viable larvae until the first external feeding. The external morphological features of the larvae were completely formed by the 10th day after hatching. The body forms of the crosses at this time were indistinguishable from the pure sib. This study thus laid the groundwork for further comparative studies on hybrid performance and characterization.
文摘The effectiveness of current control measures against Aedes mosquitoes remains low, resulting in persistent epidemics in urban areas. The emergence of resistant mosquito populations to chemical insecticides highlights the need for novel, environmentally friendly, cost-effective control strategies. This study explored the potential of environmental bacterial isolates to biocontrol wild Aedes larvae. Initially, we collected bacterial samples from infectious masses of Aedes fluviatilis larvae. The isolated bacteria were identified using biochemical, enzymatic, and molecular methods, including 16S rRNA sequencing and MALDI-TOF. Previously, Aeromonas hydrophila and Bacillus thuringiensis isolated from these infectious masses showed limited Aedes larval inhibition. Consequently, we screened additional environmental isolates from the bacteriotheque. Six isolates previously identified were tested: Chromobacterium violaceum, Enterobacter cloacae, Bacillus cereus, Bacillus sphaericus, and two strains of Bacillus thuringiensis israelensis. Among these strains, B. thuringiensis and C. violaceum exhibited significant inhibitory activities against wild Aedes larvae. Bacillus thuringiensis cultures grown under daylight conditions showed a slight ability to inhibit Aedes larvae. The potential of B. thuringiensis and C. violaceum strains studied, along with optimized culture growth conditions, will be further investigated to develop bioinsecticide products to provide safer and more sustainable alternatives for controlling larvae of Aedes mosquitoes.
文摘The last-instar larval external morphologies of Illiberis tenuis (Butler), Chalcosia remota (Walker) and Pryeria sinica Moore of Zygaenidae are described and illustrated. All the specimens are deposited in the Insect Collection of Department of Forest Resources Protection, Kangwon National University, Korea.
基金The project was supported by the Zhejiang Natural Science Foundation(Y304454)
文摘The last-instar larval external morphologies of Orthaga achatina Butler, Macalla sp. and Locastra muscosalis （Walker） of Epipaschiinae are described and illustrated. All specimens examined are deposited in the Collection of College of Life Sciences, China Jiliang University, China. Biological information such as living habit also is included.
文摘The last-stage larval external morphologies of Pleuroptya rulalis （Scopoli）, Pleuroptya harutai （Inoue） and Botyodes diniasalis （Walker） of Pyraustinae are described and illustrated. All specimens are deposited in the Insect Collection of Department of Forest Resources Protection, Kangwon National University, Korea.
