Proteomics is becoming more and more mature, but the detection of low abundance proteins is still a difficult task. Laser-induced fluorescence（LIF） detection is one of the most sensitive detection methods in a capil...Proteomics is becoming more and more mature, but the detection of low abundance proteins is still a difficult task. Laser-induced fluorescence（LIF） detection is one of the most sensitive detection methods in a capillary electrophoresis（CE） system. However, most proteins do not exhibit favourable native fluorescence, a derivatization procedure is necessary for LIF detection of proteins. Since the derivatization reaction between protein and fluorescent reagent takes place after the separation of protein, the separation cannot be compromised by multiple derivatization products, the post-column derivatization becomes an attractive method for derivatization in CE-LIF system.展开更多
基金Supported by the Surface Project of the National Natural Science Foundation of China（No.21075039/B050106） and the Spe- cialized Research Fund for the Doctoral Program of Higher Education of China（No.20100074110016）.
文摘Proteomics is becoming more and more mature, but the detection of low abundance proteins is still a difficult task. Laser-induced fluorescence（LIF） detection is one of the most sensitive detection methods in a capillary electrophoresis（CE） system. However, most proteins do not exhibit favourable native fluorescence, a derivatization procedure is necessary for LIF detection of proteins. Since the derivatization reaction between protein and fluorescent reagent takes place after the separation of protein, the separation cannot be compromised by multiple derivatization products, the post-column derivatization becomes an attractive method for derivatization in CE-LIF system.
