[Objective]The paper was to investigate the mechanism of Bacillus coagulans preparation in improving production performance of laying hens in late period of laying.[Method]A total of 648 individuals of"Jingfen 1&...[Objective]The paper was to investigate the mechanism of Bacillus coagulans preparation in improving production performance of laying hens in late period of laying.[Method]A total of 648 individuals of"Jingfen 1"laying hens(66 weeks of age)in late period of laying were randomly divided into four groups,six replicates each group,and each group had 27 individuals of laying hens.The laying hens in control group were fed with basal diet,and those in experimental groupsⅠ,Ⅱ and Ⅲ were fed with the basal diets added with 3.33×10^6,1×10^7,3.33×10^7 CFU/g B.coagulans,respectively.The pretrial lasted one week,and the formal test lasted eight weeks.[Result]Compared with the control group,the laying rate in group Ⅰ increased significantly,and the feed-gain ratio in experimental group Ⅱ decreased noticeably while the spleen index increased remarkably;the alkaline phosphatase activities and blood calcium content in experimental groups Ⅰ,Ⅱ and Ⅲ increased significantly(P<0.05),while the triglyceride content decreased remarkably(P<0.05);the urea content in experimental groups Ⅰ and Ⅱ decreased obviously(P<0.05).Adding B.coagulans significantly increased the specific activity of amylase,lipase and protease in various intestinal mucosa of small intestine(P<0.05);adding B.coagulans significantly increased the content of follicle-stimulating hormone(FSH)and estradiol(E2),and significantly increased the mRNA expression of follicle stimulating hormone receptor gene(FSHR).[Conclusion]B.coagulans preparation could significantly improve the production performance of laying hens in late period of laying;appropriately enhance the immune capacity of laying hens;improve the serum biochemical indicators;increase the activity of digestive enzymes in the small intestine;and promote the release of gonadal hormone and the mRNA expression of FSHR gene in the ovary.展开更多
The widely-developed,mixed clastic-carbonate succession in the northern Qaidam Basin records the paleo-environment changes under the glacial activity during the Late Paleozoic icehouse period in the context of regiona...The widely-developed,mixed clastic-carbonate succession in the northern Qaidam Basin records the paleo-environment changes under the glacial activity during the Late Paleozoic icehouse period in the context of regional tectonic stability,however,the depositional environment and sequence stratigraphy characteristics of the mixed deposits is rarely reported and still not clear.Combined the latest drilling wells data,we analyzed the sedimentary and stratigraphic characterization of the mixed strata via detailed field outcrops and core observations and thin section microscopic observations and recognized three depositional systems,including progradational coastal system,incised valley system,and carbonate-dominated marine shelf system,and identified four third-order sequences,SQ1,SQ2,SQ3 and SQ4,consisting of LST,TST,and HST.The depositional environment is overall belonged to marine-continental transition context and shifted from marine to continental environment frequently,showing an evolutionary pattern from marine towards terrestrial-marine transition and then back into the marine environment again in the long-term,which was controlled by the regional tectonic subsidence and the high-frequency and large-amplitude sea-level changes due to the Late Paleozoic glacial activity.The result is of significance in understanding the evolution of the Qinghai-Tibet Plateau and the sedimentation-climate response.展开更多
The relationship between the evolution of human societies and their living environments has been discussed intensively in recent decades.It is important to understand the patterns and mechanisms of human–environment ...The relationship between the evolution of human societies and their living environments has been discussed intensively in recent decades.It is important to understand the patterns and mechanisms of human–environment interaction and evolution in order to cope with rapidly changing environments in the modern world.We reviewed the results of dating,archaeobotanical,and zooarchaeological studies from 139 prehistoric sites in the Northeast Tibetan Plateau(NETP)and contiguous regions(NETP-CR:i.e.,the Hexi Corridor and western margin of the Chinese Loess Plateau)and compared them with paleoenvironmental records to study variations in spatiotemporal patterns of social development in the area and their relationships with climate and vegetation changes.Our results show that hunter-gatherer groups occupied vast areas of the NETP at low intensities during^15,000–5500 BP(years before present).This was directly related to forest cover and climate change.An increase in temperature and precipitation turnover of vegetation from desert steppe to alpine meadow/subalpine shrub,and recovery of animal population on the Tibetan Plateau created more food resources and space for hunter-gatherers.Millet farmers settled extensively below 2500 m a.s.l.(meters above sea level)in the NETP-CR between^5500 and 3600 BP,and might have coexisted with hunting groups above 2500 m a.s.l.via subsistence exchange.The distribution of human settlements during that period was affected by climate change,with the relatively warm and wet climate promoting the expansion of millet agriculture to the NETP-CR during 5500–4500 BP,while climate deterioration caused humans to retreat to lower altitudes.During 3600–2200 BP,a range of livelihoods emerged in different regions of the NETPCR.This was promoted by early trans-Eurasian exchange and the development of an agro-pastoral economy that utilized cold-tolerant crops and livestock.This eventually promoted the expansion of permanent human settlements to high-altitude areas in the NETP.This study found that human societies adopted various strategies to adapt to the changing living environment throughout late prehistoric times in the NETP-CR.The results provide a long-term perspective on the trajectory of regional socio-environmental co-evolution.展开更多
During the late Qing Dynasty and the early period of the Republic of China(1870-1930),the social classes in Yangzhou were basically dominated by officials,businessmen,and scholars.In the building number,scale,and func...During the late Qing Dynasty and the early period of the Republic of China(1870-1930),the social classes in Yangzhou were basically dominated by officials,businessmen,and scholars.In the building number,scale,and functional evolution of their home gardens,these three classes were different,reflecting abundant connotation of characteristic of the time and regional culture,revealing the inheritance path of Yangzhou culture,as well as the richness and diversity of home gardens in Yangzhou during the late Qing Dynasty and the early period of the Republic of China.展开更多
As a world-level author,Shakespeare is more and more popular to Chinese readers.However,Shakespeare is hard to un⁃derstand for students who are not familiar to British history.To facilitate the understanding of Shakes...As a world-level author,Shakespeare is more and more popular to Chinese readers.However,Shakespeare is hard to un⁃derstand for students who are not familiar to British history.To facilitate the understanding of Shakespeare’s plays,this paper intro⁃duces the historical background and historical events depicted in the works of Shakespeare and lists several themes discussed in Shakespeare’s history plays and tragedies.展开更多
Objective To investigate the therapeutic efficacy of castration with 125 I brachtherapy in middle and late stage prostate cancer. Methods Sixty-six patients with prostate cancer from 2004 to 2009 were analyzed,40 were...Objective To investigate the therapeutic efficacy of castration with 125 I brachtherapy in middle and late stage prostate cancer. Methods Sixty-six patients with prostate cancer from 2004 to 2009 were analyzed,40 were at clinical stage C and 26 were at clinical stage D,展开更多
The Yangbishan iron-tungsten deposit in the Shuangyashan area of Heilongjiang Province is located in the center of the Jiamusi Massif in northeastern China. The rare earth element and trace element compositions of the...The Yangbishan iron-tungsten deposit in the Shuangyashan area of Heilongjiang Province is located in the center of the Jiamusi Massif in northeastern China. The rare earth element and trace element compositions of the scheelite show that it formed in a reducing environment and inherited the rare earth element features of the ore-forming fluid. The geochemical characteristics of the gneissic granite associated with the tungsten mineralization show that the magma formed in this reducing environment and originated from the partial melting of metamorphosed shale that contained organic carbon and was enriched with tungsten. In addition, in situ Hf isotopic analysis of zircons from the gneissic granite indicates that they probably originated from the partial melting of a predominantly Paleo-Mesoproterozoic crustal source. According to LA-ICP-MS zircon dating, the Yangbishan ore- related gneissic granite has an Early Paleozoic crystallization age of 520.6 + 2.8 Ma. This study, together with previous data, indicates that the massifs of northeastern China, including Erguna, Xing'an, Songliao, Jiamusi, and Khanka massifs, belonged to an orogenic belt that existed along the southern margin of the Siberian Craton during the late Pan-African period. The significant continental movements of this orogeny resulted in widespread magmatic activity in northeastern China from 530 Ma to 470 Ma under a tectonic setting that transitioned from compressional syn-collision to extensional post- collision.展开更多
The Bohai Bay Basin is a typical oil-prone basin, in which natural gas geological reserves have a small proportion. In this basin, the gas source rock is largely medium-deep lake mudstone with oil-prone type Ⅱ2-Ⅱ1 k...The Bohai Bay Basin is a typical oil-prone basin, in which natural gas geological reserves have a small proportion. In this basin, the gas source rock is largely medium-deep lake mudstone with oil-prone type Ⅱ2-Ⅱ1 kerogens, and natural gas preservation conditions are poor due to active late tectonic movements. The formation conditions of large natural gas fields in the Bohai Bay Basin have been elusive. Based on the exploration results of Bohai Bay Basin and comparison with large gas fields in China and abroad, the formation conditions of conventional large-scale natural gas reservoirs in the Bohai Bay Basin were examined from accumulation dynamics, structure and sedimentation. The results show that the formation conditions of conventional large natural gas reservoirs in Bohai Bay Basin mainly include one core element and two key elements. The core factor is the strong sealing of Paleogene "quilt-like" overpressure mudstone. The two key factors include the rapid maturation and high-intensity gas generation of source rock in the late stage and large scale reservoir. On this basis, large-scale nature gas accumulation models in the Bohai Bay Basin have been worked out, including regional overpressure mudstone enriching model, local overpressure mudstone depleting model, sand-rich sedimentary subsag depleting model and late strongly-developed fault depleting model. It is found that Bozhong sag, northern Liaozhong sag and Banqiao sag have favorable conditions for the formation of large-scale natural gas reservoirs, and are worth exploring. The study results have important guidance for exploration of large scale natural gas reservoirs in the Bohai Bay Basin.展开更多
The blood neutrophils to lymphocytes ratio (NLR) reflects the physiological homeostasis between lymphopoiesis and myelopoiesis, and its elevation serves as a harmful sign in many pathologies, partially, late rejection...The blood neutrophils to lymphocytes ratio (NLR) reflects the physiological homeostasis between lymphopoiesis and myelopoiesis, and its elevation serves as a harmful sign in many pathologies, partially, late rejection of allograft. The stem and young lymphoid cells have regenerative-trophic properties, which can affect the relevance of NLR, being opposed to immune properties, associated with bulk lymphocytes. In the present article, we have analyzed for the first time the applicability of NLR’s analogs with stem and immature blood cells for monitoring harmful long-term shifting from lymphopoiesis to myelopoiesis in transplant’s recipients received conventional immunosuppressive treatment. In opposition to conventional NLR, the ratio of subpopulation of CD31 cells committed to the liver tissue by alfa-fetoprotein (AFP), seems sensitive enough for such monitoring several years after transplantation of the liver from the dead.展开更多
Different arguments on the relationship between railway construction and economic development exist in literature. As the railway construction of Henan in the period of late Qing Dynasty(1644-1911) and early Republic ...Different arguments on the relationship between railway construction and economic development exist in literature. As the railway construction of Henan in the period of late Qing Dynasty(1644-1911) and early Republic of China(1912-1949) provides a nearly natural experiment to throw new light on this topic, this paper creates county-level panel data for this period and conducts a further test with a difference-in-difference strategy. While the effect of railway is demonstrated to be positive in some literature yet negative in others, this paper identifies some major intergroup differences: railway lines put into service after the 1910 s and connecting central cities promoted population growth in regions along the railway lines, enhanced their capacity to cope with external market shocks, yet they did not improve integration with the intra-provincial market. By contrast, the railway lines put into operation in the 1930 s and connecting hinterland areas not only improved regional market integration but contributed to higher living standards as well. The implication is that the effects of railway are subject to the economic relations of various localities along the railway lines and the economic attributes of various sectors. thus railways cannot be generally defined to be positive or negative. This conclusion helps explain the disagreements in empirical studies and highlight the impact of railways on the regional economic structure.展开更多
Human activities are usually considered to have had impacts on forest degradation and/or changes of forest composition in the past.The Tibetan Plateau,an environmentally sensitive area,has witnessed a high intensity o...Human activities are usually considered to have had impacts on forest degradation and/or changes of forest composition in the past.The Tibetan Plateau,an environmentally sensitive area,has witnessed a high intensity of human activities since the late Neolithic,but the evidence is still incomplete concerning how human activities affected the environment,especially vegetation changes.This may result,in part,from the fact that research on prehistoric wood utilization by humans in the northeast Tibetan Plateau(NETP)is scarce,especially from the perspective of charcoal analysis,which is an archaeological index directly related to human activities.To fill this gap,we report new data here concerning charcoal analysis from 24 archaeological sites dated 5200–2300 a BP in the NETP,where this period saw remarkable environmental and social changes.By coupling the results with published paleoenvironment records(especially pollen),we aim to explore the utilization of wood and its spatial-temporal changes,as well as the anthropogenic impacts on past forest dynamics in the NETP.The results suggest that the wood resources collected by humans were mainly needleleaf trees(Picea L.and Pinus L.),broadleaved trees(Populus L.,Salix L.,and Betula)as well as shrubs(Hippophae L.and Tamarix L.),and that these were distributed around the human habitation areas.There was a clear spatial difference in the woody assemblage collected by humans in the period 3600–2300 a BP.This was likely related to the diversity of natural environments in local areas,but there was an abnormal spatial pattern of forest composition with broadleaved trees dominant above 2500 masl caused not by climate change,but by the rapid increase of human settlement and wood collection in high altitude areas of the NETP since 3600 a BP.展开更多
文摘[Objective]The paper was to investigate the mechanism of Bacillus coagulans preparation in improving production performance of laying hens in late period of laying.[Method]A total of 648 individuals of"Jingfen 1"laying hens(66 weeks of age)in late period of laying were randomly divided into four groups,six replicates each group,and each group had 27 individuals of laying hens.The laying hens in control group were fed with basal diet,and those in experimental groupsⅠ,Ⅱ and Ⅲ were fed with the basal diets added with 3.33×10^6,1×10^7,3.33×10^7 CFU/g B.coagulans,respectively.The pretrial lasted one week,and the formal test lasted eight weeks.[Result]Compared with the control group,the laying rate in group Ⅰ increased significantly,and the feed-gain ratio in experimental group Ⅱ decreased noticeably while the spleen index increased remarkably;the alkaline phosphatase activities and blood calcium content in experimental groups Ⅰ,Ⅱ and Ⅲ increased significantly(P<0.05),while the triglyceride content decreased remarkably(P<0.05);the urea content in experimental groups Ⅰ and Ⅱ decreased obviously(P<0.05).Adding B.coagulans significantly increased the specific activity of amylase,lipase and protease in various intestinal mucosa of small intestine(P<0.05);adding B.coagulans significantly increased the content of follicle-stimulating hormone(FSH)and estradiol(E2),and significantly increased the mRNA expression of follicle stimulating hormone receptor gene(FSHR).[Conclusion]B.coagulans preparation could significantly improve the production performance of laying hens in late period of laying;appropriately enhance the immune capacity of laying hens;improve the serum biochemical indicators;increase the activity of digestive enzymes in the small intestine;and promote the release of gonadal hormone and the mRNA expression of FSHR gene in the ovary.
基金This work was supported by the National Natural Science Foundation of China(41702124,41772272)the China Geological Survey Program(DD20190094).
文摘The widely-developed,mixed clastic-carbonate succession in the northern Qaidam Basin records the paleo-environment changes under the glacial activity during the Late Paleozoic icehouse period in the context of regional tectonic stability,however,the depositional environment and sequence stratigraphy characteristics of the mixed deposits is rarely reported and still not clear.Combined the latest drilling wells data,we analyzed the sedimentary and stratigraphic characterization of the mixed strata via detailed field outcrops and core observations and thin section microscopic observations and recognized three depositional systems,including progradational coastal system,incised valley system,and carbonate-dominated marine shelf system,and identified four third-order sequences,SQ1,SQ2,SQ3 and SQ4,consisting of LST,TST,and HST.The depositional environment is overall belonged to marine-continental transition context and shifted from marine to continental environment frequently,showing an evolutionary pattern from marine towards terrestrial-marine transition and then back into the marine environment again in the long-term,which was controlled by the regional tectonic subsidence and the high-frequency and large-amplitude sea-level changes due to the Late Paleozoic glacial activity.The result is of significance in understanding the evolution of the Qinghai-Tibet Plateau and the sedimentation-climate response.
基金supported by the Strategic Priority Research Program of Chinese Academy of Sciences(XDA20040101)the Second Tibetan Plateau Scientific Expedition and Research Program(STEP)(2019QZKK0601)the National Natural Science Foundation of China(41825001,41671077).
文摘The relationship between the evolution of human societies and their living environments has been discussed intensively in recent decades.It is important to understand the patterns and mechanisms of human–environment interaction and evolution in order to cope with rapidly changing environments in the modern world.We reviewed the results of dating,archaeobotanical,and zooarchaeological studies from 139 prehistoric sites in the Northeast Tibetan Plateau(NETP)and contiguous regions(NETP-CR:i.e.,the Hexi Corridor and western margin of the Chinese Loess Plateau)and compared them with paleoenvironmental records to study variations in spatiotemporal patterns of social development in the area and their relationships with climate and vegetation changes.Our results show that hunter-gatherer groups occupied vast areas of the NETP at low intensities during^15,000–5500 BP(years before present).This was directly related to forest cover and climate change.An increase in temperature and precipitation turnover of vegetation from desert steppe to alpine meadow/subalpine shrub,and recovery of animal population on the Tibetan Plateau created more food resources and space for hunter-gatherers.Millet farmers settled extensively below 2500 m a.s.l.(meters above sea level)in the NETP-CR between^5500 and 3600 BP,and might have coexisted with hunting groups above 2500 m a.s.l.via subsistence exchange.The distribution of human settlements during that period was affected by climate change,with the relatively warm and wet climate promoting the expansion of millet agriculture to the NETP-CR during 5500–4500 BP,while climate deterioration caused humans to retreat to lower altitudes.During 3600–2200 BP,a range of livelihoods emerged in different regions of the NETPCR.This was promoted by early trans-Eurasian exchange and the development of an agro-pastoral economy that utilized cold-tolerant crops and livestock.This eventually promoted the expansion of permanent human settlements to high-altitude areas in the NETP.This study found that human societies adopted various strategies to adapt to the changing living environment throughout late prehistoric times in the NETP-CR.The results provide a long-term perspective on the trajectory of regional socio-environmental co-evolution.
基金Sponsored by Philosophical and Social Science Research Project in Colleges and Universities of Jiangsu Province in 2015(2015sjd751)
文摘During the late Qing Dynasty and the early period of the Republic of China(1870-1930),the social classes in Yangzhou were basically dominated by officials,businessmen,and scholars.In the building number,scale,and functional evolution of their home gardens,these three classes were different,reflecting abundant connotation of characteristic of the time and regional culture,revealing the inheritance path of Yangzhou culture,as well as the richness and diversity of home gardens in Yangzhou during the late Qing Dynasty and the early period of the Republic of China.
文摘As a world-level author,Shakespeare is more and more popular to Chinese readers.However,Shakespeare is hard to un⁃derstand for students who are not familiar to British history.To facilitate the understanding of Shakespeare’s plays,this paper intro⁃duces the historical background and historical events depicted in the works of Shakespeare and lists several themes discussed in Shakespeare’s history plays and tragedies.
文摘Objective To investigate the therapeutic efficacy of castration with 125 I brachtherapy in middle and late stage prostate cancer. Methods Sixty-six patients with prostate cancer from 2004 to 2009 were analyzed,40 were at clinical stage C and 26 were at clinical stage D,
基金supported by the National Key R&D Program of China(2017YFC0601304)the National Basic Research Program of China(also called 973 Program)(No.2013CB429802)the National Natural Science Foundation of China(NSFC)(No.41272094)
文摘The Yangbishan iron-tungsten deposit in the Shuangyashan area of Heilongjiang Province is located in the center of the Jiamusi Massif in northeastern China. The rare earth element and trace element compositions of the scheelite show that it formed in a reducing environment and inherited the rare earth element features of the ore-forming fluid. The geochemical characteristics of the gneissic granite associated with the tungsten mineralization show that the magma formed in this reducing environment and originated from the partial melting of metamorphosed shale that contained organic carbon and was enriched with tungsten. In addition, in situ Hf isotopic analysis of zircons from the gneissic granite indicates that they probably originated from the partial melting of a predominantly Paleo-Mesoproterozoic crustal source. According to LA-ICP-MS zircon dating, the Yangbishan ore- related gneissic granite has an Early Paleozoic crystallization age of 520.6 + 2.8 Ma. This study, together with previous data, indicates that the massifs of northeastern China, including Erguna, Xing'an, Songliao, Jiamusi, and Khanka massifs, belonged to an orogenic belt that existed along the southern margin of the Siberian Craton during the late Pan-African period. The significant continental movements of this orogeny resulted in widespread magmatic activity in northeastern China from 530 Ma to 470 Ma under a tectonic setting that transitioned from compressional syn-collision to extensional post- collision.
基金Supported by the China National Science and Technology Major Project (2016ZX05024-003)
文摘The Bohai Bay Basin is a typical oil-prone basin, in which natural gas geological reserves have a small proportion. In this basin, the gas source rock is largely medium-deep lake mudstone with oil-prone type Ⅱ2-Ⅱ1 kerogens, and natural gas preservation conditions are poor due to active late tectonic movements. The formation conditions of large natural gas fields in the Bohai Bay Basin have been elusive. Based on the exploration results of Bohai Bay Basin and comparison with large gas fields in China and abroad, the formation conditions of conventional large-scale natural gas reservoirs in the Bohai Bay Basin were examined from accumulation dynamics, structure and sedimentation. The results show that the formation conditions of conventional large natural gas reservoirs in Bohai Bay Basin mainly include one core element and two key elements. The core factor is the strong sealing of Paleogene "quilt-like" overpressure mudstone. The two key factors include the rapid maturation and high-intensity gas generation of source rock in the late stage and large scale reservoir. On this basis, large-scale nature gas accumulation models in the Bohai Bay Basin have been worked out, including regional overpressure mudstone enriching model, local overpressure mudstone depleting model, sand-rich sedimentary subsag depleting model and late strongly-developed fault depleting model. It is found that Bozhong sag, northern Liaozhong sag and Banqiao sag have favorable conditions for the formation of large-scale natural gas reservoirs, and are worth exploring. The study results have important guidance for exploration of large scale natural gas reservoirs in the Bohai Bay Basin.
文摘The blood neutrophils to lymphocytes ratio (NLR) reflects the physiological homeostasis between lymphopoiesis and myelopoiesis, and its elevation serves as a harmful sign in many pathologies, partially, late rejection of allograft. The stem and young lymphoid cells have regenerative-trophic properties, which can affect the relevance of NLR, being opposed to immune properties, associated with bulk lymphocytes. In the present article, we have analyzed for the first time the applicability of NLR’s analogs with stem and immature blood cells for monitoring harmful long-term shifting from lymphopoiesis to myelopoiesis in transplant’s recipients received conventional immunosuppressive treatment. In opposition to conventional NLR, the ratio of subpopulation of CD31 cells committed to the liver tissue by alfa-fetoprotein (AFP), seems sensitive enough for such monitoring several years after transplantation of the liver from the dead.
文摘Different arguments on the relationship between railway construction and economic development exist in literature. As the railway construction of Henan in the period of late Qing Dynasty(1644-1911) and early Republic of China(1912-1949) provides a nearly natural experiment to throw new light on this topic, this paper creates county-level panel data for this period and conducts a further test with a difference-in-difference strategy. While the effect of railway is demonstrated to be positive in some literature yet negative in others, this paper identifies some major intergroup differences: railway lines put into service after the 1910 s and connecting central cities promoted population growth in regions along the railway lines, enhanced their capacity to cope with external market shocks, yet they did not improve integration with the intra-provincial market. By contrast, the railway lines put into operation in the 1930 s and connecting hinterland areas not only improved regional market integration but contributed to higher living standards as well. The implication is that the effects of railway are subject to the economic relations of various localities along the railway lines and the economic attributes of various sectors. thus railways cannot be generally defined to be positive or negative. This conclusion helps explain the disagreements in empirical studies and highlight the impact of railways on the regional economic structure.
基金supported by the National Natural Science Foundation of China(Grant Nos.41825001,41820104008)the Second Tibetan Plateau Scientific Expedition and Research Program(STEP)(Grant No.2019QZKK0601)。
文摘Human activities are usually considered to have had impacts on forest degradation and/or changes of forest composition in the past.The Tibetan Plateau,an environmentally sensitive area,has witnessed a high intensity of human activities since the late Neolithic,but the evidence is still incomplete concerning how human activities affected the environment,especially vegetation changes.This may result,in part,from the fact that research on prehistoric wood utilization by humans in the northeast Tibetan Plateau(NETP)is scarce,especially from the perspective of charcoal analysis,which is an archaeological index directly related to human activities.To fill this gap,we report new data here concerning charcoal analysis from 24 archaeological sites dated 5200–2300 a BP in the NETP,where this period saw remarkable environmental and social changes.By coupling the results with published paleoenvironment records(especially pollen),we aim to explore the utilization of wood and its spatial-temporal changes,as well as the anthropogenic impacts on past forest dynamics in the NETP.The results suggest that the wood resources collected by humans were mainly needleleaf trees(Picea L.and Pinus L.),broadleaved trees(Populus L.,Salix L.,and Betula)as well as shrubs(Hippophae L.and Tamarix L.),and that these were distributed around the human habitation areas.There was a clear spatial difference in the woody assemblage collected by humans in the period 3600–2300 a BP.This was likely related to the diversity of natural environments in local areas,but there was an abnormal spatial pattern of forest composition with broadleaved trees dominant above 2500 masl caused not by climate change,but by the rapid increase of human settlement and wood collection in high altitude areas of the NETP since 3600 a BP.