针对目标图像利用导向滤波算法进行图像抠图时参数需要根据不同图像进行人为设定的问题,提出了一种利用隐藏的支持向量机LSVM(latent support vector machine)自动设定参数的潜在半径优化的数字图像抠图算法.该方法首先是应用LSVM潜在...针对目标图像利用导向滤波算法进行图像抠图时参数需要根据不同图像进行人为设定的问题,提出了一种利用隐藏的支持向量机LSVM(latent support vector machine)自动设定参数的潜在半径优化的数字图像抠图算法.该方法首先是应用LSVM潜在性的思想,利用已知数据库模板训练输入目标图像的样本集,再利用样本集将导向图像和二值图像以不同半径进行分块并进行判定,确定半径值,从而能够自动产生合理参数.最后利用导向滤波器对图像进行抠图,从而优化抠图算法,最终实现抠图算法的智能化和灵活化.展开更多
作为一种非线性维数约减算法,高斯过程隐变量模型(Gaussian process latent variable model,GPLVM)由于其适合处理小样本、高维数据,因而在模式识别、计算机视觉等领域得到了广泛应用.基于此,提出一种基于改进GPLVM的SAR图像目标特征提...作为一种非线性维数约减算法,高斯过程隐变量模型(Gaussian process latent variable model,GPLVM)由于其适合处理小样本、高维数据,因而在模式识别、计算机视觉等领域得到了广泛应用.基于此,提出一种基于改进GPLVM的SAR图像目标特征提取及自动识别方法,其中利用改进的GPLVM进行特征提取,高斯过程分类进行目标识别.传统GPLVM使用共轭梯度法对似然函数进行优化,为避免梯度估值易受噪声干扰、步长对算法影响严重等缺点,提出基于免疫克隆选择算法的GPLVM,利用其具有快速收敛到全局最优的特性提高算法性能.实验结果表明,该算法不仅降低了特征维数,且提高了识别精度,从而验证了算法用于SAR图像目标识别的有效性.展开更多
A preliminary screening for garlic viruses in garlic plants in Hunan,China,using existing monoplex（simplex） reverse transcription-polymerase chain reaction（RT-PCR） procedures detected four viruses/virus groups.The...A preliminary screening for garlic viruses in garlic plants in Hunan,China,using existing monoplex（simplex） reverse transcription-polymerase chain reaction（RT-PCR） procedures detected four viruses/virus groups.These viruses/virus groups were Onion yellow dwarf virus（OYDV）,Leek yellow stripe virus（LYSV）,Shallot latent virus（SLV） and allexiviruses（e.g.,garlic viruses A,B,C,D,E,X）.Sequence analysis of the projected allexivirus amplicons revealed the allexivirus in the infected garlic plants was Garlic virus D（GarV-D）,which shared 92-97%sequence identities with various isolates from the world.A multiplex RT-PCR（mRT-PCR） was therefore developed to simultaneously detect and differentiate the four viruses/virus groups.To achieve this,four primer pairs targeting allexiviruses,OYDV,LYSV and SLV were designed.The anticipated amplicon sizes are 183 bp（allexiviruses）,265 bp（OYDV）,404 bp（LYSV） and 592 bp（SLV）,respectively.All primer pairs produced virus-specific fragments in both simplex and multiplex formats,thus confirming the efficacy of the newly developed mRT-PCR for detection of these viruses.The mRT-PCR further was evaluated by applying it to garlic plant samples collected in two geographic locations in Hunan.Allexiviruses,OYDV,LYSV and SLV were detected in 50.9,40.3,28.3 and 58.5%of leaf samples,respectively;and mixed infections with two or more viruses accounted for 54%of the garlic samples.The results obtained by mRT-PCR were confirmed by simplex RT-PCR assays.In conclusion,this newly developed mRT-PCR provides a rapid,sensitive and reliable method for the detection and identification of major garlic viruses.展开更多
目的:探讨EB病毒(Epstein-Barr virus,EBV)潜伏膜蛋白1(Latent membrane protein 1,LMP1)基因特异性的脱氧核梅(DNAzyme)对伯基特淋巴瘤细胞P3HR-1的增殖和凋亡的影响。方法:设计合成针对EBV-LMP1基因的DNAzyme(DZ1),在P3HR-1细胞上观...目的:探讨EB病毒(Epstein-Barr virus,EBV)潜伏膜蛋白1(Latent membrane protein 1,LMP1)基因特异性的脱氧核梅(DNAzyme)对伯基特淋巴瘤细胞P3HR-1的增殖和凋亡的影响。方法:设计合成针对EBV-LMP1基因的DNAzyme(DZ1),在P3HR-1细胞上观察其对细胞增殖活性、细胞周期和细胞凋亡的影响。结果:脱氧核酶DZ1作用于P3HR-1细胞后,可显著抑制EBV-LMP1基因的表达,并呈剂量依赖性。将脱氧核酶DZ1抑制LMP1的表达,进而下调抗凋亡蛋白Bc1-2的表达,诱导细胞色素c释放,从而诱导细胞凋亡。结论:在P3HR-1细胞中,靶向LMP1可能成为潜在的基因治疗的分子靶,靶向LMP1的脱氧核酶可能成为EB病毒相关肿瘤基因治疗实验研究的新的工具。展开更多
文摘针对目标图像利用导向滤波算法进行图像抠图时参数需要根据不同图像进行人为设定的问题,提出了一种利用隐藏的支持向量机LSVM(latent support vector machine)自动设定参数的潜在半径优化的数字图像抠图算法.该方法首先是应用LSVM潜在性的思想,利用已知数据库模板训练输入目标图像的样本集,再利用样本集将导向图像和二值图像以不同半径进行分块并进行判定,确定半径值,从而能够自动产生合理参数.最后利用导向滤波器对图像进行抠图,从而优化抠图算法,最终实现抠图算法的智能化和灵活化.
文摘作为一种非线性维数约减算法,高斯过程隐变量模型(Gaussian process latent variable model,GPLVM)由于其适合处理小样本、高维数据,因而在模式识别、计算机视觉等领域得到了广泛应用.基于此,提出一种基于改进GPLVM的SAR图像目标特征提取及自动识别方法,其中利用改进的GPLVM进行特征提取,高斯过程分类进行目标识别.传统GPLVM使用共轭梯度法对似然函数进行优化,为避免梯度估值易受噪声干扰、步长对算法影响严重等缺点,提出基于免疫克隆选择算法的GPLVM,利用其具有快速收敛到全局最优的特性提高算法性能.实验结果表明,该算法不仅降低了特征维数,且提高了识别精度,从而验证了算法用于SAR图像目标识别的有效性.
文摘局部放电监测是实现气体绝缘组合电器(gas insulated switchgear,GIS)缺陷预警的重要依据和手段。DL/T639—1997《六氟化硫电气设备运行、试验及检修人员安全防护细则》等化学诊断法规程将S_2OF_(10)作为一种GIS局部放电特征气体列入常规检测范围。为探究S_2OF_(10)作为局部放电特征气体的有效性,在110k V GIS试验平台上设置典型放电模型,研究不同放电类型和放电程度下S_2OF_(10)随时间的变化规律,并通过B3P86/6-31G(d,p)量子化学计算揭示了S_2OF_(10)的生成机理。试验和理论计算均表明,S_2OF_(10)由自由基SF5、SF5O结合生成,放电状态下只有极少量的SF5、SF5O生成,且二者共存机会很小,GIS内不同放电类型和能量条件下,S_2OF_(10)均不会大量生成。结合对运行GIS放电气室气体组分分析结果,S_2OF_(10)不能作为一种有效的局部放电特征气体用于GIS潜伏性缺陷诊断。
基金supported by the Non-Profit Industry Financial Program of Ministry of Agriculture of China(20130328)to Prof.Liu Yong and Prof.Dai Liangyingthe Natural Science Foundation of Hunan Province,China to Prof.Hu Xinxi(11JJ2018)
文摘A preliminary screening for garlic viruses in garlic plants in Hunan,China,using existing monoplex（simplex） reverse transcription-polymerase chain reaction（RT-PCR） procedures detected four viruses/virus groups.These viruses/virus groups were Onion yellow dwarf virus（OYDV）,Leek yellow stripe virus（LYSV）,Shallot latent virus（SLV） and allexiviruses（e.g.,garlic viruses A,B,C,D,E,X）.Sequence analysis of the projected allexivirus amplicons revealed the allexivirus in the infected garlic plants was Garlic virus D（GarV-D）,which shared 92-97%sequence identities with various isolates from the world.A multiplex RT-PCR（mRT-PCR） was therefore developed to simultaneously detect and differentiate the four viruses/virus groups.To achieve this,four primer pairs targeting allexiviruses,OYDV,LYSV and SLV were designed.The anticipated amplicon sizes are 183 bp（allexiviruses）,265 bp（OYDV）,404 bp（LYSV） and 592 bp（SLV）,respectively.All primer pairs produced virus-specific fragments in both simplex and multiplex formats,thus confirming the efficacy of the newly developed mRT-PCR for detection of these viruses.The mRT-PCR further was evaluated by applying it to garlic plant samples collected in two geographic locations in Hunan.Allexiviruses,OYDV,LYSV and SLV were detected in 50.9,40.3,28.3 and 58.5%of leaf samples,respectively;and mixed infections with two or more viruses accounted for 54%of the garlic samples.The results obtained by mRT-PCR were confirmed by simplex RT-PCR assays.In conclusion,this newly developed mRT-PCR provides a rapid,sensitive and reliable method for the detection and identification of major garlic viruses.
文摘目的:探讨EB病毒(Epstein-Barr virus,EBV)潜伏膜蛋白1(Latent membrane protein 1,LMP1)基因特异性的脱氧核梅(DNAzyme)对伯基特淋巴瘤细胞P3HR-1的增殖和凋亡的影响。方法:设计合成针对EBV-LMP1基因的DNAzyme(DZ1),在P3HR-1细胞上观察其对细胞增殖活性、细胞周期和细胞凋亡的影响。结果:脱氧核酶DZ1作用于P3HR-1细胞后,可显著抑制EBV-LMP1基因的表达,并呈剂量依赖性。将脱氧核酶DZ1抑制LMP1的表达,进而下调抗凋亡蛋白Bc1-2的表达,诱导细胞色素c释放,从而诱导细胞凋亡。结论:在P3HR-1细胞中,靶向LMP1可能成为潜在的基因治疗的分子靶,靶向LMP1的脱氧核酶可能成为EB病毒相关肿瘤基因治疗实验研究的新的工具。
