ObjectiveTo study the effects of dendritic cells (DC) transfected with recombinant vaccinia virus encoding Epstein Barr virus (EBV) latent membrane protein 2A(LMP2A) gene,and to provide evidence for further investiga...ObjectiveTo study the effects of dendritic cells (DC) transfected with recombinant vaccinia virus encoding Epstein Barr virus (EBV) latent membrane protein 2A(LMP2A) gene,and to provide evidence for further investigation on the therapeutic vaccines against EBV associated malignancies. MethodsMature DC were transfected with EBV LMP2A recombinant vaccinia virus (rVV LMP2A). Before and after the transfection,the expression of surface antigens on mature DC including CD1a,CD83,CD40,CD80,HLA DR was measured by fluorescence activated cell sorter (FACS) and the function of DC to stimulate allogeneic T cells proliferation was measured by mixed leukocyte reactions (MLR). ResultsLMP2A protein was highly expressed (66.1 %) in DC after the transfection of rVV LMP2A. No significant changes in the primary surface antigens expression and in the MLR were detected during the transfection. Transfected DC still had strong potential in stimulating the proliferation of allogeneic T cells. ConclusionRecombinant vaccinia virus was an effective and non perturbing vector to mediate the transfection of LMP2A into DC. The functions of mature DC were not affected significantly by the transfection of Vac LMP2A. This study could provide evidence for the further immunotherapy of EBV associated malignancies,e.g. nasopharyngeal carcinoma (NPC).展开更多
The quasi-geostrophic Q vector is an important rainfall associated with large-scale weather systems diagnostic tool for studying development of surface and is calculated using data at single vertical level. When ageos...The quasi-geostrophic Q vector is an important rainfall associated with large-scale weather systems diagnostic tool for studying development of surface and is calculated using data at single vertical level. When ageostrophic Q vector was introduced, it required data at two vertical levels. In this study, moist ageostrophic Q vector is modified so that it can be calculated using data at a single vertical level. The comparison study between the original and modified moist ageostrophic Q vectors is conducted using the data from 5 to 6 July 1991 during the torrential rainfall event associated with the Changjiang-Huaihe mei-yu front in China. The results reveal that divergences of original and modified moist ageostrophic Q vectors have similar horizontal distributions and their centers are almost located in the precipitation centers. This indicates that modified moist ageostrophic Q vector can be used to diagnose convective development with reasonable accuracy.展开更多
In recent years,multimedia annotation problem has been attracting significant research attention in multimedia and computer vision areas,especially for automatic image annotation,whose purpose is to provide an efficie...In recent years,multimedia annotation problem has been attracting significant research attention in multimedia and computer vision areas,especially for automatic image annotation,whose purpose is to provide an efficient and effective searching environment for users to query their images more easily. In this paper,a semi-supervised learning based probabilistic latent semantic analysis( PLSA) model for automatic image annotation is presenred. Since it's often hard to obtain or create labeled images in large quantities while unlabeled ones are easier to collect,a transductive support vector machine( TSVM) is exploited to enhance the quality of the training image data. Then,different image features with different magnitudes will result in different performance for automatic image annotation. To this end,a Gaussian normalization method is utilized to normalize different features extracted from effective image regions segmented by the normalized cuts algorithm so as to reserve the intrinsic content of images as complete as possible. Finally,a PLSA model with asymmetric modalities is constructed based on the expectation maximization( EM) algorithm to predict a candidate set of annotations with confidence scores. Extensive experiments on the general-purpose Corel5k dataset demonstrate that the proposed model can significantly improve performance of traditional PLSA for the task of automatic image annotation.展开更多
To look for a more stable and convenient way of constructing short hairpin RNA expression vectors targeting the latent membrane protein-1(LMP-1)encoded by Epstein-Barr virus(pshLMP1),and to study the inhibition functi...To look for a more stable and convenient way of constructing short hairpin RNA expression vectors targeting the latent membrane protein-1(LMP-1)encoded by Epstein-Barr virus(pshLMP1),and to study the inhibition function of pshLMP1 expression vectors in HNE1 cells,we designed the pshLMP1 expression cassette and pshLMP1 expression vectors by both the annealing method and PCR method and then co-transfected with pEGFP-N1-1158 into HNE1 cells to observe the mRNA and protein levels of LMP-1 genes by green fluorescence analysis,RT-PCR and western blot.pshLMP1 expression vectors were successfully obtained by both methods but better cloning efficiency was achieved and fewer deletions and mutations of nucleotides were achieved with the PCR method.Furthermore,the mRNA and protein levels of LMP-1 genes were down-regulated by pshLMP1 expression vectors.According to our research,we found that the PCR method provides a more efficient way to construct pshLMP1 expression vectors which have the ability to inhibit the function of LMP-1 genes expressed in HNE1 cells,and also provides a novel application of RNA interference technology against-EBV.展开更多
针对传统基于梯度方向直方图特征检测算法对解决目标模型单一、发生形变、存在遮挡及目标受干扰下定位困难的问题,提出一种基于HOG特征混合模型结合隐SVM的感兴趣目标检测算法。首先利用用训练图像的HOG特征金字塔表示得到包含感兴趣目...针对传统基于梯度方向直方图特征检测算法对解决目标模型单一、发生形变、存在遮挡及目标受干扰下定位困难的问题,提出一种基于HOG特征混合模型结合隐SVM的感兴趣目标检测算法。首先利用用训练图像的HOG特征金字塔表示得到包含感兴趣目标根模型、部件模型和对应可变形部件特征表示,该模型不仅描述目标的整体轮廓,而且能够捕捉到更为精细的目标部件轮廓,在一定程度上提高了检测算法在目标姿态复杂情况下的鲁棒性。然后利用HOG特征混合特征训练部件检测分类器LSVM(Latent Support Vector Machine)。最后通过动态规划和距离转换算法在测试图上扫描出与可变形部件模型相匹配的区域,实现感兴趣目标的检测定位。经过多组实验结果表明,所提出的算法能较好地解决目标在发生较大形变和存在遮挡等复杂姿态下的定位问题。展开更多
基金This paper is supported by grant from the National Natural Science Foundation of China(No.30 1 70 880 )
文摘ObjectiveTo study the effects of dendritic cells (DC) transfected with recombinant vaccinia virus encoding Epstein Barr virus (EBV) latent membrane protein 2A(LMP2A) gene,and to provide evidence for further investigation on the therapeutic vaccines against EBV associated malignancies. MethodsMature DC were transfected with EBV LMP2A recombinant vaccinia virus (rVV LMP2A). Before and after the transfection,the expression of surface antigens on mature DC including CD1a,CD83,CD40,CD80,HLA DR was measured by fluorescence activated cell sorter (FACS) and the function of DC to stimulate allogeneic T cells proliferation was measured by mixed leukocyte reactions (MLR). ResultsLMP2A protein was highly expressed (66.1 %) in DC after the transfection of rVV LMP2A. No significant changes in the primary surface antigens expression and in the MLR were detected during the transfection. Transfected DC still had strong potential in stimulating the proliferation of allogeneic T cells. ConclusionRecombinant vaccinia virus was an effective and non perturbing vector to mediate the transfection of LMP2A into DC. The functions of mature DC were not affected significantly by the transfection of Vac LMP2A. This study could provide evidence for the further immunotherapy of EBV associated malignancies,e.g. nasopharyngeal carcinoma (NPC).
基金National Natural Science Foundation of China (Grant Nos. 40405009, 40075009, 40205008)Shanghai Typhoon Research Funding (Grant No. 2003ST005)+1 种基金Shanghai Weather Bureau Research Funding (No. 04A06)Jiangsu Key Laboratory of Meteorological Disaster Fund- ing (No. KJS0602)
文摘The quasi-geostrophic Q vector is an important rainfall associated with large-scale weather systems diagnostic tool for studying development of surface and is calculated using data at single vertical level. When ageostrophic Q vector was introduced, it required data at two vertical levels. In this study, moist ageostrophic Q vector is modified so that it can be calculated using data at a single vertical level. The comparison study between the original and modified moist ageostrophic Q vectors is conducted using the data from 5 to 6 July 1991 during the torrential rainfall event associated with the Changjiang-Huaihe mei-yu front in China. The results reveal that divergences of original and modified moist ageostrophic Q vectors have similar horizontal distributions and their centers are almost located in the precipitation centers. This indicates that modified moist ageostrophic Q vector can be used to diagnose convective development with reasonable accuracy.
基金Supported by the National Program on Key Basic Research Project(No.2013CB329502)the National Natural Science Foundation of China(No.61202212)+1 种基金the Special Research Project of the Educational Department of Shaanxi Province of China(No.15JK1038)the Key Research Project of Baoji University of Arts and Sciences(No.ZK16047)
文摘In recent years,multimedia annotation problem has been attracting significant research attention in multimedia and computer vision areas,especially for automatic image annotation,whose purpose is to provide an efficient and effective searching environment for users to query their images more easily. In this paper,a semi-supervised learning based probabilistic latent semantic analysis( PLSA) model for automatic image annotation is presenred. Since it's often hard to obtain or create labeled images in large quantities while unlabeled ones are easier to collect,a transductive support vector machine( TSVM) is exploited to enhance the quality of the training image data. Then,different image features with different magnitudes will result in different performance for automatic image annotation. To this end,a Gaussian normalization method is utilized to normalize different features extracted from effective image regions segmented by the normalized cuts algorithm so as to reserve the intrinsic content of images as complete as possible. Finally,a PLSA model with asymmetric modalities is constructed based on the expectation maximization( EM) algorithm to predict a candidate set of annotations with confidence scores. Extensive experiments on the general-purpose Corel5k dataset demonstrate that the proposed model can significantly improve performance of traditional PLSA for the task of automatic image annotation.
文摘To look for a more stable and convenient way of constructing short hairpin RNA expression vectors targeting the latent membrane protein-1(LMP-1)encoded by Epstein-Barr virus(pshLMP1),and to study the inhibition function of pshLMP1 expression vectors in HNE1 cells,we designed the pshLMP1 expression cassette and pshLMP1 expression vectors by both the annealing method and PCR method and then co-transfected with pEGFP-N1-1158 into HNE1 cells to observe the mRNA and protein levels of LMP-1 genes by green fluorescence analysis,RT-PCR and western blot.pshLMP1 expression vectors were successfully obtained by both methods but better cloning efficiency was achieved and fewer deletions and mutations of nucleotides were achieved with the PCR method.Furthermore,the mRNA and protein levels of LMP-1 genes were down-regulated by pshLMP1 expression vectors.According to our research,we found that the PCR method provides a more efficient way to construct pshLMP1 expression vectors which have the ability to inhibit the function of LMP-1 genes expressed in HNE1 cells,and also provides a novel application of RNA interference technology against-EBV.
文摘针对传统基于梯度方向直方图特征检测算法对解决目标模型单一、发生形变、存在遮挡及目标受干扰下定位困难的问题,提出一种基于HOG特征混合模型结合隐SVM的感兴趣目标检测算法。首先利用用训练图像的HOG特征金字塔表示得到包含感兴趣目标根模型、部件模型和对应可变形部件特征表示,该模型不仅描述目标的整体轮廓,而且能够捕捉到更为精细的目标部件轮廓,在一定程度上提高了检测算法在目标姿态复杂情况下的鲁棒性。然后利用HOG特征混合特征训练部件检测分类器LSVM(Latent Support Vector Machine)。最后通过动态规划和距离转换算法在测试图上扫描出与可变形部件模型相匹配的区域,实现感兴趣目标的检测定位。经过多组实验结果表明,所提出的算法能较好地解决目标在发生较大形变和存在遮挡等复杂姿态下的定位问题。
