Using SRAP(sequence-related amplified polymorphism)markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F_(2) individuals derived from a cross of the two cucumber lines,S06 and...Using SRAP(sequence-related amplified polymorphism)markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F_(2) individuals derived from a cross of the two cucumber lines,S06 and S52.In the survey of parental polymorphisms with 182 primer combinations,64 polymorphism-revealing primer pairs were screened out,which generated totally 108 polymorphic bands with an average of 1.7 bands per primer pair and at most 6 bands from one primer pair.The constructed molecular linkage map included 92 loci,distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of 12.6 cM between two neighboring loci.Based on this linkage map,the quantitative trait loci(QTL)for the lateral branch number(lbn)and the lateral branch average length(lbl)in cucumber were identified by QTLMapper1.6.A major QTL lbn1 located between ME11SA4B and ME5EM5 in LG2 could explain 10.63%of the total variation with its positively effecting allele from S06.A major QTL lbl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38%of the total variation with its positively effecting allele from S_(06).展开更多
Inorder to carry out trunk extension by lateral buds ,the laws of trunk extension and branching sprouted from lateral buds were investigated in 1-year-old seedlings of P.tomentosaxP,fortunei 33 under natural condition...Inorder to carry out trunk extension by lateral buds ,the laws of trunk extension and branching sprouted from lateral buds were investigated in 1-year-old seedlings of P.tomentosaxP,fortunei 33 under natural conditions.The results indicated：（1）Under conditions of high density and extensive management,42%of individuals in P.tomentosaxP.fortunei 33 showed pseudo dichotomous branching characteristics and could not extend their trunks in the second year.But other individuals showed the characteristics of sympodial branching and pseudo monopodial branching （not from terminal buds ）and could realize natural trunk extension.All the 3types of branching could be called branching of pseudo asis;（2）The stem form quotieties of extended trunk in all individuals wrer larger in the second year ,but only 14%of them could straight extend their trunks and 86%of them needed2-3years to reach straight trunk estension;（3）Among individuals which could extend their trunks ,from upper lateral buds to lower lateral buds on trunks ,the lengths and diameters of branches sprouted from lateral buds became smaller and smaller ,but the angles of branching up to trunk became larger and larger ,and the fourth pair of lateral branches reached to the largest,Under the fourth pair ,the angles of branches became smaller again and those branches all died.展开更多
A group of 224 recombinant inbred lines (RILs) was derived from a narrow cross between 2 cucumber (Cucumis sativus L.) lines, namely, S94 (Northern China type with weak lateral branch growth potential and early latera...A group of 224 recombinant inbred lines (RILs) was derived from a narrow cross between 2 cucumber (Cucumis sativus L.) lines, namely, S94 (Northern China type with weak lateral branch growth potential and early lateral branch sprouting time) and S06 (Northern European type with strong lateral branch growth potential and late lateral branch sprouting time). These lines were then used for investigating lateral branch-related traits. A total of 36 quantitative trait loci (QTLs) were detected for the following 4 lateral branch-related traits: lateral branch average length (LBAL), lateral branch total length (LBTL), lateral branch number (LBN), and first lateral branch node (FLBN). Further, each QTL explained 3.1% (lbtl2.1, spring) to 32.3% (lbn2.3, spring) of the observed phenotypic variance. Eleven QTLs (lbal1.1, lbtl1.1, lbn1.2, flbn1.2, etc.) for different traits were found to be clustered on the e23m18d-ME23EM6c section (7.4 cM) of linkage group (LG) 1; further, 15 QTLs (lbal2.1, lbtl2.1, lbn2.1, flbn2.1, etc.) were found to be clustered on the S94A1-ME4SA4a section (13.9 cM) of LG2. Twenty-one QTLs explained more than 10% of the phenotypic variance. Moreover, lbtl1.3 (autumn, 26.2%, logarithm of odds (LOD) = 17.4; spring, 26.9%, LOD = 17.9) had stable position and contribution in both seasons. Several se-quence-anchor markers (CMBR40, F, CS30, S94A1, CSWTA11B, etc.) were closely linked with some QTLs for LBAL, LBTL, LBN, and FLBN, which can be used for the marker-assisted selection to improve the plant architecture in cucumber breeding.展开更多
基金This work was support ed by the Shanghai Municipal Scientifi c&Technological Commission(Grant Nos.033107019)Shanghai Municipal Agricultural Commission(Grant Nos 2003-2-1-2).
文摘Using SRAP(sequence-related amplified polymorphism)markers a genetic linkage map of cucumber was constructed with a population consisting of 138 F_(2) individuals derived from a cross of the two cucumber lines,S06 and S52.In the survey of parental polymorphisms with 182 primer combinations,64 polymorphism-revealing primer pairs were screened out,which generated totally 108 polymorphic bands with an average of 1.7 bands per primer pair and at most 6 bands from one primer pair.The constructed molecular linkage map included 92 loci,distributed in seven linkage groups and spanning 1164.2 cM in length with an average genetic distance of 12.6 cM between two neighboring loci.Based on this linkage map,the quantitative trait loci(QTL)for the lateral branch number(lbn)and the lateral branch average length(lbl)in cucumber were identified by QTLMapper1.6.A major QTL lbn1 located between ME11SA4B and ME5EM5 in LG2 could explain 10.63%of the total variation with its positively effecting allele from S06.A major QTL lbl1 located between DC1OD3 and DC1EM14 in LG2 could account for 10.38%of the total variation with its positively effecting allele from S_(06).
基金Foundation of Research Startup for Returned Student From Abroad Science Foundation of Henan Province forDistinguished Young Scholars(No.002).
文摘Inorder to carry out trunk extension by lateral buds ,the laws of trunk extension and branching sprouted from lateral buds were investigated in 1-year-old seedlings of P.tomentosaxP,fortunei 33 under natural conditions.The results indicated：（1）Under conditions of high density and extensive management,42%of individuals in P.tomentosaxP.fortunei 33 showed pseudo dichotomous branching characteristics and could not extend their trunks in the second year.But other individuals showed the characteristics of sympodial branching and pseudo monopodial branching （not from terminal buds ）and could realize natural trunk extension.All the 3types of branching could be called branching of pseudo asis;（2）The stem form quotieties of extended trunk in all individuals wrer larger in the second year ,but only 14%of them could straight extend their trunks and 86%of them needed2-3years to reach straight trunk estension;（3）Among individuals which could extend their trunks ,from upper lateral buds to lower lateral buds on trunks ,the lengths and diameters of branches sprouted from lateral buds became smaller and smaller ,but the angles of branching up to trunk became larger and larger ,and the fourth pair of lateral branches reached to the largest,Under the fourth pair ,the angles of branches became smaller again and those branches all died.
基金Shanghai Leading Academic Discipline Project(Grant No.B209)
文摘A group of 224 recombinant inbred lines (RILs) was derived from a narrow cross between 2 cucumber (Cucumis sativus L.) lines, namely, S94 (Northern China type with weak lateral branch growth potential and early lateral branch sprouting time) and S06 (Northern European type with strong lateral branch growth potential and late lateral branch sprouting time). These lines were then used for investigating lateral branch-related traits. A total of 36 quantitative trait loci (QTLs) were detected for the following 4 lateral branch-related traits: lateral branch average length (LBAL), lateral branch total length (LBTL), lateral branch number (LBN), and first lateral branch node (FLBN). Further, each QTL explained 3.1% (lbtl2.1, spring) to 32.3% (lbn2.3, spring) of the observed phenotypic variance. Eleven QTLs (lbal1.1, lbtl1.1, lbn1.2, flbn1.2, etc.) for different traits were found to be clustered on the e23m18d-ME23EM6c section (7.4 cM) of linkage group (LG) 1; further, 15 QTLs (lbal2.1, lbtl2.1, lbn2.1, flbn2.1, etc.) were found to be clustered on the S94A1-ME4SA4a section (13.9 cM) of LG2. Twenty-one QTLs explained more than 10% of the phenotypic variance. Moreover, lbtl1.3 (autumn, 26.2%, logarithm of odds (LOD) = 17.4; spring, 26.9%, LOD = 17.9) had stable position and contribution in both seasons. Several se-quence-anchor markers (CMBR40, F, CS30, S94A1, CSWTA11B, etc.) were closely linked with some QTLs for LBAL, LBTL, LBN, and FLBN, which can be used for the marker-assisted selection to improve the plant architecture in cucumber breeding.
