Data analysis guidelines for single‑cell RNA‑seq in biomedical studies and clinical applications

The application of single-cell RNA sequencing(scRNA-seq)in biomedical research has advanced our understanding of the pathogenesis of disease and provided valuable insights into new diagnostic and therapeutic strategies.With the expansion of capacity for high-throughput scRNA-seq,including clinical samples,the analysis of these huge volumes of data has become a daunting prospect for researchers entering this field.Here,we review the workflow for typical scRNA-seq data analysis,covering raw data processing and quality control,basic data analysis applicable for almost all scRNA-seq data sets,and advanced data analysis that should be tailored to specific scientific questions.While summarizing the current methods for each analysis step,we also provide an online repository of software and wrapped-up scripts to support the implementation.Recommendations and caveats are pointed out for some specific analysis tasks and approaches.We hope this resource will be helpful to researchers engaging with scRNA-seq,in particular for emerging clinical applications.

Application Analysis of Cardiac Rehabilitation Nursing in Patients with Acute Myocardial Infarction

Objective: Application analysis of cardiac rehabilitation in patients with acute myocardial infarction. Methods: A total of 102 patients with acute myocardial infarction admitted to our hospital from January 2020 to January 2022 were retrospectively selected as the observation objects. According to the nursing measures taken, they were divided into a control group and an observation group, with 51 cases in each group. A total of 51 patients in the control group received routine nursing, while 51 patients in the observation group were guided to actively participate in the cardiac rehabilitation nursing plan. The hospitalization conditions and effects of the two groups were compared. Results: The cardiac function index, bed time, hospitalization time, anxiety score and depression score of the observation group were significantly better than those of the control group, and the differences were statistically significant (P Conclusion: The application of cardiac rehabilitation nursing in patients with acute myocardial infarction has a good effect. The patients’ cardiac function recovers faster, they feel more comfortable, and they also reduce the time of hospitalization and bed rest, which is conducive to improving the psychological and anxiety state of patients and improving the quality of life.

Finite Element Simulation Analysis of a Novel 3D-FRSPA for Crawling Locomotion

A novel three-dimensional-fiber reinforced soft pneumatic actuator(3D-FRSPA)inspired by crab claw and human hand structure that can bend and deform independently in each segment is proposed.It has an omni-directional bending configuration,and the fibers twined symmetrically on both sides to improve the bending performance of FRSPA.In this paper,the static and kinematic analysis of 3D-FRSPA are carried out in detail.The effects of fiber,pneumatic chamber and segment length,and circular air chamber radius of 3D-FRSPA on the mechanical performance of the actuator are discussed,respectively.The soft mobile robot composed of 3D-FRSPA has the ability to crawl.Finally,the crawling processes of the soft mobile robot on different road conditions are studied,respectively,and the motion mechanism of the mobile actuator is shown.The numerical results show that the soft mobile robots have a good comprehensive performance,which verifies the correctness of the proposedmodel.This work shows that the proposed structures have great potential in complex road conditions,unknown space detection and other operations.

Correction:Nonlinear dynamics in Divisia monetary aggregates:an application of recurrence quantification analysis

Following publication of the original article(Andreadis et al.2023),the authors reported that they mistakenly omitted the affiliations 1,2 and 3 for Ioannis Andreadis,Athanasios D.Fragkou and Theodoros E.Karakasidis.The correct affiliations for each author should read.

Brief analysis on the application of nanocellulose in textile field

Nowadays,the application of green,renewable and sustainable materials has become increasingly important for producing various high-value products with low environmental impact.Because such materials turn out to be an alternative solution to the ever-depleting nonrenewable sources,environmental pollution,global warming,and energy crisis.Nanocellulose is one of the most prominent green materials of modern times.

A New Method for Clay Mineral Analysis and Its Application in Geology

X-ray diffraction (XRD) peaks in a low-angle diffraction section of clayminerals, especially those of authigenic origin, have broadening and tailing features in shape.Using the five basic parameters, peak position, peak height, width, shape coefficient and asymmetry,to describe an XRD peak is more accurate, comprehensive and integrated than using only 3 of them,position, height and width. Following the concept of the five basic parameters of an XRD peak, theprogram Decoform proposed in this study provides more information in mineralogical analyses byfitting actual XRD profiles. In combination with the HW-IR plot, Decoform can he systematically andaccurately used in the comprehensive analyses of crystallinity, domain size, lattice strain andquantitative phase. It is also of value for the geological investigations of diagenesis,metamorphism, basin maturity, structural stress field and so on.

POWER FLOW ANALYSIS AND APPLICATION SIMULATION OF ELECTROMAGNETIC CONTINUOUSLY VARIABLE TRANSMISSION

With the structure of two air gaps and two rotors, the electromagnetic continuously variable transmission（EMCVT） is a novel power-split continuously variable transmission（CVT）. There are two kinds of power flowing through the EMCVT, one is mechanical power and the other is electric power. In the mean time, there are three power ports in the EMCVT, one is the outer rotor named mechanical power port and the other two are the inner rotor and the stator named electric power ports. The mechanical power port is connected to the driving wheels through the final gear and the electric ports are connected to the batteries through the transducers. The two kinds of power are coupled on the outer rotor of the EMCVT. The EMCVT can be equipped on the conventional vehicle being regarded as the CVT and it also can be equipped on the hybrid electric vehicle（HEV） as the multi-energy sources assembly. The power flows of these two kinds of applications are analysed. The back electromotive force（EMF） equations are illatively studied and so the dynamic mathematic model is theorized. In order to certify the feasibility of the above theories, three simulations are carried out in allusion to the above two kinds of mentioned applications of the EMCVT and a five speed automatic transmission（AT） vehicle. The simulation results illustrate that the efficiency of the EMCVT vehicles is higher than that of the AT vehicle owed to the optimized operation area of the engine. Hence the fuel consumption of the EMCVT vehicles is knock-down.

Architecture design and demand analysis on application layer of standard system for ubiquitous power Internet of Things

The ubiquitous power Internet of Things(UPIoT)is an intelligent service system with comprehensive state perception,efficient processing,and flexible application of information.It focuses on each link of the power system and makes full use of the mobile internet,artificial intelligence,and other advanced information and communication technologies in order to realize the inter-human interaction of all things in all links of the power system.This article systematically presents to the national and international organizations and agencies in charge of UPIoT layer standardization the status quo of the research on the Internet of Things(IoT)-related industry standards system.It briefly describes the generic standard classification methods,layered architecture,conceptual model,and system tables in the UPIoT application layer.Based on the principles of inheritance,innovation,and practicability,this study divides the application layer into customer service,power grid operation,integrated energy,and enterprise operation,emerging business and analyzes the standard requirements of these five fields.This study also proposes a standard plan.Finally,it summarizes the research report and provides suggestions for a follow-up work.

AN APPLICATION OF TOPOLOGICAL ANALYSIS TO STUDYING THE THREE-DIMENSIONAL FLOW IN CASCADES;PART I—TOPOLOGICAL RULES FOR SKIN-FRICTION LINES AND SECTION STREAMLINES

Based on the working of Lighthill and Hunt et al., in the present paper the author has established the topological rules adapting to analysing the skin-friction lines and the section streamlines in cascades. These rules are (1) for a rotor cascade without shroud band, the total number of nodal points equals that the saddle points on the skin-friction line vector fields in eachpitch range; (2) for an annular or straight cascade with no-clearances at blade ends, the total number of saddle points is two more than that of nodal points on the skin-friction line fields in a pitch; (3) the total number of saddles in the secondary flow fields on cross-sections in cascade is one less than that of nodes; (4) in the section streamline vector fields on a meridian surface penetrating a flow passage, and on leading and trailing edge sections, the total number of nodes is equal to that of saddles; (5) on the streamline vector fields of a blade-to-blade surface, the total number of nodes is one less than that of saddles.

Benchmarking Approach to Compare Web Applications Static Analysis Tools Detecting OWASP Top Ten Security Vulnerabilities

To detect security vulnerabilities in a web application,the security analyst must choose the best performance Security Analysis Static Tool(SAST)in terms of discovering the greatest number of security vulnerabilities as possible.To compare static analysis tools for web applications,an adapted benchmark to the vulnerability categories included in the known standard Open Web Application Security Project(OWASP)Top Ten project is required.The information of the security effectiveness of a commercial static analysis tool is not usually a publicly accessible research and the state of the art on static security tool analyzers shows that the different design and implementation of those tools has different effectiveness rates in terms of security performance.Given the significant cost of commercial tools,this paper studies the performance of seven static tools using a new methodology proposal and a new benchmark designed for vulnerability categories included in the known standard OWASP Top Ten project.Thus,the practitioners will have more precise information to select the best tool using a benchmark adapted to the last versions of OWASP Top Ten project.The results of this work have been obtaining using widely acceptable metrics to classify them according to three different degree of web application criticality.

A Two-folded Impact Analysis of Schema Changes on Database Applications

Database applications are becoming increasingly popular, mainly due to the advanced data management facilities that the underlying database management system offers compared against traditional legacy software applications. The interaction, however, of such applications with the database system introduces a number of issues, among which, this paper addresses the impact analysis of the changes performed at the database schema level. Our motivation is to provide the software engineers of database applications with automated methods that facilitate major maintenance tasks, such as source code corrections and regression testing, which should be triggered by the occurrence of such changes. The presented impact analysis is thus two-folded： the impact is analysed in terms of both the affected source code statements and the affected test suites concerning the testing of these applications. To achieve the former objective, a program slicing technique is employed, which is based on an extended version of the program dependency graph. The latter objective requires the analysis of test suites generated for database applications, which is accomplished by employing testing techniques tailored for this type of applications. Utilising both the slicing and the testing techniques enhances program comprehension of database applications, while also supporting the development of a number of practical metrics regarding their maintainability against schema changes. To evaluate the feasibility and effectiveness of the presented techniques and metrics, a software tool, called DATA, has been implemented. The experimental results from its usage on the TPC-C case study are reported and analysed.

Combinatorial Method with Static Analysis for Source Code Security in Web Applications

Security weaknesses in web applications deployed in cloud architectures can seriously affect its data confidentiality and integrity.The construction of the procedure utilized in the static analysis tools of source code security differs and therefore each tool finds a different number of each weakness type for which it is designed.To utilize the possible synergies different static analysis tools may process,this work uses a new method to combine several source codes aiming to investigate how to increase the performance of security weakness detection while reducing the number of false positives.Specifically,five static analysis tools will be combined with the designed method to study their behavior using an updated benchmark for OWASP Top Ten Security Weaknesses(OWASP TTSW).The method selects specific metrics to rank the tools for different criticality levels of web applications considering different weights in the ratios.The findings show that simply including more tools in a combination is not synonymous with better results;it depends on the specific tools included in the combination due to their different designs and techniques.

APPLICATION OF MONTE-CARLO SIMULATED ANNEALING ON CONFORMATIONAL ANALYSIS

Monte-Carlo simulated annealing method (SA) was applied to the conformational analysis of flexible molecules such as peptides. The programs SAMM and SAPEP have been developed, using MM2 force field for organic molecules and ECEPP/2 force field for peptides respectively.

Sentiment Analysis with Tweets Behaviour in Twitter Streaming API

Twitter is a radiant platform with a quick and effective technique to analyze users’perceptions of activities on social media.Many researchers and industry experts show their attention to Twitter sentiment analysis to recognize the stakeholder group.The sentiment analysis needs an advanced level of approaches including adoption to encompass data sentiment analysis and various machine learning tools.An assessment of sentiment analysis in multiple fields that affect their elevations among the people in real-time by using Naive Bayes and Support Vector Machine(SVM).This paper focused on analysing the distinguished sentiment techniques in tweets behaviour datasets for various spheres such as healthcare,behaviour estimation,etc.In addition,the results in this work explore and validate the statistical machine learning classifiers that provide the accuracy percentages attained in terms of positive,negative and neutral tweets.In this work,we obligated Twitter Application Programming Interface(API)account and programmed in python for sentiment analysis approach for the computational measure of user’s perceptions that extract a massive number of tweets and provide market value to the Twitter account proprietor.To distinguish the results in terms of the performance evaluation,an error analysis investigates the features of various stakeholders comprising social media analytics researchers,Natural Language Processing(NLP)developers,engineering managers and experts involved to have a decision-making approach.

APPLICATION OF THERMAL ANALYSIS TO THE QUICK DETERMINATION OF THE PROXIMATE ANALYSIS ITEMS AND CALORIC VALUE FOR COAL

A research on the application of a TG-DTA(thermal gravimetry aifferential thermal analysis)to the quick determination of moisture,organic volatile fixed carbon,ash and caloric value for coal is described.

APPLICATION OF HARMONIC ANALYSIS METHOD TO RESEARCH ON ROTOR AIRLOADS

According to the rotor vortex theory,the rotor circulation and the rotorinduced velocity are developed into Fourier series.The circulation distributionalong blade spanwise is expressed in terms of segment-by-segment linearfunctions.In consequence the induced velocity equations and the circulationequations are derived.The engineering application of the rotor vortex theoryis provided.Then the induced velocity and its harmonic components are obtainedto provide a quantitative basis for the vortex model.For calculatingeach harmonic component of the induced velocity a simplified method is putforward which considers the effects of each order circulation with neglectingthose of higher order.The method saves the computer time and is of significantbenefit.

APPLICATION OF WAXD AND SAXS TO STRUCTURAL ANALYSIS OF PA1010+BMI

WAXD and SAXS for PA1010+BMI system have been measured. The results obtained with the help of Ruland and ID EDCF. showed that Wc1x1, the long period L and the thickness of the crystal lamellae do decreased with BMI content at PA10101+BMI system. Comparing both experimental and calculated densitl c values indicated thst the values were of better conformity. Adding BHI into PA1010 caused an increase in distortion.The results from SAXS analysis have also led to the conclusion that a crystal-amorphous interphase could exist in lamellae of Semicrystalline polymers and a three-phase model instead of traditional two-phase model should be used.

THE ELASTO-PLASTIC STRAIN ANALYSIS OF NOTCHED PLATE UNDER CYCLIC LOADING—AN APPLICATION OF PLASTIC ANISOTROPIC HARDENING THEORY

In order to predict the life of engineering structures, it is necessary to investigate the strain distribution in notched members. In gineral, the Uauschinger Effect of materials under cyclic loading is not negligible, and so the anisolropic hardening model has been suggested. From the comparison between the calculated and experimental results in this paper, we can see that even the linear kinematic hardening model is quite suitable for strain analysis under cyclic loading.

APPLICATION OF REMOTE SENSING IN THE ANALYSIS OF WATER ENVIRONMENT IN ZHUJIANG RIVER ESTUARY

1. PREFACE Lingdingyang is a trumpet estuary. It accepts the runoff of the Dongjiang River, the Beijiang River, the Zhengjiang River and the Liusihe River. It also accepts a part of the runoff of the Xijiang River. Its mean year runoff is 1.742×10" M^3. In resent ten years, industry and agriculture are developing rapidly in Guangzhou City, Dongguan City, Zhongshan City, Shunde County, Panyu County. Lingdingyang’s pollution is increesing. Water quality of lingdingyang is steadily deteriorated. In order to investigate the situation of water environment of Lingdingyang, we study its static environmental capacity of nitrogen and phosphorus. LANDSAT imageries are used in the study. The concentrations of nitrogen and phosphorous is detected by convention method.

APPLICATIONS OF REMOTE SENSING IN ENVIRONMENTAL INVESTIGATION AND CHANGE ANALYSIS IN THE THREE GORGES AREA

1. INTRODUCTION The proposed Three Gorges Project, one of the biggest hydroelectric projects in the world, will dam the middle reaches of the Changjiang (Yangtze) River, the third longest river in the world, and form a large reservoir. Its impacts on environment have attracted wide attention. Entrusted by National Scientific-Technical Commission, the Chinese Academy of Sciences (CAS) was in charge of a research project on this issuse from 1984 to 1989. Tho use of remote sensing played an important role in the project considering the study area is mountainous and not convenientlv located, which makes it difficult to conduct the research onlv using conventional means.