Deep-learning-based methods for super-resolution fluorescence microscopy

The algorithm used for reconstruction or resolution enhancement is one of the factors affectingthe quality of super-resolution images obtained by fluorescence microscopy.Deep-learning-basedalgorithms have achieved stateof-the-art performance in super-resolution fluorescence micros-copy and are becoming increasingly attractive.We firstly introduce commonly-used deep learningmodels,and then review the latest applications in terms of the net work architectures,the trainingdata and the loss functions.Additionally,we discuss the challenges and limits when using deeplearning to analyze the fluorescence microscopic data,and suggest ways to improve the reliability and robustness of deep learning applications.

Physics-Based Active Learning for Design Space Exploration and Surrogate Construction for Multiparametric Optimization

The sampling of the training data is a bottleneck in the development of artificial intelligence(AI)models due to the processing of huge amounts of data or to the difficulty of access to the data in industrial practices.Active learning(AL)approaches are useful in such a context since they maximize the performance of the trained model while minimizing the number of training samples.Such smart sampling methodologies iteratively sample the points that should be labeled and added to the training set based on their informativeness and pertinence.To judge the relevance of a data instance,query rules are defined.In this paper,we propose an AL methodology based on a physics-based query rule.Given some industrial objectives from the physical process where the AI model is implied in,the physics-based AL approach iteratively converges to the data instances fulfilling those objectives while sampling training points.Therefore,the trained surrogate model is accurate where the potentially interesting data instances from the industrial point of view are,while coarse everywhere else where the data instances are of no interest in the industrial context studied.

Faster split-based feedback network for image super-resolution

Although most of the existing image super-resolution(SR)methods have achieved superior performance,contrastive learning for high-level tasks has not been fully utilized in the existing image SR methods based on deep learning.This work focuses on two well-known strategies developed for lightweight and robust SR,i.e.,contrastive learning and feedback mechanism,and proposes an integrated solution called a split-based feedback network(SPFBN).The proposed SPFBN is based on a feedback mechanism to learn abstract representations and uses contrastive learning to explore high information in the representation space.Specifically,this work first uses hidden states and constraints in recurrent neural network(RNN)to implement a feedback mechanism.Then,use contrastive learning to perform representation learning to obtain high-level information by pushing the final image to the intermediate images and pulling the final SR image to the high-resolution image.Besides,a split-based feedback block(SPFB)is proposed to reduce model redundancy,which tolerates features with similar patterns but requires fewer parameters.Extensive experimental results demonstrate the superiority of the proposed method in comparison with the state-of-the-art methods.Moreover,this work extends the experiment to prove the effectiveness of this method and shows better overall reconstruction quality.

Effects of Health Education with Problem-Based Learning Approaches on the Knowledge, Attitude, Practice and Coping Skills of Women with High-Risk Pregnancies in Plateau Areas

Objective: Given the unique cultural background, way of life, and physical environment of the Tibetan Plateau, this study aims to investigate the effects of health education using problem-based learning (PBL) approaches on the knowledge, attitude, practice, and coping skills of women with high-risk pregnancies in this region. Methods: 76 high-risk pregnancy cases were enrolled at Tibet’s Linzhi People’s Hospital between September 2023 and April 2024. 30 patients admitted between September 2023 and December 2023 were selected as the control group and were performed with regular patient education. 46 patients admitted between January 2024 and April 2024 were selected as the observation group and were performed regular patient education with problem-based learning approaches. Two groups’ performance on their health knowledge, attitude, practice and coping skills before and after interventions were evaluated, and patient satisfaction were measured at the end of the study. Results: There was no statistical significance (P P P Conclusions: Health education with problem-based learning approaches is worth promoting as it can help high-risk pregnant women in plateau areas develop better health knowledge, attitude and practice and healthier coping skills. Also, it can improve patient sanctification.

Prediction of Damping Capacity Demand in Seismic Base Isolators via Machine Learning

Base isolators used in buildings provide both a good acceleration reduction and structural vibration control structures.The base isolators may lose their damping capacity over time due to environmental or dynamic effects.This deterioration of them requires the determination of the maintenance and repair needs and is important for the long-termisolator life.In this study,an artificial intelligence prediction model has been developed to determine the damage and maintenance-repair requirements of isolators as a result of environmental effects and dynamic factors over time.With the developed model,the required damping capacity of the isolator structure was estimated and compared with the previously placed isolator capacity,and the decrease in the damping property was tried to be determined.For this purpose,a data set was created by collecting the behavior of structures with single degrees of freedom(SDOF),different stiffness,damping ratio and natural period isolated from the foundation under far fault earthquakes.The data is divided into 5 different damping classes varying between 10%and 50%.Machine learning model was trained in damping classes with the data on the structure’s response to random seismic vibrations.As a result of the isolator behavior under randomly selected earthquakes,the recorded motion and structural acceleration of the structure against any seismic vibration were examined,and the decrease in the damping capacity was estimated on a class basis.The performance loss of the isolators,which are separated according to their damping properties,has been tried to be determined,and the reductions in the amounts to be taken into account have been determined by class.In the developed prediction model,using various supervised machine learning classification algorithms,the classification algorithm providing the highest precision for the model has been decided.When the results are examined,it has been determined that the damping of the isolator structure with the machine learning method is predicted successfully at a level exceeding 96%,and it is an effective method in deciding whether there is a decrease in the damping capacity.

Fast image super-resolution algorithm based on multi-resolution dictionary learning and sparse representation

Sparse representation has attracted extensive attention and performed well on image super-resolution（SR） in the last decade. However, many current image SR methods face the contradiction of detail recovery and artifact suppression. We propose a multi-resolution dictionary learning（MRDL） model to solve this contradiction, and give a fast single image SR method based on the MRDL model. To obtain the MRDL model, we first extract multi-scale patches by using our proposed adaptive patch partition method（APPM）. The APPM divides images into patches of different sizes according to their detail richness. Then, the multiresolution dictionary pairs, which contain structural primitives of various resolutions, can be trained from these multi-scale patches.Owing to the MRDL strategy, our SR algorithm not only recovers details well, with less jag and noise, but also significantly improves the computational efficiency. Experimental results validate that our algorithm performs better than other SR methods in evaluation metrics and visual perception.

A brief survey on deep learning based image super-resolution

Image super-resolution(SR)is an important technique for improving the resolution and quality of images.With the great progress of deep learning,image super-resolution achieves remarkable improvements recently.In this work,a brief survey on recent advances of deep learning based single image super-resolution methods is systematically described.The existing studies of SR techniques are roughly grouped into ten major categories.Besides,some other important issues are also introduced,such as publicly available benchmark datasets and performance evaluation metrics.Finally,this survey is concluded by highlighting four future trends.

Hyperspectral Image Super-Resolution Meets Deep Learning:A Survey and Perspective

Hyperspectral image super-resolution,which refers to reconstructing the high-resolution hyperspectral image from the input low-resolution observation,aims to improve the spatial resolution of the hyperspectral image,which is beneficial for subsequent applications.The development of deep learning has promoted significant progress in hyperspectral image super-resolution,and the powerful expression capabilities of deep neural networks make the predicted results more reliable.Recently,several latest deep learning technologies have made the hyperspectral image super-resolution method explode.However,a comprehensive review and analysis of the latest deep learning methods from the hyperspectral image super-resolution perspective is absent.To this end,in this survey,we first introduce the concept of hyperspectral image super-resolution and classify the methods from the perspectives with or without auxiliary information.Then,we review the learning-based methods in three categories,including single hyperspectral image super-resolution,panchromatic-based hyperspectral image super-resolution,and multispectral-based hyperspectral image super-resolution.Subsequently,we summarize the commonly used hyperspectral dataset,and the evaluations for some representative methods in three categories are performed qualitatively and quantitatively.Moreover,we briefly introduce several typical applications of hyperspectral image super-resolution,including ground object classification,urban change detection,and ecosystem monitoring.Finally,we provide the conclusion and challenges in existing learning-based methods,looking forward to potential future research directions.

Contrastive Learning for Blind Super-Resolution via A Distortion-Specific Network

Previous deep learning-based super-resolution(SR)methods rely on the assumption that the degradation process is predefined(e.g.,bicubic downsampling).Thus,their performance would suffer from deterioration if the real degradation is not consistent with the assumption.To deal with real-world scenarios,existing blind SR methods are committed to estimating both the degradation and the super-resolved image with an extra loss or iterative scheme.However,degradation estimation that requires more computation would result in limited SR performance due to the accumulated estimation errors.In this paper,we propose a contrastive regularization built upon contrastive learning to exploit both the information of blurry images and clear images as negative and positive samples,respectively.Contrastive regularization ensures that the restored image is pulled closer to the clear image and pushed far away from the blurry image in the representation space.Furthermore,instead of estimating the degradation,we extract global statistical prior information to capture the character of the distortion.Considering the coupling between the degradation and the low-resolution image,we embed the global prior into the distortion-specific SR network to make our method adaptive to the changes of distortions.We term our distortion-specific network with contrastive regularization as CRDNet.The extensive experiments on synthetic and realworld scenes demonstrate that our lightweight CRDNet surpasses state-of-the-art blind super-resolution approaches.

Triplet Label Based Image Retrieval Using Deep Learning in Large Database

Recent days,Image retrieval has become a tedious process as the image database has grown very larger.The introduction of Machine Learning(ML)and Deep Learning(DL)made this process more comfortable.In these,the pair-wise label similarity is used tofind the matching images from the database.But this method lacks of limited propose code and weak execution of misclassified images.In order to get-rid of the above problem,a novel triplet based label that incorporates context-spatial similarity measure is proposed.A Point Attention Based Triplet Network(PABTN)is introduced to study propose code that gives maximum discriminative ability.To improve the performance of ranking,a corre-lating resolutions for the classification,triplet labels based onfindings,a spatial-attention mechanism and Region Of Interest(ROI)and small trial information loss containing a new triplet cross-entropy loss are used.From the experimental results,it is shown that the proposed technique exhibits better results in terms of mean Reciprocal Rank(mRR)and mean Average Precision(mAP)in the CIFAR-10 and NUS-WIPE datasets.

Aspect based sentiment analysis using multi-criteria decision-making and deep learning under COVID-19 pandemic in India

The COVID-19 pandemic has a significant impact on the global economy and health.While the pandemic continues to cause casualties in millions,many countries have gone under lockdown.During this period,people have to stay within walls and become more addicted towards social networks.They express their emotions and sympathy via these online platforms.Thus,popular social media(Twitter and Facebook)have become rich sources of information for Opinion Mining and Sentiment Analysis on COVID-19-related issues.We have used Aspect Based Sentiment Analysis to anticipate the polarity of public opinion underlying different aspects from Twitter during lockdown and stepwise unlock phases.The goal of this study is to find the feelings of Indians about the lockdown initiative taken by the Government of India to stop the spread of Coronavirus.India-specific COVID-19 tweets have been annotated,for analysing the sentiment of common public.To classify the Twitter data set a deep learning model has been proposed which has achieved accuracies of 82.35%for Lockdown and 83.33%for Unlock data set.The suggested method outperforms many of the contemporary approaches(long shortterm memory,Bi-directional long short-term memory,Gated Recurrent Unit etc.).This study highlights the public sentiment on lockdown and stepwise unlocks,imposed by the Indian Government on various aspects during the Corona outburst.

Ensemble Based Learning with Accurate Motion Contrast Detection

Recent developments in computer vision applications have enabled detection of significant visual objects in video streams.Studies quoted in literature have detected objects from video streams using Spatiotemporal Particle Swarm Optimization(SPSOM)and Incremental Deep Convolution Neural Networks(IDCNN)for detecting multiple objects.However,the study considered opticalflows resulting in assessing motion contrasts.Existing methods have issue with accuracy and error rates in motion contrast detection.Hence,the overall object detection performance is reduced significantly.Thus,consideration of object motions in videos efficiently is a critical issue to be solved.To overcome the above mentioned problems,this research work proposes a method involving ensemble approaches to and detect objects efficiently from video streams.This work uses a system modeled on swarm optimization and ensemble learning called Spatiotemporal Glowworm Swarm Optimization Model(SGSOM)for detecting multiple significant objects.A steady quality in motion contrasts is maintained in this work by using Chebyshev distance matrix.The proposed system achieves global optimization in its multiple object detection by exploiting spatial/temporal cues and local constraints.Its experimental results show that the proposed system scores 4.8%in Mean Absolute Error(MAE)while achieving 86%in accuracy,81.5%in precision,85%in recall and 81.6%in F-measure and thus proving its utility in detecting multiple objects.

A hybrid agent⁃based machine learning method for human⁃centred energy consumption prediction

Occupant behaviour has significant impacts on the performance of machine learning algorithms when predicting building energy consumption.Due to a variety of reasons(e.g.,underperforming building energy management systems or restrictions due to privacy policies),the availability of occupational data has long been an obstacle that hinders the performance of machine learning algorithms in predicting building energy consumption.Therefore,this study proposed an agent⁃based machine learning model whereby agent⁃based modelling was employed to generate simulated occupational data as input features for machine learning algorithms for building energy consumption prediction.Boruta feature selection was also introduced in this study to select all relevant features.The results indicated that the performances of machine learning algorithms in predicting building energy consumption were significantly improved when using simulated occupational data,with even greater improvements after conducting Boruta feature selection.

Exploring the Application Effect of Flipped Classroom Combined with Problem-Based Learning Teaching Method in Clinical Skills Teaching of Standardized Training for Resident Doctors of Traditional Chinese Medicine

Objective: To explore the application effect of flipped classroom combined with problem-based learning teaching method in clinical skills teaching of standardized training for resident doctors of traditional Chinese Medicine. Methods: The study used the experimental control method. The study lasted from September to November 2022. The subjects of this study were 49 students of standardized training for resident doctors of traditional Chinese Medicine from grades 2020, 2021 and 2022 of Dazhou integrated TCM & Western Medicine Hospital. They were randomly divided into experiment group (25) and control group (24). The experiment group adopted flipped classroom combined with problem-based learning teaching method, and the control group adopted traditional teaching method. The teaching content was 4 basic clinical skill projects, including four diagnoses of traditional Chinese Medicine, cardiopulmonary resuscitation, dressing change procedure, acupuncture and massage. The evaluation method was carried out by comparing the students’ performance and a self-designed questionnaire was used to investigate the students’ evaluation of the teaching method. Results: The test scores of total scores in the experimental group (90.12 ± 5.89) were all higher than those in the control group (81.47 ± 7.96) (t = 4.53, P P Conclusions: The teaching process of the flipped classroom combined with problem-based learning teaching method is conducive to improving the efficiency of classroom teaching, cultivating students’ self-learning ability, and enhancing students’ willingness to learn.

A Research of the Course “Taishan Cultural Communication with the World” under Blended Learning Model and Outcome-Based Education Concept

The course “Taishan Cultural Communication with the World” has been online and offline teaching and learning for two terms based on the theoretical ideas: Blended Learning and Outcome-Based Education. This paper uses the data from one semester to state how to carry out the program and the good results. At the same time disadvantages are also the points that should be taken into consideration. From the teaching and learning practice, students have benefited from the online videos, complementary materials and discussions;they need to be guided as well, especially the guidance offline to make up. Furthermore, the balance of time online and offline is a great challenge.

TBL(Team-based learning)教学法在局解教学中的设计与评价

为适应现代医学发展的要求,在日益增多的医学教学改革尝试中,TBL教学法引起人们的关注。本文通过在局部解剖学教学中开展TBL教学,并且和传统教学方法做了对比研究。结果提示在局部解剖学教学中采用TBL教学法有利于提高学生学习兴趣及解决问题的能力,有利于动态评价学生的学习状态。

PBL(Problem-based Learning)教学法道路规划与几何设计教学中的应用与探索

立足于道路规划与几何设计信息量大,涉及专业基础知识广、实践性强等特点,将PBL教学法在教学中进行了应用与探索,从教师设计问题、组建学习小组、问题探索与交流、教师总结评价等方面进行了教学设计和应用研究,实践证明PBL教学有助于提高学生的自主创新学习能力及学习的积极性,显著提升了教学效果。

PSMFNet:Lightweight Partial Separation and Multiscale Fusion Network for Image Super-Resolution

The employment of deep convolutional neural networks has recently contributed to significant progress in single image super-resolution(SISR)research.However,the high computational demands of most SR techniques hinder their applicability to edge devices,despite their satisfactory reconstruction performance.These methods commonly use standard convolutions,which increase the convolutional operation cost of the model.In this paper,a lightweight Partial Separation and Multiscale Fusion Network(PSMFNet)is proposed to alleviate this problem.Specifically,this paper introduces partial convolution(PConv),which reduces the redundant convolution operations throughout the model by separating some of the features of an image while retaining features useful for image reconstruction.Additionally,it is worth noting that the existing methods have not fully utilized the rich feature information,leading to information loss,which reduces the ability to learn feature representations.Inspired by self-attention,this paper develops a multiscale feature fusion block(MFFB),which can better utilize the non-local features of an image.MFFB can learn long-range dependencies from the spatial dimension and extract features from the channel dimension,thereby obtaining more comprehensive and rich feature information.As the role of the MFFB is to capture rich global features,this paper further introduces an efficient inverted residual block(EIRB)to supplement the local feature extraction ability of PSMFNet.A comprehensive analysis of the experimental results shows that PSMFNet maintains a better performance with fewer parameters than the state-of-the-art models.

Pyramid Separable Channel Attention Network for Single Image Super-Resolution

Single Image Super-Resolution(SISR)technology aims to reconstruct a clear,high-resolution image with more information from an input low-resolution image that is blurry and contains less information.This technology has significant research value and is widely used in fields such as medical imaging,satellite image processing,and security surveillance.Despite significant progress in existing research,challenges remain in reconstructing clear and complex texture details,with issues such as edge blurring and artifacts still present.The visual perception effect still needs further enhancement.Therefore,this study proposes a Pyramid Separable Channel Attention Network(PSCAN)for the SISR task.Thismethod designs a convolutional backbone network composed of Pyramid Separable Channel Attention blocks to effectively extract and fuse multi-scale features.This expands the model’s receptive field,reduces resolution loss,and enhances the model’s ability to reconstruct texture details.Additionally,an innovative artifact loss function is designed to better distinguish between artifacts and real edge details,reducing artifacts in the reconstructed images.We conducted comprehensive ablation and comparative experiments on the Arabidopsis root image dataset and several public datasets.The experimental results show that the proposed PSCAN method achieves the best-known performance in both subjective visual effects and objective evaluation metrics,with improvements of 0.84 in Peak Signal-to-Noise Ratio(PSNR)and 0.017 in Structural Similarity Index(SSIM).This demonstrates that the method can effectively preserve high-frequency texture details,reduce artifacts,and have good generalization performance.

Multi-prior physics-enhanced neural network enables pixel super-resolution and twin-imagefree phase retrieval from single-shot hologram

Digital in-line holographic microscopy(DIHM)is a widely used interference technique for real-time reconstruction of living cells’morphological information with large space-bandwidth product and compact setup.However,the need for a larger pixel size of detector to improve imaging photosensitivity,field-of-view,and signal-to-noise ratio often leads to the loss of sub-pixel information and limited pixel resolution.Additionally,the twin-image appearing in the reconstruction severely degrades the quality of the reconstructed image.The deep learning(DL)approach has emerged as a powerful tool for phase retrieval in DIHM,effectively addressing these challenges.However,most DL-based strategies are datadriven or end-to-end net approaches,suffering from excessive data dependency and limited generalization ability.Herein,a novel multi-prior physics-enhanced neural network with pixel super-resolution(MPPN-PSR)for phase retrieval of DIHM is proposed.It encapsulates the physical model prior,sparsity prior and deep image prior in an untrained deep neural network.The effectiveness and feasibility of MPPN-PSR are demonstrated by comparing it with other traditional and learning-based phase retrieval methods.With the capabilities of pixel super-resolution,twin-image elimination and high-throughput jointly from a single-shot intensity measurement,the proposed DIHM approach is expected to be widely adopted in biomedical workflow and industrial measurement.