Based on transformation acoustic methodology, we propose an algorithm for designing acoustic non-resonant lens antenna, which is competent to generate multiple directive beams that are pointing at the desired directio...Based on transformation acoustic methodology, we propose an algorithm for designing acoustic non-resonant lens antenna, which is competent to generate multiple directive beams that are pointing at the desired direction.Unattainable with previous works, the present approach is capable of adjusting the directivity of each radiated beam individually, which is of the utmost importance in several acoustic applications such as in sonar systems. A linear transformation function is intentionally used for eliminating the inhomogeneity of the obtained materials and to pave the way towards more general acoustic patterns. Several numerical simulations are performed to show the capability of the proposed method in manipulating the acoustic waves. To authenticate the concept,a structure that can generate four beams with different directivities is realized with non-resonant meta-fluid bi-layered structure through effective medium theory.展开更多
AIM： To study the effect of discoidin I-like domaincontaining protein 3（EDIL3） depletion on the proliferation and epithelial-mesenchymal transition（EMT） in human lens epithelial cells（LECs）. METHODS： RNA inter...AIM： To study the effect of discoidin I-like domaincontaining protein 3（EDIL3） depletion on the proliferation and epithelial-mesenchymal transition（EMT） in human lens epithelial cells（LECs）. METHODS： RNA interference was used to inhibit the expression of EDIL3 in human LECs in vitro. The morphology of cells was observed using an inverted microscope. Cell proliferation was assessed using Ed U kit. Cell migration was investigated using Transwell chamber and EMT of LECs was assessed using confocal microscope and Western blotting. The transforming growth factor β（TGFβ） pathway was investigated using Western blotting. RESULTS： The data showed that silencing EDIL3 expression changed LECs morphology and suppressed LECs proliferation（P〈0.05） and migration（P〈0.01）. Furthermore, the result of Western blotting showed that EDIL3 depletion reduced the expression of α-smooth muscle actin（α-SMA）（P〈0.001） and vimentin（P〈0.01）, while increased the expression of E-cadherin（P〈0.001）. EDIL3 depletion could suppress the phosphorylation of Smad2（P〈0.01） and Smad3（P〈0.01） and the activation of exracellular signal regulated kinase（ERK）（P〈0.05）. CONCLUSION： The findings indicate that EDIL3 might participate in the proliferation and EMT in LECs via TGFβ pathway and may be a potential therapeutic target for the treatment of posterior capsule opacification.展开更多
文摘Based on transformation acoustic methodology, we propose an algorithm for designing acoustic non-resonant lens antenna, which is competent to generate multiple directive beams that are pointing at the desired direction.Unattainable with previous works, the present approach is capable of adjusting the directivity of each radiated beam individually, which is of the utmost importance in several acoustic applications such as in sonar systems. A linear transformation function is intentionally used for eliminating the inhomogeneity of the obtained materials and to pave the way towards more general acoustic patterns. Several numerical simulations are performed to show the capability of the proposed method in manipulating the acoustic waves. To authenticate the concept,a structure that can generate four beams with different directivities is realized with non-resonant meta-fluid bi-layered structure through effective medium theory.
基金Supported by the National Natural Science Foundation of China (No.81700839)Military logistics scientific research project (No.BWS12J030)+2 种基金Natural Science Foundation of Shanghai (No.15ZR1413200)Research Foundation for Youth of Second Military Medical University (No.2016QN13)Research Foundation for Youth of Changhai Hospital (No.CH201712)
文摘AIM： To study the effect of discoidin I-like domaincontaining protein 3（EDIL3） depletion on the proliferation and epithelial-mesenchymal transition（EMT） in human lens epithelial cells（LECs）. METHODS： RNA interference was used to inhibit the expression of EDIL3 in human LECs in vitro. The morphology of cells was observed using an inverted microscope. Cell proliferation was assessed using Ed U kit. Cell migration was investigated using Transwell chamber and EMT of LECs was assessed using confocal microscope and Western blotting. The transforming growth factor β（TGFβ） pathway was investigated using Western blotting. RESULTS： The data showed that silencing EDIL3 expression changed LECs morphology and suppressed LECs proliferation（P〈0.05） and migration（P〈0.01）. Furthermore, the result of Western blotting showed that EDIL3 depletion reduced the expression of α-smooth muscle actin（α-SMA）（P〈0.001） and vimentin（P〈0.01）, while increased the expression of E-cadherin（P〈0.001）. EDIL3 depletion could suppress the phosphorylation of Smad2（P〈0.01） and Smad3（P〈0.01） and the activation of exracellular signal regulated kinase（ERK）（P〈0.05）. CONCLUSION： The findings indicate that EDIL3 might participate in the proliferation and EMT in LECs via TGFβ pathway and may be a potential therapeutic target for the treatment of posterior capsule opacification.
