Construction and selection of the natural immune Fab antibody phage display library from patients with colorectal cancer

AIM: To construct the natural immune Fab antibody phage display libraries of colorectal cancer and to select antibodies related with colorectal cancer. METHODS: Extract total RNA from tissue of local cancer metastasis lymph nodes of patients with colorectal cancer. RT-PCR was used to amplify the heavy chain Fd and light chain kappa and the amplification products were inserted successively into the vector pComb3 to construct the human libraries of Fab antibodies. They were then panned by phage display technology. By means of Dot immunoblotting and ELISA, the libraries were identified and the Fab phage antibodies binding with antigens of colorectal cancer were selected. RESULTS: The amplified fragments of Fd and kappa gained by RT-PCR were about 650 bp. Fd and kappa PCR products were subsequently inserted into the vector pComb3, resulting in a recombination rate of 40% and the volume of Fab phage display library reached 1.48 x 10(6).The libraries were enriched about 120-fold by 3 cycles of adsorption-elution-multiplication (panning). Dot immunoblotting showed Fab expressions on the phage libraries and ELISA showed 5 clones of Fab phage antibodies which had binding activities with antigens of colorectal cancer. CONCLUSION: The natural immune Fab antibody phage display libraries of colorectal cancer were constructed. They could be used to select the relative antibodies of colorectal cancer.

Diversity of Microflora in Colonic Mucus from Severe Ulcerative Colitis Patients Analyzed by Terminal Restriction Fragment Length Polymorphism and Clone Libraries of Bacterial 16S rRNA Gene Sequences

Although the gut microflora is thought to be an essential factor in the development of ulcerative colitis (UC), the entire gut microflora occurring in UC remains unknown. Most studies use feces to represent the microflora distribution;however, here we analyzed the bacterial diversity in colonic mucus from UC patients receiving colectomy surgery and control patients. The diversity of microflora was investigated using a combination of terminal restriction fragment length polymorphism (T-RFLP) and clone library analyses of the 16S rRNA gene sequences. In the T-RFLP analysis, the number of terminal restriction fragments (T-RFs) decreased significantly in UC patients when compared to control samples. Also in the clone library analysis, the number of operational taxonomic units (OTU) and the Shannon diversity index were reduced significantly in UC patients. These molecular analyses reveal an overall dysbiosis in UC patients. No specific pathogen was found, and a strong negative correlation in relative abundance of bacterial populations was observed between the phyla Bacteroidetes and Firmicutes in the UC patients. This is the first report showing a significant correlation between these two phyla, which may be important characteristics in the pathogenesis of UC.

Management Communication in University’s Digital Library

Digital library is a process of exploiting more scientific methods to construct a social knowledge network. It represents an “extension” from a traditional library. The core and essence is to build a favorable surroundings for knowledge accumulation, knowledge transfer and knowledge communication. Management communication covers information transfer and organization cooperation. Compared with traditional library, the management communication in digital library shoulders the commitment to support knowledge innovation. On the basis of analysis of the connotation of digital library, this paper analysed the aspects of management communication, including communicators, communication styles, contents, channels and outer communication, all of which tries to construct an integrated model of management communication in a digital library and turns a library into an actual “knowledge fountain”.

Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers

Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oli- gomers and constructed a have human Fab phage display library based on blood samples from six healthy people. After three rounds of biopanning in vitro, a human single-domain antibody that spe- cifically recognized amyloid-beta 42 oligomers was identified. Western blot and enzyme-linked im- munosorbent assay demonstrated this antibody bound specifically to human amyloid-beta 42 tetramer and nonamer, but not the monomer or high molecular weight oligomers. This study suc- cessfully constructed a human phage display library and screened a single-domain antibody that specifically recognized amyloid-beta 42 oligomers.

The Construction of a New Translation Ethics from the Perspective of Chinese Philosophy

As a cross-linguistic,cross-cultural,and cross-social activity,translation is affected by cultural moralities.Since the ethical standard of faithfulness has been abandoned by theories of cultural criticism and the power of translators’manipulation has been constantly exaggerated,the study of translation ethics is an effective remedy for a current dilemma in translation studies.It is a common phenomenon for some translators to erase the linguistic and cultural differences of Chinese literature by catering to target readers’reception.Social ethics is the foundation of a nation’s translation ethics,which means that thoughts from the Western world may not be suitable for the current situation in China.Learning from concepts of Chinese philosophy including harmony in diversity,seeking common ground while reserving differences,and honesty to formulate a new translation ethics will surely restrain translators’subjectivity within appropriate limits and develop world culture in more harmonious fashion.

Let's 3G: ZTE's Future-Oriented 3G Philosophy

ZTE Corporation is China's largest listed telecommunication equipment manufacturer and a total solution provider. In the past 20 years ZTE has achieved a very rapid growth while in the past 3 years it attained an average annual growth rate of over 35%. In order to attain a continual growth in the coming years, ZTE is now strengthening up its globalization strategy. As a future-oriented technology, 3G has become the focus. Based on a brief introduction of 3G applications at home and abroad, this paper puts forward ZTE' s 3G development strategy.

Conceptions of Intuition in Poincar6＇s Philosophy of Mathematics

The aim of this paper is to contribute to the identification and characterization of the various types of intuition put forward by Poincar6, taking his texts as a laboratory for looking for what intuition might be. I will stress that these diverse conceptions are mainly formulated in the context of Poincar6＇s controversies in opposition to logicism, to formalism, and in the context of Poincar6＇s very peculiar conventionalism. I will try to demonstrate that, in each case, Poincar~ comes close to a specific tradition （Kant, of course, but also Leibniz and Peirce）.

A Test Items Knowledge Library System for Adaptive Learning

A test items knowledge library system of for adaptive learning is proposed in this paper. The first step is to carry out the quantity and quality analysis of the test items by using the Bloom＇s revised taxonomy and scale anchoring respectively to produce the characteristics for test items. A smoothing method of arbitrary anchoring revised from scale anchoring is first proposed to make tests more accurate in distinguishing test levels. In addition, raised three dimensional indicators based on the Bloom＇s revised taxonomy are adopted to validate test contents and therefore it concretely describes the examining function of items. The items obtained have the precise and concrete properties; an item knowledge library is therefore constructed combining teaching materials and items using the technologies of ontology and knowledge management. Finally, a knowledge library system of test items is established to achieve the purpose of adaptive learning for learners.

A Review on the Evolution and Development of Searle＇ s Philosophy of Mind

The evolution and development of Searle’s philosophy of mind can be divided into two important stages,i.e.the origin and beginning stage between 1960sand 1970s,the formation and development stage since 1980s.Searle’s Speech Act Theory started the research on philosophy of mind,which is considered as the origin of his philosophy of mind.The Theories of Intentionality and Chinese Room Argument are the most important and most influential academic findings in his research in the field of philosophy of mind,which lay a solid theoretical foundation for the formation and development of his theories of philosophy of mind.Searle’s philosophy of mind finds wide prospect in the research field,which provides a new direction to understand the world and probe into mind,proposes a new perspective and methodology for the study of language,and promotes the new development of the study of language.

The Physical Laws and Mathematical Axioms of the Brain’s OS and the Traditional Fundamental Laws of Thought of Logic and Philosophy

This article presents four (4) additions to a book on the brain’s OS published by SciRP in 2015 [1]. It is a kind of appendix to the book. Some familiarity with the earlier book is presupposed. The book itself proposes a complete physical and mathematical blueprint of the brain’s OS. A first addition to the book (see Chapters 5 to 10 below) concerns the relation between the afore-mentioned blueprint and the more than 2000-year-old so-called fundamental laws of thought of logic and philosophy, which came to be viewed as being three (3) in number, namely the laws of 1) Identity, 2) Contradiction, and 3) the Excluded Middle. The blueprint and the laws cannot both be the final foundation of the brain’s OS. The design of the present paper is to interpret the laws in strictly mathematical terms in light of the blueprint. This addition constitutes the bulk of the present article. Chapters 5 to 8 set the stage. Chapters 9 and 10 present a detailed mathematical analysis of the laws. A second addition to the book (Chapter 11) concerns the distinction between the laws and the axioms of the brain’s OS. Laws are part of physics. Axioms are part of mathematics. Since the theory of the brain’s OS involves both physics and mathematics, it exhibits both laws and axioms. A third addition (Chapter 12) to the book involves an additional flavor of digitality in the brain’s OS. In the book, there are five (5). But brain chemistry requires a sixth. It will be called Existence Digitality. A fourth addition (Chapter 13) concerns reflections on the role of imagination in theories of physics in light of the ignorance of deeper causes. Chapters 1 to 4 present preliminary matter, for the most part a brief survey of general concepts derived from what is in the book [1]. Some historical notes are gathered at the end in Chapter 14.

国际标准S-101电子航海图解析研究与实践

国际标准S-101电子航海图数据解析是实现新一代电子航海图显示与应用系统的基础。为促进新一代电子航海图显示与应用系统的开发,提出一种基于ISO 8211库的S-101电子航海图解析方法。该方法利用ISO 8211库读取电子海图数据记录,根据数据记录的首字段名,将记录存储至相应的记录列表,根据数据记录的字段结构,顺序解析数据集通用记录、坐标参考系统记录、信息类型记录、要素类型记录及相关联记录,并构建信息对象和要素对象。运用C++编程语言开发了解析程序,对所提方法的正确性进行了验证,结果表明该方法能正确解析S-101电子航海图,可为新一代电子航海图系统的开发提供参考。

欠发达地区县级公共图书馆法治建设任重道远——对鄂、渝、湘、黔若干县级图书馆的调研分析

文章选取了我国欠发达地区25个县级公共图书馆,围绕《中华人民共和国公共图书馆法》的具体实施情况开展深入调查。发现当前我国欠发达地区县级公共图书馆的法治化治理水平有所提升,但地方政府依法保障的情况仍然堪忧。为此提出了4个基层公共图书馆法治建设着力点,为新时代基层公共图书馆的法治化建设和高质量发展提供参考。

为什么教师是教育必需的?

质疑教师之必要性和重要性的传统主要依据自然主义、自由至上主义、无政府主义以及建构主义的教育理念和教育主张。这种质疑在今天伴随着智能化学习的兴盛,以及教育对技术革新的过度强调而被不断加强。重审人类教育,将教育作为人类独特的伟大事业,教师则是这种事业必不可少的有效主体。在这里,教师是这样的人:他作为人类精神和美好价值的引导者、探索者和保护者,真诚邀请和指引儿童参与、理解、共享人类生活,通过培育人文和理性,实现新来者的身份转变,使他们成为人类生活的合格者。在这个意义上,教师一定是教育必需的。

儿童权利理论视域下特殊儿童阅读推广研究——基于我国32家省级公共图书馆的调查

儿童权利理论为公共图书馆特殊儿童阅读权利保护提供了有力支撑。文章以网络调查法为主,结合文献调查等方法,聚焦公共图书馆特殊儿童阅读发展现状,对我国32家省级公共图书馆特殊儿童阅读服务的现状、特点、经验、成果进行了调查研究,分析儿童权利理论视域下特殊儿童阅读推广存在的困境,提出提升特殊儿童保护意识、提供人性化阅读环境和特殊服务、建设适应特殊儿童的多样化馆藏、组织多元化融合阅读活动等优化对策。

新发展理念下高校图书馆高质量发展研究

文章通过辩证思维引申出,高校图书馆在创新、协调、绿色、开放、共享的新发展理念下高质量发展的主要矛盾是图书馆资源整合、专业素养、服务创新等方面所呈现的综合能力水平与高校发展过程中人才培养、专业建设、科学研究、文化自信等多元化需求之间契合度高低的矛盾。因此,研究认为高校图书馆应树立全局观意识,运用新发展理念下创新理念的全面变革、协调理念的融合发展、绿色理念的生态和谐、开放理念的协同互助、共享理念的价值普惠等五个方面的发展策略,以便在未来的转型与变革中全面实现高质量发展。

AI赋能哲学社会科学研究、传播及评价

人工智能正在改变整个人类社会,丰富人们的文化生活,促进科技创新与发展,是各行各业“新质化”的促进剂。人工智能可为哲学社会科学提供高效的研究工具和研究场景,将深刻影响哲学社会科学研究、传播及评价的方方面面。加速人工智能在哲学社会科学研究、传播及评价领域的深入应用,提升哲学社会科学研究效率,促进哲学社会科学的创新与发展,构建自主知识体系,是当下哲学社会科学工作者的重要使命。为推进AI赋能哲学社会科学(AI4SS),2024年9月28日,由西华大学、四川省社会科学学术期刊协会主办,西华大学学术期刊部、四川省社会科学重点研究基地四川学术成果分析与应用研究中心承办的“AI赋能社科研究与评价天府论坛”在西华大学举行。来自南京大学、中国人民大学、四川大学等高校与科研机构的20位专家学者,从不同学科、研究领域出发,就人工智能赋能哲学社会科学研究、传播及评价进行了深入交流。文章为20位专家学者在论坛上所作报告的主要观点。这些报告议题包括AI赋能人文社科研究方法的变革,法学、教育学、经济学、图书情报学、新闻传播等社会学科视域下的人工智能,AI辅助社会科学研究的学术伦理,人工智能在医疗健康、大宗商品价格风险监测、大学生高阶思维能力培养等方面的应用,AI赋能学术期刊出版与传播,AI赋能哲学社会科学评价新模式、新平台、新场景,等等。这些议题共同描绘了一个由AI技术驱动的多学科融合与创新的新时代。

SIMULINK中自抗扰控制技术自定义模块库的创建

在研究自抗扰控制技术的基础上,以MATLAB/SIMULINK为仿真平台,编写M_Funtion程序实现特殊非线性函数、应用S_Funtion定制跟踪微分器和扩张状态观测器等新型动态系统模块、利用子系统封装技术完成控制律组合和常用自抗扰控制器算法,按照模块化建模思想,创建了用户自定义的自抗扰控制器模块库。通过串级调速自抗扰控制系统的仿真实例,说明利用该模块库,实现了自抗扰控制技术的图形化建模,参数设置修改直观方便,而且创建方法简单易行、可扩充性强,不仅为自抗扰控制技术的仿真研究提供了有效工具,并且对相关领域的仿真模型库扩建具有参考价值。

稀有海洋放线菌Salinispora arenicola大片段DNA基因组文库的构建

目的:构建稀有海洋放线菌S.arenicola基因组文库。方法:以稀有海洋放线菌S.arenicola为实验材料,随机剪切提取的总DNA,5′-磷酸末端补平回收40kb左右的DNA片段,与pWEBTM载体连接,经包装蛋白包装成噬菌体后侵染宿主细胞E.coliEPI100,构建该菌株的基因组文库,并对该文库进行质量鉴定。结果:成功构建了稀有海洋放线菌S.arenicola的基因组文库,效价达6.5×104CFU/mL,得到3000个阳性克隆子,远远大于按覆盖率为99%计算至少所需的657个阳性克隆子数,且平均插入片段长度为36kb,重组率100%。阳性克隆子保存于96孔板中,-80℃保存。结论:所构建文库的各项指标均达到要求,为了进一步评估S.arenicola所能合成的所有潜在天然产物,还需要进一步检测该文库中包含有生物合成基因簇的大肠杆菌的表达情况。

水母雪莲Myb转录调控因子SmP基因的克隆及序列分析

采用TD PCR(TouchdownPCR)法从水母雪莲 (SaussureamedusaMaxim)红色系愈伤组织cDNA文库中筛选并克隆了雪莲Myb转录调控因子SmP (S .medusaMaximMyb relatedPgene)基因。序列分析表明该基因全长 96 9bp ,包括一个 771bp的完整开放阅读框架 (ORF) ,编码一个 2 5 6氨基酸残基的蛋白质。氨基酸序列的同源性分析表明在N 端具有两个典型的R2R3 MybDNA结合结构域。C 端富含亲水的丝氨酸S(18 38% ) ,且以寡聚体的形式存在 。