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Fosmid library construction and screening for the maize mutant gene Vestigial glume 1 被引量:5
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作者 Chaoxian Liu Xiaoli Liu +2 位作者 Lei Lei Haiying Guan Yilin Cai 《The Crop Journal》 SCIE CAS CSCD 2016年第1期55-60,共6页
The maize mutant gene Vestigial glume 1(Vg1) has been fine-mapped to a narrow region by map-based cloning and the candidate gene for Vg1 spanned 19.5 kb. Here we report Vg1 genomic fosmid library construction and scre... The maize mutant gene Vestigial glume 1(Vg1) has been fine-mapped to a narrow region by map-based cloning and the candidate gene for Vg1 spanned 19.5 kb. Here we report Vg1 genomic fosmid library construction and screening. The fosmid library of Vg1 consisted of574,000 clones with an average insert size of 36.4 kb, representing 7.9-fold coverage of the maize genome. Fosmid stability assays indicated that clones were stable during propagation in the fosmid system. Using Vg1 candidate gene-specific primers, a positive clone was successfully identified. This discovery will pave the way for identifying the function of Vg1 in maize development. 展开更多
关键词 VEsTIGIAL GLUME 1 FOsMID library construction library sCREENING
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Construction and selection of the natural immune Fab antibody phage display library from patients with colorectal cancer 被引量:9
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作者 Bao-Ping Wu~1 Bing Xiao~1 Tian-Mo Wan~1 Ya-Li Zhang~1 Zhen-Shu Zhang~1 Dian-Yuan Zhou~1 Zhuo-Sheng Lai~1 Chun-Fang Gao~2 1 Institute for Digestive Diseases,Nanfang Hospital,Guangzhou 510515,Guangdong Province,China2 Surgical Department of Colon and Rectum,150 Central Hospital,Luoyang 471031,Henan Province,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2001年第6期811-815,共5页
AIM: To construct the natural immune Fab antibody phage display libraries of colorectal cancer and to select antibodies related with colorectal cancer. METHODS: Extract total RNA from tissue of local cancer metastasis... AIM: To construct the natural immune Fab antibody phage display libraries of colorectal cancer and to select antibodies related with colorectal cancer. METHODS: Extract total RNA from tissue of local cancer metastasis lymph nodes of patients with colorectal cancer. RT-PCR was used to amplify the heavy chain Fd and light chain kappa and the amplification products were inserted successively into the vector pComb3 to construct the human libraries of Fab antibodies. They were then panned by phage display technology. By means of Dot immunoblotting and ELISA, the libraries were identified and the Fab phage antibodies binding with antigens of colorectal cancer were selected. RESULTS: The amplified fragments of Fd and kappa gained by RT-PCR were about 650 bp. Fd and kappa PCR products were subsequently inserted into the vector pComb3, resulting in a recombination rate of 40% and the volume of Fab phage display library reached 1.48 x 10(6).The libraries were enriched about 120-fold by 3 cycles of adsorption-elution-multiplication (panning). Dot immunoblotting showed Fab expressions on the phage libraries and ELISA showed 5 clones of Fab phage antibodies which had binding activities with antigens of colorectal cancer. CONCLUSION: The natural immune Fab antibody phage display libraries of colorectal cancer were constructed. They could be used to select the relative antibodies of colorectal cancer. 展开更多
关键词 Genes Immunoglobulin Peptide library ANTIBODIEs BACTERIOPHAGEs Colorectal Neoplasms Humans Immunoglobulin Fab Fragments Research support Non-U.s. Gov't
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Construction and characterization of a cDNA library from human liver tissue with chronic hepatitis B 被引量:2
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作者 陈晓红 陈智 +3 位作者 姚航平 陈峰 朱海红 周红娟 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2005年第4期288-294,共7页
Objective: To construct a cDNA library from human liver tissue with chronic hepatitis B and check its quality for investigating the expression level of liver tissue infected by hepatitis B virus. This will then be use... Objective: To construct a cDNA library from human liver tissue with chronic hepatitis B and check its quality for investigating the expression level of liver tissue infected by hepatitis B virus. This will then be used to find the relevant genes and interesting proteins associated with the development of hepatitis B. Methods: The total RNA from liver tissue with chronic hepa- titis B was extracted and the mRNA was purified using TRIZOL method. Switching mechanism at 5′ end of the RNA transcript (SMART) technique and CDS III/3′ primer were used for first-strand cDNA synthesis. Long distance polymerase chain reaction (LD PCR) was then used to synthesize the double-strand cDNA that was then digested by Sfi I and fractionated by CHROMA SPIN-400 column. The longer than 0.4 kb cDNAs were collected and ligated to λTriplEx2 vector. Then λ phage packaging reaction and library amplification were performed. The qualities of both unamplified and amplified cDNA libraries were strictly checked by conventional titer determination. Fourteen plaques were randomly picked and tested using PCR with universal primers derived from the sequence flanking the vector. Results: The titers of unamplifed and amplified libraries were 1.94×106 pfu/ml and 1.49×109 pfu/ml respectively. The percentages of recombinants from both libraries were 98.15% in unamplified library and 98.76% in amplified library. The lengths of the inserts were 1.23 kb in average, 1?2 kb in 64.29%, and 0.5?1.0 kb in 35.71%. Conclusion: A high quality cDNA library from human liver tissue with chronic hepatitis B was successfully constructed. 展开更多
关键词 cDNA library Human liver tissue Chronic hepatitis B construction and characterization
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Construction of human Fab library and screening of a single-domain antibody of amyloid-beta 42 oligomers
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作者 Zuanning Yuan Minge Du +1 位作者 Yiwen Chen Fei Dou 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第33期3107-3115,共9页
Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oli- gomers and constructed a h... Screening humanized antibodies from a human Fab phage display library is an effective and quick method to obtain beta-amyloid oligomers. Thus, the present study prepared amyloid-beta 42 oli- gomers and constructed a have human Fab phage display library based on blood samples from six healthy people. After three rounds of biopanning in vitro, a human single-domain antibody that spe- cifically recognized amyloid-beta 42 oligomers was identified. Western blot and enzyme-linked im- munosorbent assay demonstrated this antibody bound specifically to human amyloid-beta 42 tetramer and nonamer, but not the monomer or high molecular weight oligomers. This study suc- cessfully constructed a human phage display library and screened a single-domain antibody that specifically recognized amyloid-beta 42 oligomers. 展开更多
关键词 neural regeneration AMYLOID-BETA Alzheimer's disease OLIGOMER single-domain antibody phagedisplay antibody library construction ALPHA-sYNUCLEIN Parkinson's disease humanized antibody immunotherapy grants-supported paper NEUROREGENERATION
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An Examination on the Support of Library Information to the Construction of National Research Organizations
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作者 Hao Wang 《Journal of Electronic Research and Application》 2019年第3期1-7,共7页
The country’s economic development determines the political and cultural status.At the present time,China’s rapid economic development has greatly improved the country’s international prominence.Although China has ... The country’s economic development determines the political and cultural status.At the present time,China’s rapid economic development has greatly improved the country’s international prominence.Although China has high economic and political achievements,China’s cultural soft power is still in a weak position.Cultural soft power is of great significance in improving the country’s overall national strength and international competitiveness.Research organizations are an important part of the country’s soft power and are of great significance to the strengthening of national soft power.Research organizations are the“peripheral brain”in the decision-making process of the government.National government agencies need democratic science in making decisions,and the adopted policy methods must have positive and effective outcomes on the people.Therefore,the national government agencies cannot achieve this without the help of research organizations.This resulted in the development and rising construction of research organizations.With the comprehensive development and construction of research organizations,whilst making progress,has also exposed several shortcomings.The construction of China’s research organizations requires various supports;especially the intelligence agencies and access to library information,which has a unique advantage in promoting the construction of research organizations[1].This paper first introduces the definition,classification and characteristics of a research organization.It will also provide a preliminary understanding of research organizations.The paper will then highlight and analyze the problems encountered by the research organization.Finally,through the grasp of the advantages of the library and intelligence agencies,a solution is proposed. 展开更多
关键词 library and Information Research ORGANIZATION construction sUPPORT
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Research on the Construction of Chinese Universities from Intelligent Library to Smart Library
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作者 Yuxuan Zhao 《管理科学与研究(中英文版)》 2020年第1期18-24,共7页
The study aims to discuss the construction and development of university library,and transform the management and operation mode of university library,thereby realizing the transformation and upgrading to smart librar... The study aims to discuss the construction and development of university library,and transform the management and operation mode of university library,thereby realizing the transformation and upgrading to smart library.Taking Anhui University of Finance and Economics as the research object,first,based on the literature and data combined with questionnaire design and expert interview,data statistics was made on the development of smart library.Second,analytical hierarchy process(AHP)and big data analysis were applied to complete the construction of the smart library system.Third,based on the data analysis method,the problems in the construction of the smart library were discussed.The results showed that the Chinese smart library started late and developed rapidly after 2012;from information perception to smart service,the construction of smart library system had realized the transformation and upgrading of library construction;the key to solve the construction of smart library was to standardize library construction,realize resource co-construction and sharing,promote technological innovation,and strengthen the construction of smart librarians.To sum up,the construction of smart library is expected to make the library and librarians become the cooperative objects of teaching and scientific research in colleges and universities. 展开更多
关键词 UNIVERsITY smart library system construction Hierarchical Model smart service
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Based on the Construction of University Library and the Evaluation of Space Service Quality
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作者 Wan Ying 《Journal of Contemporary Educational Research》 2021年第1期65-69,共5页
In order to strengthen the construction of university library,promote the sustainable development of university library,and provide a good library space environment for university students,this paper expounds the qual... In order to strengthen the construction of university library,promote the sustainable development of university library,and provide a good library space environment for university students,this paper expounds the quality evaluation of university library by establishing the evaluation index of space service quality,and puts forward the ways to improve the service quality of University Library for reference. 展开更多
关键词 Colleges and universities library construction space services Quality evaluation
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The plight and Countermeasures brought to the constructions of library literature by effect of college enrollment
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作者 Xiaoyan Fan Yu Chen 《International Journal of Technology Management》 2013年第8期35-37,共3页
This text introduces the concept of local literature and collection range, explains the significance of literature collection and explores building programs of local university library. Currently, university library g... This text introduces the concept of local literature and collection range, explains the significance of literature collection and explores building programs of local university library. Currently, university library generally put a strong emphasis on collection of academic literature, but has less attention on literature collections which reflect the situation of university location, or even worse, simply don' t launch business in this field. For the university library, it is a great loss of literature information resources, as well as a great regret of library work. First, what is the local literature? It is literature data which document a region' s past and present issues like politics, economy, culture, education, history, geography and other aspects like important characters and events, customs, folklore etc. 展开更多
关键词 college enrollment quality of education library construction
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Development and construction of China's higher education libraries
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作者 YAN Jinwei ZHENG Lan +4 位作者 SONG Xue ZHANG Yan SONG Jiao DAI Longji LI Dejuan 《Chinese Journal of Library and Information Science》 2008年第2期31-51,共21页
Libraries in China's higher education institutions have been developing in keeping pace with the flourishing development of China's higher education. This article aims to make an introduction to the constructi... Libraries in China's higher education institutions have been developing in keeping pace with the flourishing development of China's higher education. This article aims to make an introduction to the construction of China's higher education libraries, especially the recent three decades' achievements since China's reform and opening-up in 1978. In this article, the authors draw a general picture of the development of libraries in China's higher education institutions, covering such eight aspects as management, types and positioning,organizational structure and personnel, expenditure and buildings, reader service, building and sharing of resources as well as automation system. 展开更多
关键词 Universities and colleges library library in China's higher education institutions library cause library construction Academic library
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Construction and screening of suppression subtractive hybridization library of renal cell carcinoma
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作者 张勇 《外科研究与新技术》 2003年第2期114-114,共1页
Objective To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC). Methods Poly A+ RNA was isolated from RCC lines 786-O(tester) and renal cell(RC) lines HK-... Objective To construct and screen the suppression subtractive hybridization (SSH) library of human renal cell carcinoma (RCC). Methods Poly A+ RNA was isolated from RCC lines 786-O(tester) and renal cell(RC) lines HK-2 ( driver), respectiely. SSH procedure was performed according to the protocol of the PCR-Select cDNA Subtraction Kit ( Clontech), and PCR products were cloned into pT-Adv vector and transformed E. coli TOP10F’. All positive clones picked out were digested and some of which were sequenced. Results The SSH library contained 362 clones with SSH cDNA fragments distributed mainly from 0.3 to 0.9 kb. Among 50 clones sequenced randomly,2 represented unknown genes and the other 48 derived from 36 known genes. Conclusion The quality of the SSH library of human RCC is reliable and is construction is the basis for further screening differentially expressed genes of RCC. 6 refs,4 figs, 1 tab. 展开更多
关键词 of construction and screening of suppression subtractive hybridization library of renal cell carcinoma
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Urban Construction Land Suitability Evaluation Based on Improved Multi-criteria Evaluation Based on GIS(MCE-GIS): Case of New Hefei City,China 被引量:23
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作者 ZHANG Xiaorui FANG Chuanglin +1 位作者 WANG Zhenbo MA Haitao 《Chinese Geographical Science》 SCIE CSCD 2013年第6期740-753,共14页
Urban construction land suitability evaluation (UCLSE) is a complex system engineering and the basis for rational use of the limited urban land resources in China. It has an important practical value on urban constr... Urban construction land suitability evaluation (UCLSE) is a complex system engineering and the basis for rational use of the limited urban land resources in China. It has an important practical value on urban construction land use planning and management from the angle of methodology. As a widely used technique, traditional multi-criteria evaluation based on GIS (MCE-GIS), is not suitable for UCLSE. This study develops an improved MCE-GIS method which could be more suitable for UCLSE based on urban complex ecological system theory and the summary of the shortcomings of traditional MCE-GIS. The improvements include three aspects: a composite evaluation index system rather than natural indexes alone, an index weight calculated by using fuzzy Analytic Hierarchy Process (AHP) method rather than the common AHP method, and the integrated overlay rule, which includes selecting the minimum value, weighted linear combination (WLC) and simple summation. The main advantage of this improved technique is that it can make UCLSE more comprehensive, more operational and more reasonable. It can provide a scientific basis for decision making in the planning and management of urban construction land use. The improved MCE-GIS system has been adopted in the New Hefei City, Anhui Province, China. Based on the results of UCLSE in New Hefei, three functional areas including construction-appropriate areas, construction-restricted areas and construction-forbidden areas could be worked out, in which 36.90% of the total study area could be developed as urban construction land and the remaining 63.10% should be protected as reserves land or as ecological land. Furthermore, the results can orovide scientific decision suooort for spatial planning and eco-environment nrotection in New Hefei. 展开更多
关键词 urban construction land suitability evaluation (UCLsE) multi-criteria evaluation based on GIs (MCE-G1s New Hefei
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Rural Tourism, Shanxi Merchants’ Culture and New Rural Construction of Socialism
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作者 史琨 《Journal of Landscape Research》 2010年第8期76-80,共5页
Rural tourism was a new form of tourism gradually emerging in China in recent years. It satisfied urban residents' pursuit of nature and had a promising market prospect. New rural construction of socialism was an ... Rural tourism was a new form of tourism gradually emerging in China in recent years. It satisfied urban residents' pursuit of nature and had a promising market prospect. New rural construction of socialism was an important historical task in the process of economic and social development of China at present and in future period. New rural construction was the important basis of rural tourism development and meanwhile rural tourism development could promote new rural construction. Shanxi Merchant's Culture of Xi Village in Jinzhong City was a kind of excellent rural tourism resource. Developing rural tourism was one of the effective ways to promote new rural construction of socialism in Xi Village. 展开更多
关键词 RURAL TOURIsM sHANXI Merchant’s CULTURE New RURAL construction
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Strategies for Prioritizing Ecological Construction with Consideration of Agricultural Industry Development in Northern China’s Agro- pastoral Ecotone : A Perspective of Opportunity Cost 被引量:2
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作者 Liang REN Fang SUN Ding DING 《Asian Agricultural Research》 2021年第9期6-12,19,共8页
In northern China’s agro-pastoral ecotone,there are conflicts between agricultural industry and ecological construction resource competition,and also problems urgently to be solved for economic growth and sustainable... In northern China’s agro-pastoral ecotone,there are conflicts between agricultural industry and ecological construction resource competition,and also problems urgently to be solved for economic growth and sustainable ecological improvement.Taking the development concept of"lucid waters and lush mountains being mountains of gold and silver"as the guiding principle of prioritizing ecological and green development,this paper studied the strategies for prioritizing ecological construction with consideration of economic development of agricultural industry from the perspective of opportunity cost.Through the analysis of opportunity cost,externality,and Pareto optimal theory,and using the calculation method of the unit equivalent factor value of terrestrial ecological service function,this paper calculated the ecological service value of forest,grassland,and farmland.It found that the ecological value of forest and grassland per unit area was greater than the ecological value of agricultural industry.This indicates that the opportunity cost of farmland for ecological construction was less than that of agricultural industry.Based on the theoretical and quantitative analysis,it came up with recommendations for establishing a comprehensive system for prioritizing ecological construction with consideration of agricultural industry development and establishing and improving the incentive mechanism of ecological construction. 展开更多
关键词 Opportunity cost Northern China’s agro-pastoral ecotone Agricultural industry Ecological construction Coordinated development
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China's Human Rights Construction Centers on People's Livelihood 被引量:1
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作者 LUO HAOCAI 《The Journal of Human Rights》 2014年第1期2-5,共4页
1. Concept of People's Livelihood in Traditional Chinese Culture The concept of human rights was borrowed from the West. In order to have it become rooted in China, people needed to find traces in Chinese culture on ... 1. Concept of People's Livelihood in Traditional Chinese Culture The concept of human rights was borrowed from the West. In order to have it become rooted in China, people needed to find traces in Chinese culture on which the human rights theoretical system could be based and the Chinese human rights idea could be realized. 展开更多
关键词 CPC China’s Human Rights construction Centers on People’s Livelihood
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The 21^(st) century system reform and development mode of construction management education (CME) in China
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作者 叶晓甦 《Journal of Chongqing University》 CAS 2004年第2期81-85,共5页
With China’s entry into WTO and the development of the economic globalization, market competition in the construction field is more and more featured by the competition of talents, the core of which is the cultivatio... With China’s entry into WTO and the development of the economic globalization, market competition in the construction field is more and more featured by the competition of talents, the core of which is the cultivation mechanism innovation of higher construction management talents. The approach to meet the requirement of this situation is to adopt innovation ideas, improve the supply-demand structure of CME, reform the education system to relate theory to practice, pay attention to the needs of the educated, enhance global communication and cooperation, and improve the knowledge structure of teachers. 展开更多
关键词 construction management education (CME) China’s higher education GLOBALIZATION
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Features of Egypt’s Water Transportation and China’s Participation in Egypt’s Port Construction
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作者 ZHAO Jun HU Yu 《International Relations and Diplomacy》 2018年第9期469-475,共7页
There are four distinctive features in Egypt’s port construction and maritime transportation:Egypt’s port construction and maritime transportation is a vital part of the international shipping system;maritime transp... There are four distinctive features in Egypt’s port construction and maritime transportation:Egypt’s port construction and maritime transportation is a vital part of the international shipping system;maritime transportation is an important backbone industry in Egypt;Egypt’s port construction propels its domestic economy and international interaction;and the capacity for ocean shipping still needs upgrading.China’s participation in Egypt’s port construction is,on one side,the further demand of China’s economic globalization,and on the other side,it is an outcome of the beneficial interactions between China and Egypt.Egypt’s port construction has started a new chapter ever since Abdel Fattah al Sisi assumed power.As opportunities and risks coexist in China’s participation in Egypt’s port construction,only when we are fully aware of the risks and make scientific decisions based on China’s own strengths can we effectively participate in Egypt’s port construction to yield a win-win result. 展开更多
关键词 the BELT and ROAD INITIATIVE Egypt’s PORT construction risk PREVENTION
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Construction Strategies of Public Activity Spaces in Residential Districts Based on Children's Activities
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作者 XIAO Lu LIN Hui ZHANG Minglin 《Journal of Landscape Research》 2016年第2期49-51,54,共4页
The relationship between children's activities and public spaces was analyzed and summarized from three perspectives, namely, children's demands on outdoor activity spaces, factors infl uencing children's ... The relationship between children's activities and public spaces was analyzed and summarized from three perspectives, namely, children's demands on outdoor activity spaces, factors infl uencing children's behavior requirements, and planning strategies. The paper provided theoretical support for exploring the relationship between children's activities and public spaces, and proposed planning strategies for improving use effi ciency of urban outdoor public spaces and facilities, and creating public spaces capable of meeting children's psychological needs. 展开更多
关键词 Children’s activities Residential district spatial form construction strategies
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People's Construction Bank of China
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作者 Xiao Jun 《China's Foreign Trade》 1995年第1期37-38,共2页
On the eve of the grand celebrations marking the 45th anniversary for the founding of new China, the People’s Construction Bank of China, one of our country’s four pillars of the banking industry, welcomed its 40th ... On the eve of the grand celebrations marking the 45th anniversary for the founding of new China, the People’s Construction Bank of China, one of our country’s four pillars of the banking industry, welcomed its 40th birthday. The Government Administration Council decided on September 9, 1954, to establish the People’s Construction Bank of China to handle mainly appropriations for capital construction and floating capital loans for construction enterprises toexercise financial supervision of the rational use 展开更多
关键词 this OVER People’s construction Bank of China BANK
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Research on the Construction of Social Mechanism for the Realization of China’s “Sustainable Overseas Interest”
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作者 Lyu Xiaoli Liu Jingwen Hu Xu 《International Understanding》 2019年第3期35-38,共4页
China’s overseas interest has become more visible and fragile with the deepening of integration into the world.To protect China’s overseas interest,we need to focus on the realization of China’s“sustainable overse... China’s overseas interest has become more visible and fragile with the deepening of integration into the world.To protect China’s overseas interest,we need to focus on the realization of China’s“sustainable overseas interest”in addition to the prevention of direct infringement and threat.“Sustainable overseas interest”include different content levels of nation,corporate and society.Referring to the experience of other countries,we find that China still lacks the main body dimension in its maintenance mechanism of“sustainable overseas interest”,and the social mechanism level of the trinity system of government,enterprise and society is still insufficient. 展开更多
关键词 the construction of social Mechanism the REALIZATION of China’s sUsTAINABLE OVERsEAs INTEREsT
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The Use of Materials from Biomass as Construction Materials
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作者 Francesca Giglio 《Open Journal of Civil Engineering》 2013年第2期82-84,共3页
The paper describes some studies conducted by the author, about low impact materials and, particularly, on alternative uses of biomass materials for building materials. Italy needs to develop renewable energy sources ... The paper describes some studies conducted by the author, about low impact materials and, particularly, on alternative uses of biomass materials for building materials. Italy needs to develop renewable energy sources and agriculture offers many opportunities: biomass, vegetable residues for livestock breeding, food industry waste, organic waste, solid biofuels, biogas, and biofuels, will be the new renewable energy sources. Researches for promoting sustainable development, including chemistry field are increasing interest in the exploitation of vegetal biomass, in particular waste as a raw material for production of bio-products and biofuels. Not always, however, the use of waste materials such as biomass is the most appropriate, or in any case, the one with less energy expenditure. As ever more evident, in fact, the construction sector is approaching the possibility of using these materials for other purposes, combining the demands of saving natural resources, the need for energy efficiency in buildings and production processes. 展开更多
关键词 ECO-INNOVATION BIOMAss and construction Hemp’s MAsONRY
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