The use of artificial diets to mass reared insect stock under laboratory conditions, promotes knowledge about the biology, behavior, and nutritional requirements of insects;with such information being fundamental for ...The use of artificial diets to mass reared insect stock under laboratory conditions, promotes knowledge about the biology, behavior, and nutritional requirements of insects;with such information being fundamental for the formulation of efficient integrated pest management programs (IPM) strategies. However, the artificial diets used for rearing Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) in the laboratory in this study, do not contain wheat germ, used for routine laboratory rearing. The aim of this study was to compare food preference and consumption by S. frugiperda larvae, as well as evaluate insect developmental parameters in the standard diet and the bean-based diets under laboratory conditions. Four artificial diets were used: a standard diet based on broad beans (Phaseolus vulgaris L.) used to rear S. frugiperda (D1), a diet with the substitution of two types of beans, cowpea (Vigna unguiculata L.) (D2) and pigeon pea (Cajanus cajan L) (D3);and a diet of fresh corn leaves (D4), the host plant of the pest species. Using a multiple-choice test, we observed that the larvae preferred diets D4 followed by D1. The relative consumption rate (RCR), relative growth rate (RGR), relative metabolic rate (RMR), approximate digestibility (AD), efficiency of conversion of ingested food (ECI), efficiency of conversion of digested food (ECD), and the metabolic cost (CM) for S. frugiperda fifth instar larvae varied among diets. The diet D4 resulted in better ingestion, digestion, assimilation, and conversion of food, but with a metabolic cost to assimilate the food. The different bean varieties present in the diet influenced larval length, larval periods, larval survival, pupal periods, sex ratio, pupal weight, and pupal survival;but fecundity and longevity of adults were not affected. The different bean-based diets did positively influence population growth, with the most outstanding being D4. From the results of this study, the most adequate diet for rearing S. frugiperda in the laboratory is D4.展开更多
【目的】研究实验室条件下韭蛆Bradysia odoriphaga Yang et Zhang大量饲养的方法以解决试虫不足的问题。【方法】本实验在25℃,相对湿度75%,光周期16L∶8D条件下,通过控制变量法筛选出人工饲料配方,运用两性生命表软件,分析比较人工饲...【目的】研究实验室条件下韭蛆Bradysia odoriphaga Yang et Zhang大量饲养的方法以解决试虫不足的问题。【方法】本实验在25℃,相对湿度75%,光周期16L∶8D条件下,通过控制变量法筛选出人工饲料配方,运用两性生命表软件,分析比较人工饲料与韭菜Allium tuberosum的饲喂效果。【结果】对不同成分和不同含量的8种韭蛆人工饲料配方筛选,结果确定最优的人工饲料配方成分和含量为:平菇粉6 g,韭菜粉5 g,琼脂粉1.25 g,山梨酸0.04 g,苯甲酸0.05 g,酵母粉0.5 g,维生素C 2.5 g和水50 m L。该配方和韭菜饲养效果相当,不存在显著差异(P>0.05)。同时,两性生命表结果表明,人工饲料饲喂的韭蛆平均世代周期23.6800 d,韭菜饲喂的韭蛆世代周期为25.9000 d,两者之间差异显著(P<0.05)。生殖生物学特性分析发现:韭菜和人工饲料饲喂的韭蛆雌成虫净生殖率分别为16.3200和41.1800/d,内禀增长率分别为0.1078和0.1570/d。【结论】筛选的人工饲料和韭菜均可以稳定地完成韭蛆的世代繁殖,且人工饲料饲喂效果略优于韭菜,另外人工饲料较韭菜不但具有低成本省人工的优点,而且可解决韭菜反季节供应和农药残留问题,保证稳定的虫源供给。展开更多
文摘The use of artificial diets to mass reared insect stock under laboratory conditions, promotes knowledge about the biology, behavior, and nutritional requirements of insects;with such information being fundamental for the formulation of efficient integrated pest management programs (IPM) strategies. However, the artificial diets used for rearing Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) in the laboratory in this study, do not contain wheat germ, used for routine laboratory rearing. The aim of this study was to compare food preference and consumption by S. frugiperda larvae, as well as evaluate insect developmental parameters in the standard diet and the bean-based diets under laboratory conditions. Four artificial diets were used: a standard diet based on broad beans (Phaseolus vulgaris L.) used to rear S. frugiperda (D1), a diet with the substitution of two types of beans, cowpea (Vigna unguiculata L.) (D2) and pigeon pea (Cajanus cajan L) (D3);and a diet of fresh corn leaves (D4), the host plant of the pest species. Using a multiple-choice test, we observed that the larvae preferred diets D4 followed by D1. The relative consumption rate (RCR), relative growth rate (RGR), relative metabolic rate (RMR), approximate digestibility (AD), efficiency of conversion of ingested food (ECI), efficiency of conversion of digested food (ECD), and the metabolic cost (CM) for S. frugiperda fifth instar larvae varied among diets. The diet D4 resulted in better ingestion, digestion, assimilation, and conversion of food, but with a metabolic cost to assimilate the food. The different bean varieties present in the diet influenced larval length, larval periods, larval survival, pupal periods, sex ratio, pupal weight, and pupal survival;but fecundity and longevity of adults were not affected. The different bean-based diets did positively influence population growth, with the most outstanding being D4. From the results of this study, the most adequate diet for rearing S. frugiperda in the laboratory is D4.
