The common marmoset （Callithrix jacchus） has attracted extensive attention for use as a non-human primate model in biomedical research, especially in the study of neuropsychiatric disorders. However, behavioral test...The common marmoset （Callithrix jacchus） has attracted extensive attention for use as a non-human primate model in biomedical research, especially in the study of neuropsychiatric disorders. However, behavioral test methods are still limited in the field of marmoset research. The light-dark box is widely used for the evaluation of anxiety in rodents, but little is known about light-dark preference in marmosets. Here, we modified the light-dark test to study this behavior. The modified apparatus consisted of three compartments： one transparent open area and two closed opaque compartments. The closed compartments could be dark or light. We found that both adult and young marmosets liked to explore the open area, but the young animals showed more interest than adults. Furthermore, when one of the closed compartments was light and the other dark, the adult marmosets showed a preference for the dark compartment, but the young animals had no preference. These results suggest that the exploratory behavior and the light-dark preference in marmosets are age-dependent. Our study provides a new method to study exploration, anxiety, and fear in marmosets.展开更多
目的探讨旷场实验（open field test，OFT）和明暗箱实验（1ight—darkbox，LDB）作为昆明小鼠状态焦虑动物模型的相关性。方法20只成年雄性昆明小鼠先后放入旷场敞箱和明暗穿梭箱，摄像系统记录5rain的行为变化，实验间隔1周，实验参...目的探讨旷场实验（open field test，OFT）和明暗箱实验（1ight—darkbox，LDB）作为昆明小鼠状态焦虑动物模型的相关性。方法20只成年雄性昆明小鼠先后放入旷场敞箱和明暗穿梭箱，摄像系统记录5rain的行为变化，实验间隔1周，实验参数如下：OFT中央区停留时间百分率（OFT-Ctime％）、中央区水平运动百分率（OFT—Ccross％）、总水平运动得分（OFT—Cross）、总垂直运动得分（OFT—Rear）和粪便粒数得分（OFT-FB），LDB明区停留时间百分率（LDB-Ltime％）、明区水平运动百分率（LDB—Lcross％）、明区垂直运动百分率（LDB-Lrear％）、穿梭次数得分（LDB—Transition）、总水平运动得分（LDB．Cross）、总垂直运动得分（LDB—Rear）和粪便粒数得分（LDB-FB）；采用因子分析、聚类分析和相关分析统计方法进行数据处理。结果①因子分析和聚类分析提示，OFT／LDB独立评价时具有相似3维结构：OFT／LDB焦虑因子、OFT／LDB活性因子和OFT／LDB情绪因子；OFT-LDB合并评价时则为五维结构：LDB焦虑因子、OFT焦虑因子、LDB活性因子、OFT-LDB情绪因子和OFT活性因子。②相关分析提示，OFT情绪因子与LDB情绪因子具有较好相关性（Pearson=0．383，P〈0．05）；LDB焦虑因子与LDB焦虑因子（Pearson=0．989，P〈0．01），OFT焦虑因子与OFT焦虑因子（Pearson=0．934，P〈0．01），LDB焦虑因子与LDB焦虑因子（Pearson=0．956，P〈0．01），OFT／LDB情绪因子与OFT—LDB情绪因子（Pearson=0．835，P〈0．01；Pearson=0．696，P〈0．01），OFT活性因子与OFT活性因子（Pearson=0．926，P〈0．01）具有较好相关性。结论OFT／LDB独立评价时具有相似三维结构，而OFT—LDB合并评价时则为5维结构：仅OFT／LDB情绪因子可以合并。因此，在以“行为组学”联合评价时，各维度并非一一对应，应注意动物模型异质性问题。展开更多
基金supported by the Youth Innovation Promotion Association of the Chinese Academy of Sciences and an SA-SIBS scholarshipthe National Basic Research Development Program (973 Program) of China (2011CBA00400)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB02020100)
文摘The common marmoset （Callithrix jacchus） has attracted extensive attention for use as a non-human primate model in biomedical research, especially in the study of neuropsychiatric disorders. However, behavioral test methods are still limited in the field of marmoset research. The light-dark box is widely used for the evaluation of anxiety in rodents, but little is known about light-dark preference in marmosets. Here, we modified the light-dark test to study this behavior. The modified apparatus consisted of three compartments： one transparent open area and two closed opaque compartments. The closed compartments could be dark or light. We found that both adult and young marmosets liked to explore the open area, but the young animals showed more interest than adults. Furthermore, when one of the closed compartments was light and the other dark, the adult marmosets showed a preference for the dark compartment, but the young animals had no preference. These results suggest that the exploratory behavior and the light-dark preference in marmosets are age-dependent. Our study provides a new method to study exploration, anxiety, and fear in marmosets.
文摘目的探讨旷场实验（open field test，OFT）和明暗箱实验（1ight—darkbox，LDB）作为昆明小鼠状态焦虑动物模型的相关性。方法20只成年雄性昆明小鼠先后放入旷场敞箱和明暗穿梭箱，摄像系统记录5rain的行为变化，实验间隔1周，实验参数如下：OFT中央区停留时间百分率（OFT-Ctime％）、中央区水平运动百分率（OFT—Ccross％）、总水平运动得分（OFT—Cross）、总垂直运动得分（OFT—Rear）和粪便粒数得分（OFT-FB），LDB明区停留时间百分率（LDB-Ltime％）、明区水平运动百分率（LDB—Lcross％）、明区垂直运动百分率（LDB-Lrear％）、穿梭次数得分（LDB—Transition）、总水平运动得分（LDB．Cross）、总垂直运动得分（LDB—Rear）和粪便粒数得分（LDB-FB）；采用因子分析、聚类分析和相关分析统计方法进行数据处理。结果①因子分析和聚类分析提示，OFT／LDB独立评价时具有相似3维结构：OFT／LDB焦虑因子、OFT／LDB活性因子和OFT／LDB情绪因子；OFT-LDB合并评价时则为五维结构：LDB焦虑因子、OFT焦虑因子、LDB活性因子、OFT-LDB情绪因子和OFT活性因子。②相关分析提示，OFT情绪因子与LDB情绪因子具有较好相关性（Pearson=0．383，P〈0．05）；LDB焦虑因子与LDB焦虑因子（Pearson=0．989，P〈0．01），OFT焦虑因子与OFT焦虑因子（Pearson=0．934，P〈0．01），LDB焦虑因子与LDB焦虑因子（Pearson=0．956，P〈0．01），OFT／LDB情绪因子与OFT—LDB情绪因子（Pearson=0．835，P〈0．01；Pearson=0．696，P〈0．01），OFT活性因子与OFT活性因子（Pearson=0．926，P〈0．01）具有较好相关性。结论OFT／LDB独立评价时具有相似三维结构，而OFT—LDB合并评价时则为5维结构：仅OFT／LDB情绪因子可以合并。因此，在以“行为组学”联合评价时，各维度并非一一对应，应注意动物模型异质性问题。
