This paper presents a novel biosensor for bitter substance detection on the basis of light addressable potentiometric sensor(LAPS).Taste receptor cells(TRCs)were used as sensitive elements,which can respond to differe...This paper presents a novel biosensor for bitter substance detection on the basis of light addressable potentiometric sensor(LAPS).Taste receptor cells(TRCs)were used as sensitive elements,which can respond to different bitter stimuli with extreme high sensitivity and speci-ficity.TRCs were isolated from the taste buds of rats and cultured on the surface of LAPS chip.Due to the unique advantages such as single-cell recording,light addressable capability,and noninvasiveness,LAPS chip was used as secondary transducer to monitor the responses of TRCs by recording extracelluar potential changes.The results indicate LAPS chip can effectively record the responses of TRCs to different bitter substances used in this study in a real-time manner for a long-term.In addition,by performing principal component analysis on the LAPS recording data,different bitter substances tested can be successfully discriminated.It is suggested this TRCsLAPS hybrid biosensor could be a valuable tool for bitter substance detection.With further improvement and novel design,it has great potentials to be applied in both basic research and practical applications related to bitter taste detection.展开更多
<正>A novel neurochip based on light addressable potentiometric sensor (LAPS) is designed.Using its light addressable characteristic.The problems of the limitations of restricted discrete active sites of current...<正>A novel neurochip based on light addressable potentiometric sensor (LAPS) is designed.Using its light addressable characteristic.The problems of the limitations of restricted discrete active sites of current neurochips,such as microelectrode array and field effect transistor array can be settled easily.Based on the theoretical analysis of the interface between cells and LAPS,spontaneously discharges of hippocampal neurons induced by Mg~ 2+)-free media treatment were recorded by LAPS.The results demonstrate that this kind of neurochip has potential to monitor electrophysiology of cultured cells in a non-invasive way.展开更多
In this study, “homeostasis”, the function by which living things keep their constancy, was emulated as a lighting control for a building space. The algorithm we developed mimics the mechanisms of the endocrine and ...In this study, “homeostasis”, the function by which living things keep their constancy, was emulated as a lighting control for a building space. The algorithm we developed mimics the mechanisms of the endocrine and immune systems. The endocrine system transmits information entirely, whereas the immune system transmits information with a concentration gradient. A lighting control system using the proposed algorithm was evaluated in a simulation and experiment using a sensor agent robot. In this algorithm, a robot recognizes a person’s behavior and uses it to decide his or her preference as to the illuminance. The results indicate that the algorithm can be used to realize a comfortable lighting control in several situations.展开更多
In order to reach the objective of intelligence and energy saving for university classroom lighting, energy saving lighting control system in university classroom based on wireless sensor network is designed, includin...In order to reach the objective of intelligence and energy saving for university classroom lighting, energy saving lighting control system in university classroom based on wireless sensor network is designed, including design of sensor node and sink, as well as corresponding development of control program and upper-computer software. The system sets single-chip Ameg16 as control center, realizes communication between nodes via nRF24L01 wireless transceiver module, and realizes communication between sink and upper computer via w5100 wireless internet module. It perceives illumination intensity via photoconductor, detects the human body position via infrared pyroelectric sensor, and places the sensor node on the lamp, so the light can be controlled according to position of human body and current illumination intensity, which can realize energy saving to a large extent on condition that lighting requirement is satisfied. The system has low cost, and there is no need to change the original lighting circuit. The light can be turned off by hand, and when multi-media are used for the class, light can keep off even it is dim. In addition, this system has the function of automatic fault report, which is convenient for property maintenance.展开更多
The problem of traffic congestion is a significant phenomenon that has had a substantial impact on the transportation system within the country. This phenomenon has given rise to numerous intricacies, particularly in ...The problem of traffic congestion is a significant phenomenon that has had a substantial impact on the transportation system within the country. This phenomenon has given rise to numerous intricacies, particularly in instances where emergency situations occur at traffic light intersections that are consistently congested with a high volume of vehicles. This implementation of a traffic light controller system is designed with the intention of addressing this problem. The purpose of the system was to facilitate the operation of a 3-way traffic control light and provide priority to emergency vehicles using a Radio Frequency Identification (RFID) sensor and Reduced Instruction Set Computing (RISC) Architecture Based Microcontroller. This research work involved designing a system to mitigate the occurrence of accidents commonly observed at traffic light intersections, where vehicles often need to maneuver in order to make way for emergency vehicles following a designated route. The research effectively achieved the analysis, simulation and implementation of wireless communication devices for traffic light control. The implemented prototype utilizes RFID transmission, operates in conjunction with the sequential mode of traffic lights to alter the traffic light sequence accordingly and reverts the traffic lights back to their normal sequence after the emergency vehicle has passed the traffic lights.展开更多
The character of droplet transfer arc light sense signal is studied and validated in oscillating feed mode. And a novel mini photoelectric arc light sensor together with the controlling circuit used to automatically...The character of droplet transfer arc light sense signal is studied and validated in oscillating feed mode. And a novel mini photoelectric arc light sensor together with the controlling circuit used to automatically stabilize the signal range is developed. Further more the automatically identifying of the droplet transfer character signal from arc light is realized reliably.展开更多
Based on the characteristics of line structured light sensor, a speedy method for the calibration was established. With the coplanar reference target, the spacial pose between camera and optical plane can be calibrate...Based on the characteristics of line structured light sensor, a speedy method for the calibration was established. With the coplanar reference target, the spacial pose between camera and optical plane can be calibrated by using of the camera’s projective center and the light’s information in the camera’s image surface. Without striction to the movement of the coplanar reference target and assistant adjustment equipment, this calibration method can be implemented. This method has been used and decreased the cost of calibration equipment, simplified the calibration procedure, improved calibration efficiency. Using experiment, the sensor can attain relative accuracy about 0.5%, which indicates the rationality and effectivity of this method.展开更多
A multiplexed white light interferometric fiber optic twin-sensor-array was designed to monitor the structural health of large buildings.In this sensing system,based on a Michelson interferometer,an optical path match...A multiplexed white light interferometric fiber optic twin-sensor-array was designed to monitor the structural health of large buildings.In this sensing system,based on a Michelson interferometer,an optical path matching technique is used to demodulate each twin-sensor.Each twin-sensor-array consists of a 2×N sensing element linked by a 3 dB coupler.When one of the twin-sensor is used to measure strain,variations caused by temperature can be compensated for by referencing the other twin-sensor.The multiplexing capacity of the sensing scheme has been analyzed and experimental results with a 2×3 twin-sensor-array are given.展开更多
基金This work was supported by the grants from the National Natural Science Foundation of China(Grant Nos.60725102,31000448)the China Postdoctoral Science Foundation(Grant Nos.20100471737,201104734)the Key Project of Zhejiang Province(Grant No.2010C14006).
文摘This paper presents a novel biosensor for bitter substance detection on the basis of light addressable potentiometric sensor(LAPS).Taste receptor cells(TRCs)were used as sensitive elements,which can respond to different bitter stimuli with extreme high sensitivity and speci-ficity.TRCs were isolated from the taste buds of rats and cultured on the surface of LAPS chip.Due to the unique advantages such as single-cell recording,light addressable capability,and noninvasiveness,LAPS chip was used as secondary transducer to monitor the responses of TRCs by recording extracelluar potential changes.The results indicate LAPS chip can effectively record the responses of TRCs to different bitter substances used in this study in a real-time manner for a long-term.In addition,by performing principal component analysis on the LAPS recording data,different bitter substances tested can be successfully discriminated.It is suggested this TRCsLAPS hybrid biosensor could be a valuable tool for bitter substance detection.With further improvement and novel design,it has great potentials to be applied in both basic research and practical applications related to bitter taste detection.
基金supported by the National Natural Science Foundation of China(Grant Nos.30270387,No.30570492)the Project of State Key Laboratory of Transducer Technology of China(Grant No.SKT0403)the Foundation for the Bureau of Zhejiang Province of China(Grant No.20040197).
文摘<正>A novel neurochip based on light addressable potentiometric sensor (LAPS) is designed.Using its light addressable characteristic.The problems of the limitations of restricted discrete active sites of current neurochips,such as microelectrode array and field effect transistor array can be settled easily.Based on the theoretical analysis of the interface between cells and LAPS,spontaneously discharges of hippocampal neurons induced by Mg~ 2+)-free media treatment were recorded by LAPS.The results demonstrate that this kind of neurochip has potential to monitor electrophysiology of cultured cells in a non-invasive way.
文摘In this study, “homeostasis”, the function by which living things keep their constancy, was emulated as a lighting control for a building space. The algorithm we developed mimics the mechanisms of the endocrine and immune systems. The endocrine system transmits information entirely, whereas the immune system transmits information with a concentration gradient. A lighting control system using the proposed algorithm was evaluated in a simulation and experiment using a sensor agent robot. In this algorithm, a robot recognizes a person’s behavior and uses it to decide his or her preference as to the illuminance. The results indicate that the algorithm can be used to realize a comfortable lighting control in several situations.
文摘In order to reach the objective of intelligence and energy saving for university classroom lighting, energy saving lighting control system in university classroom based on wireless sensor network is designed, including design of sensor node and sink, as well as corresponding development of control program and upper-computer software. The system sets single-chip Ameg16 as control center, realizes communication between nodes via nRF24L01 wireless transceiver module, and realizes communication between sink and upper computer via w5100 wireless internet module. It perceives illumination intensity via photoconductor, detects the human body position via infrared pyroelectric sensor, and places the sensor node on the lamp, so the light can be controlled according to position of human body and current illumination intensity, which can realize energy saving to a large extent on condition that lighting requirement is satisfied. The system has low cost, and there is no need to change the original lighting circuit. The light can be turned off by hand, and when multi-media are used for the class, light can keep off even it is dim. In addition, this system has the function of automatic fault report, which is convenient for property maintenance.
文摘The problem of traffic congestion is a significant phenomenon that has had a substantial impact on the transportation system within the country. This phenomenon has given rise to numerous intricacies, particularly in instances where emergency situations occur at traffic light intersections that are consistently congested with a high volume of vehicles. This implementation of a traffic light controller system is designed with the intention of addressing this problem. The purpose of the system was to facilitate the operation of a 3-way traffic control light and provide priority to emergency vehicles using a Radio Frequency Identification (RFID) sensor and Reduced Instruction Set Computing (RISC) Architecture Based Microcontroller. This research work involved designing a system to mitigate the occurrence of accidents commonly observed at traffic light intersections, where vehicles often need to maneuver in order to make way for emergency vehicles following a designated route. The research effectively achieved the analysis, simulation and implementation of wireless communication devices for traffic light control. The implemented prototype utilizes RFID transmission, operates in conjunction with the sequential mode of traffic lights to alter the traffic light sequence accordingly and reverts the traffic lights back to their normal sequence after the emergency vehicle has passed the traffic lights.
基金ThispaperissupportedbyNationalNaturalScienceFoundation (No .5 9775 0 62 )
文摘The character of droplet transfer arc light sense signal is studied and validated in oscillating feed mode. And a novel mini photoelectric arc light sensor together with the controlling circuit used to automatically stabilize the signal range is developed. Further more the automatically identifying of the droplet transfer character signal from arc light is realized reliably.
文摘Based on the characteristics of line structured light sensor, a speedy method for the calibration was established. With the coplanar reference target, the spacial pose between camera and optical plane can be calibrated by using of the camera’s projective center and the light’s information in the camera’s image surface. Without striction to the movement of the coplanar reference target and assistant adjustment equipment, this calibration method can be implemented. This method has been used and decreased the cost of calibration equipment, simplified the calibration procedure, improved calibration efficiency. Using experiment, the sensor can attain relative accuracy about 0.5%, which indicates the rationality and effectivity of this method.
基金National Natural Science Foundation of China(60577005)Specialized Research Fund for the Doctoral Program of Higher Education Institute of MOE,China,to Harbin Engineering University
文摘A multiplexed white light interferometric fiber optic twin-sensor-array was designed to monitor the structural health of large buildings.In this sensing system,based on a Michelson interferometer,an optical path matching technique is used to demodulate each twin-sensor.Each twin-sensor-array consists of a 2×N sensing element linked by a 3 dB coupler.When one of the twin-sensor is used to measure strain,variations caused by temperature can be compensated for by referencing the other twin-sensor.The multiplexing capacity of the sensing scheme has been analyzed and experimental results with a 2×3 twin-sensor-array are given.
