Neurochip Based on Light-addressable Potentiometric Sensor

<正>A novel neurochip based on light addressable potentiometric sensor (LAPS) is designed.Using its light addressable characteristic.The problems of the limitations of restricted discrete active sites of current neurochips,such as microelectrode array and field effect transistor array can be settled easily.Based on the theoretical analysis of the interface between cells and LAPS,spontaneously discharges of hippocampal neurons induced by Mg~ 2+)-free media treatment were recorded by LAPS.The results demonstrate that this kind of neurochip has potential to monitor electrophysiology of cultured cells in a non-invasive way.

Neurochip based on light-addressable potentiometric sensor with wavelet transform de-noising

Neurochip based on light-addressable potentiometric sensor(LAPS),whose sensing elements are excitable cells,can monitor electrophysiological properties of cultured neuron networks with cellular signals well analyzed.Here we report a kind of neurochip with rat pheochromocytoma(PC12) cells hybrid with LAPS and a method of de-noising signals based on wavelet transform.Cells were cultured on LAPS for several days to form networks,and we then used LAPS system to detect the extracellular potentials with signals de-noised according to decomposition in the time-frequency space.The signal was decomposed into various scales,and coefficients were processed based on the properties of each layer.At last,signal was reconstructed based on the new coefficients.The results show that after de-noising,baseline drift is removed and signal-to-noise ratio is increased.It suggests that the neurochip of PC12 cells coupled to LAPS is stable and suitable for long-term and non-invasive measurement of cell electrophysiological properties with wavelet transform,taking advantage of its time-frequency localization analysis to reduce noise.

Research on the enhancement of signal-to-noise ratio of light-addressable potentiometric sensor by optical focusing

For enhancing the response of light-addressable potentiometric sensor(LAPS) and further improving its signal-to-noise ratio(SNR),an optical focusing method is adopted.Experimental research and theoretical analysis reveal that the magnitude of responsive signal is increased by optical focusing,and the SNR is improved remarkably.These research results indicate that the optical focusing is an effective approach for improving SNR of LAPS.

Detection of Chlorides in Pore Water of Cement Based Materials by Potentiometric Sensors

Free chloride ions dissolved in pore water of cement based materials initiate the corrosion of steel rebars used for reinforcement of concrete and thus pose serious constant danger to the safety of reinforced concrete structures.The in-situ monitoring of the content of chlorides in pore water with second order Ag/AgCl electrodes is an elegant approach which, however,suffers from poor stability of Ag/AgCl electrodes in highly alkaline environment like pore water.In this work the electrochemical stability of plain and stabilized electrodeposited Ag/AgCl sensors in extreme alkaline conditions are presented. The electrochemical processes were elucidated which govern the potentiometric response of Ag/AgCl sensors and determine their performance.The results have shown suitable stability and reproducibility of stabilized Ag/AgCl sensors over five months, which was sufficient for laboratory testing of chloride ingress into cement based materials.The embedded calibrated sensor elements were used to monitor the chloride uptake into cylindrical mortar specimen dipped into simulated pore water containing about 3 wt% of chloride ions.The velocity of diffusing front and the diffusion coefficient of chloride ions in pore water of cylindrical mortar sample were estimated from concentration-time profiles determined by chloride sensors positioned at defined positions in the sample wall.

A NOVEL BITTER DETECTION BIOSENSOR BASED ON LIGHT ADDRESSABLE POTENTIOMETRIC SENSOR

This paper presents a novel biosensor for bitter substance detection on the basis of light addressable potentiometric sensor(LAPS).Taste receptor cells(TRCs)were used as sensitive elements,which can respond to different bitter stimuli with extreme high sensitivity and speci-ficity.TRCs were isolated from the taste buds of rats and cultured on the surface of LAPS chip.Due to the unique advantages such as single-cell recording,light addressable capability,and noninvasiveness,LAPS chip was used as secondary transducer to monitor the responses of TRCs by recording extracelluar potential changes.The results indicate LAPS chip can effectively record the responses of TRCs to different bitter substances used in this study in a real-time manner for a long-term.In addition,by performing principal component analysis on the LAPS recording data,different bitter substances tested can be successfully discriminated.It is suggested this TRCs
LAPS hybrid biosensor could be a valuable tool for bitter substance detection.With further improvement and novel design,it has great potentials to be applied in both basic research and practical applications related to bitter taste detection.

All-Solid-State Screen-Printed Sensors for Potentiometric Calcium(II) Determinations in Environmental Samples

This paper describes preparation, characterization and electrochemical performance of novel planar miniaturized all-solid-state (ASS) screen-printed potentiometric sensors for the detection of Ca2+ ions in environmental samples. Screen-printed graphite-based ion-selective electrodes (ISEs) and screen-printed reference electrodes based on silver-containing pastes have been applied in a space saving manner on common ceramic substrates with small dimensions. Applications to environmental samples are shown by direct potentiometry and potentiometric titrations in real water samples. Conducting polymers (CPs) have been used as solid-contact materials and as intermediate layer between the polyvinyl chloride (PVC)-containing ion-selective membrane and the graphite-containing substrate. Different diamides have been incorporated into the PVC membrane. In the range from 10-4 mol/L to 10-1 mol/L, the ISEs show linear slopes of 27 mV/decade, which is close to the Nernstian response. Moreover, the ISEs have response times of 6 months. The novel potentiometric ASS sensors enable simple and exact Ca2+ determinations in real samples.

Enhanced Electrochemical and Physical Properties of Ag/AgCl Planar Reference Electrodesin Potentiometric Sensors by Graphite Oxide Layer

The most common reference electrode (RE) which is used in electrochemical measurements is the Ag/AgCl electrode. In this study, we present a novel solid-state Ag/AgCl planar electrode that was coated with a thin layer of Graphite Oxide (GO) as a protective layer. The Ag/AgCl planar electrode was fabricated by using the photolithography and lift-off method combined with the Ag[NH3]2Cl complex. The GO was produced by Hummer’s method and was deposited on top of the Ag/AgCl layer by drop-casting method. The layers of the fabricated reference electrode were characterized by micro Raman spectroscopy, Scanning Electron Microscopy (SEM) and Energy-dispersive X-ray spectroscopy (EDX). The responding time of the planar electrode was short and the signal was more stable in comparison to the plainAg/AgCl electrode. The Open Circuit Potential (OCP) measurement with the fabricated electrode as the RE and the platinum electrode as the working electrode in electrolyte solutions which had various pH values was performed with a linear response in pH ranges from pH 5 to pH 8 and the linear correlation coefficient (R^2 = 0.9899). Moreover, the coating of the GO layers also enhanced the durability of the modified electrode. The results showed that the modified Ag/AgCl electrode with a thin layer GO as the protective layer could be used as plana REs for the potentiometric sensors.

光寻址电位传感器的自相关检测方法

针对基于光寻址电位传感器的微流控芯片信噪比低的问题,提出了一种基于自相关检测的光寻址电位传感器信号提取方法。采用气液分离的方法在微流路内产生了体积约1μL的样品微滴,对比了光寻址电位传感器在方波激励与正弦波激励下检测微滴的I/V特性曲线,以及在相同条件下的I-f特性曲线。结果表明:采用自相关检测法,光寻址电位传感器具有53.8 mV/pH的灵敏度,其信噪比提高了7.020 6 dB。研究结果对于微型LAPS光电流信号高精度检测做出了有益探索,具有良好的应用前景。

A Novel Structure of LAPS Array for Cell-Based Biosensor

The light-addressable potentiometric sensor (LAPS) is a semiconductor-based cellular biosensor with an electrolyte-insulator-semiconductor (EIS) structure.By depositing biocompatible layers on the sensing surface for cell culture, it can be used to detect bioelectrical parameters of cells.The characteristic curve for photocurrent versus applied bias voltage to the system shows a current-voltage curve (Ⅰ-Ⅴcurve).This technique can be used to detect the action potential changes towards different drugs based on the bias voltage dependence of an optical current,and provides a dynamic system by scanning light beam at the very cell on the sensor device.The LAPS overcomes the limitation of recording sites,but high spatial resolution and sensitivity are also paramount.This paper discussed a novel structure of LAPS array for extracellular monitoring to decrease potential noise level.Both characteristics of active recording array areas and cell culture conditions are measured.

基于丝网印刷传感器的重金属现场快速检测仪研制

基于丝网印刷传感技术研制的现场快速重金属检测仪,采用微分电位溶出的原理,可应用于水样、污泥、食品、农产品中重金属铅的快速检测.该仪器具有体积小、便于携带、检测速度快、灵敏度高、操作简便且耗样量少等优点.对某地污泥中的铅进行含量测定,结果显示,测定结果的相对标准偏差RSD小于2%,回收率为98.49%~101.58%,具有良好的准确度和精密度.经过样品前处理后,单个样品检测时间约3 min.该仪器在现场痕量重金属检测方面具有良好的应用前景.

A non-labeled DNA biosensor based on light addressable potentiometric sensor modified with TiO_2 thin film

Titanium dioxide (TiO2) thin film was deposited on the surface of the light addressable potentiometric sensor (LAPS) to modify the sensor surface for the non-labeled detection of DNA molecules. To evaluate the effect of ultraviolet (UV) treatment on the silanization level of TiO2 thin film by 3-aminopropyltriethoxysilane (APTS),fluorescein isothiocyanate (FITC) was used to label the amine group on the end of APTS immobilized onto the TiO2 thin film. We found that,with UV irradiation,the silanization level of the irradiated area of the TiO2 film was improved compared with the non-irradiated area under well-controlled conditions. This result indicates that TiO2 can act as a coating material on the biosensor surface to improve the effect and efficiency of the covalent immobilization of biomolecules on the sensor surface. The artificially synthesized probe DNA molecules were covalently linked onto the surface of TiO2 film. The hybridization of probe DNA and target DNA was monitored by the recording of I-V curves that shift along the voltage axis during the process of reaction. A significant LAPS signal can be detected at 10 μmol/L of target DNA sample.

Topological Data Analysis of Potentiometric Multisensor Measurements in Treated Wastewater

In this study,a multisensor system consisting of 23 potentiometric sensors was applied for long-term online measurements in outlet flow of the water treatment plant.Within 1 month of continuous measurements,the data set of more than 295,000 observations was acquired.The processing of this dataset with conventional chemometric tools was cumbersome and not very informative.Topological data analysis(TDA)was recently suggested in chemometric literature to deal with large spectroscopic datasets.In this research,we explore the opportunities of TDA with respect to multisensor data with only 23 variables.It is shown that TDA allows for convenient data visualization,studying the evolution of water quality during the measurements and tracking the periodical structure in the data related to the water quality depending on the time of the day and the day of the week.TDA appears to be a valuable tool for multisensor data exploration.

汽车尾气用NO_x传感器

汽车尾气中的有害物主要有CO,HC,NOx,SOx以及一些微粒物质,给人类赖以生存的大气环境带来了严重的危害。汽车尾气用NOx传感器开发主要是为了解决NOx(包括NO,NO2)的排放问题,能够实现准确、快速地测定NOx含量,从而满足大气质量检测和环境保护的要求。目前,NOx气体传感器主要有半导体型、电位型和电流型。主要介绍了这3种汽车用NOx传感器的原理、结构及应用,并简要分析了其向微型化、集成化和智能化等方向发展的趋势。

分子印迹电位型传感器快速检测猪尿液中的克伦特罗

以盐酸克伦特罗为模板分子,采用沉淀聚合法合成了克伦特罗的分子印迹聚合物,并以其为离子载体,制得分子印迹聚合物膜克伦特罗离子选择性电极。在最优实验条件下,电极对克伦特罗阳离子的检出限可达7.0×10-8mol/L,线性范围为1.0×10-7～1.0×10-4mol/L,能斯特斜率为55.7 mV/decade。此电极具有优越的选择性、快速的响应时间以及良好的稳定性;已成功应用于实际猪尿样品中克伦特罗的测定,加标回收率为98%～107%,检测时间小于3 min。

平板式ZrO_2汽车氧传感器的结构、原理及研究进展

庞大的汽车氧传感器市场、严格的汽车尾气排放法规和国内相关技术的欠缺,使得研究和开发具有自主产权的、性能好、可靠性高的氧传感器产品显得尤为迫切和重要。目前,平板式ZrO2汽车氧传感器由于具有尺寸小、响应快、能耗低、易集成、在恶劣环境下工作稳定等优点,而成为汽车尾气控制的主流氧传感器。介绍了该传感器的结构、原理、国内外发展研究进展及发展趋势。

平板式二氧化锆氧传感器的研究进展

由于日益严格的排放法规,平板式二氧化锆氧传感器将成为汽车尾气控制应用的主流传感器。介绍了平板式氧传感器的制备工艺、结构和三种平板式氧传感器的工作原理并进行了对比。最后,展望了平板式氧传感器的发展前景。

一种新型生物化学图象传感器的研究

本文介绍了一种新型的光寻址生物和化学图象传感器的基本原理、结构及特点 ,并着重介绍了我们开发研制的生物化学图象传感器和有关的测试实验结果。完成了半导体硅器件的制作、生物化学敏感膜的制备、以激光器为主的光学扫描和机械定位系统以及基于微机系统的测量控制系统的设计制作。该传感器可以检测不同的生物和化学物质的静态及动态响应图象。实验结果表明 :该光寻址生物化学传感器系统具有较高灵敏度和分辨率以及较好的光寻址成象能力。利用该传感器可望开发出新一代的集成生物化学图象传感器。

基于微透镜阵列的多通道光寻址电位传感器研究

为了提高化学和生物图像传感器的成像速度和简化成像系统,提出了一种基于微透镜阵列的多通道光寻址电位传感器.利用微透镜阵列把单束激光转化成点阵,通过多频率斩波器,得到不同频率的光点阵列用作光寻址电位传感器的光源,从而实现多点同时检测.实验结果表明该系统具有良好的频域分辨率,通过数字补偿技术可获得各个检测点的电流电压特性曲线,证明可实现多点同时检测.该系统可以用于光寻址电位传感器和扫描光感阻抗传感器的快速成像,以及多通道化学生物图像检测.

基于氧硫功能团载体的铅离子电位传感器的研究与进展

系统总结归纳了基于氧硫功能团载体的铅离子电位传感器,具体涉及到蒽醌、芳羧酸、羧酸酯、磷酸酯和含酰胺、硫酰胺大环化合物等。蒽醌类载体普遍具有较快的铅离子响应速度,响应时间为2 s。含硫芳香羧酸对铅离子的传感普遍较为敏感,构建不对称膜后,探检出限改善至6.0×10-10mol/L。磷酸酯则具有很强的抗干扰能力,大部分金属离子选择系数logK均小于-3.0。而含酰胺基团的大环化合物载体已构建成微型传感器,从而可探测受限于3μL测量池的样品,检出限达2.7×10-9mol/L。

一种新型汞离子选择薄膜传感器

采用脉冲激光沉积技术,在光寻址电位传感器表面上沉积了对二价汞离子敏感的薄膜,制备了一种新型汞离子选择薄膜传感器,靶材成分为HgAgIS,基底为p型单晶硅片,金属接触层为Cr/Au。该薄膜传感器在3星期内显示了良好的重复性和稳定性;检出限为3×10-6mol/L;响应时间小于2min,适用pH范围小于2。对干扰离子和迟滞效应等也进行了研究。该传感器具有测量快速灵活、所需样品少、动态范围宽等特点,因为把电位信号转化为对光激发的交流电流信号进行测量,所以提高了灵敏度。同时也证明了脉冲激光沉积是适合制备薄膜传感器的一种新技术。