Impact of wetting-drying cycles and acidic conditions on the soil aggregate stability of yellow‒brown soil

Soil aggregate is the basic structural unit of soil,which is the foundation for supporting ecosystem functions,while its composition and stability is significantly affected by the external environment.This study was conducted to explore the effect of external environment(wetting-drying cycles and acidic conditions)on the soil aggregate distribution and stability and identify the key soil physicochemical factors that affect the soil aggregate stability.The yellow‒brown soil from the Three Gorges Reservoir area(TGRA)was used,and 8 wetting-drying conditions(0,1,2,3,4,5,10 and 15 cycles)were simulated under 4 acidic conditions(pH=3,4,5 and 7).The particle size distribution and soil aggregate stability were determined by wet sieving method,the contribution of environmental factors(acid condition,wetting-drying cycle and their combined action)to the soil aggregate stability was clarified and the key soil physicochemical factors that affect the soil aggregate stability under wetting-drying cycles and acidic conditions were determined by using the Pearson’s correlation analysis,Partial least squares path modeling(PLS‒PM)and multiple linear regression analysis.The results indicate that wetting-drying cycles and acidic conditions have significant effects on the stability of soil aggregates,the soil aggregate stability gradually decreases with increasing number of wetting-drying cycles and it obviously decreases with the increase of acidity.Moreover,the combination of wetting-drying cycles and acidic conditions aggravate the reduction in the soil aggregate stability.The wetting-drying cycles,acidic conditions and their combined effect imposes significant impact on the soil aggregate stability,and the wetting-drying cycles exert the greatest influence.The soil aggregate stability is significantly correlated with the pH,Ca^(2+),Mg^(2+),maximum disintegration index(MDI)and soil bulk density(SBD).The PLS‒PM and multiple linear regression analysis further reveal that the soil aggregate stability is primarily influenced by SBD,Ca^(2+),and MDI.These results offer a scientific basis for understanding the soil aggregate breakdown mechanism and are helpful for clarifying the coupled effect of wetting-drying cycles and acid rain on terrestrial ecosystems in the TGRA.

Calcium carbonate promotes the formation and stability of soil macroaggregates in mining areas of China

We studied changes in the concentrations of aggregate-cementing agents after different reclamation times and with different fertilization regimes,as well as the formation mechanism of aggregates in reclaimed soil,to provide a theoretical basis for rapid reclamation of soil fertility in the subsidence area of coal mines in Shanxi Province,China.In this study,soil samples of 0–20 cm depth were collected from four fertilization treatments of a longterm experiment started in 2008:no fertilizer (CK),inorganic fertilizer (NPK),chicken manure compost (M),and50%inorganic fertilizer plus 50%chicken manure compost (MNPK).The concentrations of cementing agents and changes in soil aggregate size distribution and stability were analysed.The results showed that the formation of>2 mm aggregates,the aggregate mean weight diameter (MWD),and the proportion of>0.25 mm water-stable aggregates (WR_(0.25)) increased significantly after 6 and 11 years of reclamation.The concentration of organic cementing agents tended to increase with reclamation time,whereas free iron oxide (Fed) and free aluminium oxide(Ald) concentrations initially increased but then decreased.In general,the MNPK treatment signi?cantly increased the concentrations of organic cementing agents and CaCO_(3),and CaCO_(3) increased by 60.4%at 11 years after reclamation.Additionally,CaCO_(3) had the greatest effect on the stability of aggregates,promoting the formation of>0.25 mm aggregates and accounting for 54.4%of the variance in the proportion and stability of the aggregates.It was concluded that long-term reclamation is bene?cial for improving soil structure.The MNPK treatment was the most effective measure for increasing maize grain yield and concentration of organic cementing agents and CaCO_(3).

Human islet amyloid polypeptide oligomers stabilized and probed by MAS NMR

The capture and characterization of oligomers are extremely important in the studies of amyloid aggregation of proteins and peptides.Oligomers are critical intermediates that can impact the structures of amyloid fibrils.Moreover,it is widely accepted that oligomers are the most toxic species along the aggregation pathway[1e4].The studies of oligomers are believed to shed light on the molecular mechanism of amyloid fibrillation and probably the medical clues for related diseases.In vitro investigations of amyloid oligomers are challenging due to their transient and polymorphic nature[5].This is particularly evident in the case of human type-2 diabetes-associated islet amyloid polypeptide(hIAPP),which tends to rapidly form polymorphic fibrils within minutes[6].Notably,hIAPP demonstrates a higher propensity for rapid aggregation compared to other amyloid proteins such as a-synuclein[7].

AGGREGATE SPECIAL FUNCTIONS TO APPROXIMATE PERMUTING TRI-HOMOMORPHISMS AND PERMUTING TRI-DERIVATIONS ASSOCIATED WITH A TRI-ADDITIVEψ-FUNCTIONAL INEQUALITY IN BANACH ALGEBRAS

In this paper,we define a new class of control functions through aggregate special functions.These class of control functions help us to stabilize and approximate a tri-additiveψ-functional inequality to get a better estimation for permuting tri-homomorphisms and permuting tri-derivations in unital C*-algebras and Banach algebras by the vector-valued alternative fixed point theorem.

The effects of co-utilizing green manure and rice straw on soil aggregates and soil carbon stability in a paddy soil in southern China

The co-utilization of green manure (GM) and rice straw (RS) in paddy fields has been widely applied as an effective practice in southern China.However,its effects on soil aggregate and soil organic carbon (SOC) stability remain unclear.In the present study,the effect of GM,RS,and co-utilization of GM and RS on particle size distribution of soil aggregates and SOC density fractions were measured in a field experiment.The experiment included six treatments,i.e.,winter fallow (WF) without RS return (Ctrl),WF with 50%RS return (1/2RS),WF with 100%RS return (RS),GM without RS return (GM),GM with 50%RS return (GM1/2RS) and GM with 100%RS return (GMRS).The results showed that the proportion of small macro-aggregates (0.25–2 mm) and the mean weight diameter (MWD) of aggregates in the GMRS treatment was greater (by 18.9 and 3.41%,respectively) than in the RS treatment,while the proportion of silt+clay particles (<0.053 mm) was lower (by 14.4%).The concentration of SOC in microaggregates (0.053–0.25 mm)and silt+clay particles was higher in the GMRS treatment than in GM and RS treatments individually.The concentration and proportion of free light organic carbon (fLOC) in aggregates of various particle sizes and bulk soil was greater in the GMRS treatment than the RS treatment,whereas the concentration and proportion of mineral-associated organic carbon in small macroaggregates,microaggregates,and bulk was lower in the GMRS treatment than in the RS treatment.The proportion of intra-aggregate particulate organic carbon (iPOC) was greater in the GMRS treatment than in GM treatment.The GMRS treatment had strong positive effects on iPOC in small macroaggregates,suggesting that SOC was transferred from fLOC to iPOC.In conclusion,co-utilizing green manure and rice straw cultivated the SOC pool by increasing the concentration of fLOC and improved soil carbon stability by promoting the sequestration of organic carbon in iPOC as a form of physical protection.

Preliminary Study on the Effect of Different Ecological Cultivation Modes on the Water Stability of Soil Aggregates in Rubber Based Agroforestry Systems

Rubber trees (Hevea brasiliensis Müll. Arg.) have been commercially cultivated for a century and a half in Asia, particularly in China, and they constitute a common element of plantation ecosystems in tropical regions. Soil health is fundamental to the sustainable development of rubber plantations. The objective of the study is to explore the influence of different complex ecological cultivation modes on the stability of soil aggregates in rubber based agroforestry systems. In this study, the ecological cultivation mode of rubber—Alpinia oxyphylla plantation, the ecological cultivation mode of rubber—Phrynium hainanense plantations, the ecological cultivation mode of rubber—Homalium ceylanicum plantations and monoculture rubber plantations were selected, and the particle size distribution of soil aggregates and their water stability characteristics were analyzed. The soil depth of 0 - 20 cm and 20 - 40 cm was collected for four cultivation modes. Soil was divided into 6 particle levels > 20 cm. soil was divided into 6 particle levels > 5 mm, 2 - 5 mm, 1 - 2 mm, 0.5 - 1 mm, 0.25 - 0.5 mm, and 0.053 - 0.25 mm according to the wet sieve method. The particle size proportion and water stability of soil aggregates were determined by the wet sieve method. The particle size proportion and water stability of soil aggregates under different ecological cultivation modes were analyzed. The results showed that under different ecological cultivation modes in the shallow soil layer (0 - 20 cm), the rubber—Alpinia oxyphylla plantation and the rubber—Phrynium hainanense plantation promoted the development of dominant soil aggregates towards larger size classes, whereas the situation is the opposite for rubber—Homalium ceylanicum plantation. In soil layer (20 - 40 cm), the ecological cultivation mode of rubber—Phrynium hainanense plantation developed the dominant radial level of soil aggregates to the diameter level of large aggregates. Rubber—Alpinia oxyphylla plantation and rubber—Homalium ceylanicum plantation, three indicators, including the water-stable aggregate content R0.25 (>0.25 mm water-stable aggregates), mean weight diameter (MWD), and geometric mean diameter (GMD), were all lower than those in the rubber monoculture mode. However, in the rubber—Phrynium hainanense plantation, the water-stable aggregate content R0.25, mean weight diameter, and geometric mean diameter were higher than in the rubber monoculture mode, although these differences did not reach statistical significance.

THE AVERAGE AGGREGATE NUMBER OF ELECTROSTATICALLY STABILIZED AGGREGATE(ESAg)

The average aggregate number(N)of electrostatically stabilized aggregate(ESAg)composed of oppositely-charged long-chain molecules,i.e., sodium ω-[α-(nathphyl)ethoxyl]undecanoate(FP^-)and cetyltrimethyl ammonium chloride(CTAC),in aqueous solution at 25℃ has been measured to be 11 to 16 in the CTAC-concentration range of 11×10^(-5) M to 30×10^(-5) M at a fixed FP- concentration of 1.0×10^(-5)M by the photon counting method.

Electrostatically Stabilized Aggregate (ESAg). Ⅰ. Evidence Derived from the Coaggregating Behavior of an Anionic Kinetic Probe (P16^-) with Surfactants

A newly proposed ESAg concept is verified by the coaggregation behavior of an anionic kinetic probe （P16;） with various surfactants.

ELECTROSTATICALLY STABILIZED AGGREGATE (ESAg) Ⅱ.EVIDENCE DERIVED FROM THE COAGGREGATING BEHAVIOR OF A CATIONIC KINETIC PROBE (P16^+) WITH SURFACTANTS

The coaggregating behavior of the cationic kinetic probe P16;with different types of surfactants are in complete agreement with predictions based on the newly proposed ESAg concept.

Soil Aggregate Stability and Aggregate-Associated Carbon Under Different Tillage Systems in the North China Plain

The inlfuences of tillage systems on soil carbon （C） stocks have been studied extensively, but the distribution of soil C within aggregate fractions is not well understood. The objective of this study was to determine the inlfuences of various tillage systems on soil aggregation and aggregate-associated C under wheat （Triticum aestivum L.） and corn （Zea mays L.） double cropping systems in the North China Plain. The experiment was established in 2001, including four treatments：moldboard plow （MP） with residue （MP＋R） and without residue （MP-R）, rotary tillage with residue （RT）, and no-till with residue （NT）. In 2007 soil samples were collected from the 0-5, 5-10, and 10-20 cm depths, and were separated into four aggregate-size classes （〉2 000, 250-2 000, 53-250, and〈53 μm） by wet-sieving method. Aggregate-associated C was determined, and the relationships between total soil C concentration and aggregation-size fractions were examined. The results showed that NT and RT treatments signiifcantly increased the proportion of macroaggregate fractions （〉2 000 and 250-2 000 μm） compared with the MP-R and MP＋R treatments. Averaged across all depths, mean weight diameters of aggregates （MWD） in NT and RT were 47 and 20% higher than that in MP＋R. The concentration of bulk soil organic C was positively correlated with MWD （r=0.98; P=0.024） and macroaggregate fraction （r=0.96; P=0.036） in the 0-5 cm depth. In the 0-20 cm depth, comparing with MP＋R, total C occluded in the〉2 000 μm fraction was increased by 9 and 6%under NT and RT, respectively. We conclude that adoption of conservation tillage system, especially no-till, can increase soil macro-aggregation and total C accumulation in macroaggregates, which may improve soil C sequestration in the intensive agricultural region of the North China Plain.

Formation and Water Stability of Aggregates in Red Soils as Affected by Organic Matter

The water stability of aggregates in various size classes separated from 18 samples of red soils under different managements, and the mechanisms responsible for the formation of water-stable soil aggregates were studied. The results showed that the water stability of soil aggregates declined with increasing size, especially for the low organic matter soils. Organic matter plays a key role in the formation of water-stable soil aggregates. The larger the soil aggregate size, the greater the impact of organic matter on the water stability of soil aggregates. Removal of organic matter markedly disintegrated the large water-stable aggregates (> 2.0 mm) and increased the small ones (< 0.25-0.smm) to some extent, whereas removal of free iron(aluminium) oxides considerably destroyed aggregates of all sizes, especially the < 0.25-0.5 mm classes. The contents of organic matter in water-stable aggregates increased with aggregate sizes. It is concluded from this study that small water-stable aggregates (< 0.25-0.5 mm) were chiefly cemented by Fe and Al oxides whilst the large ones (> 2.0 mm) were mainly glued up by organic matter. Both free oxides and organic matter contribute to the formation and water stability of aggregates in red soils.

Methods for Improving Volume Stability of Steel Slag as Fine Aggregate

Suitable methods for enhancing the volume stability of steel slag utilized as fine aggregate were determined. The effects of steam treatment at 100 ℃ and autoclave treatment under 2.0 MPa on the soundness of steel slag sand were investigated by means of powder ratio, linear expansion, compressive and flexural strength. DTA, EDX, XRD and ethylene glycol methods were employed to analyze both the treated slags and susceptible expansion grains. Experimental results indicate that powder ratio, content of free lime and rate of linear expansion can express the improvement in volume stability of different treated methods. Steam treatment process cannot ultimately prevent specimens from cracking and decrease of strength, but mortar made from autoclave treated slag keeps integration subjected to hot water of 80℃ until 28 d and its strength do not show significant decrement. The hydration of over-burn free lime and periclase phase are the main cause for the disintegration or crack of untreated and steam treated steel slag＇s specimens. Autoclave treatment process is more effective than steam treatment process on enhancement of volume stability of steel slag.

Effect of Cultivation on Soil Organic Matter and Aggregate Stability

Agricultural sustainability relates directly to maintaining or enhancing soil quality. Soil quality studies in Canada during the 1980 s showed that loss of soil organic matter (SOM) and soil aggregate stability was standard features of non-sustainable land management in agroecosystems. In this study total soil organic carbon (SOC), particulate organic matter (POM), POM-C as a percentage of total SOC, and aggregate stability were determined for three cultivated fields and three adjacent grassland fields to a…

Aggregate stability and associated C and N in a silty loam soil as affected by organic material inputs

To make recycling utilization of organic materials produced in various agricultural systems, five kinds of organic materials were applied in a field test, including crop straw （CS）, biogas residue （BR）, mushroom residue （MR）, wine residue （WR）, pig manure （PM）, with a mineral fertilizer （CF） and a no-fertilizer （CK） treatment as a control. Our objectives were： i） to quantify the effects of organic materials on soil C and N accumulation; ii） to evaluate the effects of organic materials on soil aggregate stability, along with the total organic carbon （TOC）, and N in different aggregate fractions; and iii） to assess the relationships among the organic material components, soil C and N, and C, N in aggregate fractions. The trial was conducted in Wuqiao County, Hebei Province, China. The organic materials were incorporated at an equal rate of C, and combined with a mineral fertilizer in amounts of 150 kg N ha^-1, 26 kg P ha^-1 and 124 kg K ha-1 respectively during each crop season of a wheat-maize rotation system. The inputted C quantity of each organic material treatment was equivalent to the total amount of C contained in the crop straw harvested in CS treatement in the previous season. TOC, N, water-stable aggregates, and aggregate-associated TOC and N were investigated. The results showed that organic material incorporation increased soil aggregation and stabilization. On average, the soil macroaggregate proportion increased by 14%, the microaggregate proportion increased by 3%, and mean-weight diameter （MWD） increased by 20%. TOC content followed the order of PM〉WR〉MR〉BR〉CS〉CK〉CF; N content followed the order WR〉PM〉MR〉BR〉CS〉CF〉CK. No significant correlation was found between TOC, N, and the quality of organic material. Soil silt and clay particles contained the largest part of TOC, whereas the small macroaggregate fraction was the most sensitive to organic materials. Our results indicate that PM and WR exerted better effects on soil C and N accumulation, followed by MR and BR, suggesting that organic materials from ex situ farmland could promote soil quality more as compared to straw returned in situ.

Effect of phosphogypsum on saline-alkalinity and aggregate stability of bauxite residue

A column experiment was conducted to investigate the effect of phosphogypsum(PG)on the salinealkalinity,and aggregate stability of bauxite residue.Results showed that:with increasing leaching time,the concentrations of saline−alkali ions decreased while the SO_(4)^(2-)concentration increased in bauxite residue leachate;compared with CK(control group)treatment,pH,electric conductivity(EC),exchangeable sodium percentage(ESP),sodium absorption ratio(SAR),and exchangeable Na+content of bauxite residue were reduced following PG treatment;average particle sizes in aggregates following CK and PG treatments were determined to be 155 and 193 nm,respectively.SR-μCT test results also confirmed that bauxite residue following PG treatment acquired larger aggregates and larger pore diameter.These results indicate that the PG treatment could significantly modulate the saline-alkalinity,and simultaneously enhance aggregate stability of bauxite residue,which provides a facile approach to reclaim bauxite residue disposal areas.

An evaluation on using soil aggregate stability as the indicator of interrill erodibility

Aggregate stability is a very important predictor of soil structure and strength, which influences soil erodibility. Several aggregate stability indices were selected erodibility of four soil properties from temperate for estimating interrill types with contrasting and subtropical regions of China. This study was conducted to investigate how closely the soil interrill erodibility factor in the Water Erosion Prediction Project （WEPP） model relates to soil aggregate stability. The mass fractal dimension （FD）, geometric mean diameter （GMD）, mean weight diameter （MWD）, and aggregate stability index （ASI） of soil aggregates were calculated. A rainfall simulator with a drainable flume （3.0 m long × 1.0 m wide × 0.5 m deep） was used at four slope gradients （5°,10 °,15° and 20°）, and four rainfall intensities （0.6, 1.1, 1.7 and 2.5 mm/min）. Results indicated that the interriU erodibility （Ki） values were significantly correlated to the indices of ASI, MWD, GMD, and FD computed from the aggregate wet-sieve data. The Kihad a strong positive correlation with FD, as well as a strong negative correlation with ASI, GMD, and MWD. Soils with a higher aggregate stability and lower fractal dimension have smaller Ki values. Stable soils were characterized by a high percentage of large aggregates and the erodible soils by a high percentage of smaller aggregates. The correlation coefficients of Ki with ASI and GMD were greater than those with FD and MWD, implying that both the ASI and GMD may be better alternative parameters for empirically predicting the soil Ki factor. ASI and GMD are more reasonable in interrill soil erodibility estimation, compared with Ki calculation in original WEPP model equation. Results demonstrate the validation of soil aggregation characterization as an appropriate indicator of soil susceptibility to erosion in contrasting soil types in China.

Effect of substrate amendment on alkaline minerals and aggregate stability in bauxite residue

Bauxite residue is an alkaline waste material in the process of alumina production due to its characteristics of higher salinity and alkalinity,which results in environmental issues and extremely restricts the sustainable development of alumina industries.In this work,we conduct a column experiment to study the effects of two amendments on aggregate stability and variations in alkaline minerals of bauxite residue.The two amendments are phosphogypsum(PG)and phosphogypsum and vermicompost(PVC).The dominant fraction in aggregate is 1–0.25 mm in diameter on the surface,which takes up 39.34%,39.38%,and 44.51%for CK,PG,and PVC,respectively.Additions of PG and PVC decreased pH,EC,ESP,exchangeable Na^+concentration and the percentage of alkaline minerals,and then increased exchangeable Ca^2+concentration in bauxite residue.There was significant positive correlation between pH and exchangeable Na^+concentration,the percentage of cancrinite,tricalcium aluminate and calcite;while negative correlation was found in pH value versus exchangeable Ca^2+concentration.Theses findings confirmed that additions of phosphogypsum and vermicompost have a stimulative effect on aggregate stability in bauxite residue.In particular,amendment neutralization(phosphogypsum+vermicompost)in column represents an advantage for large-scale simulation of vegetation rehabilitate in bauxite residue disposal areas.

Assessment of soil erodibility and aggregate stability for different parts of a forest road

We measured erodibility and mean weight diameter （MWD） of soil aggregates in different parts of a forest road. Samples of topsoil were collected from cutslope, fillslope, road surface and forest ground to assess the texture, bulk density, moisture, CaCO3 and organic matter. Soil aggregate stability was determined by wet sieving. Soil erodibility on the road surface was 2.3 and 1.3 times higher than on the fillslope and cutslope, respectively. The forest soil had the lowest erodibility. Aggregate stability of cutslope and road surface were low and very low, respectively. There was a significant negative relationship between cutslope erodibility with CaCO3 and sand content. Cutslope erodibility increased with increasing silt, clay and moisture content. On fillslopes, MWD increased with in-creasing rock fragment cover, plant cover, litter cover, organic matter and sand. There was a strong negative correlation between fillslope erodibility and organic matter, sand and MWD. There was no significant difference between erodibility of bare soil and soils beneathRubus hyrcanusL. and Philonotis marchica （Hedw.） Brid.

Aggregate binding agents improve soil aggregate stability in Robinia pseudoacacia forests along a climatic gradient on the Loess Plateau,China

The distribution of binding agents(i.e.,soil organic carbon(SOC)and glomalin-related soil protein(GRSP))in soil aggregates was influenced by many factors,such as plant characteristics and soil properties.However,how these factors affect binding agents and soil aggregate stability along a climatic gradient remained unclear.We selected the Robinia pseudoacacia L.forests from semi-arid to semi-humid of the Loess Plateau,China to analyze the plant biomass,soil physical-chemical properties,SOC and GRSP distribution in different sized soil aggregates.We found that from semi-arid to semi-humid forests:(1)the proportion of macro-aggregates(>0.250 mm)significantly increased(P<0.05),whereas those of micro-aggregates(0.250–0.053 mm)and fine materials(<0.053 mm)decreased and soil aggregate stability was increased;(2)the contents of SOC and GRSP in macro-aggregates and micro-aggregates significantly increased,and those in fine materials decreased;(3)the contribution of SOC to soil aggregate stability was greater than those of total GRSP and easily extractable GRSP;(4)soil properties had greater influence on binding agents than plant biomass;and(5)soil aggregate stability was enhanced by increasing the contents of SOC and GRSP in macro-aggregates and soil property was the important part during this process.Climate change from semi-arid to semi-humid forests is important factor for soil structure formation because of its positive effect on soil aggregates.

Conversion of pure Chinese fir plantation to multi-layered mixed plantation enhances the soil aggregate stability by regulating microbial communities in subtropical China

Background:Soil aggregates are the basic units of soil structure,and their stability is a key indicator of soil quality and capacity to support ecosystem functions.The impacts of various environmental factors on soil aggregates have been widely studied.However,there remains elusive knowledge on the synergistic effects of changing forest stand structure on soil aggregate stability(SAS),particularly in subtropical China where soil erosion remains a critical issue.Methods:We investigated variations in the components of soil humus(HS),including humic acids(HAs),fulvic acids(FAs),and humins(HMs),under pure Chinese fir(Cunninghamia lanceolata)plantation(PP)and multilayered mixed plantation(MP)comprising C.lanceolata,Castanopsis hystrix,and Michelia hedyosperma.The state of soil aggregate stability,was determined by three separate methods,i.e.,dry-sieving,wet-sieving,and the Le Bissonnais.High-throughput sequencing was used to determine the diversity and composition of microbial communities under PP and MP.We then built partial least squares path models(PLS-PM)for assessing the responses of SAS to the variations in soil microorganisms and HS components.Results:The MP stands had significantly greater SAS(P<0.05),higher content of HAs and more rapid organic matter humification within aggregates,than the PP stands.High-throughput sequencing confirmed that the Pielou andα-diversity index values(Chao1 and Shannon)for fungi were all significantly higher under MP than under PP,while no marked difference was found in bacterialα-diversity between the two plantation types.Moreover,there were markedly greater abundance of three bacterial phyla(Verrucomicrobia,Chloroflexi,and Gemmatimonadetes)and three fungal phyla(Ascomycota,Kickxellomycota,and Glomeromycota),and significantly less abundance of two bacterial phyla(Planctomycetes and Firmicutes)and four fungal phyla(Basidiomycota,Mortierellomycota,Mucoromycota,and Rozellomycota)under MP than under PP.The Chloroflexi and Ascomycota phyla appeared to be the primary drivers of soil aggregate distribution.Our findings revealed that the promotion of SAS under MP was mainly driven by increased soil organic matter(SOM)content,which altered bacterial communities and enhanced fungal diversity,thereby increasing HAs content and the rate of organic matter humification.Conclusions:Considering the combined effects of enhanced soil quality,productivity,and relevant economic costs,introducing broadleaved tree species into Chinese fir plantations can be an effective strategy for stabilizing soil structure against erosion in subtropical China.Our study elucidated the controls on variations of SAS in Chinese fir-dominated plantations and demonstrated the benefit of converting pure Chinese fir plantation to multi-layered mixed plantations in increasing soil structural stability and improving site quality.