Identification of QTL for kernel number-related traits in a rice chromosome segment substitution line and fine mapping of qSP1

A chromosome segment substitution line (CSSL) is a powerful tool for combining quantitative trait locus (QTL) mapping with the pyramiding of desirable alleles. The rice CSSL Z1364 with increased kernel number was identified in a BC3F8 population derived from a cross of Nipponbare as the recipient with Xihui 18 as the donor parent. Z1364 carried three substitution segments distributed on chromosomes 1, 6, and 8. The mean substitution length was 1.19 Mb. Of 17 QTL identified on the substitution segments, qSP1 for spikelets per panicle, qSSD1 for seed-set density, and qNSB1 for number of secondary branches explained respectively 57.34%, 87.7%, and 49.44% of the corresponding phenotypic variance and were all linked to RM6777. Chi-square analysis showed that the increased kernel number in Z1364 was inherited recessively by a single gene. By fine mapping, qSP1 was delimited to a 50-kb region on the short arm of chromosome 1. Based on DNA sequence, a previously uncharacterized rice homolog of Arabidopsis thaliana AT4G32551 was identified as a candidate gene for qSP1 in which mutation increases the number of spikelets and kernels in Z1364. qSP1 was expressed in all tissues, but particularly in 1-cm panicles. The expression levels of OsMADS22, GN1A, and DST were upregulated and those of LAX2, GNP1, and GHD7 were downregulated in Nipponbare. These results provide a foundation for functional research on qSP1.

Determination of the Number of SSR Alleles Necessary for the Analysis of Genetic Relationships Between Maize Inbred Lines

The amount of molecular marker information has considerable impact on the results of studies of crop germplasm genetic relationships in crop. The number of alleles required to reveal genetic relationship in maize inbred lines is a theoretical issue that needs to be addressed. In this study, 112 pairs of SSR （simple sequence repeat） primers and 97 maize inbred lines were selected to study the relationship between the number of inbred lines and the number of SSR primers and alleles required for a stable cluster. The results showed that the number of SSR primers is not tightly associated with the stability of the cluster analysis results, while an increase in the number of alleles can significantly improve the stability of cluster analysis results. The number of inbred lines （X） is significantly associated with the number of alleles required for stable cluster analysis （Y）, and the regression equation is Y- 600.8xe（-15.9/x）. This equation can be used to calculate the number of SSR alleles required for a genetic relationship study of maize inbred lines. These results provide a reference for determining of SSR alleles number in genetic relationship analysis of maize inbred line and other crop germplasm.

Studies on the Developmental Genetics of Tiller Number in Three-line Indica Hybrid Rice

Following NCI design, the developmental genetic behavior of tiller number (TN) in three-line indica hybrid rice was studied using additive-dominance developmental genetic models and the corresponding statistical methods. The results showed that dominance effects were predominant for TN. The expression of those additive effects were affected by environment and genotype interaction, but the expression of dominance effects were not affected. Heterosis was the strongest in the middle developmental periods of TN. Additive effects and dominance effects were selectively expressed throughout in the entire tillering developmental stage. Analysis of genetic correlation between TN at different stages and the productive panicles indicated that a close correlation appeared earlier in the populations with higher heterosis than in those with less heterosis. Utilization of heterosis at the middle tillering stage might enhance the final biomass but reduce the percentage of productive panicles.

Characterization of a new hexaploid triticale 6D(6A) substitution line with increased grain weight and decreased spikelet number

Hexaploid triticale(×Triticosecale,AABBRR)is an important forage crop and a promising energy plant.Transferring D-genome chromosomes or segments from common wheat(Triticum aestivum)into hexaploid triticale is attractive in improving its economically important traits.Here,a hexaploid triticale 6D(6A)substitution line Lin 456 derived from the cross between the octoploid triticale line H400 and the hexaploid wheat Lin 56 was identified and analyzed by genomic in situ hybridization(GISH),fluorescence in situ hybridization(FISH),and molecular markers.The GISH analysis showed that Lin 456 is a hexaploid triticalewith 14 rye(Secale cereale)chromosomes and 28 wheat chromosomes,whereas non-denaturing fluorescence in situ hybridization(ND-FISH)and molecular marker analysis revealed that it is a 6D(6A)substitution line.In contrast to previous studies,the signal of Oligo-pSc119.2 was observed at the distal end of 6DL in Lin 456.The wheat chromosome 6D was associatedwith increased grain weight and decreased spikelet number using the genotypic data combined with the phenotypes of the F2 population in the three environments.The thousand-grain weight and grain width in the substitution individuals were significantly higher than those in the non-substitution individuals in the F2 population across the three environments.We propose that the hexaploid triticale 6D(6A)substitution line Lin 456 can be a valuable and promising donor stock for genetic improvement during triticale breeding.

Unconditional and Conditional QTL Mapping for Tiller Numbers at Various Stages with Single Segment Substitution Lines in Rice(Oryza sativa L.)

Tiller is one of the most important agronomic traits which influences quantity and quality of effective panicles and finally influences yield in rice. It is important to understand ＂static＂ and ＂dynamic＂ information of the QTLs for tillers in rice. This work was the first time to simultaneously map unconditional and conditional QTLs for tiller numbers at various stages by using single segment substitution lines in rice. Fourteen QTLs for tiller number, distributing on the corresponding substitution segments of chromosomes 1, 2, 3, 4, 6, 7 and 8 were detected. Both the number and the effect of the QTLs for tiller number were various at different stages, from 6 to 9 in the number and from 1.49 to 3.49 in the effect, respectively. Tiller number QTLs expressed in a time order, mainly detected at three stages of 0-7 d, 14-21 d and 35-42 d after transplanting with 6 positive, 9 random and 6 negative expressing QTLs, respectively. Each of the QTLs expressed one time at least during the whole duration of rice. The tiller number at a specific stage was determined by sum of QTL effects estimated by the unconditional method, while the increasing or decreasing number in a given time interval was controlled by the total of QTL effects estimated by the conditional method. These results demonstrated that it is highly effective and accurate for mapping of the QTLs by using single segment substitution lines and the conditional analysis methodology.

The Difference of the Copy Number Variation and Loss of Heterozygosity of Human Lung Large Cell Cancer Cell Line with Different Metastatic Potential

Background and Objective It has been proven that copy number gain/or loss (copy number variation CNV) in uences gene expression and result in phenotypic variation by

Topological Structure of Disclination Lines in 2-Dimensional Liquid Crystals

Using mapping method and topological current theory, the topological structure of disclination lines in 2 dimensional liquid crystals is studied. By introducing the strength density and the topological current of many disclination lines, it is pointed out that the disclination lines are determined by the singulaities of the director field, and topologically quantized by the Hopf indices and Brouwer degrees. Due to the equivalence in physics of the director fields n (x) and n (x) , the Hopf indices can be integers or half integers, representing a generalization of our previous studies of integer Hopf indices.

Modeling Density and Anisotropy of Energetic Electrons Along Magnetic Field Lines

The electromagnetic wave growth or damping depends basically on the number density and anisotropy of energetic particles as the resonant interaction takes place between the particles and waves in the magnetosphere. The variance of both the number density and anisotropy along the magnetic field line is evaluated systematically by modeling four typically prescribed distribution functions. It is shown that in the case of ＂the positive anisotropy＂ （namely, the perpendicular temperature T⊥ exceeds the parallel temperature T｜｜）, the number density of energetic electrons always decreases with the magnetic latitude for a regular increasing magnetic field and the maximum wave growth is therefore generally confined to the equator where the resonant energy is minimum, and the number density is the largest. However, the ＂loss-cone＂ anisotropy of the electrons with a ＂pancake＂ distribution or kappa distribution keeps invariant or nearly invariant, whereas the ＂temperature＂ anisotropy with a pure bi-Maxwellian distribution or Ashour-Abdalla and Kennel＇s distributions decreases with the magnetic latitude. The results may provide a useful approach to evaluating the number density and anisotropy of the energetic electrons at latitudes where the observation information is not available.

Comparison of QTL for Grain Number per Panicle in Three Populations Sharing 3 Parents and Fine Mapping of Gn1c

Complex traits, such as yield components, are inherited in a quantitative manner and typically controlled by quantitative trait loci (QTL). Grain number per panicle (GN) is an important component of yield in rice and

基于ST-number算法的配网GIS单线图自动生成方法研究

由于电网结构的不断复杂化,传统由发至用的结构已经发生了根本性的改变,各种信息流和能量流的交互使得配网GIS单线图的自动生成变得日益困难。针对传统人工绘图方式存在的工作量大、效率低以及准确度不受控等弊端,同时由于人为绘图习惯的影响,绘制成品的可读性较低,无法满足日益更新的电网对自身维护和管理的要求。文中结合ST-number算法,进行原图的平面性判定,实现正交成图,将复杂的电气接线信息扁平化处理。基于IEEE118节点作为算例进行实验验证,结果显示该算法可实现复杂接线图的解耦,显著提升了成图效率和绘图的准确性。

基于博弈论与改进CRITIC-AHP的配电网线路脆弱性评估

针对现有配电网线路脆弱性评估指标单一的问题,从配电网结构、运行状态和故障影响等三个方面出发,全面考虑网络拓扑结构、负荷类型、潮流分布、设备参数和线路故障等影响因素,提出了改进的线路介数、改进的线路度数、线路电压稳定性和线路潮流熵等脆弱性评估指标。为解决权重分配过程中出现的权重偏好问题,提出基于博弈论与改进CRITIC-AHP组合赋权法的综合评价模型。最后,通过在IEEE33节点系统上进行仿真,验证了所提方法的有效性。

考虑Pareto最优的列车运行图与维修天窗协调优化

列车运行图与维修天窗之间的冲突始终无法避免,且维修天窗开设时间的长短显著影响列车总运行时间。针对此问题,综合考虑维修天窗对列车运行造成的限速约束、车站到发线数量约束等,建立列车总运行时间最小,以及维修天窗实际开设时长与理想时长总偏差最小的双目标混合整数规划模型;对困难约束设置中间辅助变量将模型线性化以提高求解效率,并设计约束转换算法求解双目标模型的Pareto最优;微观化处理铁路线,将站内资源和站间资源细化为一系列行车资源单元,得到更加符合实际旅客运输需求的运行图。以某地区铁路线夜间开行列车及维修天窗开设计划为研究背景,调用商业软件求解双目标函数模型的Pareto最优,并对双目标模型的最小支配解和最优支配解进行对比分析;针对最优支配解下的列车进入、离开行车资源单元的时间、停站作业时间及维修天窗的开始时间及开设时长,绘制列车运行图。求解结果表明:模型在满足维修天窗最小开设时长的同时,能够兼顾列车运行总时间最小和维修天窗开设时长更充裕。基于最优支配解绘制的列车运行图表明:微观路网下的列车运行时刻表优化结果更符合实际旅客运输生产作业需要。研究结果可为铁路运营管理部门进一步优化列车运行图编制与维修天窗开设提供参考。

幼儿数量敏锐度的个体差异及其对数轴估计表现与策略使用的影响

以“自动关注数量信息(SFON)”倾向为指标考查4、5岁幼儿的数量敏锐度,探讨不同数量敏锐度水平的幼儿在三种形式的数轴估计(非符号面积、数字-位置(NP)和位置-数字(PN))任务上的表现及策略使用情况。结果发现:(1)4岁幼儿敏锐度可分为次高、中、低三个水平,5岁幼儿敏锐度可分为高、中两个水平,幼儿捕捉数量信息的敏锐度水平从4岁到5岁明显提升,由依赖参考提示逐渐转变为独立自发感知;(2)幼儿数轴估计表现受情境的影响,在非符号数轴估计任务上的表现显著优于在符号NP和PN任务上的表现;(3)数量敏锐度在一定程度上影响数轴估计表现及策略使用,高数量敏锐度幼儿的数轴估计成绩更好,大多数5岁幼儿基本可以在不同形式的数轴估计任务上灵活地选用合适的数轴估计策略。本研究结果揭示了幼儿心理数量表征的内部机制,证实了数量认知的四步发展模型,为鉴别、预测和筛选出低敏锐度的数学风险幼儿提供参考依据。

工位数固定的U型拆卸线部分拆卸平衡问题

为提高工位数固定的U型拆卸线拆卸效率,减少有害部件对操作人员的潜在威胁,针对高价值零部件和有害零部件的拆卸需求,本文提出了工位数固定的U型拆卸线部分拆卸平衡问题,建立了以最小化节拍时间、高危工位数目和负载均衡为目标的优化模型,并设计了改进的变邻域搜索算法进行求解.在编码过程中提出一种基于零部件释放位置的选择策略,以减少前继零部件拆卸顺序对编码的影响;提出最小偏差二分法,有效减少解码的迭代次数;提出瓶颈挤压局部搜索策略,用以优化节拍时间和均衡负载指标.通过与其他算法对比,结果表明改进的变邻域搜索算法求解具有优越性,并且可实现对工位数固定的U型拆卸线部分拆卸平衡问题的高效求解.

基于不同遗传群体的大豆分枝数QTL定位分析

分枝数是大豆重要的农艺性状,与大豆株型结构、产量潜力和适应性密切相关。为挖掘大豆分枝数稳定遗传位点,本研究以多分枝大豆Charleston和主茎型大豆东农594为亲本构建的148份重组自交系群体(RILs)和以主茎型大豆绥农14和多分枝野生大豆ZYD00006为亲本构建的213份染色体片段代换系(CSSLs)2个遗传群体为试验材料,采用ICIMapping软件中的完备区间作图法(inclusive composite interval mapping,ICIM)和Win-QTL-Cart 2.5软件中的复合区间作图法(composite interval mapping,CIM),对2018-2021年两个遗传群体的分枝数表型数据进行QTL分析,对QTL置信区间内的候选基因进行挖掘。共检测到17个与大豆分枝数性状相关的QTL位点,其中qBNA2_1和qBNK_1在不同年份和不同群体中稳定出现,分布在第8和9号染色体。根据基因注释等信息对两个QTL区间内的候选基因进行筛选,并通过qRT-PCR预测Glyma.08G053700、Glyma.08G068200、Glyma.08G082400和Glyma.09G167100为调控分枝数的候选基因。本研究结果有助于探明大豆分枝数形成的遗传机制,为大豆理想株型研究提供候选基因与材料支撑。

数字线估计的模型与策略:有界和无界的相似与分离

Cohen于2011年提出了无界数字线估计任务,诸多研究表明该任务克服了传统有界数字线估计任务的局限,能够反映更加纯粹的数字表征本质。然而,无界数字线估计的模型与策略与有界数字线估计的异同,还有待检验。文章分别对有界和无界数字线估计任务中的数字表征模型和估计策略进行梳理,分析两种任务中数字线表征模型以及估计策略发展与转变的相似与分离,并进一步推测估计策略与表征模型之间的关系,以期为数字认知的相关研究提供参考。

SOUND RADIATION FROM PLATES ON ELASTIC FOUNDATION UNDER ACTION OF MOVING LINE FORCES

An analysis is made of the problem of sound radiation from infinite one-dimensional plateson elastic foundation, when the plates are subjected to the action of harmonic line forces movingat subsonic speeds (M 【 1). The expressions of nondimensional sound power are formulated andthe asymptotic forms of sound power in the low frequency regions are derived. The radiatedsound power is shown as a function of the stiffness of elastic foundation, in terms of stiffness fac-torψ, the moving speed of line force, in terms of Math number M, and the frequency, in termsof wavenumber ratio γ . The effects of the parameter ψ in conjunction with the parameters Mand γ on the radiated sound power level and the phenomenon of coincidence radiation are alsoinvestigated in detail.

在线激光打孔参数对中支卷烟理化指标的影响

[目的]明确在线激光打孔参数对中支卷烟理化指标的影响,为中支卷烟激光打孔参数的设置提供借鉴。[方法]以物理指标、常规烟气成分释放量和感官质量为衡量指标,分析不同在线激光打孔参数对中支卷烟质量的影响。[结果]激光打孔数量与中支卷烟吸阻呈现负相关关系(P<0.01),与滤嘴通风率、总通风率呈现正相关关系(P<0.01),与TPM、水分、焦油释放量均呈现二次回归关系。激光脉冲时间与中支卷烟吸阻、滤嘴通风率、总通风率均呈现二次回归关系,但其对常规烟气成分释放量无显著影响。激光打孔数量对中支卷烟理化指标的影响程度大于激光脉冲时间,在线激光打孔参数主要影响中支卷烟的香气量、刺激性和协调性。[结论]得到一套在线激光打孔参数和中支卷烟理化指标关系的数学模型,可应用于中支卷烟开发和产品维护中激光打孔参数的设置。

考虑最少线路切换数量的电压稳定性增强优化方法

为提升电力系统的电压稳定性,提出一种基于输电线路切换的电压稳定性增强控制模型,该模型以最少的切换输电线路数量为目标来满足电力系统负荷裕度的提升需求。为灵活构建线路切换数学模型,提出基于支路追加法将线路切换融入原问题模型中。为降低模型求解难度,提出一种阶段式求解策略:基于负荷裕度对线路参数的灵敏度快速预筛选出可以提高系统负荷裕度的候选切换集,降低0-1整数变量的搜索范围;基于外逼近和松弛策略将原问题分解为2个子问题进行迭代求解,进一步提高模型的求解速度和精度。IEEE118节点和IEEE662节点系统仿真结果验证了所提模型与方法的有效性。

基于生产线换产次数对生产加工成本影响程度的方法研究

随着世界经济一体化的发展,多品种小批量等个性化需求却给众多企业带来诸多问题。本文从企业的实际需求出发,以G公司为例,构建了一个简单快捷的经济决策模型,通过对订单变化与生产成本变化的关系进行量化分析并快速决策,为企业在瞬息万变的市场竞争中赢得先机。该模型运用管理科学与工程领域相关知识对EOQ理论进行了充实和补充,可快速切入公司数字化系统实现智能决策,适用于多数劳动密集型生产企业,方法简单、高效、实用。