[ Objective] The paper aimed to study the effects of Echinacea polysaccharide on secretion of IL-8mRNA by LPS-injured IEC-6 cells, in order to figure out the mechanism of EPS on injured cells. MethodI Total RNA was e...[ Objective] The paper aimed to study the effects of Echinacea polysaccharide on secretion of IL-8mRNA by LPS-injured IEC-6 cells, in order to figure out the mechanism of EPS on injured cells. MethodI Total RNA was extracted with TRlzon reagent. IL-8 mRNA was amplified by RT-PCR and detected by agar- ose gel electrophoresis. Furthermore, electrophoresis and image was analyzed. [ Result] The 50 μg/mL EPS could partially inhibit IL-8 mRNA level produced by -stimulated IEC-6; with the increasing concentration of EPS （200 and 500 μg/mL） , the inhibitory effect against IL-8 mRNA gradually enhanced. IEC-6 was pretreated by 50, 100,200 and 500 μg/mL EPS for ：24 h, then stimulated by 10 μg/ml 1,PS for 1 and 4 h, respectively. RT-PCR analysis showed that the expres- sion of 1L-8 mRNA induced by LPS was effectively inhibited by EPS; the inhibitory effect of EPS on expression of IL-8mRNA after stimulated by LPS for 4 h was more intense and the expression concentration was lower; the inhibitory rate at 4 h was higher than that at I h. [ Conclusion] EPS protected intestinal mucosa by inhibiting secretion of IL-8 mRNA by LPS-stimulated cells, and the inhibition of EPS was dependent to concentration and time.展开更多
We report upon a case of 62 years old Saudi male with non-Hodgkin lymphoma who developed lichen planus (LP) on the inner aspect of the upper lip six months after finishing radiotherapy. The diagnosis of LP was confirm...We report upon a case of 62 years old Saudi male with non-Hodgkin lymphoma who developed lichen planus (LP) on the inner aspect of the upper lip six months after finishing radiotherapy. The diagnosis of LP was confirmed by histopathology. Literature review reveals few countable similar reports of “post radiotherapyoral LP (OLP)”. However, the isolated location of lichen planus on the upper lip per se in this case merits reporting being exceptional and reported before as isolated lichen planus of the lips (ILPL). We assume, after screening literature, that this is the first report of post radiotherapy ILPL in Arabian Gulf countries, though it may be underestimated and underreported. The etiological relation between radiotherapy and LP is discussed.展开更多
基金Supported by National Natural Science Foundation of China(31472230)Natural Science Foundation of Hebei Province(C2014407068)Project of Hebei Department of Science and Technology(14966610D)
文摘[ Objective] The paper aimed to study the effects of Echinacea polysaccharide on secretion of IL-8mRNA by LPS-injured IEC-6 cells, in order to figure out the mechanism of EPS on injured cells. MethodI Total RNA was extracted with TRlzon reagent. IL-8 mRNA was amplified by RT-PCR and detected by agar- ose gel electrophoresis. Furthermore, electrophoresis and image was analyzed. [ Result] The 50 μg/mL EPS could partially inhibit IL-8 mRNA level produced by -stimulated IEC-6; with the increasing concentration of EPS （200 and 500 μg/mL） , the inhibitory effect against IL-8 mRNA gradually enhanced. IEC-6 was pretreated by 50, 100,200 and 500 μg/mL EPS for ：24 h, then stimulated by 10 μg/ml 1,PS for 1 and 4 h, respectively. RT-PCR analysis showed that the expres- sion of 1L-8 mRNA induced by LPS was effectively inhibited by EPS; the inhibitory effect of EPS on expression of IL-8mRNA after stimulated by LPS for 4 h was more intense and the expression concentration was lower; the inhibitory rate at 4 h was higher than that at I h. [ Conclusion] EPS protected intestinal mucosa by inhibiting secretion of IL-8 mRNA by LPS-stimulated cells, and the inhibition of EPS was dependent to concentration and time.
文摘We report upon a case of 62 years old Saudi male with non-Hodgkin lymphoma who developed lichen planus (LP) on the inner aspect of the upper lip six months after finishing radiotherapy. The diagnosis of LP was confirmed by histopathology. Literature review reveals few countable similar reports of “post radiotherapyoral LP (OLP)”. However, the isolated location of lichen planus on the upper lip per se in this case merits reporting being exceptional and reported before as isolated lichen planus of the lips (ILPL). We assume, after screening literature, that this is the first report of post radiotherapy ILPL in Arabian Gulf countries, though it may be underestimated and underreported. The etiological relation between radiotherapy and LP is discussed.
