In order to study the interaction between C reactive protein (CRP) and a biomembrane, an artificial phospholipid with a long spacer, DS8PC, was synthesized. Also, a new microscope, ellipsometric microscope (EMM), wa...In order to study the interaction between C reactive protein (CRP) and a biomembrane, an artificial phospholipid with a long spacer, DS8PC, was synthesized. Also, a new microscope, ellipsometric microscope (EMM), was designed and constructed for studying film surfaces. Using the self made EMM specific interaction of rabbit C reactive protein (rCRP) with DS8PC containing monolayers was studied.展开更多
Human apolipoprotein (ApoH) is a plasma glycoprotein which belongs to the family of apolipoproteins. ApoH prefers to interact with negatively charged phospholipids in vivo, and it is believed that such interaction is ...Human apolipoprotein (ApoH) is a plasma glycoprotein which belongs to the family of apolipoproteins. ApoH prefers to interact with negatively charged phospholipids in vivo, and it is believed that such interaction is closely related to the physiological functions of ApoH and to the initialization and development of some autoimmune diseases. However, the nature of the interaction between ApoH and phospholipids is not well understood. Monolayers at an air/water interface were used to study the characteristics of the interaction between ApoH and a phospholipid membrane. The results show that ApoH is an amphipathic protein with surface activity which can insert into lipid monolayers through hydrophobic force. The critical insertion pressure of ApoH interacting with DPPS(dipalmitoyl phosphatidyl serine) and DPPC(dipalmitoyl phosphatidyl choline) monolayers is 33 5mN/m and 27 7mN/m , respectively.展开更多
A monolayer technique was used to investigate the interaction between the ribosome inactivating protein trichosanthin (TCS) and phospholipid membrane. The adsorption experiments show that the negatively charged 1,2...A monolayer technique was used to investigate the interaction between the ribosome inactivating protein trichosanthin (TCS) and phospholipid membrane. The adsorption experiments show that the negatively charged 1,2 dipalmitoyl sn glycerol 3 phosphoglycerol (DPPG) causes obvious enrichment of TCS beneath the monolayer, indicating electrostatic attraction between TCS and the negatively charged phospholipid. When TCS was incorporated into the DPPG monolayer at low pH, it could not be completely squeezed out until the monolayer collapsed. The results suggest that the electrostatic attraction and the hydrophobic force are involved in the interaction between TCS and phospholipids at different stages. These findings may be correlated with the membrane translocation mechanism of TCS.展开更多
文摘In order to study the interaction between C reactive protein (CRP) and a biomembrane, an artificial phospholipid with a long spacer, DS8PC, was synthesized. Also, a new microscope, ellipsometric microscope (EMM), was designed and constructed for studying film surfaces. Using the self made EMM specific interaction of rabbit C reactive protein (rCRP) with DS8PC containing monolayers was studied.
文摘Human apolipoprotein (ApoH) is a plasma glycoprotein which belongs to the family of apolipoproteins. ApoH prefers to interact with negatively charged phospholipids in vivo, and it is believed that such interaction is closely related to the physiological functions of ApoH and to the initialization and development of some autoimmune diseases. However, the nature of the interaction between ApoH and phospholipids is not well understood. Monolayers at an air/water interface were used to study the characteristics of the interaction between ApoH and a phospholipid membrane. The results show that ApoH is an amphipathic protein with surface activity which can insert into lipid monolayers through hydrophobic force. The critical insertion pressure of ApoH interacting with DPPS(dipalmitoyl phosphatidyl serine) and DPPC(dipalmitoyl phosphatidyl choline) monolayers is 33 5mN/m and 27 7mN/m , respectively.
基金Supported by the National Natural Science Foundation of China (No. 30 0 70 2 0 4 ) and the Graduate StudentFoundation of the Ministry of Education of China
文摘A monolayer technique was used to investigate the interaction between the ribosome inactivating protein trichosanthin (TCS) and phospholipid membrane. The adsorption experiments show that the negatively charged 1,2 dipalmitoyl sn glycerol 3 phosphoglycerol (DPPG) causes obvious enrichment of TCS beneath the monolayer, indicating electrostatic attraction between TCS and the negatively charged phospholipid. When TCS was incorporated into the DPPG monolayer at low pH, it could not be completely squeezed out until the monolayer collapsed. The results suggest that the electrostatic attraction and the hydrophobic force are involved in the interaction between TCS and phospholipids at different stages. These findings may be correlated with the membrane translocation mechanism of TCS.
