Association of glycosylated hemoglobin level with lipid ratio and individual lipids in type 2 diabetic patients

Objective:To study the correlation of lipid ratios and individual lipid indexes of patients with type 2 diabetes with glycosylated hemoglobin(HbA_(1c)).Methods:Samples were collected from 128 type 2 diabetic patients(aged 19-90 years;male 72,female 56).The sera were analyzed for HbA_(1c),total cholesterol(TC),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C) and low-density lipoprotein cholesterol(LDL-C).According to the HbA_(1e) level,the patients were divided into three groups,group A(HbA_(?)<7%,n=31),group B(7%<HbA_(1c)<10%,n=48),and group C(HbA_(1c)>10%,n=49).The correlation of HbA_(1c)with lipid ratios & individual lipid indexes were analyzed.Results:With the increased level of HbA(1c),LDL-C had a significantly increasing trend(P<0.05);whereas TC went up with the increased HbA(1c),without any significant differences between three groups.There was no significant correlation between HbA_(1c) and TG or HDL-C. With the increased level of HbA_(1c),TC/HDL-C,LDL-C/HDL-C ratios were gradually increased, with significant differences among groups(P<0.05).The lipid ratios,especially LDL-C/HDL-C ratio was more susceptible to impaired lipid metabolism in T2DM patients than individual lipid. Conclusions:LDL-C/HDL-C ratio is helpful in assessing and reducing the risk of cardiovascular disease caused by impaired lipid metabolism in type 2 diabetic patients.

Effects of Dietary Carbohydrate-lipid Ratio on Growth, Body Composition and Digestive Enzyme Activities of Juvenile Peanut Worm,Sipunculus nudus

[Objective] This study was conducted to investigate the effects of dietary carbohydrate-lipid( C / L) ratios on growth performance,body composition and digestive enzyme activities of juvenile peanut worm. [Methods]Juvenile peanut worm with average body weight of( 16. 77 ± 0. 40) mg were fed with five isonitrogenous and isoenergetic diets formulated to contain different carbohydrate / lipid( C / L) ratios( 0. 68,1. 02,1. 62,2. 61 or 4. 35) for 8 weeks. [Results] The dietary C / L ratios had no significant effects on survival of juvenile peanut worm( P 】 0. 05). With the increasing of dietary C / L ratios,weight growth rate( WGR)and specific growth rate( SGR) increased when dietary C / L ratios were between 0. 68 and 2. 61,and then decreased significantly with dietary C / L ratio further increased. When the dietary C / L ratio was 2. 61( carbohydrate level was 20. 64%,lipid level was 7. 92%) both WGR and SGR produced the maximum value,they were 451. 78 ± 16. 85% and 3. 41 ± 0. 06 % / d,respectively. The dietary C / L ratios had significant effects on body moisture,body protein content and body lipid content( P 【 0. 05),but no significant effects on body ash content of juvenile peanut worm( P 】 0. 05). Different C / L ratios had significant effects on amylase activity and lipases activity of juvenile peanut worm( P 【 0. 05),but no significant effects on protease activity( P 】 0. 05). [Conclusion]The regression model analysis showed that the most suitable dietary C / L ratio which can make juvenile peanut worm acquire the best weight growth rate is 2. 42.

Arginine Intake and Carbohydrate to Lipid Ratios Affect Gene Expression of Anabolic Hormones in Largemouth Bass, Micropterus Sahnoides

Nutritional factors influence regulation of growth hormone （GH）, insulin-like growth factor-Ⅰ （IGF-Ⅰ） and insulin （INS） in fish. But so far there are no published studies describing how single indispensable amino acids and different carbohydrate to lipid ratios influence those systems. Therefore, the present study aimed to evaluate whether arginine （Arg） intake and carbohydrate to lipid ratios would affect expression of GH, IGF-Ⅰ and INS in largemouth bass.

Causal associations between intermediate very-low-density lipoprotein cholesterol-to-total lipids ratio and peptic ulcer:A bidirectional Mendelian randomization study

BACKGROUND Previous epidemiologic investigations have consistently demonstrated a strong association between the ratio of cholesterol to total lipids in medium very-lowdensity lipoprotein(VLDL)and the occurrence of peptic ulcers(PU).However,the precise causal relationship between these factors remains ambiguous.Consequently,this study aims to elucidate the potential correlation between the ratio of cholesterol to total lipids in medium VLDL and the incidence of peptic ulcer.AIM To investigate the ratio of cholesterol to total lipids in medium very-low-density lipoprotein(VLDL)association with PU via genetic methods,guiding future clinical research.METHODS Genome-wide association study(GWAS)datasets for the ratio of cholesterol to total lipids in intermediate VLDL and peptic ulcer were retrieved from the IEU OpenGWAS project(https://gwas.mrcieu.ac.uk).For the forward Mendelian randomization(MR)analysis,72 single nucleotide polymorphisms(SNPs)were identified as instrumental variables.These SNPs were selected based on their association with the ratio of cholesterol to total lipids in intermediate VLDL,with peptic ulcer as the outcome variable.Conversely,for the inverse MR analysis,no SNPs were identified with peptic ulcer as the exposure variable and the ratio of cholesterol to total lipids in intermediate VLDL as the outcome.All MR analyses utilized inverse variance weighted(IVW)as the primary analytical method.Additionally,weighted median and MR-Egger methods were employed as supplementary analytical approaches to assess causal effects.Egger regression was used as a supplementary method to evaluate potential directional pleiotropy.Heterogeneity and multiplicity tests were conducted using the leave-one-out method to evaluate result stability and mitigate biases associated with multiple testing.RESULTS The genetically predicted ratio of cholesterol to total lipids in medium VLDL was significantly associated with an elevated risk of peptic ulcer(IVW:OR=2.557,95%CI=1.274-5.132,P=0.008).However,no causal association of peptic ulcer with the ratio of cholesterol to total lipids in medium VLDL was observed in the inverse Mendelian randomization analysis.CONCLUSION In conclusion,our study reveals a significant association between the ratio of cholesterol to total lipids in medium VLDL and an elevated risk of peptic ulcers.However,further validation through laboratory investigations and larger-scale studies is warranted to strengthen the evidence and confirm the causal relationship between these factors.

Rapid chromatographic method to decipher distinct alterations in lipid classes in NAFLD/NASH

AIM: To establish a simple method to quantify lipid classes in liver diseases and to decipher the lipid profile in p62/IMP2-2/IGF2BP2-2 transgenic mice.METHODS: Liver-specific overexpression of the insulin-like growth factor 2 mRNA binding protein p62/IMP2-2/IGF2BP2-2 was used as a model for steatosis.Steatohepatitis was induced by feeding a methioninecholine deficient diet. Steatosis was assessed histologically. For thin layer chromatographic analysis, lipids were extracted from freeze-dried tissues by hexane/2-propanol, dried, redissolved, and chromatographically separated by a two-solvent system. Dilution series of lipid standards were chromatographed, detected, andquantified. The detection was performed by either2',7'-dichlorofluoresceine or a sulfuric acid/ethanol mixture.RESULTS: Histological analyses confirmed steatosis and steatohepatitis development. The extraction,chromatographic, and detection method showed high inter-assay reproducibility and allowed quantification of the different lipid classes. The analyses confirmed an increase of triglycerides and phosphatidylethanolamine and a decrease in phosphatidylcholine in the methionine-choline deficient diet. The method was used for the first time to asses the lipid classes induced in the p62-overexpressing mouse model and showed a significant increase in all detected lipid species with a prominent increase of triglycerides by 2-fold. Interestingly, the ratio of phosphatidylcholine to phosphatidylethanolamine was decreased, as previously suggested as a marker in the progression from steatosis to steatohepatitis.CONCLUSION: The thin layer chromatography analysis allows a reliable quantification of lipid classes and provides detailed insight into the lipogenic effect of p62.

The Relationships between Erythrocyte Membrane n-6 to n-3 Polyunsaturated Fatty Acids Ratio and Blood Lipids and C-reactive Protein in Chinese Adults:An Observational Study

Objective To investigate the relationships between erythrocyte membrane n-6：n-3 PUFAs ratio and blood lipids and high sensitivity C-reactive protein （hs-CRP）. Methods The Chinese and a observational study consisted of a population-based cross-sectiona subsequent 1-year follow-up study of 171 subjects with the fasting cholesterol of 5.13-8.00 mmol/L. study of 456 plasma total Results In the cross-sectional analysis, plasma low-density lipoprotein cholesterol （LDL-c） had a significant and negative association with the erythrocyte membrane n-6：n-3 PUFAs ratio （P for trend=0.019） after adjusting for sex, age and total PUFA percentage. In the follow-up study, 171 subjects were categorized into quartiles by the changes of n-6：n-3 ratio in erythrocyte membrane （△=month 12-month 0）. In the top quartile whose ratios of n-6：n-3 increased by an average of 1.25 during the follow-up, the LDL-c-lowering extent was 3.3 times of that in the lowest quartile whose ratios of n-6：n-3 decreased by an average of 1.13 （-1.07 mmol/L v.s. -0.32 mmol/L）. The hsCRP decreased by 0.11 mg/dL in the lowest quartile while increasing by 0.10 mg/dL in the top quartile （P for difference=0.052）. Conclusion Our results suggested that the balance between n-6 and n-3 fatty acids may optimize the cardiovascular benefits from dietary PUFAs.

Effect of the administration of Psidium guava leaves on blood glucose, lipid profiles and sensitivity of the vascular mesenteric bed to Phenylephrine in streptozotocin-induced diabetic rats

The leaf of Psidium guava is traditionally used in Asia to manage, control and treat diabetes. We designed this study to elucidate the effect of the administration of oral doses of aqueous and ethanol extract from Psidium guava leaves on plasma glucose, lipid profiles and the sensitivity of the vascular mesenteric bed to Phenylephrine in diabetic and non diabetic rats. Animals were divided into 5 groups (n = 10): two groups served as non-diabetic controls (NDC), while the other groups had diabetes induced with a single injection of streptozotocin (STZ). Psidium guava-treated chronic diabetic (PSG-CD) and Psidium guava-treated controls (PSG-C) received 1g/l of Psidium guavaadded to the drinking water for 8 weeks. The mesenteric vascular beds were prepared using the McGregor method. Administration of Psidium guava caused Ca/Mg ratio, plasma glucose, high density lipoprotein (HDL), low density lipoprotein (LDL), very low density lipoprotein (VLDL), total cholesterol and triglyceride concentrations to return to normal levels, and was shown to decrease alteration in vascular reactivity to vasoconstrictor agents. Our results support the hypothesis that Psidium guava could play a role in the management of diabetes and the prevention of vascular complications in STZ-induced diabetic rats.

Effect of Different Dietary Protein and Lipid Levels on the Growth,Body Composition,and Intestinal Digestive Enzyme Activities of Juvenile Yellow Drum Nibea albiflora(Richardson)

An 8-week feeding trial was conducted to determine the optimal dietary protein-to-lipid ratio for juvenile Nibea albiflora with an initial weight of(11.76 ± 0.20) g.Nine experimental diets containing different concentrations of protein(40%,47%,or 54%) and lipids(5%,9%,or 13%) in a 3 × 3 factorial experimental design were tested in triplicate groups of fish,while the protein-to-energy(P/E) ratios of the diets varied in the range of 19.74–28.32 mg k J^(-1).Results showed that fish fed diets containing 9% or 13% lipids with 54% protein exhibited significantly higher weight gains and specific growth rates than those fed other diets.The feed conversion rate of fish fed the diet with 40% protein and 5% lipids was significantly poorer than that of fish fed other diets.The protein efficiency rate of fish fed diets with 5% lipids was significantly lower than that of fish fed 9% or 13% lipid diets.Carcass lipid and energy contents were positively correlated with dietary lipid level regardless of protein level.Fish fed a 54% protein diet showed the highest trypsin activity.The intestinal lipase activity of fish fed the diet containing 13% lipids was significantly higher than that of fish fed 5% or 9% lipid diets.These results demonstrate the high protein dietary requirements of N.albiflora.A diet containing 54% protein and 9%–13% lipids with a P/E ratio of 26.2–27.81 mg protein k J^(-1) can be considered optimal for juvenile N.albiflora.

NLR、PLR与血脂正常的老年2型糖尿病患者颈动脉粥样硬化的关系

目的 探讨血脂正常的老年2型糖尿病(T2DM)患者中性粒/淋巴细胞比值(NLR)、血小板/淋巴细胞比值(PLR)与颈动脉粥样硬化的关系。方法 选取郑州大学第一附属医院2022年1月至2023年7月收治的246例血脂水平正常的T2DM患者,对患者进行颈动脉超声检查并记录其动脉粥样硬化情况,根据有无动脉粥样硬化将患者分为单纯T2DM组(C-IMT<1 mm,即A组)120例及T2DM合并颈动脉粥样硬化组126例(C-IMT≥1 mm,即B组),检测并比较两组患者NLR、PLR、血小板、中性粒细胞、淋巴细胞等,运用多因素logistic模型探究影响T2DM患者颈动脉粥样硬化的危险因素。通过受试者工作特征(ROC)曲线评估NLR、PLR诊断T2DM患者颈动脉粥样硬化的价值。结果 与A组相比,B组NLR、PLR、血小板、中性粒细胞、白细胞水平均增高,差异有统计学意义(P<0.01)。多因素logistic模型结果显示,NLR、PLR是T2DM患者合并颈动脉粥样硬化的危险因素。ROC曲线分析显示,NLR、PLR单项或联合检测对老年T2DM患者颈动脉粥样硬化均具有较高的诊断价值(P<0.01),当NLR与PLR联合检测时曲线下面积(AUC)、特异度最高。结论 NLR、PLR是老年T2DM患者合并颈动脉粥样硬化的独立危险因素,对于是否发生颈动脉粥样硬化病变的具有诊断价值。

配合饲料糖脂比对中华绒螯蟹生长、体组成和性腺发育的影响

探究配合饲料不同糖脂比替代冰鲜鱼对中华绒螯蟹生长、体组成和性腺发育的影响。在为期6周的饲养试验中,采用5种不同糖脂比(4.09、3.00、1.93、1.40、1.05)的中华绒螯蟹配合饲料(各配合饲料分别命名为:diet 1、diet 2、diet 3、diet 4、diet 5组)替代冰鲜鱼,观察不同糖脂比下的配合饲料对中华绒螯蟹[初始平均体重(98.36±8.17)g]的生长性能、体组成、性腺指数及性腺脂肪酸的营养成分等的影响。结果显示:存活率方面,冰鲜鱼组的中华绒螯蟹成活率(63%)显著低于配合饲料组(P<0.05)。增重率方面,diet 3组(糖脂比1.93)中华绒螯蟹增重率最高[(0.89±0.07)%]。体组成和性腺发育方面,diet 3组(糖脂比1.93)中华绒螯蟹的肝胰腺中总脂肪含量[(21.11±6.14)%]显著低于其他组(P<0.05),性腺中的总脂肪含量[(38.45±6.78)%]显著高于其他组(P<0.05),性腺指数[(14.67±2.35)%]显著高于除diet 4组外的其他组(P<0.05);性腺不饱和脂肪酸含量方面,各处理组中华绒螯蟹性腺中的花生四烯酸、二十碳五烯酸和二十二碳六烯酸等脂肪酸含量存在差异,其中diet 3组(糖脂比1.93)的花生四烯酸含量显著高于其他组、二十碳五烯酸和二十二碳六烯酸含量显著高于除diet 4组以外的其他组(P<0.05)。在中华绒螯蟹初始平均体重为(98.36±8.17)g,饲养6周的试验条件下,相比冰鲜鱼组,配合饲料diet 3组(糖脂比1.93)中华绒螯蟹具有更高的存活率、增重率及性腺指数。配合饲料性腺指数的提高可能与配方中更适宜的糖脂比有关,这为营养物质从肝脏中积累进而转移到性腺中提供了物质基础和能量基础。

上海老年体检者体质量指数与血压、血脂、腰臀比及血红蛋白的关系

目的探讨老年人体质量指数(BMI)与血压(BP)、血脂、腰臀比(WHR)及血红蛋白(Hb)的关系。方法以2023年2月至2023年10月上海市宝山区某社区10978名≥65岁老年体检者为研究对象,测量身高、体质量、腰围(WC)、臀围(HC)、收缩压(SBP)、舒张压(DBP)、总胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、Hb,计算BMI、WHR。根据中国BMI参考标准将老年人分为消瘦组(<18.5 kg/m^(2),n=310)、正常体质量组(18.5 kg/m^(2)≤BMI<24 kg/m^(2),n=4692)、超重组(24 kg/m^(2)≤BMI<28 kg/m^(2),n=4615)和肥胖组(≥28 kg/m^(2),n=1361),比较4组BP、血脂、WHR、Hb水平并进行相关性分析。结果老年男性受检者BMI、WC、HC、WHR、SBP、DBP、Hb水平均高于老年女性(均P<0.01),TC、HDL、LDL水平均低于老年女性(均P<0.01)。4组的SBP、DBP、TC、HDL、LDL、WHR、Hb差异均有统计学意义(均P<0.05),且BMI与SBP、DBP、TC、LDL、WHR、Hb均正相关(r=0.109、0.064、0.041、0.042、0.108、0.089,均P<0.01),与HDL负相关(r=﹣0.106,P<0.01)。正常体质量组老年男性的BMI高于老年女性,肥胖组老年女性的BMI高于老年男性(均P<0.01)。正常体质量组年龄<75岁老年人的BMI高于年龄≥75岁老年人(P<0.05)。结论BMI与老年人健康关系密切,维持理想的BMI是老年人进行健康管理及预防慢性疾病的基础。

冠心病临界病变患者残余胆固醇/高密度脂蛋白胆固醇比值水平与冠脉CT血流储备分数的相关性

【目的】探讨冠状动脉粥样硬化性心脏病(冠心病)临界病变患者残余胆固醇/高密度脂蛋白胆固醇比值水平对冠脉CT血流储备分数(FFRct)的影响。【方法】本研究回顾性分析2022年1月1日-2022年12月31日中山大学附属第三医院心内科收治住院并行CCTA显示存在冠状动脉临界病变(管腔狭窄50%~70%)的219例患者为研究对象,其中临界病变血管304条。对以上纳入的冠脉临界病变血管行FFRct检查。FFRct以0.8为界,分为缺血组(FFRct≤0.8,N=108例)与非缺血组(FFRct>0.8,N=111例)。采用多因素Logistic回归分析探究冠心病临界病变患者FFRct≤0.8的影响因素;绘制受试者工作特征(ROC)曲线评估RC/HDL-C对FFRct≤0.8的预测价值;并通过Pear-son相关分析评估RC/HDL-C与FFRct之间是否存在相关性。【结果】缺血组中有糖尿病病史患者比例显著增高(P<0.001)。与非缺血组相比,缺血组患者RC、RC/HDL-C比值、Non-HDL-C、APOB、HbA1c、FPG水平显著增高(P<0.05)。Pearson相关性分析显示,RC/HDL-C比值、RC、Non-HDL-C、TC、TG、LDL-C、HDL-C、LP(a)、HbA1c、FPG均与FFRct值呈显著负相关(P<0.05)。单因素Logistic回归分析结果显示,糖尿病、RC、RC/HDL-C比值、Non-HDL-C、TG、LP(a)、HbA1c、FPG均与FFRct≤0.8均呈显著相关(P<0.05)。多因素Logistic回归分析表明,RC/HDL-C比值是FFRct≤0.80的预测因子(OR=4.682,95%CI 1.197~18.316,P<0.05)。【结论】冠状动脉粥样硬化性心脏病临界病变患者RC/HDL-C比值水平与FFRct≤0.8独立相关,RC/HDL-C比值可能成为评估冠状功能性缺血的潜在指标。

成人餐后血脂水平与心血管疾病风险预测关系的研究

目的探讨餐后血脂水平检测对我国成人动脉粥样硬化性心血管疾病(ASCVD)风险预测的意义。方法整群抽取2020年8月~9月于中国人民解放军总医院第六医学中心进行健康体检的35~74岁人群共979例,完善问卷调查、体格检查;分别测定其空腹及统一标准早餐后2 h的餐后血脂水平。对空腹与餐后血脂代谢特点及其与各体格测量指标:体质指数(BMI)、腰高比(WHtR)、腰围(WC)间的相关性进行分析。结果35~74岁人群自身空腹与餐后血脂水平相比,餐后三酰甘油(TG)水平较空腹时明显增高,餐后总胆固醇(TC)和高密度脂蛋白胆固醇(HDL-C)水平较空腹时均有所降低,差异均具有统计学意义(P<0.001);而空腹和餐后低密度脂蛋白胆固醇(LDL-C)水平比较无统计学差异(P>0.05)。成人空腹及餐后TG与BMI、WHtR、WC均呈正相关(P<0.01);空腹及餐后HDL-C与BMI、WHtR、WC均呈负相关(P<0.01)。餐后TG与BMI、WHtR、WC相关性均大于空腹TG。结论35~74岁人群餐后血脂与BMI、WHtR、WC相关性强,与空腹血脂一样可进行ASCVD风险的评估和预测。

MHR在慢性精神分裂症共病代谢综合征中的评估价值

目的分析单核细胞与高密度脂蛋白比值(MHR)在慢性精神分裂症代谢综合征(MS)评估中的价值。方法选取慢性精神分裂症患者100例作为研究组,另选择同期在医院体检健康体检者100例作为对照组,收集两组入选者的一般人口学资料[性别、年龄、体质量指数(BMI)]和实验室检查资料[单核细胞计数(M)、血糖(GLU)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、MHR等];依据慢性精神分裂症患者是否伴发MS分为MS组和非MS组,根据患者MHR采用四分位法分为A组(n=25)、B组(n=25)、C组(n=25)及D组(n=25);比较MS组与非MS组患者一般资料及糖脂代谢水平,分析MHR四分位法的4个亚组糖脂代谢水平及MS的发生率;采用Pearson相关性分析MHR与BMI及糖脂代谢指标的相关性、二元logistic回归分析慢性精神分裂症伴发MS的影响因素,采用受试者工作特征(ROC)曲线下面积(AUC)评估MHR在慢性精神分裂症伴发MS的诊断价值。结果与对照组比较,研究组BMI、M、TG、GLU、MHR水平升高,HDL水平降低,差异有统计学意义(P<0.05);与非MS组比较,MS组病程、年龄、BMI、GLU、TG、MHR水平增加,HDL水平降低,差异有统计学意义(P<0.05);与A组比较,B组、C组、D组BMI增加,HDL水平降低,P<0.05;与A组(8%)比较,B组(12%)、C组(28%)、D组(40%)MS发生率均升高,差异有统计学意义(P<0.05);研究组的MHR与BMI、TG呈正相关(P<0.05);与HDL水平呈负相关(P<0.05);年龄、BMI、TG、MHR均是慢性精神分裂症伴发MS的独立危险因素(P<0.05);ROC曲线分析结果显示,MHR诊断慢性精神分裂症伴发MS的AUC为0.721,95%CI为0.592~0.849,敏感度和特异度分别为77.3%、61.5%。结论MHR与慢性精神分裂症伴发MS有关,对慢性精神分裂症患者伴发MS方面具有良好的预测价值。

不同孕期血脂水平与胎盘重量和胎盘/出生体重比的相关性

目的:探讨不同孕期母体空腹血脂与胎盘重量(PW)和胎盘/出生体重比(PFR)的相关性。方法:收集规律产检及足月、顺产分娩的1172例单胎孕妇的基本信息、不同孕期空腹血脂及PW、PFR,分析母体不同孕期空腹血脂对PW、PFR的影响及其与PW和RFR的关系。结果:在排除孕前体质指数(BMI)、孕龄、产次、胎儿性别等混杂因素后,妊娠早期低密度脂蛋白C(LDL-C)每升高1个单位,PW增加9.396 g(95%CI:0.510~18.283,P=0.038);妊娠中期总胆固醇(TC)、甘油三脂(TG)每升高1个单位,PW分别增加8.582 g(95%CI:3.464~13.699,P=0.001)和7.565 g(95%CI:1.825~13.305,P=0.010);载脂蛋白a1(APOA1)每升高1个单位,PW减少31.422 g(95%CI:-49.139~-13.705,P=0.001);妊娠晚期TG每增加1个单位,PW增加5.618 g(95%CI:1.149~10.088,P=0.014);HDL-C每增加1个单位,PW减少18.622 g(95%CI:-30.047~-7.196,P=0.001)。PFR与妊娠晚期APOA1负相关(P<0.05),与妊娠晚期载脂蛋白b(APOB)正相关(P<0.05)。结论:妊娠期母体血脂异常可导致PW、PFR异常,影响胎盘功能及新生儿体重,临床需加强监测母体血脂指标变化并及时干预。

腹膜透析后感染性腹膜炎患者血清NGAL、IL-6、NLR水平观察

目的 观察腹膜透析后感染性腹膜炎患者血清中性粒细胞明胶酶相关脂质运载蛋白(NGAL)、白细胞介素-6(IL-6)水平及中性粒细胞与淋巴细胞比值(NLR)并分析其临床意义。方法 回顾性选取2020年1月至2023年12月青岛大学附属青岛市海慈医院(青岛市中医医院)收治的82例腹膜透析患者为观察组,根据患者是否发生感染性腹膜炎进一步分为感染组(n=29)、未感染组(n=53),另选择同时期、年龄段接近的在本院体检的健康者60名作为对照组。比较观察组与对照组、感染组与未感染组血清NGAL、IL-6水平及NLR。绘制受试者操作特征(ROC)曲线分析血清NGAL、IL-6水平及NLR诊断腹膜透析后感染性腹膜炎的价值。结果 观察组血清NGAL、IL-6水平及NLR分别为(462.79±85.04)μg/L、(22.74±6.18)μg/L、4.62±0.96,均明显高于对照组[(83.26±10.47)μg/L、(4.12±1.36)μg/L、2.59±0.72],差异均有统计学意义(P<0.05)。感染组血清NGAL、IL-6水平及NLR分别为(641.56±112.79)μg/L、(13.92±3.04)μg/L、7.95±1.14,均明显高于对照组[(396.37±94.06)μg/L、(29.76±8.72)μg/L、3.51±0.82],差异均有统计学意义(P<0.05)。经ROC分析,血清NGAL、IL-6水平及NLR单独及联合诊断腹膜透析后感染性腹膜炎的曲线下面积(AUC)分别为0.831(95%CI:0.767~0.895)、0.899(95%CI:0.859~0.939)、0.775(95%CI:0.696~0.854)、0.957(95%CI:0.932~0.982),敏感度分别为0.828、0.862、0.758、0.897,特异度分别为0.867、0.811、0.830、0.906。结论 腹膜透析后感染性腹膜炎患者血清NGAL、IL-6水平及NLR明显升高,通过检测以上指标对于诊断腹膜透析后感染性腹膜炎具有较好的价值,且三项指标联合检测可进一步提高诊断价值。

脂肝合剂联合多烯磷脂酰胆碱胶囊治疗非酒精性脂肪性肝病临床研究

目的:观察脂肝合剂联合多烯磷脂酰胆碱胶囊治疗痰湿内阻型非酒精性脂肪性肝病(NAFLD)的临床疗效。方法:选取76例痰湿内阻型NAFLD患者,按随机数字表法分为对照组与治疗组各38例,对照组剔除1例,治疗组剔除4例,最终纳入研究对照组37例,治疗组34例。2组均给予饮食控制加适量锻炼,对照组在此基础上给予多烯磷脂酰胆碱胶囊治疗,治疗组给予脂肝合剂联合多烯磷脂酰胆碱胶囊治疗。2组均治疗8周。比较2组临床疗效、中医证候积分、肝功能指标[谷丙转氨酶(ALT)、谷草转氨酶(AST)、谷氨酰转肽酶(GGT)]、血脂指标[甘油三酯(TG)、总胆固醇(TC)]、受控衰减参数(CAP)及肝/脾CT比值。结果:治疗后,治疗组总有效率91.18%,高于对照组72.97%(P<0.05)。2组中医证候积分均较治疗前降低(P<0.05),治疗组中医证候积分低于对照组(P<0.05)。2组血清ALT、AST、GGT、TG、TC水平均较治疗前降低(P<0.05),治疗组血清ALT、AST、GGT、TG、TC水平均低于对照组(P<0.05)。2组CAP均较治疗前降低(P<0.05),肝/脾CT比值均较治疗前升高(P<0.05);治疗组CAP低于对照组(P<0.05),肝/脾CT比值高于对照组(P<0.05)。结论:脂肝合剂联合多烯磷脂酰胆碱胶囊治疗痰湿内阻型NAFLD,可有效缓解临床症状,调节肝功能和血脂指标,减轻肝脏脂肪变性程度。

尿ACR、ɑ1-MG、NGAL对早期糖尿病肾病患者的疾病转归诊断价值分析

目的:探究尿白蛋白/肌酐比(Abumin-to-creatinine ratio,ACR)、尿ɑ1-微球蛋白(α1-microglobulin,α1-MG)、尿中性粒细胞明胶酶相关脂质运载蛋白(Neutrophil gelatinase-associated lipocalin,NGAL)对早期糖尿病肾病的诊断价值。方法:选取我院2021年10月至2022年12月期间收治的85例早期糖尿病肾病患者为研究组,并选择同期健康体检者46例为对照组,对比两组治疗前ACR、ɑ1-MG、NGAL水平,并比较不同疾病转归患者治疗前、治疗后2 m使用胶乳免疫比浊法检测上述指标水平,并使用ROC曲线分析尿ACR、ɑ1-MG、NGAL指标检测早期糖尿病肾病转归不良的诊断价值。结果:研究组尿ACR、ɑ1-MG、NGAL水平高于对照组,治疗后2 m转归不良组尿ACR、ɑ1-MGU、NGAL水平均高于转归良好组,有统计学意义(P<0.05)。经ROC曲线分析,ACR诊断早期糖尿病肾病转归不良曲线下面积(Adverse outcome curve,AUC)为0.998,ɑ1-MG诊断疾病AUC为0.916、NGAL诊断疾病AUC为0.906,灵敏度和特异度较高(P<0.05)。结论:尿ACR、ɑ1-MG、NGAL指标对早期糖尿病肾病患者转归情况具有一定的预测价值。

Folic Acid Supplementation Ameliorates Inflammation and Apoptosis in Ethanol-Induced Gastric Ulceration in Rats

Although the gastroprotective potential of folic acid has been reported, little is known about the role of inflammation and apoptosis in the said activity. This study, therefore, assessed lipid peroxidation (LPO), Neutrophil-lymphocyte ratio (NLR), C-reactive protein (CRP) as markers of inflammation and, p53 and BCl-2 as markers of apoptosis in ethanol-induced gastric ulcer pretreated with Folic acid (FA) for twenty-one (21) days. Adult male Wistar rats were arranged into experimental groups (n = 5) viz: 1) Control;2) Ulcer control;3) 2FA (2 mg/kg folic acid + Ulcer);4) 3FA (3 mg/kg folic acid + Ulcer);5) OMEP (20 mg/kg omeprazole + Ulcer);6) 2FA + OMEP + Ulcer;and 7) 3FA + OMEP + Ulcer. Ulcer score, LPO, NLR, serum CRP were all determined one hour post ulcer induction. Paraffin gastric sections were stained first with H & E, then immunostained for p53 and BCl-2. Ethanol caused gastric lesion with an index of 3.0 ± 0.2. Ulcer severity and LPO was significantly decreased in the 2FA, 3FA, OMEP, 2FA + OMEP and 3FA + OMEP groups. NLR reduced significantly in the 2FA, 3FA, OMEP and OMEP + 3FA group. Qualitatively, there was absence of C-reactive protein in the 2FA group while quantitatively, presence of CRP appeared sustained in the 3FA and OMEP treated groups. Unlike p53, the expression and labeling index of BCl-2 were significantly enhanced more in the FA and OMEP combination than OMEP alone. Folic acid ameliorates the development of gastric ulcer in rats via its anti-inflammatory and anti-apoptotic activities.

血尿酸肌酐比及血脂代谢与男性慢性阻塞性肺疾病急性加重期患者气流受限程度关系的研究

目的:通过对不同严重程度慢性阻塞性肺疾病急性加重期(acute exacerbation of chronic obstructive pulmonary disease,AECOPD)患者血尿酸(urine acid,UA)肌酐(creatinine,Cr)比(UA/Cr)及血脂代谢水平的检测,探讨男性AECOPD患者UA/Cr及血脂的表达特征及其与气流受限程度的相关性。方法:选取2017年1月—2020年10月在中山大学附属第一医院呼吸科住院的222例AECOPD男性患者作为研究对象,以及同期住院的44例男性非慢性气道疾病患者,收集相关的临床资料、肾功能、血脂及肺功能检查指标,并进行分析。结果:患者UA/Cr与FEV_(1)、BMI存在相关性,相关系数r分别为-0.325、0.162。肺功能越差,体重指数越高,UA/Cr升高越明显。而UA/Cr与年龄、吸烟史未见明显相关性。此外,高密度脂蛋白胆固醇与FEV_(1)也存在明显相关性(r=-0.231)。结论:UA/Cr及高密度脂蛋白胆固醇可在一定程度上反映男性AECOPD患者气流受限程度。