基于改进型LBP译码的LDPC码稀疏校验矩阵重建

针对LDPC码稀疏校验矩阵重建问题,基于改进型LBP译码的思想提出了一种高误码率下的LDPC码稀疏校验矩阵重建算法。首先,从码字矩阵中随机抽取部分比特构建码字分析矩阵,并对其做高斯消元求对偶空间;其次,通过判定对偶空间向量是否稀疏,提高了后续疑似校验向量判定的效率;最后,在接收码字个数不足时,利用已知校验向量结合改进型LBP译码方法纠正错误码字,加快LDPC码稀疏校验矩阵的重建速度,提高重建性能。仿真结果表明,所提算法在高误码率0.0045的条件下,对于IEEE802.11n协议下的(648,324)LDPC码,相比于现有算法,稀疏校验矩阵重建率提升了52.16%,可达到92.28%。

血清LBP、CXCL-10对小儿急性上呼吸道细菌感染的鉴别诊断价值及其影响因素

目的探讨血清脂多糖结合蛋白(LBP)、血清CXC趋化因子配体-10(CXCL-10)对小儿急性上呼吸道细菌感染的鉴别诊断价值及其影响因素。方法将2021年7月至2022年6月该院收治的90例急性上呼吸道感染患儿纳入研究作为研究组。另选取同期于本院进行体检40例健康儿童作为健康组。根据痰液细菌培养结果将研究组患儿分为细菌感染组(51例)和非细菌感染组(39例)。采用酶联免疫吸附法检测血清LBP、CXCL-10水平。采用受试者工作特征(ROC)曲线评估血清LBP、CXCL-10对小儿急性上呼吸道细菌感染的鉴别诊断价值。采用多因素Logistic回归分析小儿急性上呼吸道细菌感染的影响因素。结果研究组血清LBP、CXCL-10水平高于健康组(P<0.05)。细菌感染组血清LBP、CXCL-10水平高于非细菌感染组(P<0.05)。血清LBP、CXCL-10单独及联合用于诊断小儿急性上呼吸道细菌感染的曲线下面积(AUC)分别为0.779(95%CI:0.724~0.822)、0.843(95%CI:0.796~0.898)、0.906(95%CI:0.852~0.959)。细菌感染组家庭成员吸烟、铁元素缺乏、钙元素缺乏者所占比例、年均抗菌药使用次数、血清LBP、CXCL-10水平均高于非细菌感染患者(P<0.05)。Logistic多因素回归分析显示,年均抗菌药使用次数≥2次(OR=2.305,95%CI:1.483~3.582)、LBP≥104.26 ng/mL(OR=2.573,95%CI:1.446~4.578)、CXCL-10≥112.98 pg/mL(OR=1.208,95%CI:0.110~1.314)是小儿急性上呼吸道细菌感染的影响因素(P<0.05)。结论血清LBP、CXCL-10水平升高与儿童急性上呼吸道细菌感染密切相关,可作为鉴别诊断急性上呼吸道细菌感染的指标且二者联合诊断的效能更高。

乙肝肝硬化失代偿期并发肝功能衰竭患者血清Autotaxin、Copeptin、LBP与预后的关系研究

目的探讨乙肝肝硬化失代偿期(HBV-DC)并发肝功能衰竭(LF)患者血清自分泌运动因子(Autotaxin)、和肽素(Copeptin)、内毒素结合蛋白(LBP)与预后的关系。方法选取2018年2月至2023年8月我院收治的143例HBV-DC并发LF患者为研究对象,随访90 d,根据预后情况分组为死亡组(55例)与存活组(88例),比较两组血清Autotaxin、Copeptin、LBP水平。收集HBV-DC并发LF患者的临床资料,采用单因素和多因素Logistic回归模型分析HBV-DC并发LF患者预后的影响因素。采用受试者工作特征(ROC)曲线分析血清Autotaxin、Copeptin、LBP单独或联合预测HBV-DC并发LF患者预后的临床价值。结果143例HBV-DC并发LF患者随访90 d时,有55例死亡,88例存活,死亡率38.46%。与存活组比较,死亡组血清Autotaxin、Copeptin、LBP水平明显增加(P<0.05)。与存活组比较,死亡组住院时间≥14 d比例、并发腹水比例、并发肝性脑病比例、谷丙转氨酶、总胆红素、终末期肝病模型(MELD)评分显著升高(P<0.05),白蛋白显著降低(P<0.05),年龄、性别、合并糖尿病、合并高血压、血肌酐、血小板计数、纤维蛋白原无显著性差异(P>0.05)。总胆红素升高、并发肝性脑病、MELD评分升高以及血清Autotaxin、Copeptin、LBP水平升高均为HBV-DC并发LF患者预后不良的危险因素(P<0.05)。ROC曲线结果显示,血清Autotaxin、LBP、Copeptin标联合检测预测HBV-DC并发LF患者预后不良的曲线下面积(AUC)、灵敏度、特异度分别为0.930、85.45%、88.64%,显著优于单项指标检测预测的效能。结论血清Autotaxin、Copeptin、LBP高表达与HBV-DC并发LF患者短期死亡发生风险有关,且联合检测对HBV-DC并发LF患者短期死亡的发生具有较高的临床预测价值。

Hemorrhagic transformation in patients with large-artery atherosclerotic stroke is associated with the gut microbiota and lipopolysaccharide

Hemorrhagic transformation is a major complication of large-artery atheroscle rotic stroke(a major ischemic stro ke subtype)that wo rsens outcomes and increases mortality.Disruption of the gut microbiota is an important feature of stroke,and some specific bacteria and bacterial metabolites may contribute to hemorrhagic transformation pathogenesis.We aimed to investigate the relationship between the gut microbiota and hemorrhagic transformation in largearte ry atheroscle rotic stro ke.An observational retrospective study was conducted.From May 2020 to September 2021,blood and fecal samples were obtained upon admission from 32 patients with first-ever acute ischemic stroke and not undergoing intravenous thrombolysis or endovascular thrombectomy,as well as 16 healthy controls.Patients with stro ke who developed hemorrhagic transfo rmation(n=15)were compared to those who did not develop hemorrhagic transformation(n=17)and with healthy controls.The gut microbiota was assessed through 16S ribosomal ribonucleic acid sequencing.We also examined key components of the lipopolysaccharide pathway:lipopolysaccharide,lipopolysaccharide-binding protein,and soluble CD14.We observed that bacterial diversity was decreased in both the hemorrhagic transformation and non-hemorrhagic transfo rmation group compared with the healthy controls.The patients with ischemic stro ke who developed hemorrhagic transfo rmation exhibited altered gut micro biota composition,in particular an increase in the relative abundance and dive rsity of members belonging to the Enterobacteriaceae family.Plasma lipopolysaccharide and lipopolysaccharide-binding protein levels were higher in the hemorrhagic transformation group compared with the non-hemorrhagic transfo rmation group.lipopolysaccharide,lipopolysaccharide-binding protein,and soluble CD14 concentrations were associated with increased abundance of Enterobacte riaceae.Next,the role of the gut microbiota in hemorrhagic transformation was evaluated using an experimental stroke rat model.In this model,transplantation of the gut microbiota from hemorrhagic transformation rats into the recipient rats triggered higher plasma levels of lipopolysaccharide,lipopolysaccharide-binding protein,and soluble CD14.Ta ken togethe r,our findings demonstrate a noticeable change in the gut microbiota and lipopolysaccharide-related inflammatory response in stroke patients with hemorrhagic transformation.This suggests that maintaining a balanced gut microbiota may be an important factor in preventing hemorrhagic transfo rmation after stro ke.

基于SIFT-GMLBP的动态图像视觉信息提取研究

为了精确地提取动态图像特征,为动画设计师提供更全面、更准确的视觉信息,文中提出基于SIFT-GMLBP的动态图像视觉信息提取方法。以关键点为像素中心,采用局部二值模式(LBP),通过比较其与邻域的灰度值获取LBP码,实现动态图像局部纹理特征捕捉;根据网格化LBP(MLBP)进一步将动态图像中的像素邻域划分为多个网格,使每个网格产生一个LBP值,降低特征向量的维数;结合Gabor滤波器,通过多尺度和多方向的纹理分析,提取动态图像在不同频率和方向上的局部结构信息,整合所有Gabor滤波器响应图像的GMLBP特征,形成包含原始动态图像在不同尺度和方向上的丰富纹理信息的特征向量。实验结果表明:该方法提取的关键点数量和分布非常合理,具有较高的稳定性和动态信息捕获能力,且该方法每秒能够处理高达30帧的图像。

Quercetin Alleviates Lipopolysaccharide-Induced Cardiac Inflammation via Inhibiting Autophagy and Programmed Cell Death

Objective The aim of this study is to explore the potential modulatory role of quercetin against Endotoxin or lipopolysaccharide(LPS)induced septic cardiac dysfunction.Methods Specific pathogen-free chicken embryos(n=120)were allocated untreated control,phosphate buffer solution(PBS)vehicle,PBS with ethanol vehicle,LPS(500 ng/egg),LPS with quercetin treatment(10,20,or 40 nmol/egg,respectively),Quercetin groups(10,20,or 40 nmol/egg).Fifteenday-old embryonated eggs were inoculated with abovementioned solutions via the allantoic cavity.At embryonic day 19,the hearts of the embryos were collected for histopathological examination,RNA extraction,real-time polymerase chain reaction,immunohistochemical investigations,and Western blotting.Results They demonstrated that the heart presented inflammatory responses after LPS induction.The LPS-induced higher mRNA expressions of inflammation-related factors(TLR4,TNFα,MYD88,NF-κB1,IFNγ,IL-1β,IL-8,IL-6,IL-10,p38,MMP3,and MMP9)were blocked by quercetin with three dosages.Quercetin significantly decreased immunopositivity to TLR4 and MMP9 in the treatment group when compared with the LPS group.Quercetin significantly decreased protein expressions of TLR4,IFNγ,MMP3,and MMP9 when compared with the LPS group.Quercetin treatment prevented LPS-induced increase in the mRNA expression of Claudin 1 and ZO-1,and significantly decreased protein expression of claudin 1 when compared with the LPS group.Quercetin significantly downregulated autophagyrelated gene expressions(PPARα,SGLT1,APOA4,AMPKα1,AMPKα2,ATG5,ATG7,Beclin-1,and LC3B)and programmed cell death(Fas,Bcl-2,CASP1,CASP12,CASP3,and RIPK1)after LPS induction.Quercetin significantly decreased immunopositivity to APOA4,AMPKα2,and LC3-II/LC3-I in the treatment group when compared with the LPS group.Quercetin significantly decreased protein expressions of AMPKα1,LC3-I,and LC3-II.Quercetin significantly decreased the protein expression to CASP1 and CASP3 by immunohistochemical investigation or Western blotting in treatment group when compared with LPS group.Conclusion Quercetin alleviates cardiac inflammation induced by LPS through modulating autophagy,programmed cell death,and myocardiocytes permeability.

Natural Products Improve Organ Microcirculation Dysfunction Following Ischemia/Reperfusion-and Lipopolysaccharide-Induced Disturbances:Mechanistic and Therapeutic Views

Microcirculatory disturbances are complex processes caused by many factors,including abnormal vasomotor responses,decreased blood flow velocity,vascular endothelial cell injury,altered leukocyte and endothelial cell interactions,plasma albumin leakage,microvascular hemorrhage,and thrombosis.These disturbances involve multiple mechanisms and interactions among mechanisms that can include energy metabolism,the mitochondrial respiratory chain,oxidative stress,inflammatory factors,adhesion molecules,the cytoskeleton,vascular endothelial cells,caveolae,cell junctions,the vascular basement membrane,neutrophils,monocytes,and platelets.In clinical practice,aside from drugs that target abnormal vasomotor responses and platelet adhesion,there continues to be a lack of multi-target drugs that can regulate the complex mechanistic links and interactions underlying microcirculatory disturbances.Natural products have demonstrated obvious positive therapeutic effects in treating ischemia/reperfusion(I/R)-and lipopolysaccharide(LPS)-induced microcirculatory disturbances.In recent years,numerous research papers on the improvement of microcirculatory function by natural products have been published in international journals.In 2008 and 2017,the first listed author of this review was invited to publish reviews in the journal of Pharmacology&Therapeutics on the improvement of microcirculatory disturbances and organ injury induced by I/R using Salvia miltiorrhiza ingredients and other natural components of compounded Chinese medicine,respectively.This review systematically summarizes the effects,targets of action,and mechanisms of natural products regarding improving I/R-and LPSinduced microcirculatory disturbances and tissue injury.Based on this summary,scientific proposals are suggested for the discovery of new drugs to improve microcirculatory disturbances in disease.

Foeniculum vulgare Mill.inhibits lipopolysaccharide-induced microglia activation and ameliorates neuroinflammation-mediated behavioral deficits in mice

Objective:To investigate the effect of Foeniculum vulgare extract against lipopolysaccharide(LPS)-induced microglial activation in vitro as well as cognitive behavioral deficits in mice.Methods:LPS-activated BV-2 cell viability was measured using MTT assay and reactive oxygen species(ROS)was studied using DCF-DA assay.The antioxidative enzymes and pro-inflammatory mediators were analyzed using respective ELISA kits and Western blotting.For in vivo testing,LPS(1 mg/kg,i.p.)was given daily for five days in male Swiss albino mice to produce chronic neuroinflammation.Cognitive and behavioral tests were performed using open-field,passive avoidance,and rotarod experiments in LPS-induced mice.Results:Foeniculum vulgare extract(25,50 and 100μg/mL)significantly attenuated the LPS-activated increase in nitric oxide(NO),ROS,cyclooxygenase-2,inducible NO synthase,IL-6,and TNF-alpha(P<0.05).Moreover,LPS-induced oxidative stress and reduced antioxidative enzyme levels were significantly improved by Foeniculum vulgare extract(P<0.05).The extract also regulated the NF-κB/MAPK signaling in BV-2 cells.In an in vivo study,Foeniculum vulgare extract(50,100,and 200 mg/kg)markedly mitigated the LPS-induced cognitive and locomotor impairments in mice.The fingerprinting analysis showed distinctive peaks with rutin,kaempferol-3-O-glucoside,and anethole as identifiable compounds.Conclusions:Foeniculum vulgare extract can ameliorate LPS-stimulated neuroinflammatory responses in BV-2 microglial cells and improve cognitive and locomotor performance in LPS-administered mice.

基于LBP特征的公路气象状态快速分类方法

由于点云偏振存在特征信号离散化现象,造成分类误差较大的问题,因此提出基于LBP特征的公路气象状态快速分类方法。根据矩阵填充方法对存在缺失的偏振参量数据进行重构,获取图像多尺度信息。利用多尺度LBP获取蕴含图像纹理的空间结构,强化图像表征能力。使用点状分布的多普勒气象雷达筛选公路气象状态回波强度,获取公路气象状态雷达的极坐标扫描结果。使用贝叶斯网络分类器计算最大后验概率,划分气象状态类别取值范围,完成气象状态快速分类。由实验结果可知,该方法分类误差低于0.30,可以实现精准分类。

Antidepressant effects of Peiyuan Jieyu formula in a mouse model of chronic stress in conjunction with lipopolysaccharide-induced depression

Objective:To explore the mechanism of the Peiyuan Jieyu formula in treating depression by assessing its impact on a lipopolysaccharide-induced(LPS-induced)depression mouse model.Methods:We created a mouse model of depression by exposing mice that had previously received chronic stress to intraperitoneal LPS injections.The mice were divided into the following groups:control,model,fluoxetine,Tiansi Yin,Sini powder,and low-,medium-,and high-dose Peiyuan Jieyu formula groups.Forced swim and tail suspension tests were used to assess the efficacy of the depression(despair)model,and weight gain rates were also measured.Furthermore,serum levels of various depression and inflammation-associated molecules,including tumor necrosis factor-a(TNF-a),interferon-γ(IFN-γ),tryptophan,5-hydroxytryptamine,kynurenine(KYN),and kynurenic acid(KA)were assessed.Furthermore,the expression levels of ionic calcium-binding adaptor molecule-1(IBA-1)and indoleamine 2,3-dioxygenase(IDO)mRNA in hippocampal microglia were measured.Results:The model group displayed greater despair-associated immobility,which was shortened in response to various doses of Peiyuan Jieyu formula.Furthermore,formula administration significantly reduced serum TNF-a levels and hippocampal IDO mRNA expression.The high formula dose also reduced IFN-γand IBA-1 levels,the latter was also decreased in response to the medium formula dose.However,the low formula dose reduced serum KYN level and KYN/tryptophan(TRP)and KYN/KA ratios.Conclusion:The Peiyuan Jieyu formula holds immense potential in treating depression in a mouse model,potentially inhibiting inflammation and improving TRP-KYN metabolic disorders.

Predictive effect of lipopolysaccharide-stimulated inflammatory cytokines on symptoms of generalized anxiety disorder

BACKGROUND Generalized anxiety disorder(GAD)is a relatively common mental disorder.Recently,inflammation,an important factor for the development of depression,has attracted increasing attention.Several studies have shown that inflammatory cytokines can affect the pathophysiological processes of several nervous system diseases.We hypothesized that there is a correlation between the levels of lipopolysaccharide(LPS)-stimulated inflammatory cytokines and the clinical symptoms of GAD.AIM To investigate the predictive effect of LPS-stimulated inflammatory cytokines on symptoms of GAD.METHODS This was a cross-sectional study in which 89 patients with GAD diagnosed at The First Hospital of Hebei Medical University from January 2022 to December 2022 and 70 individuals without anxiety and depression(controls)during the same period were included.Fasting venous blood was collected from all the subjects in heparin tubes,and another 3 ml of blood was supplemented with LPS(10 ng/ml).The plasma levels of 12 cytokines[Interleukin(IL)-1β,IL-2,IL-4,IL-5,IL-6,IL-8,IL-10,tumor necrosis factor(TNF)-α,interferon(IFN)-γ,IL-17A,IL-12p70,and IFN-α]were detected.RESULTS Post-LPS stimulation,the levels of IL-1β,IL-6,IL-8,IL-10,and TNF-αin both the control and GAD groups were significantly elevated above those in the nonstimulated groups,with IL-6 and IL-8 showing marked increases.Increases in IL-8 and TNF-αwere statistically significant in the GAD group(P<0.05).IL-1β,IL-6,IL-8,IL-10,and TNF-αwere found to be significantly correlated with Hamilton Anxiety Rating Scale(HAMA)scores(P<0.05).A negative correlation was observed between IL-10 levels and HAMA scores.Further analysis revealed that TNF-αwas associated with mental anxiety,whereas IL-1β,IL-8,and IL-10 were associated with physical anxiety symptoms,with IL-10 showing a negative correlation with physical anxiety.IL-6 was associated with both mental and physical aspects of anxiety.CONCLUSION The physical symptoms of GAD are related to inflammatory factors.IL-1β,IL-8,IL-10,and TNF-a can be used as predictors of physical or mental anxiety in patients with GAD.

Adra1a调节LPS诱导的Lbp^(-/-)小鼠原代肝细胞炎症反应

目的探究Adra1a调节LPS诱导的LBP敲除小鼠(Lbp^(-/-))原代肝细胞炎症反应。方法利用二步灌流法提取WT型、Lbp^(-/-)型小鼠原代肝细胞,构建由LPS诱发的原代肝细胞原发炎症模型;采用加入抑制剂哌唑嗪、转染siRNA来下调LBP敲除小鼠原代肝细胞Adra1a的表达;抑制剂法将原代肝细胞分为3组分别是对照组A、LPS组A、抑制剂哌唑嗪组,转染siRNA主要是对原代肝细胞进行分组,包括对照组B、LPS组B、si-NC组、si-Adra1a组;将WT型小鼠的原代肝细胞分为两组分别为对照组(空白对照)、LPS组(LPS刺激12 h)。本研究以WT型、Lbp^(-/-)型小鼠原代肝细胞为研究对象利用Western blot方法验证Adra1a在LPS刺激下的变化情况,采用CCK-8、qRT-PCR、Western blot等实验方法验证哌唑嗪及si-Adra1a对Lbp^(-/-)小鼠的原代肝细胞的炎症及存活率的改善情况。结果在LPS刺激下Lbp^(-/-)小鼠的原代肝细胞Adra1a蛋白表达显著升高(P<0.01),而野生型没有显著变化;抑制剂哌唑嗪组及干扰组的细胞存活率显著升高(P<0.01,P<0.05);抑制剂哌唑嗪组及si-Adra1a组的TNF-α、IL-1β炎症因子表达情况显著降低(P<0.01),与细胞损伤及炎症相关的蛋白p-p38、p-ERK、p-JNK的表达量也显著降低(P<0.01)。结论LPS刺激Lbp^(-/-)小鼠原代肝细胞后Adra1a表达上调、炎症信号因子上调,使用哌唑嗪与si-Adra1a特异性降低Adra1a表达后使LPS相关的Lbp^(-/-)小鼠原代肝细胞炎症因子明显下降,可验证敲除LBP导致Adra1a在LPS诱导的炎症调节中参与反应。

采用LBP金字塔的人脸描述与识别

为了有效地提取人脸图像的全局和局部特征以提高人脸识别的性能,提出一种基于LBP金字塔特征的人脸描述与识别算法.首先通过多尺度分析构建人脸图像金字塔;然后采用LBP算子提取各层图像的LBP特征谱,建立图像的LBP金字塔;最后对LBP金字塔各层特征谱进行分块统计,并将各层的统计直方图序列连接起来作为人脸的鉴别特征用于分类识别.该算法在ORL和FERET人脸数据库上取得了较高的人脸识别率.实验分析表明,LBP金字塔特征具有较强的人脸描述能力和可鉴别性,且对光照、人脸表情及位置的变化具有较高的鲁棒性.

使用多尺度LBP特征描述与识别人脸

提出了一种基于多尺度LBP特征的人脸描述与识别算法。对原始人脸图像进行二级小波分解,并采用LBP算子分别计算两幅低频逼近图像的LBP特征谱;将LBP特征谱划分为若干个互不重叠的特征区域,然后分别进行直方图统计。最后,将所有区域的LBP直方图序列连接起来得到多尺度LBP特征,将其作为人脸的鉴别特征用于分类识别。所提出算法在ORL人脸数据库中取得了高达99%的人脸识别率。实验分析表明,多尺度LBP特征具有较强的人脸图像描述能力和可鉴别性,且对人脸表情及位置的变化具有较高的鲁棒性。

枸杞多糖LBP2a的分离、纯化与结构特征

经提取、分离和纯化等一系列步骤得到枸杞多糖LBP2a,用SephadexG-200柱层析和SDS-不连续体系凝胶电泳法鉴定,LBP2a为分子量均一性组分,其分子量为77482。经分光光度法测得中性糖含69.3%、半乳糖醛酸含23.8%、蛋白质含5.3%,GC-MS法测定中性糖为鼠李糖:木糖:阿拉伯糖:甘露糖:葡萄糖:半乳糖(2.62:42.85:2.13:1.00:4.36:22.80)。红外光谱分析表明LBP2a有β异头碳糖基,β-消除反应前后紫外(240nm)吸收变化及氨基酸含量变化表明LBP2a中糖链和氨基酸间有Glycan-O-Ser连接方式。

基于融合DCT和LBP特征提取的面部表情识别

针对离散余弦变换(DCT)只能提取面部表情图像的全局特征,而忽略了临近像素之间的关系、不能提取纹理特征信息、不能准确区分相似表情等问题,提出一种融合离散余弦变换方法和局部二值模式(LBP)特征的表情特征提取方法。该方法首先将人脸图像经过DCT获得的低频系数作为表情的全局特征;然后用LBP对贡献率较大的嘴部、眼睛区域进行局部纹理特征提取,通过将LBP提取到的局部纹理特征与DCT提取到的全局特征进行融合,从而得到更有效的表情特征;最后利用支持向量机(SVM)进行识别。实验结果表明:该方法比单独使用DCT方法提取的表情特征更有利于识别,提高了表情识别的准确性,并将这个表情识别方法用于智能轮椅的控制上,收到了良好的效果。

一种改进的LBP特征实现铁路扣件识别

为了提高铁路扣件基于视觉的自动化检测精度,提出了一种改进的LBP (local binary pattern)编码算法.该方法为了避免基本LBP对噪声敏感问题,根据不同邻域的不同噪声强度,结合测量误差服从高斯分布的原则,计算邻域内像素均值和偏差;根据偏差大小,自动设置阈值,实现自适应噪声抑制.为了避免基本LBP表达邻域差分关系不完整的缺陷,提出了利用邻域内随机采样的方式得到采样点对,通过比较随机点对的差分关系得到LBP编码.对在晴天、阴天、雨天等不同天气条件下的铁路扣件图像上进行实验,并与原始以及其他改进LBP进行比较.结果表明,本文的算法具有更高的检测准确率,晴天提高了3.32%,阴天提高了3.27%,雨天提高了4.10%,能够满足铁路扣件自动化检测的需要.

重组LBP真核表达载体免疫制备兔抗人LBP抗体的研究

目的 制备兔抗人脂多糖结合蛋白 (LBP)多抗 ,并研究其生物学特性。方法 用LBP基因的真核表达载体pEFBOS LBP作为抗原免疫制备兔抗人LBP的抗血清。用饱和硫酸铵盐析纯化后 ,通过ELISA检测抗体的效价 ;Westernblot鉴定其特异性 ;流式细胞术观察LBP及其抗体对荧光标记脂多糖 (LPS)与单核细胞结合的影响 ;ELISA检测LBP及其抗体对LPS诱导肺泡巨噬细胞分泌TNFα的影响。结果 用LBP基因免疫制备的兔抗LBP多抗与LBP具有良好的结合活性 ;有较高的效价 ;LBP增强LPS与单核细胞结合的作用能被该抗体阻断 ;LBP增强LPS诱导肺泡巨噬细胞分泌TNFα的作用也能被该抗体阻断。

基于LBP和深度学习的非限制条件下人脸识别算法

提出一种在非限制条件下,基于深度学习的人脸识别算法。同时,将LBP纹理特征作为深度网络的输入,通过逐层贪婪训练网络,获得良好的网络参数,并用训练好的网络对测试样本进行预测。在非限制条件下人脸库LFW上实验结果表明,该算法较传统算法(PCA、SVM、LBP)识别率高;另外,在Yale库和Yale-B库上也获得较高识别率,进一步说明以LBP纹理特征作为网络输入的深度学习方法能够对人脸图像进行准确识别。

猪LBP基因电子克隆及生物信息学分析

利用电子克隆技术,以人脂多糖结合蛋白(LBP)基因(NM_004139)为种子序列,克隆猪LBP基因。结果表明:所克隆的LBP基因开放阅读框长为1 446bp,编码481个氨基酸。推导其氨基酸序列与人、小鼠、大鼠、牛、狗的相似性分别为74.2%、64.8%、63.2%、77.1%和74.6%。进化树分析显示,猪与牛LBP基因进化距离最近,与大鼠最远。生物信息学分析表明,所克隆的猪LBP蛋白分子大小为53.036 7ku,理论等电点为6.43;亚细胞定位预测猪LBP属于分泌蛋白,主要在线粒体(44.4%)和高尔基体(22.2%)中表达;N端存在信号肽,且在25～26位氨基酸可能存在裂解位点。N端的疏水性较强,且位于信号肽处,最大疏水性值为2.478,最大亲水性值为1.956;由胞外区(1～6位氨基酸)、跨膜区(7～29位氨基酸)和胞内区(30～481位氨基酸)3个部分组成的膜蛋白,有9个Ser、8个Thr和2个Tyr,可能成为蛋白激酶磷酸化的位点;LBP膜外区蛋白由多个α螺旋,膜内区由多个β折叠构成,α螺旋和β折叠平行交替排列,构成一个弯曲螺旋状结构;在33～256和271～474位氨基酸残基之间分别有1个BPI1和BPI2的保守结构域。