The present study was conducted to investigate the effect of agitation rate on the increase in fresh weight of MD2 pineapple protocorm-like bodies (PLBs) and shoots cultured in liquid medium. PLBs were cultured in 250...The present study was conducted to investigate the effect of agitation rate on the increase in fresh weight of MD2 pineapple protocorm-like bodies (PLBs) and shoots cultured in liquid medium. PLBs were cultured in 250 ml Erlenmeyer flasks (7 g per flask) containing MS medium and plant growth regulators (1.5 mg/L 6-Benzylaminopurine, BAP and 0.2 mg/L 1-Naphthaleneacetic acid, NAA). The orbital shaker was set at speeds of 50, 80, 100, 120, and 150 rpm. After 40 days, the cultures shaken at 80 rpm showed the highest fresh weight and the highest number of shoots at 76 g and 41 shoots, respectively. A comparative study of agitation found that 80 rpm was the best speed which enhanced both PLB and shoot formation. The findings in the present study would be helpful in setting up large-scale in vitro mass propagation of MD2 pineapple.展开更多
燃油喷嘴的雾化对于解决航空发动机燃烧室问题是至关重要的,为探究某双油路离心式喷嘴的雾化性能,运用两相界面追踪流体体积(Volume of Fluid,简称VOF)方法对该喷嘴的内外部流场进行数值仿真。以双油路离心喷嘴的雾化锥角、质量流率以...燃油喷嘴的雾化对于解决航空发动机燃烧室问题是至关重要的,为探究某双油路离心式喷嘴的雾化性能,运用两相界面追踪流体体积(Volume of Fluid,简称VOF)方法对该喷嘴的内外部流场进行数值仿真。以双油路离心喷嘴的雾化锥角、质量流率以及液膜厚度作为雾化性能指标,分别模拟出主油路单独供油、副油路单独供油以及主副油路同时供油三种不同工作模式在不同压差条件下喷嘴燃油流动的稳态情况,获得双油路离心喷嘴的雾化性能指标并对其影响规律进行研究。结果显示:数值仿真能较好地模拟出喷嘴的雾化特性,随着压差增大,扩口式主油路单独工作时的雾化锥角减小,平口式副油路单独工作时的雾化锥角增大。当主、副油路同时工作时,雾化锥角随压差的增大而增大且始终处于单路单独工作时的雾化锥角之间;质量流率随着压差的增大而增大且增幅逐渐减缓;液膜厚度在低压区随压差的增大而迅速减小,随后趋于稳定。展开更多
文摘The present study was conducted to investigate the effect of agitation rate on the increase in fresh weight of MD2 pineapple protocorm-like bodies (PLBs) and shoots cultured in liquid medium. PLBs were cultured in 250 ml Erlenmeyer flasks (7 g per flask) containing MS medium and plant growth regulators (1.5 mg/L 6-Benzylaminopurine, BAP and 0.2 mg/L 1-Naphthaleneacetic acid, NAA). The orbital shaker was set at speeds of 50, 80, 100, 120, and 150 rpm. After 40 days, the cultures shaken at 80 rpm showed the highest fresh weight and the highest number of shoots at 76 g and 41 shoots, respectively. A comparative study of agitation found that 80 rpm was the best speed which enhanced both PLB and shoot formation. The findings in the present study would be helpful in setting up large-scale in vitro mass propagation of MD2 pineapple.
文摘燃油喷嘴的雾化对于解决航空发动机燃烧室问题是至关重要的,为探究某双油路离心式喷嘴的雾化性能,运用两相界面追踪流体体积(Volume of Fluid,简称VOF)方法对该喷嘴的内外部流场进行数值仿真。以双油路离心喷嘴的雾化锥角、质量流率以及液膜厚度作为雾化性能指标,分别模拟出主油路单独供油、副油路单独供油以及主副油路同时供油三种不同工作模式在不同压差条件下喷嘴燃油流动的稳态情况,获得双油路离心喷嘴的雾化性能指标并对其影响规律进行研究。结果显示:数值仿真能较好地模拟出喷嘴的雾化特性,随着压差增大,扩口式主油路单独工作时的雾化锥角减小,平口式副油路单独工作时的雾化锥角增大。当主、副油路同时工作时,雾化锥角随压差的增大而增大且始终处于单路单独工作时的雾化锥角之间;质量流率随着压差的增大而增大且增幅逐渐减缓;液膜厚度在低压区随压差的增大而迅速减小,随后趋于稳定。