Quality Evaluation and Standards Development of Liquid Spawn of Pleurotus eryngii

With four Pleurotus eryng# varieties as test materials, the mycelium pelletdensity, dry weight and diameter, fermentation broth pH value, and deoxidized sugar content were determined during their liquid fermentation to investigate the growth of mycelia and changes in fermentation broth. In addition, the sensory, biochemical and microbiological indicators of excellent P. eryngii mycelia were determined by sensory tests, microscope morphology observation and mycelium pellet back-inoculation tests. The results showed that the standards for high-quality liquid spawn of P. eryngii were as follows： bacterial liquid was light yellow and clarifiedwith rich bitter almond flavor but no bacterial contamination; mycelium pellets were visible, white and uniform with burrs on the surface, diameter 〈2.5 mm and concentration of 80%-100%; mycelium pellets were stout, and there were 3--4 clamp connectionsper visual field under the microscope; fermentation time was generally 120-144 h.

Study on Optimization of Culture Conditions for Wild Isaria cicadae Miquel Liquid Strain

[Objectives] To study the effect of different culture conditions on the growth of wild I. cicadae Miq. liquid strain. [Methods]The I. cicadae Miq. strain was inoculated into the liquid culture medium with different carbon sources,nitrogen sources,micronutrients and p H,cultured in the constant temperature shaking incubator with rotating speed of 120 r/min at 19℃,and the mycelium pellet diameter,density and weight were compared between different treatments. [Results] The results showed that the optimum components of I. cicadae Miq.strain liquid included soluble starch,milk powder and vitamin B_(12),and the optimum p H was 5. 0-6. 0. [Conclusions] Soluble starch was the most suitable carbon source for the culture medium of I. cicadae Miq. liquid strain; milk powder was the most suitable nitrogen source for the culture medium of I. cicadae Miq. liquid strain; the most suitable p H was 5. 0-9. 0 for the mycelial growth of I. cicadae Miq.; the formula and mixture ratio of the optimum culture medium for the growth of liquid strain were determined.

Liquid modified photonic crystal fiber for simultaneous temperature and strain measurement

A liquid modified photonic crystal fiber(PCF)integrated with an embedded directional coupler and multi-mode interferometer is fabricated by infiltrating three adjacent air holes of the innermost layer with standard 1.48 refractive index liquids.The refractive index of the filled liquid is higher than that of background silica,which can not only support the transmitting rod modes but also the"liquid modified core"modes propagating between the PCF core and the liquid rods.Hence,the light propagating in the liquid modified core can be efficiently coupled into the satellite waveguides under the phase-matching conditions,resulting in a dramatic decrease of the resonant wavelength intensity.Furthermore,there is a multi-mode interference produced by modified core modes and rod modes.Such a compact(~0.91 cm)device integrated with an embedded coupler and interferometer is demonstrated for high-sensitivity simultaneous temperature(~14.72 nm∕℃)and strain(~13.01 pm∕με)measurement.