Deep-Litter System is a high yield approach to raise swine with pollution free in a lower cost. In the research, based on the heat stress in summer caused by fermentation, three temperature-control systems were design...Deep-Litter System is a high yield approach to raise swine with pollution free in a lower cost. In the research, based on the heat stress in summer caused by fermentation, three temperature-control systems were designed, including natural ventilation through transoms, forced ventilation via fans, and cooling by hyperbaric spray system. Specifically, the latter intermittent auto-pressurized spray system developed in our lab, which could spray successively via pressure from storage tubes without wetting the fermentation bed, is suitable for the promotion with the deep-litter technology in rural regions , since the power consumption is only 1 kwh per day.展开更多
In this study, 43 cellulose-decomposing strains were isolated from deep-litter systems. After preliminary screening with Congo red identification medium and filter paper strip medium, five strains with large transpare...In this study, 43 cellulose-decomposing strains were isolated from deep-litter systems. After preliminary screening with Congo red identification medium and filter paper strip medium, five strains with large transparent circles that disintegrated filter paper strips were obtained. After further liquid fermentation, CMC activity, FPA activity and natural eellulase activity of these five strains were determined, and two cellulose-decomposing strains with higher enzyme activity were screened, which were named F7 and F21. Based on molecular biological identification and phylogenetic analysis of 16S rRNA gene sequences, these two cellulose- decomposing strains were identified as Bacillus subtilis and Streptomyces sp. , respectively.展开更多
Identification of management practices that can improve soil health is critical to improving the sustainability of soybean [Glycine max (L.) Merr.] production. The objective of this study was to examine the long-term ...Identification of management practices that can improve soil health is critical to improving the sustainability of soybean [Glycine max (L.) Merr.] production. The objective of this study was to examine the long-term effects of continuous soybean, corn-soybean, and soybean-cotton rotations with chicken litter and cover crops (hairy vetch, wheat, fallow) on soil health parameters, including nutrient accumulation and soil organic matter dynamics under a split plot design. The depth intervals of soil sampling were 0 - 15, 15 - 30, 30 - 60, and 60 - 90 cm. Chicken litter resulted in 62.1% and 32.8% higher water extractable organic soil N content than fallow and wheat, respectively, in the surface 0 - 15 cm of soil only. However, there was no significant difference in 1-day Solvita respiration, water extractable organic C, C/N ratio, health score, moisture, earthworm, organic matter, pH, or CEC of soil among fallow, hairy vetch, chicken litter, and wheat regardless of soil depth. Unexpectedly, annual application of chicken litter at 4.4 Mg ha−1 as an N source or growing a winter-season cover crop such as hairy vetch or wheat for continuous 16 years did not significantly increase soil organic matter or water extractable organic soil C. Annual application of chicken litter at 4.4 metric tons (Mg) ha–1 for 16 years increased soil nitrate-nitrogen (NO3−-N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), copper (Cu), and zinc (Zn) contents by 92%, 400%, 134%, 20%, 43%, 206%, and 430% in 0 - 15 cm depth compared with their initial soil values, respectively, extracted with Haney H3A-2 (2 g L–1 lithium citrate + 0.6 g L–1 citric acid + 0.4 g L–1 malic acid + 0.4 g L–1 oxalic acid) solution. The increases enhanced soil supply of these nutrients to following crops, but also increased the risks of losing them to the environment. Hairy vetch caused higher H3A extracted soil manganese (Mn) content than fallow and chicken litter in 0 - 60 cm. There was no significant difference in 1-day Solvita respiration, water extractable organic C and N, health score, moisture, organic matter, pH, CEC, or population of earthworm of soil among continuous soybean, corn-soybean, and soybean-cotton in any soil depth. Another major finding of this study was that continuous soybean exerted no adverse effect on soil health relative to the commonly used corn (Zea mays L.)-soybean and soybean-cotton (Gossypium hirsutum L.) rotations under no-tillage after 16 years. To mitigate the risks of nutrient runoff and leaching from long-term chicken litter application, we recommend reducing litter application rates and integrating cover crops into crop rotations to enhance nutrient cycling and reduce environmental impacts.展开更多
The removal of litterfall in the Eucalyptus plantations in South China affected the plant biodiversity in these ecosystems was found based on the field observation and lab analysis. The protection times of species di...The removal of litterfall in the Eucalyptus plantations in South China affected the plant biodiversity in these ecosystems was found based on the field observation and lab analysis. The protection times of species diversity of three Eucalyptus communities were different (Community Ⅰ with no protection, Community Ⅱ with 7-year-protection, Community Ⅲ with 35-year-protection). The total numbers of species in these communities (from Community Ⅰ to Ⅱ to Ⅲ) are 1, 6, and 17, respectively. The results showed that the protection of litterfall from being taken out of the ecosystem is important and can increase plant species diversity. This study combined biomass data, the chemical and physical properties of the soil, and the diversity of microbes in the communities. It is concluded that the mechanism of the effects of litter removal on biodiversity includes three factors: removing the suitable habitat of microbe and animal, decreasing the soil nutrient, and changing the special habitat for the germination and growth of invading plants. These results should have important implications for managing these Eucalyptus forest ecosystems in South China.展开更多
Decomposition dynamics were compared among green tree leaves, partially decomposed tree leaf litter (i.e., decayed tree leaf litter on forest floor) and a mixture of the two in a warm temperate forest ecosystem in c...Decomposition dynamics were compared among green tree leaves, partially decomposed tree leaf litter (i.e., decayed tree leaf litter on forest floor) and a mixture of the two in a warm temperate forest ecosystem in central China to test the influence of litter chemical quality on the degree of decomposition. The study was conducted in situ at two contrasting forest sites, an oak forest dominated by Quercus aliena var. acuteserrata Maxim., and a mixed pine and oak forest dominated by Pinus armandii Franch. and Q. aliena var. acuteserrata. We found marked differences in the rate of decomposition among litter types at both forest sites; the litter decom- position constant, k, was about 39 % greater at the oak forest site and more than 70 % greater at the pine-oak forest site, for green leaves than for partially decomposed leaf litter. The decomposition dynamics and temporal changes in litter chemistry of the three litter types also greatly differed between the two forest sites. At both forest sites, the higher rate of decomposition for the green leaves was associated with a and lower carbon to N ratio higher nitrogen (N) content (C/N) and acid-unhydrolyz- able residue to N ratio (AUR/N). We did not find any non- additive effects when mixing green leaves and partially decomposed leaf litter. Our findings support the con- tention that litter chemical quality is one of the most important determinants of litter decomposition in forest ecosystems at the local or regional scale, but the effect of litter chemical quality on decomposition differs between the contrasting forest types and may vary with the stage of decomposition.展开更多
Background:Inputs of above-and belowground litter into forest soils are changing at an unprecedented rate due to continuing human disturbances and climate change.Microorganisms drive the soil carbon(C)cycle,but the ro...Background:Inputs of above-and belowground litter into forest soils are changing at an unprecedented rate due to continuing human disturbances and climate change.Microorganisms drive the soil carbon(C)cycle,but the roles of above-and belowground litter in regulating the soil microbial community have not been evaluated at a global scale.Methods:Here,we conducted a meta-analysis based on 68 aboveground litter removal and root exclusion studies across forest ecosystems to quantify the roles of above-and belowground litter on soil microbial community and compare their relative importance.Results:Aboveground litter removal significantly declined soil microbial biomass by 4.9%but root exclusion inhibited it stronger,up to 11.7%.Moreover,the aboveground litter removal significantly raised fungi by 10.1%without altering bacteria,leading to a 46.7%increase in the fungi-to-bacteria(F/B)ratio.Differently,root exclusion significantly decreased the fungi by 26.2%but increased the bacteria by 5.7%,causing a 13.3%decrease in the F/B ratio.Specifically,root exclusion significantly inhibited arbuscular mycorrhizal fungi,ectomycorrhizal fungi,and actinomycetes by 22.9%,43.8%,and 7.9%,respectively.The negative effects of aboveground litter removal on microbial biomass increased with mean annual temperature and precipitation,whereas that of root exclusion on microbial biomass did not change with climatic factors but amplified with treatment duration.More importantly,greater effects of root exclusion on microbial biomass than aboveground litter removal were consistent across diverse forest biomes(expect boreal forests)and durations.Conclusions:These data provide a global evidence that root litter inputs exert a larger control on microbial biomass than aboveground litter inputs in forest ecosystems.Our study also highlights that changes in above-and belowground litter inputs could alter soil C stability differently by shifting the microbial community structure in the opposite direction.These findings are useful for predicting microbe-mediated C processes in response to changes in forest management or climate.展开更多
The aim of this study is to determine the influence of tree litters on soil nutrients in a managed forest in southwestern Nigeria.Mono species tree clusters consisted of indigenous species:Gmelina arborea,Tectona gran...The aim of this study is to determine the influence of tree litters on soil nutrients in a managed forest in southwestern Nigeria.Mono species tree clusters consisted of indigenous species:Gmelina arborea,Tectona grandis,Leucaena leucocephala,Bambusa vulgaris,Treculia africana,Anogeissus leiocarpus were selected and analyzed for their litter and soil chemical properties at 0–5 and 15–30 cm.T.africana and T.grandis litters have the highest nutrients,while L.leucocephala and A.leiocarpus are the lowest.Soils under G.arborea,T.africana,and A.leiocarpus are more acidic due to lower calcium and magnesium contents but have higher manganese and sulphur levels.Tree litters improved soil nitrogen,phosphorous,and micro-nutrients but depleted potassium.Litter lignin and carbon appeared to hinder the release of some nutrients.Nitrogen,potassium and copper are concentrated in the topsoil while others nutrients are not differentiated with soil depth.There is a positive correlation between litter chemistry and soil chemical properties.It was concluded that tree litter chemistry differs according to species but determines soil reaction and nutrient content.The magnitude of the effect on soil properties is related to the quality of the organic litters.展开更多
We examined how afforestation patterns impact carbon(C),nitrogen(N),and phosphorus(P)stoichiometry in the plant-litter-soil system.Plant leaf,branch,stem,and root,litter,and soil samples were collected from mixedspeci...We examined how afforestation patterns impact carbon(C),nitrogen(N),and phosphorus(P)stoichiometry in the plant-litter-soil system.Plant leaf,branch,stem,and root,litter,and soil samples were collected from mixedspecies plantations of Robinia pseudoacacia with Amygdalus davidiana(RPAD),R.pseudoacacia with Armeniaca sibirica(RPAS),and monocultures of R.pseudoacacia(RP),A.davidiana(AD),and A.sibirica(AS)in the Loess Hilly Region.The results showed that in mixed-species plantations,R.pseudoacacia had lower leaf N and P concentrations than in monocultures,while both A.davidiana and A.sibirica had higher leaf N and P concentrations.Soil P limited tree growth in both afforestation models.Mixing R.pseudoacacia with A.davidiana or A.sibirica reduced N-limitation during litter decomposition.Average soil total N and P concentrations were higher in RPAS than in RPAD,and both were higher than the corresponding monocultures.The average soil C:N ratio was the smallest in RPAS,while the average soil C:P ratio was larger in RPAS than in RP.A positive correlation between N and P concentrations,and between C:N and C:P ratios,was found in litter and all plant organs of mono-and mixedstands.Alternatively,for N concentration and C:N ratio,the correlations between plant(i.e.,leaf,branch,root)and litter and between plant and soil were inverse between plantation types.RPAD has an increased litter decomposition rate to release N and P,while RPAS has a faster rate of soil N mineralization.RPAD was the best plantation(mixed)to improve biogeochemical cycling,as soil nutrient restrictions,particularly for P-limitation,on trees growth were alleviated.This study thus provides insights into suitable tree selection and management by revealing C:N:P stoichiometry in the plant-litter-soil system under different afforestation patterns.展开更多
Trees of the genus Ficus, integral components of indigenous rainfed agro-ecosystems of the southern dry agro-climatic zone of Karnataka, southern India, have traditionally been associated with the ecological service o...Trees of the genus Ficus, integral components of indigenous rainfed agro-ecosystems of the southern dry agro-climatic zone of Karnataka, southern India, have traditionally been associated with the ecological service of soil quality enhancement in addition to various direct use benefits. We assessed the soil enrichment service of Ficus benghalensis L. a common Ficus species in these agroforestry systems, by quantifying nutrient return via litter fall. Litterfall estimation and chemical analysis of litter showed that F. benghalensis trees produce 3,512 kg ha-1 of litter annually which, on decomposition, can satisfy up to 76.70 % of N, 20.24% of P and 67.76% of K requirements of dryland crops annually per hectare. This can lead to an avoided cost of compost of US $ 36.46 ha-1·a-1 in dryland farming systems. The slow rate of decay of Ficus litter, as revealed in litter decomposition studies indicates its potential as ideal mulch for dryland soils. We discuss the complementarity between Ficus litterfall and cropping patterns in Mandya, and its implications for rainfed agricultural systems.展开更多
Research has indicated that introducing Aralia elata into larch plantations forms an agroforestry system which could provide economic benefi ts for local farmers and improve degraded soils.However,the impact of litter...Research has indicated that introducing Aralia elata into larch plantations forms an agroforestry system which could provide economic benefi ts for local farmers and improve degraded soils.However,the impact of litter mixtures on soil chemical and microbial properties in this agroforestry system are unclear,which limits effi cient management of the agroforestry system.A 365-d incubation experiment examined the eff ect of litter mixtures of diff erent proportions of larch(L)and A.elata(A)on soil chemical and microbial properties.The results show that levels of mineral N,available P,microbial biomass carbon and nitrogen,cumulative C mineralization,and activities of hydrolases and oxidases increased with an increase of A.elata in the litter mixtures.Concentration of total soil carbon,nitrogen,and phosphorous did not change(except for total nitrogen).Compared with larch litter alone,levels of mineral N,available P,microbial biomass carbon and nitrogen,cumulative C mineralization,and the activities of hydrolases and oxidases increased by 7.6–433.5%.Most chemical and microbial properties were positively correlated with mixed litter proportions and the initial levels of N,P,K,Ca,Mg,Mn,Zn and Cu in the litter,while negatively correlated with the initial concentrations of C,Fe and lignin,C/N and lignin/N ratios.The results indicate that A.elata litter can improve degraded larch soil and the degree depends on the proportion of A.elata litter in the litter mixtures.展开更多
The response of soil fauna to the litter decomposition process has received considerable attention,but this effect has not been fully examined in agroforestry systems.A 1-year in situ decomposition experiment was carr...The response of soil fauna to the litter decomposition process has received considerable attention,but this effect has not been fully examined in agroforestry systems.A 1-year in situ decomposition experiment was carried out in a pure ginkgo plantation and two ginkgo agroforestry systems using a litterbag method(11 different treatments were tested in three systems).We found that the application of different organic materials(crop residues)produced positive effects on the number of soil fauna in the ginkgo planting systems;the mixture of ginkgo leaves and corn leaves was the best performing treatment.Collembola and Acarina were the predominant groups in the litter bags and were mainly responsible for the differences among the treatments.Litter mixing promoted the abundance,richness,and diversity of soil fauna,and significant differences regarding the Shannon–Wiener index of the soil fauna were observed among the 11 treatments in July.Significantly higher soil MBC(microbial biomass carbon)and MBN(microbial biomass nitrogen)were observed in agroforestry systems than in pure ginkgo plantations.These results suggest that the practice of intercrop residue application plays an important role in enhancing soil ecosystem function in ginkgo agroforestry systems and may ultimately contribute to sustainable intercrop production,soil fertility,and local economic diversity.展开更多
Through the long-term plot study on the litter and its decomposition in the evergreen broad-leaved forest ecosystem in Hangzhou for more than two years,it was resulted that the annual litter production was 5.85 t ha^-...Through the long-term plot study on the litter and its decomposition in the evergreen broad-leaved forest ecosystem in Hangzhou for more than two years,it was resulted that the annual litter production was 5.85 t ha^-1,most of which was the fallen leave (79.5 percent) and the withered branches and fruits were far less (7.1 and 13.4 percents respectively).The dynamics of the fallen litter was shown as a curve of two-peak pattern which appeared in April and September each year.The half-life of the litter was 1.59 years.The decay rate of the litter attenuted as an exponential function.The annual amount of the nutrient returned to the ground through the litter was as large as 223.69kg ha^-1.The total current amount of the litter on the ground was 7.47t ha^-1.The decay rate in the first half of a year was 45.18 percent.This ecosystem remained in the stage of litter increasing with time.展开更多
As a linkage between plants and soil,litter decomposition and its effect on nutrient recirculation have an important ecolog‐ical significance as they contribute to soil structure improvement and the restoration of de...As a linkage between plants and soil,litter decomposition and its effect on nutrient recirculation have an important ecolog‐ical significance as they contribute to soil structure improvement and the restoration of degraded ecosystems.Fragile eco‐systems in arid regions(both hot and cold)are depleted in soil organic matter,and as a result of various factors their circu‐lation of material and energy is slower.Here we discuss how litter decomposition is necessary to maintain the stability of fragile ecosystems.We reviewed research on litter decomposition carried out in arid regions.Our objective in this review is to outline how litter decomposition,and the subsequent buildup of organic matter in soil,is a key process determining the stability of fragile ecosystems.Our review shows that existing studies have focused on the influence of single ecologi‐cal factors on litter decomposition and nutrient cycling,and highlights how the exploration of interactions among factors determining litter decomposition is still lacking.This interaction is a key aspect,since in the real world,decomposition and nutrient return to soil of litter products is affected by multiple factors.We propose a network setup on a cross-regional scale using standardized methods(e.g.,the tea bag method)to understand litter decomposition and nutrient return in frag‐ile ecosystems.Such a unique network could contribute to establish predictive models suitable for litter decomposition and nutrient return in these areas,and thus could provide theoretical and practical support for regional ecological protec‐tion and high-quality development.展开更多
Litter is an important source of easily mineralizable C, N, and P for microbial metabolism in forest ecosystems;however, its decomposition is dependent upon a variety of biotic and abiotic factors, including litter ch...Litter is an important source of easily mineralizable C, N, and P for microbial metabolism in forest ecosystems;however, its decomposition is dependent upon a variety of biotic and abiotic factors, including litter chemical composition and plant specie, soil properties, and climate. We investigated C, N, and P mineralization patterns of pine litter, oak and a mixture of various species commonly found in wetland landscape position. Litter species were incubated (alone and with soils) under laboratory conditions in the dark for 120 days. Samples were leached weekly and the leachates were analyzed for pH, E4:E6 ratio, dissolved organic carbon (DOC), total N, NO3, NH4, soluble reactive P, and total P. CO2 effluxes during the 120-d incubation period were measure using NaOH traps. Carbon loss was calculated as the sum of DOC and CO2 effluxes. Results indicated that patterns of C and N release varied with litter species and soil type. Mix species treatment resulted in larger DOC and N pulses compared to pine and oak treatments. The majority of the DOC, N, and P leached was retained by the soils. When litters were added to the soils, a greater proportion of the C was lost as CO2, while litter incubated alone lost more C as DOC. This result demonstrated the importance of the soil microbial community affecting the patterns of litter mineralization. Total N concentration and C:N ratio of the litter species were significantly correlated to C loss.展开更多
In riparian forests,litter decay provides essential energy and nutrients for both terrestrial and fluvial ecosystems.Litter mixing effects(LMEs)are crucial in regulating litter decay and nutrient dynamics,yet how LMEs...In riparian forests,litter decay provides essential energy and nutrients for both terrestrial and fluvial ecosystems.Litter mixing effects(LMEs)are crucial in regulating litter decay and nutrient dynamics,yet how LMEs change over time is unclear in riparian forests.In this study,leaf litter of three common species(Alnus sibirica Fisch.ex Turcz,Betula platyphylla Sukaczev,and Betula fruticosa Pall.)were mixed in an equal mass ratio and LMEs were measured for mass and nitrogen(N)remaining in whole litter mixtures over a 3-year period in a boreal riparian forest,northeastern China.LMEs were also assessed for component litter mass and N remaining by separating litter mixtures by species.During the decay of litter mixtures,antagonistic effects on mass and N remaining were dominant after one and two years of decay,whereas only additive effects were observed after three years.LMEs correlated negatively with functional diversity after the first and two years of decay but disappeared after three years.When sorting litter mixtures by species,non-additive LMEs on mass and N remaining decreased over incubation time.Moreover,non-additive LMEs were more frequent for litter of both B.platyphylla and B.fruticosa with lower N concentration than for A.sibirica litter with higher N concentration.These results indicate that incubation time is a key determinant of litter mixing effects during decay and highlight that late-stage litter mixture decay may be predicted from single litter decay dynamics in boreal riparian forests.展开更多
The dominant plant litter plays a crucial role in carbon(C)and nutrients cycling as well as ecosystem functions maintenance on the Qinghai-Tibet Plateau(QTP).The impact of litter decomposition of dominant plants on ed...The dominant plant litter plays a crucial role in carbon(C)and nutrients cycling as well as ecosystem functions maintenance on the Qinghai-Tibet Plateau(QTP).The impact of litter decomposition of dominant plants on edaphic parameters and grassland productivity has been extensively studied,while its decomposition processes and relevant mechanisms in this area remain poorly understood.We conducted a three-year litter decomposition experiment in the Gansu Gannan Grassland Ecosystem National Observation and Research Station,an alpine meadow ecosystem on the QTP,to investigate changes in litter enzyme activities and bacterial and fungal communities,and clarify how these critical factors regulated the decomposition of dominant plant Elymus nutans(E.nutans)litter.The results showed that cellulose and hemicellulose,which accounted for 95%of the initial lignocellulose content,were the main components in E.nutans litter decomposition.The litter enzyme activities ofβ-1,4-glucosidase(BG),β-1,4-xylosidase(BX),andβ-D-cellobiosidase(CBH)decreased with decomposition while acid phosphatase,leucine aminopeptidase,and phenol oxidase increased with decomposition.We found that both litter bacterial and fungal communities changed significantly with decomposition.Furthermore,bacterial communities shifted from copiotrophic-dominated to oligotrophic-dominated in the late stage of litter decomposition.Partial least squares path model revealed that the decomposition of E.nutans litter was mainly driven by bacterial communities and their secreted enzymes.Bacteroidota and Proteobacteria were important producers of enzymes BG,BX,and CBH,and their relative abundances were tightly positively related to the content of cellulose and hemicellulose,indicating that Bacteroidota and Proteobacteria are the main bacterial taxa of the decomposition of E.nutans litter.In conclusion,this study demonstrates that bacterial communities are the main driving forces behind the decomposition of E.nutans litter,highlighting the vital roles of bacterial communities in affecting the ecosystem functions of the QTP by regulating dominant plant litter decomposition.展开更多
Background:Forest soils in tropical and subtropical areas store a significant amount of carbon.Recent framework to assess soil organic matter(SOM)dynamics under evolving global conditions suggest that dividing bulk SO...Background:Forest soils in tropical and subtropical areas store a significant amount of carbon.Recent framework to assess soil organic matter(SOM)dynamics under evolving global conditions suggest that dividing bulk SOM into particulate and mineral-associated organic matter(POM vs.MAOM)is a promising method for identifying how SOM contributes to reducing global warming.Soil macrofauna,earthworms,and millipedes have been found to play an important role in facilitating SOM processes.However,how these two co-existing macrofaunae impac the litter decomposition process and directly impact the formation of POM and MAOM remains unclear.Methods:Here,we set up a microcosm experiment,which consisted of 20 microcosms with four treatments earthworm and litter addition(E),millipedes and litter addition(M),earthworm,millipedes,and litter addition(E+M),and control(only litter addition)in five replicates.The soil and litter were sterilized prior to beginning the incubation experiment to remove any existing microbes.After incubating the samples for 42 days,the litte properties(mass,C,and N contents),soil physicochemical properties,as well as the C and N contents,and POM and MAOM^(13)C abundance in the 0–5 and 5–10 cm soil layers were measured.Finally,the relative influences o soil physicochemical and microbial properties on the distribution of C and N in the soil fractions were analyzed Results:The litter mass,C,and N associated with all four treatments significantly decreased after incubation especially under treatment E+M(litter mass:-58.8%,litter C:-57.0%,litter N:-75.1%,respectively),while earthworm biomass significantly decreased under treatment E.Earthworm or millipede addition alone showed no significant effects on the organic carbon(OC)and total nitrogen(TN)content in the POM fraction,but join addition of both significantly increased OC and TN regardless of soil depth.Importantly,all three macrofauna treatments increased the OC and TN content and decreased the^(13)C abundance in the MAOM fraction.More than65%of the total variations in the distribution of OC and TN throughout the two fractions can be explained by a combination of soil physicochemical and microbial properties.Changes in the OC distribution in the 0–5 cm soi layer are likely due to a decrease in soil pH and an increase in arbuscular mycorrhizal fungi(AMF),while those in the 5–10 cm layer are probably caused by increases in soil exchangeable Ca and Mg,in addition to fungi and gram-negative(GN)bacteria.The observed TN distribution changes in the 0–5 cm soil likely resulted from a decrease in soil pH and increases in AMF,GN,and gram-negative(GP)bacteria,while TN distribution changes in the 5–10 cm soil could be explained by increases in exchangeable Mg and GN bacteria.Conclusions:The results indicate that the coexistence of earthworms and millipedes can accelerate the litte decomposition process and store more C in the MAOM fractions.This novel finding helps to unlock the processe by which complex SOM systems serve as C sinks in tropical forests and addresses the importance of soil mac rofauna in maintaining C-neutral atmospheric conditions under global climate change.展开更多
Addressing the challenges in detecting surface floating litter in artificial lakes,including complex environments,uneven illumination,and susceptibility to noise andweather,this paper proposes an efficient and lightwe...Addressing the challenges in detecting surface floating litter in artificial lakes,including complex environments,uneven illumination,and susceptibility to noise andweather,this paper proposes an efficient and lightweight Ghost-YOLO(You Only Look Once)v8 algorithm.The algorithmintegrates advanced attention mechanisms and a smalltarget detection head to significantly enhance detection performance and efficiency.Firstly,an SE(Squeeze-and-Excitation)mechanism is incorporated into the backbone network to fortify the extraction of resilient features and precise target localization.This mechanism models feature channel dependencies,enabling adaptive adjustment of channel importance,thereby improving recognition of floating litter targets.Secondly,a 160×160 small-target detection layer is designed in the feature fusion neck to mitigate semantic information loss due to varying target scales.This design enhances the fusion of deep and shallow semantic information,improving small target feature representation and enabling better capture and identification of tiny floating litter.Thirdly,to balance performance and efficiency,the GhostConv module replaces part of the conventional convolutions in the feature fusion neck.Additionally,a novel C2fGhost(CSPDarknet53 to 2-Stage Feature Pyramid Networks Ghost)module is introduced to further reduce network parameters.Lastly,to address the challenge of occlusion,a newloss function,WIoU(Wise Intersection over Union)v3 incorporating a flexible and non-monotonic concentration approach,is adopted to improve detection rates for surface floating litter.The outcomes of the experiments demonstrate that the Ghost-YOLO v8 model proposed in this paper performs well in the dataset Marine,significantly enhances precision and recall by 3.3 and 7.6 percentage points,respectively,in contrast with the base model,mAP@0.5 and mAP 0.5:0.95 improve by 5.3 and 4.4 percentage points and reduces the computational volume by 1.88MB,the FPS value hardly decreases,and the efficient real-time identification of floating debris on the water’s surface can be achieved costeffectively.展开更多
Despite cities being recognized as being potential sources of microplastic pollution to the wider environment, most surveys of COVID-19 plastic-based litter have been undertaken through linear transects of marine beac...Despite cities being recognized as being potential sources of microplastic pollution to the wider environment, most surveys of COVID-19 plastic-based litter have been undertaken through linear transects of marine beaches. For the far fewer number of studies conducted on inland and urban locations, the site-specific focus has primarily been surveys along the length of streets. The present study is the first to specifically assess the standing stock (i.e., moment-in-time) of littered face masks for the entire surface area of urban parking lots. The density of face masks in 50 parking lots in a Canadian coastal town (0.00054 m2 ± 0.00051 m2) was found to be significantly greater than the background level of littering of town streets. Face mask density was significantly related to visitation “usage” of parking lots as gauged by the areal size of the lots and of their onsite buildings, as well as the number of vehicles present. Neither parking lot typology nor estimates of inferred export (various measures of wind exposure) and entrapment (various metrics of obstruction) of face masks had a significant influence on the extent of whole-lot littering. In consequence, modelling of the potential input of mask-derived microplastics to the marine environment from coastal communities can use the areal density of face masks found here in association with the total surface area of lots for individual municipalities as determined through GIS analysis.展开更多
Fecal coliform bacteria such as Escherichia coli (E. coli) are one of the main sources of groundwater pollution. An assessment of the transport and Persistence of E. coli in poultry litter amended Decatur silty Clay s...Fecal coliform bacteria such as Escherichia coli (E. coli) are one of the main sources of groundwater pollution. An assessment of the transport and Persistence of E. coli in poultry litter amended Decatur silty Clay soil and Hartsells Sandy soil was conducted using soil columns and simulated groundwater leaching. Enumeration of initial E. coli was determined to range from 2.851 × 10<sup>3</sup> to 3.044 × 10<sup>3</sup> CFU per gram of soil. These results have been used in a batch study to determine the persistence rate of E. coli in Decatur silty Clay soil and Hartsells Sandy soil. Results prove that E. coli survival growth rate increases for clay soil later than and at a higher rate than sandy soil. The column study has determined that E. coli was transported at a rate of 3.7 × 10<sup>6</sup><sup> </sup>CFU for Decatur silty loam and 6.3 × 10<sup>6</sup><sup> </sup>CFU for Hartsells sandy per gram of soil. Further, linear regression analysis predictions show higher porosity and soil moisture content affect transport, and Hartsells sandy soil has higher transport of E. coli due to its higher porosity and lower volumetric water content.展开更多
基金Supported by the Agricultural Science and Technology Innovation Funds of Jiangsu(cx(12)1001-04)~~
文摘Deep-Litter System is a high yield approach to raise swine with pollution free in a lower cost. In the research, based on the heat stress in summer caused by fermentation, three temperature-control systems were designed, including natural ventilation through transoms, forced ventilation via fans, and cooling by hyperbaric spray system. Specifically, the latter intermittent auto-pressurized spray system developed in our lab, which could spray successively via pressure from storage tubes without wetting the fermentation bed, is suitable for the promotion with the deep-litter technology in rural regions , since the power consumption is only 1 kwh per day.
基金Supported by Science and Technology Development Program of Guangxi Academy of Agricultural Sciences(2015JM23,GNK2012JM10)
文摘In this study, 43 cellulose-decomposing strains were isolated from deep-litter systems. After preliminary screening with Congo red identification medium and filter paper strip medium, five strains with large transparent circles that disintegrated filter paper strips were obtained. After further liquid fermentation, CMC activity, FPA activity and natural eellulase activity of these five strains were determined, and two cellulose-decomposing strains with higher enzyme activity were screened, which were named F7 and F21. Based on molecular biological identification and phylogenetic analysis of 16S rRNA gene sequences, these two cellulose- decomposing strains were identified as Bacillus subtilis and Streptomyces sp. , respectively.
文摘Identification of management practices that can improve soil health is critical to improving the sustainability of soybean [Glycine max (L.) Merr.] production. The objective of this study was to examine the long-term effects of continuous soybean, corn-soybean, and soybean-cotton rotations with chicken litter and cover crops (hairy vetch, wheat, fallow) on soil health parameters, including nutrient accumulation and soil organic matter dynamics under a split plot design. The depth intervals of soil sampling were 0 - 15, 15 - 30, 30 - 60, and 60 - 90 cm. Chicken litter resulted in 62.1% and 32.8% higher water extractable organic soil N content than fallow and wheat, respectively, in the surface 0 - 15 cm of soil only. However, there was no significant difference in 1-day Solvita respiration, water extractable organic C, C/N ratio, health score, moisture, earthworm, organic matter, pH, or CEC of soil among fallow, hairy vetch, chicken litter, and wheat regardless of soil depth. Unexpectedly, annual application of chicken litter at 4.4 Mg ha−1 as an N source or growing a winter-season cover crop such as hairy vetch or wheat for continuous 16 years did not significantly increase soil organic matter or water extractable organic soil C. Annual application of chicken litter at 4.4 metric tons (Mg) ha–1 for 16 years increased soil nitrate-nitrogen (NO3−-N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), copper (Cu), and zinc (Zn) contents by 92%, 400%, 134%, 20%, 43%, 206%, and 430% in 0 - 15 cm depth compared with their initial soil values, respectively, extracted with Haney H3A-2 (2 g L–1 lithium citrate + 0.6 g L–1 citric acid + 0.4 g L–1 malic acid + 0.4 g L–1 oxalic acid) solution. The increases enhanced soil supply of these nutrients to following crops, but also increased the risks of losing them to the environment. Hairy vetch caused higher H3A extracted soil manganese (Mn) content than fallow and chicken litter in 0 - 60 cm. There was no significant difference in 1-day Solvita respiration, water extractable organic C and N, health score, moisture, organic matter, pH, CEC, or population of earthworm of soil among continuous soybean, corn-soybean, and soybean-cotton in any soil depth. Another major finding of this study was that continuous soybean exerted no adverse effect on soil health relative to the commonly used corn (Zea mays L.)-soybean and soybean-cotton (Gossypium hirsutum L.) rotations under no-tillage after 16 years. To mitigate the risks of nutrient runoff and leaching from long-term chicken litter application, we recommend reducing litter application rates and integrating cover crops into crop rotations to enhance nutrient cycling and reduce environmental impacts.
文摘The removal of litterfall in the Eucalyptus plantations in South China affected the plant biodiversity in these ecosystems was found based on the field observation and lab analysis. The protection times of species diversity of three Eucalyptus communities were different (Community Ⅰ with no protection, Community Ⅱ with 7-year-protection, Community Ⅲ with 35-year-protection). The total numbers of species in these communities (from Community Ⅰ to Ⅱ to Ⅲ) are 1, 6, and 17, respectively. The results showed that the protection of litterfall from being taken out of the ecosystem is important and can increase plant species diversity. This study combined biomass data, the chemical and physical properties of the soil, and the diversity of microbes in the communities. It is concluded that the mechanism of the effects of litter removal on biodiversity includes three factors: removing the suitable habitat of microbe and animal, decreasing the soil nutrient, and changing the special habitat for the germination and growth of invading plants. These results should have important implications for managing these Eucalyptus forest ecosystems in South China.
基金supported by the National Basic Research Program(Grant No.2011CB403205)
文摘Decomposition dynamics were compared among green tree leaves, partially decomposed tree leaf litter (i.e., decayed tree leaf litter on forest floor) and a mixture of the two in a warm temperate forest ecosystem in central China to test the influence of litter chemical quality on the degree of decomposition. The study was conducted in situ at two contrasting forest sites, an oak forest dominated by Quercus aliena var. acuteserrata Maxim., and a mixed pine and oak forest dominated by Pinus armandii Franch. and Q. aliena var. acuteserrata. We found marked differences in the rate of decomposition among litter types at both forest sites; the litter decom- position constant, k, was about 39 % greater at the oak forest site and more than 70 % greater at the pine-oak forest site, for green leaves than for partially decomposed leaf litter. The decomposition dynamics and temporal changes in litter chemistry of the three litter types also greatly differed between the two forest sites. At both forest sites, the higher rate of decomposition for the green leaves was associated with a and lower carbon to N ratio higher nitrogen (N) content (C/N) and acid-unhydrolyz- able residue to N ratio (AUR/N). We did not find any non- additive effects when mixing green leaves and partially decomposed leaf litter. Our findings support the con- tention that litter chemical quality is one of the most important determinants of litter decomposition in forest ecosystems at the local or regional scale, but the effect of litter chemical quality on decomposition differs between the contrasting forest types and may vary with the stage of decomposition.
基金supported by the National Natural Science Foundation of China(31830015,31901302).
文摘Background:Inputs of above-and belowground litter into forest soils are changing at an unprecedented rate due to continuing human disturbances and climate change.Microorganisms drive the soil carbon(C)cycle,but the roles of above-and belowground litter in regulating the soil microbial community have not been evaluated at a global scale.Methods:Here,we conducted a meta-analysis based on 68 aboveground litter removal and root exclusion studies across forest ecosystems to quantify the roles of above-and belowground litter on soil microbial community and compare their relative importance.Results:Aboveground litter removal significantly declined soil microbial biomass by 4.9%but root exclusion inhibited it stronger,up to 11.7%.Moreover,the aboveground litter removal significantly raised fungi by 10.1%without altering bacteria,leading to a 46.7%increase in the fungi-to-bacteria(F/B)ratio.Differently,root exclusion significantly decreased the fungi by 26.2%but increased the bacteria by 5.7%,causing a 13.3%decrease in the F/B ratio.Specifically,root exclusion significantly inhibited arbuscular mycorrhizal fungi,ectomycorrhizal fungi,and actinomycetes by 22.9%,43.8%,and 7.9%,respectively.The negative effects of aboveground litter removal on microbial biomass increased with mean annual temperature and precipitation,whereas that of root exclusion on microbial biomass did not change with climatic factors but amplified with treatment duration.More importantly,greater effects of root exclusion on microbial biomass than aboveground litter removal were consistent across diverse forest biomes(expect boreal forests)and durations.Conclusions:These data provide a global evidence that root litter inputs exert a larger control on microbial biomass than aboveground litter inputs in forest ecosystems.Our study also highlights that changes in above-and belowground litter inputs could alter soil C stability differently by shifting the microbial community structure in the opposite direction.These findings are useful for predicting microbe-mediated C processes in response to changes in forest management or climate.
文摘The aim of this study is to determine the influence of tree litters on soil nutrients in a managed forest in southwestern Nigeria.Mono species tree clusters consisted of indigenous species:Gmelina arborea,Tectona grandis,Leucaena leucocephala,Bambusa vulgaris,Treculia africana,Anogeissus leiocarpus were selected and analyzed for their litter and soil chemical properties at 0–5 and 15–30 cm.T.africana and T.grandis litters have the highest nutrients,while L.leucocephala and A.leiocarpus are the lowest.Soils under G.arborea,T.africana,and A.leiocarpus are more acidic due to lower calcium and magnesium contents but have higher manganese and sulphur levels.Tree litters improved soil nitrogen,phosphorous,and micro-nutrients but depleted potassium.Litter lignin and carbon appeared to hinder the release of some nutrients.Nitrogen,potassium and copper are concentrated in the topsoil while others nutrients are not differentiated with soil depth.There is a positive correlation between litter chemistry and soil chemical properties.It was concluded that tree litter chemistry differs according to species but determines soil reaction and nutrient content.The magnitude of the effect on soil properties is related to the quality of the organic litters.
基金funded by the National Nature Science Foundation of China (No.41771556)funded by the Spanish Government Grants PID2020-115770RB-I00 and TED2021-132627B–I00+2 种基金funded by the Spanish MCIN,AEI/10.13039/501100011033the European Union NextGenerationEU/PRTR,the Catalan government grant SGR2021-1333the Fundaci on Ram on Areces grant CIVP20A6621
文摘We examined how afforestation patterns impact carbon(C),nitrogen(N),and phosphorus(P)stoichiometry in the plant-litter-soil system.Plant leaf,branch,stem,and root,litter,and soil samples were collected from mixedspecies plantations of Robinia pseudoacacia with Amygdalus davidiana(RPAD),R.pseudoacacia with Armeniaca sibirica(RPAS),and monocultures of R.pseudoacacia(RP),A.davidiana(AD),and A.sibirica(AS)in the Loess Hilly Region.The results showed that in mixed-species plantations,R.pseudoacacia had lower leaf N and P concentrations than in monocultures,while both A.davidiana and A.sibirica had higher leaf N and P concentrations.Soil P limited tree growth in both afforestation models.Mixing R.pseudoacacia with A.davidiana or A.sibirica reduced N-limitation during litter decomposition.Average soil total N and P concentrations were higher in RPAS than in RPAD,and both were higher than the corresponding monocultures.The average soil C:N ratio was the smallest in RPAS,while the average soil C:P ratio was larger in RPAS than in RP.A positive correlation between N and P concentrations,and between C:N and C:P ratios,was found in litter and all plant organs of mono-and mixedstands.Alternatively,for N concentration and C:N ratio,the correlations between plant(i.e.,leaf,branch,root)and litter and between plant and soil were inverse between plantation types.RPAD has an increased litter decomposition rate to release N and P,while RPAS has a faster rate of soil N mineralization.RPAD was the best plantation(mixed)to improve biogeochemical cycling,as soil nutrient restrictions,particularly for P-limitation,on trees growth were alleviated.This study thus provides insights into suitable tree selection and management by revealing C:N:P stoichiometry in the plant-litter-soil system under different afforestation patterns.
基金supported by,University Grants Commission,Government of India,New Delhi and Indian Council of Forestry Research and Education,Dehra Dun
文摘Trees of the genus Ficus, integral components of indigenous rainfed agro-ecosystems of the southern dry agro-climatic zone of Karnataka, southern India, have traditionally been associated with the ecological service of soil quality enhancement in addition to various direct use benefits. We assessed the soil enrichment service of Ficus benghalensis L. a common Ficus species in these agroforestry systems, by quantifying nutrient return via litter fall. Litterfall estimation and chemical analysis of litter showed that F. benghalensis trees produce 3,512 kg ha-1 of litter annually which, on decomposition, can satisfy up to 76.70 % of N, 20.24% of P and 67.76% of K requirements of dryland crops annually per hectare. This can lead to an avoided cost of compost of US $ 36.46 ha-1·a-1 in dryland farming systems. The slow rate of decay of Ficus litter, as revealed in litter decomposition studies indicates its potential as ideal mulch for dryland soils. We discuss the complementarity between Ficus litterfall and cropping patterns in Mandya, and its implications for rainfed agricultural systems.
基金supported fi nancially by the National Natural Science Foundation of China(U1808201).
文摘Research has indicated that introducing Aralia elata into larch plantations forms an agroforestry system which could provide economic benefi ts for local farmers and improve degraded soils.However,the impact of litter mixtures on soil chemical and microbial properties in this agroforestry system are unclear,which limits effi cient management of the agroforestry system.A 365-d incubation experiment examined the eff ect of litter mixtures of diff erent proportions of larch(L)and A.elata(A)on soil chemical and microbial properties.The results show that levels of mineral N,available P,microbial biomass carbon and nitrogen,cumulative C mineralization,and activities of hydrolases and oxidases increased with an increase of A.elata in the litter mixtures.Concentration of total soil carbon,nitrogen,and phosphorous did not change(except for total nitrogen).Compared with larch litter alone,levels of mineral N,available P,microbial biomass carbon and nitrogen,cumulative C mineralization,and the activities of hydrolases and oxidases increased by 7.6–433.5%.Most chemical and microbial properties were positively correlated with mixed litter proportions and the initial levels of N,P,K,Ca,Mg,Mn,Zn and Cu in the litter,while negatively correlated with the initial concentrations of C,Fe and lignin,C/N and lignin/N ratios.The results indicate that A.elata litter can improve degraded larch soil and the degree depends on the proportion of A.elata litter in the litter mixtures.
基金supported by the Agricultural Science and Technology Independent Innovation Funds of Jiangsu Province(CX(16)1005)the National Key Research and Development Program of China(2017YFD0600700)
文摘The response of soil fauna to the litter decomposition process has received considerable attention,but this effect has not been fully examined in agroforestry systems.A 1-year in situ decomposition experiment was carried out in a pure ginkgo plantation and two ginkgo agroforestry systems using a litterbag method(11 different treatments were tested in three systems).We found that the application of different organic materials(crop residues)produced positive effects on the number of soil fauna in the ginkgo planting systems;the mixture of ginkgo leaves and corn leaves was the best performing treatment.Collembola and Acarina were the predominant groups in the litter bags and were mainly responsible for the differences among the treatments.Litter mixing promoted the abundance,richness,and diversity of soil fauna,and significant differences regarding the Shannon–Wiener index of the soil fauna were observed among the 11 treatments in July.Significantly higher soil MBC(microbial biomass carbon)and MBN(microbial biomass nitrogen)were observed in agroforestry systems than in pure ginkgo plantations.These results suggest that the practice of intercrop residue application plays an important role in enhancing soil ecosystem function in ginkgo agroforestry systems and may ultimately contribute to sustainable intercrop production,soil fertility,and local economic diversity.
基金Project supported by the Laboratory of Material Cycling in Pedosphere, Insitute of Soil Science, Chinese Academy of Sciences.
文摘Through the long-term plot study on the litter and its decomposition in the evergreen broad-leaved forest ecosystem in Hangzhou for more than two years,it was resulted that the annual litter production was 5.85 t ha^-1,most of which was the fallen leave (79.5 percent) and the withered branches and fruits were far less (7.1 and 13.4 percents respectively).The dynamics of the fallen litter was shown as a curve of two-peak pattern which appeared in April and September each year.The half-life of the litter was 1.59 years.The decay rate of the litter attenuted as an exponential function.The annual amount of the nutrient returned to the ground through the litter was as large as 223.69kg ha^-1.The total current amount of the litter on the ground was 7.47t ha^-1.The decay rate in the first half of a year was 45.18 percent.This ecosystem remained in the stage of litter increasing with time.
基金supported by the Key Research and Development Plan of Ning Xia Province,China(Grant No.2020BBF02003)the National Natural Science Foundation of China(Grant No.41877540)+1 种基金the Visiting Scholar Research Program of China Scholarship Council(Grant No.201804910131)the Second Tibetan Plateau Scientific Expedition and Research program(2019QZKK0305).
文摘As a linkage between plants and soil,litter decomposition and its effect on nutrient recirculation have an important ecolog‐ical significance as they contribute to soil structure improvement and the restoration of degraded ecosystems.Fragile eco‐systems in arid regions(both hot and cold)are depleted in soil organic matter,and as a result of various factors their circu‐lation of material and energy is slower.Here we discuss how litter decomposition is necessary to maintain the stability of fragile ecosystems.We reviewed research on litter decomposition carried out in arid regions.Our objective in this review is to outline how litter decomposition,and the subsequent buildup of organic matter in soil,is a key process determining the stability of fragile ecosystems.Our review shows that existing studies have focused on the influence of single ecologi‐cal factors on litter decomposition and nutrient cycling,and highlights how the exploration of interactions among factors determining litter decomposition is still lacking.This interaction is a key aspect,since in the real world,decomposition and nutrient return to soil of litter products is affected by multiple factors.We propose a network setup on a cross-regional scale using standardized methods(e.g.,the tea bag method)to understand litter decomposition and nutrient return in frag‐ile ecosystems.Such a unique network could contribute to establish predictive models suitable for litter decomposition and nutrient return in these areas,and thus could provide theoretical and practical support for regional ecological protec‐tion and high-quality development.
文摘Litter is an important source of easily mineralizable C, N, and P for microbial metabolism in forest ecosystems;however, its decomposition is dependent upon a variety of biotic and abiotic factors, including litter chemical composition and plant specie, soil properties, and climate. We investigated C, N, and P mineralization patterns of pine litter, oak and a mixture of various species commonly found in wetland landscape position. Litter species were incubated (alone and with soils) under laboratory conditions in the dark for 120 days. Samples were leached weekly and the leachates were analyzed for pH, E4:E6 ratio, dissolved organic carbon (DOC), total N, NO3, NH4, soluble reactive P, and total P. CO2 effluxes during the 120-d incubation period were measure using NaOH traps. Carbon loss was calculated as the sum of DOC and CO2 effluxes. Results indicated that patterns of C and N release varied with litter species and soil type. Mix species treatment resulted in larger DOC and N pulses compared to pine and oak treatments. The majority of the DOC, N, and P leached was retained by the soils. When litters were added to the soils, a greater proportion of the C was lost as CO2, while litter incubated alone lost more C as DOC. This result demonstrated the importance of the soil microbial community affecting the patterns of litter mineralization. Total N concentration and C:N ratio of the litter species were significantly correlated to C loss.
基金National Natural Science Foundation of China(41771108 and 31570479)the Natural Science Foundation of Jiangxi,China(20212ACB215002
文摘In riparian forests,litter decay provides essential energy and nutrients for both terrestrial and fluvial ecosystems.Litter mixing effects(LMEs)are crucial in regulating litter decay and nutrient dynamics,yet how LMEs change over time is unclear in riparian forests.In this study,leaf litter of three common species(Alnus sibirica Fisch.ex Turcz,Betula platyphylla Sukaczev,and Betula fruticosa Pall.)were mixed in an equal mass ratio and LMEs were measured for mass and nitrogen(N)remaining in whole litter mixtures over a 3-year period in a boreal riparian forest,northeastern China.LMEs were also assessed for component litter mass and N remaining by separating litter mixtures by species.During the decay of litter mixtures,antagonistic effects on mass and N remaining were dominant after one and two years of decay,whereas only additive effects were observed after three years.LMEs correlated negatively with functional diversity after the first and two years of decay but disappeared after three years.When sorting litter mixtures by species,non-additive LMEs on mass and N remaining decreased over incubation time.Moreover,non-additive LMEs were more frequent for litter of both B.platyphylla and B.fruticosa with lower N concentration than for A.sibirica litter with higher N concentration.These results indicate that incubation time is a key determinant of litter mixing effects during decay and highlight that late-stage litter mixture decay may be predicted from single litter decay dynamics in boreal riparian forests.
基金funded by the National Natural Science Foundation of China(31870435)the European Union's Marie Sklodowska-Curie Action Postdoctoral Fellowship(101061660)the China Scholarship Council(202106180060).
文摘The dominant plant litter plays a crucial role in carbon(C)and nutrients cycling as well as ecosystem functions maintenance on the Qinghai-Tibet Plateau(QTP).The impact of litter decomposition of dominant plants on edaphic parameters and grassland productivity has been extensively studied,while its decomposition processes and relevant mechanisms in this area remain poorly understood.We conducted a three-year litter decomposition experiment in the Gansu Gannan Grassland Ecosystem National Observation and Research Station,an alpine meadow ecosystem on the QTP,to investigate changes in litter enzyme activities and bacterial and fungal communities,and clarify how these critical factors regulated the decomposition of dominant plant Elymus nutans(E.nutans)litter.The results showed that cellulose and hemicellulose,which accounted for 95%of the initial lignocellulose content,were the main components in E.nutans litter decomposition.The litter enzyme activities ofβ-1,4-glucosidase(BG),β-1,4-xylosidase(BX),andβ-D-cellobiosidase(CBH)decreased with decomposition while acid phosphatase,leucine aminopeptidase,and phenol oxidase increased with decomposition.We found that both litter bacterial and fungal communities changed significantly with decomposition.Furthermore,bacterial communities shifted from copiotrophic-dominated to oligotrophic-dominated in the late stage of litter decomposition.Partial least squares path model revealed that the decomposition of E.nutans litter was mainly driven by bacterial communities and their secreted enzymes.Bacteroidota and Proteobacteria were important producers of enzymes BG,BX,and CBH,and their relative abundances were tightly positively related to the content of cellulose and hemicellulose,indicating that Bacteroidota and Proteobacteria are the main bacterial taxa of the decomposition of E.nutans litter.In conclusion,this study demonstrates that bacterial communities are the main driving forces behind the decomposition of E.nutans litter,highlighting the vital roles of bacterial communities in affecting the ecosystem functions of the QTP by regulating dominant plant litter decomposition.
基金supported by the GuangDong Basic and Applied Basic Research Foundation(2022A1515110439)the National Natural Science Foundation of China(32101393)+1 种基金China Postdoctoral Science Foundation(2023M7339832023M743547)。
文摘Background:Forest soils in tropical and subtropical areas store a significant amount of carbon.Recent framework to assess soil organic matter(SOM)dynamics under evolving global conditions suggest that dividing bulk SOM into particulate and mineral-associated organic matter(POM vs.MAOM)is a promising method for identifying how SOM contributes to reducing global warming.Soil macrofauna,earthworms,and millipedes have been found to play an important role in facilitating SOM processes.However,how these two co-existing macrofaunae impac the litter decomposition process and directly impact the formation of POM and MAOM remains unclear.Methods:Here,we set up a microcosm experiment,which consisted of 20 microcosms with four treatments earthworm and litter addition(E),millipedes and litter addition(M),earthworm,millipedes,and litter addition(E+M),and control(only litter addition)in five replicates.The soil and litter were sterilized prior to beginning the incubation experiment to remove any existing microbes.After incubating the samples for 42 days,the litte properties(mass,C,and N contents),soil physicochemical properties,as well as the C and N contents,and POM and MAOM^(13)C abundance in the 0–5 and 5–10 cm soil layers were measured.Finally,the relative influences o soil physicochemical and microbial properties on the distribution of C and N in the soil fractions were analyzed Results:The litter mass,C,and N associated with all four treatments significantly decreased after incubation especially under treatment E+M(litter mass:-58.8%,litter C:-57.0%,litter N:-75.1%,respectively),while earthworm biomass significantly decreased under treatment E.Earthworm or millipede addition alone showed no significant effects on the organic carbon(OC)and total nitrogen(TN)content in the POM fraction,but join addition of both significantly increased OC and TN regardless of soil depth.Importantly,all three macrofauna treatments increased the OC and TN content and decreased the^(13)C abundance in the MAOM fraction.More than65%of the total variations in the distribution of OC and TN throughout the two fractions can be explained by a combination of soil physicochemical and microbial properties.Changes in the OC distribution in the 0–5 cm soi layer are likely due to a decrease in soil pH and an increase in arbuscular mycorrhizal fungi(AMF),while those in the 5–10 cm layer are probably caused by increases in soil exchangeable Ca and Mg,in addition to fungi and gram-negative(GN)bacteria.The observed TN distribution changes in the 0–5 cm soil likely resulted from a decrease in soil pH and increases in AMF,GN,and gram-negative(GP)bacteria,while TN distribution changes in the 5–10 cm soil could be explained by increases in exchangeable Mg and GN bacteria.Conclusions:The results indicate that the coexistence of earthworms and millipedes can accelerate the litte decomposition process and store more C in the MAOM fractions.This novel finding helps to unlock the processe by which complex SOM systems serve as C sinks in tropical forests and addresses the importance of soil mac rofauna in maintaining C-neutral atmospheric conditions under global climate change.
基金Supported by the fund of the Henan Province Science and Technology Research Project(No.242102210213).
文摘Addressing the challenges in detecting surface floating litter in artificial lakes,including complex environments,uneven illumination,and susceptibility to noise andweather,this paper proposes an efficient and lightweight Ghost-YOLO(You Only Look Once)v8 algorithm.The algorithmintegrates advanced attention mechanisms and a smalltarget detection head to significantly enhance detection performance and efficiency.Firstly,an SE(Squeeze-and-Excitation)mechanism is incorporated into the backbone network to fortify the extraction of resilient features and precise target localization.This mechanism models feature channel dependencies,enabling adaptive adjustment of channel importance,thereby improving recognition of floating litter targets.Secondly,a 160×160 small-target detection layer is designed in the feature fusion neck to mitigate semantic information loss due to varying target scales.This design enhances the fusion of deep and shallow semantic information,improving small target feature representation and enabling better capture and identification of tiny floating litter.Thirdly,to balance performance and efficiency,the GhostConv module replaces part of the conventional convolutions in the feature fusion neck.Additionally,a novel C2fGhost(CSPDarknet53 to 2-Stage Feature Pyramid Networks Ghost)module is introduced to further reduce network parameters.Lastly,to address the challenge of occlusion,a newloss function,WIoU(Wise Intersection over Union)v3 incorporating a flexible and non-monotonic concentration approach,is adopted to improve detection rates for surface floating litter.The outcomes of the experiments demonstrate that the Ghost-YOLO v8 model proposed in this paper performs well in the dataset Marine,significantly enhances precision and recall by 3.3 and 7.6 percentage points,respectively,in contrast with the base model,mAP@0.5 and mAP 0.5:0.95 improve by 5.3 and 4.4 percentage points and reduces the computational volume by 1.88MB,the FPS value hardly decreases,and the efficient real-time identification of floating debris on the water’s surface can be achieved costeffectively.
文摘Despite cities being recognized as being potential sources of microplastic pollution to the wider environment, most surveys of COVID-19 plastic-based litter have been undertaken through linear transects of marine beaches. For the far fewer number of studies conducted on inland and urban locations, the site-specific focus has primarily been surveys along the length of streets. The present study is the first to specifically assess the standing stock (i.e., moment-in-time) of littered face masks for the entire surface area of urban parking lots. The density of face masks in 50 parking lots in a Canadian coastal town (0.00054 m2 ± 0.00051 m2) was found to be significantly greater than the background level of littering of town streets. Face mask density was significantly related to visitation “usage” of parking lots as gauged by the areal size of the lots and of their onsite buildings, as well as the number of vehicles present. Neither parking lot typology nor estimates of inferred export (various measures of wind exposure) and entrapment (various metrics of obstruction) of face masks had a significant influence on the extent of whole-lot littering. In consequence, modelling of the potential input of mask-derived microplastics to the marine environment from coastal communities can use the areal density of face masks found here in association with the total surface area of lots for individual municipalities as determined through GIS analysis.
文摘Fecal coliform bacteria such as Escherichia coli (E. coli) are one of the main sources of groundwater pollution. An assessment of the transport and Persistence of E. coli in poultry litter amended Decatur silty Clay soil and Hartsells Sandy soil was conducted using soil columns and simulated groundwater leaching. Enumeration of initial E. coli was determined to range from 2.851 × 10<sup>3</sup> to 3.044 × 10<sup>3</sup> CFU per gram of soil. These results have been used in a batch study to determine the persistence rate of E. coli in Decatur silty Clay soil and Hartsells Sandy soil. Results prove that E. coli survival growth rate increases for clay soil later than and at a higher rate than sandy soil. The column study has determined that E. coli was transported at a rate of 3.7 × 10<sup>6</sup><sup> </sup>CFU for Decatur silty loam and 6.3 × 10<sup>6</sup><sup> </sup>CFU for Hartsells sandy per gram of soil. Further, linear regression analysis predictions show higher porosity and soil moisture content affect transport, and Hartsells sandy soil has higher transport of E. coli due to its higher porosity and lower volumetric water content.