BACKGROUND: The configuration and course of liver blood vessels (LBVs) are involved in the study of patho-genesis of hepatic diseases including liver cirrhosis, tissue engineering of the liver and surgical treatment o...BACKGROUND: The configuration and course of liver blood vessels (LBVs) are involved in the study of patho-genesis of hepatic diseases including liver cirrhosis, tissue engineering of the liver and surgical treatment of diseases of the liver and gallbladder. In the study of vascularization in tissue engineering of the liver in particular, the work we should do is to get the anatomy data of LBVs for computer-aided reconstruction of digital model of LBVs. In doing so, the casting sample of rat liver blood vessels (RLBVs) is fabricated and the data of each section of the sample is harvested. METHODS: Liquid polymer preparation (8%-10%), which was made of chlorinated poly vinyl chloride ( CPVC) as a solute, acetone as solvent and pigment, was injected into the RLBVs of 40 rats. Once acetone evaporated, the preparation solidified. When the cells and connective tissue were dissolved by hydrochloric acid, a casting sample of RLBV was left. The sample was embedded in paraffin and cut into sections. The data of each section of RLBVs was collected by digital camera. RESULTS: In 36 rats, the casting sample of RLBVs was made successfully by this method. The diameter of the hepatic arteries varied from 0. 8 to 0.2 mm, the portal veins from 2.0 to 0.1 mm, and the hepatic veins from 2.2 to 0.2 mm. In each rat, about 150 photographs of the sections of RLBVs were taken. CONCLUSION: The method described above is feasible for getting experimental data for computer-aided reconstruction of the digital model of RLBVs.展开更多
AIM:To investigate the pathogenesis of biliary casts after liver transplantation relative to their morphology and biochemical markers.METHODS:The microstructure of biliary casts was assessed using scanning electron mi...AIM:To investigate the pathogenesis of biliary casts after liver transplantation relative to their morphology and biochemical markers.METHODS:The microstructure of biliary casts was assessed using scanning electron microscopy and Hematoxylin and eosin staining assessed their histology.The expression levels of CD3,CD5,CD34,CD68 and CD79a in these biliary casts were evaluated immunohistochemically.RESULTS:Biliary casts differed widely in their microstructure,with some containing blood vessels positive for CD34 and collagen fibers with positive Masson staining.Large numbers of neutrophils and other inflammatory cells were present,but only on the edge of the biliary casts;although the boundaries were clear without crossover.None of the biliary casts contained T-lymphocytes,B-lymphocytes,macrophages and other inflammatory cells.CONCLUSION:The microcostructure of biliary casts differed.Bacteria and acute rejection are not clearly related to their formation.展开更多
文摘BACKGROUND: The configuration and course of liver blood vessels (LBVs) are involved in the study of patho-genesis of hepatic diseases including liver cirrhosis, tissue engineering of the liver and surgical treatment of diseases of the liver and gallbladder. In the study of vascularization in tissue engineering of the liver in particular, the work we should do is to get the anatomy data of LBVs for computer-aided reconstruction of digital model of LBVs. In doing so, the casting sample of rat liver blood vessels (RLBVs) is fabricated and the data of each section of the sample is harvested. METHODS: Liquid polymer preparation (8%-10%), which was made of chlorinated poly vinyl chloride ( CPVC) as a solute, acetone as solvent and pigment, was injected into the RLBVs of 40 rats. Once acetone evaporated, the preparation solidified. When the cells and connective tissue were dissolved by hydrochloric acid, a casting sample of RLBV was left. The sample was embedded in paraffin and cut into sections. The data of each section of RLBVs was collected by digital camera. RESULTS: In 36 rats, the casting sample of RLBVs was made successfully by this method. The diameter of the hepatic arteries varied from 0. 8 to 0.2 mm, the portal veins from 2.0 to 0.1 mm, and the hepatic veins from 2.2 to 0.2 mm. In each rat, about 150 photographs of the sections of RLBVs were taken. CONCLUSION: The method described above is feasible for getting experimental data for computer-aided reconstruction of the digital model of RLBVs.
文摘AIM:To investigate the pathogenesis of biliary casts after liver transplantation relative to their morphology and biochemical markers.METHODS:The microstructure of biliary casts was assessed using scanning electron microscopy and Hematoxylin and eosin staining assessed their histology.The expression levels of CD3,CD5,CD34,CD68 and CD79a in these biliary casts were evaluated immunohistochemically.RESULTS:Biliary casts differed widely in their microstructure,with some containing blood vessels positive for CD34 and collagen fibers with positive Masson staining.Large numbers of neutrophils and other inflammatory cells were present,but only on the edge of the biliary casts;although the boundaries were clear without crossover.None of the biliary casts contained T-lymphocytes,B-lymphocytes,macrophages and other inflammatory cells.CONCLUSION:The microcostructure of biliary casts differed.Bacteria and acute rejection are not clearly related to their formation.
