A dual-RPA based lateral flow strip for sensitive,on-site detection of CP4-EPSPS and Cry1Ab/Ac genes in genetically modified crops

Traditional transgenic detection methods require high test conditions and struggle to be both sensitive and efficient.In this study,a one-tube dual recombinase polymerase amplification(RPA)reaction system for CP4-EPSPS and Cry1Ab/Ac was proposed and combined with a lateral flow immunochromatographic assay,named“Dual-RPA-LFD”,to visualize the dual detection of genetically modified(GM)crops.In which,the herbicide tolerance gene CP4-EPSPS and the insect resistance gene Cry1Ab/Ac were selected as targets taking into account the current status of the most widespread application of insect resistance and herbicide tolerance traits and their stacked traits.Gradient diluted plasmids,transgenic standards,and actual samples were used as templates to conduct sensitivity,specificity,and practicality assays,respectively.The constructed method achieved the visual detection of plasmid at levels as low as 100 copies,demonstrating its high sensitivity.In addition,good applicability to transgenic samples was observed,with no cross-interference between two test lines and no influence from other genes.In conclusion,this strategy achieved the expected purpose of simultaneous detection of the two popular targets in GM crops within 20 min at 37°C in a rapid,equipmentfree field manner,providing a new alternative for rapid screening for transgenic assays in the field.

Cheesy material on macroscopic on-site evaluation after endoscopic ultrasound-guided fine-needle biopsy:Don't miss the tuberculosis

Endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)is an excellent investigation to diagnose pancreatic lesions and has shown high accuracy for its use in pathologic diagnosis.Recently,macroscopic on-site evaluation(MOSE)performed by an endoscopist was introduced as an alternative to rapid on-site cytologic evaluation to increase the diagnostic yield of EUS-FNB.The MOSE of the biopsy can estimate the adequacy of the sample directly by the macroscopic evaluation of the core tissue obtained from EUS-FNB.Isolated pancreatic tuberculosis is extremely rare and difficult to diagnose because of its non-specific signs and symptoms.Therefore,this challenging diagnosis is based on endoscopy,imaging,and the bacteriological and histological examination of tissue biopsies.This uncommon presentation of tuberculosis can be revealed as pancreatic mass mimicking cancer.EUS-FNB can be very useful in providing a valuable histopathological diagnosis.A calcified lesion with a cheesy core in MOSE must be suggestive of tuberculosis,leading to the request of the GeneXpert,which can detect Mycobacterium tuberculosis deoxyribonucleic acid and resistance to rifampicin.A decent diagnostic strategy is crucial to prevent unnecessary surgical resection and to supply conservative management with antitubercular therapy.

Improved edge lightweight YOLOv4 and its application in on-site power system work

A“cloud-edge-end”collaborative system architecture is adopted for real-time security management of power system on-site work,and mobile edge computing equipment utilizes lightweight intelligent recognition algorithms for on-site risk assessment and alert.Owing to its lightweight and fast speed,YOLOv4-Tiny is often deployed on edge computing equipment for real-time video stream detection;however,its accuracy is relatively low.This study proposes an improved YOLOv4-Tiny algorithm based on attention mechanism and optimized training methods,achieving higher accuracy without compromising the speed.Specifically,a convolution block attention module branch is added to the backbone network to enhance the feature extraction capability and an efficient channel attention mechanism is added in the neck network to improve feature utilization.Moreover,three optimized training methods:transfer learning,mosaic data augmentation,and label smoothing are used to improve the training effect of this improved algorithm.Finally,an edge computing equipment experimental platform equipped with an NVIDIA Jetson Xavier NX chip is established and the newly developed algorithm is tested on it.According to the results,the speed of the improved YOLOv4-Tiny algorithm in detecting on-site dress code compliance datasets is 17.25 FPS,and the mean average precision(mAP)is increased from 70.89%to 85.03%.

Random Forests Algorithm Based Duplicate Detection in On-Site Programming Big Data Environment

On-site programming big data refers to the massive data generated in the process of software development with the characteristics of real-time,complexity and high-difficulty for processing.Therefore,data cleaning is essential for on-site programming big data.Duplicate data detection is an important step in data cleaning,which can save storage resources and enhance data consistency.Due to the insufficiency in traditional Sorted Neighborhood Method(SNM)and the difficulty of high-dimensional data detection,an optimized algorithm based on random forests with the dynamic and adaptive window size is proposed.The efficiency of the algorithm can be elevated by improving the method of the key-selection,reducing dimension of data set and using an adaptive variable size sliding window.Experimental results show that the improved SNM algorithm exhibits better performance and achieve higher accuracy.

New Explorations of Laboratory and On-site Testing of Broadband Seismographs in China

According to different testing purposes, methods and available environmental conditions, the seismograph testing can be divided into laboratory and on-site testing, respectively. The testing of the seismograph's key parameters and other concerning technical specifications are well described in guide documents(China Earthquake Administration, 2017). This includes seismometer sensitivity, linearity and clip levels based on the shake table test, as well as the seismometer natural period, damping constant based on electrical calibration(Wang Guangfu,1986; Ple?inger A.,1993) and instrumental self-noise collocation estimation(Holcomb L.G., 1989; Sleeman R. et al., 2006). However, with the development of seismic observation technology, many new requirements for the performance evaluation of seismographs have been put forward, and new testing items and methods have emerged.

Dynamical energy equipartition of the Toda model with additional on-site potentials

We study the dynamical energy equipartition properties in the integrable Toda model with additional uniform or disordered on-site energies by extensive numerical simulations. The total energy is initially equidistributed among some of the lowest frequency linear modes. For the Toda model with uniform on-site potentials, the energy spectrum keeps its profile nearly unchanged in a relatively short time scale. On a much longer time scale, the energies of tail modes increase slowly with time. Energy equipartition is far away from being attached in our studied time scale. For the Toda model with disordered on-site potentials, the energy transfers continuously to the high frequency modes and eventually towards energy equipartition. We further perform a systematic study of the equipartition time teq depending on the energy density ε and the nonlinear parameter α in the thermodynamic limit for the Toda model with disordered on-site potentials. We find teq∝ （1/ε）^a（1/α）^b, where b≈ 2a. The values of a and b are increased when increasing the strengths of disordered on-site potentials or decreasing the number of initially excited modes.

Increased Effluent Dosage Effects on On-Site Wastewater Treatment Systems of Differing Architecture Type

Approximately 20% of homes nationwide use an on-site treatment system as a form of household wastewater management. However, approximately 10% to 20% of on-site treatment systems malfunction each year, many of which have either failed or exceeded the soil’s long-term acceptance rate (LTAR), causing environmental and human health risks. The objective of this field study was to evaluate the effects of soil condition (e.g., wet and dry) and product architecture type [i.e., chamber, gravel-less-pipe (GLP), polystyrene-aggregate, and pipe-and-aggregate] on in-product solution storage and biomat thickness in a profile-limited soil in northwest Arkansas under increased loading rates and to estimate the LTAR for each product. During Phase I of this study (March 13 to October 4, 2013), effluent loading rates were approximately doubled, while rates were approximately quadrupled during Phase II (October 8, 2013 to May 29, 2014), from the maximum allowable loading rate for each product. The pipe-and-tire-chip, 46-cm-wide trench pipe-and-gravel, and the 25-cm diameter GLP products had the greatest (p < 0.001), while the 31-cm-width and the 5.4-m-long chambers had the lowest (p < 0.001) in-product solution storage during wet-soil conditions of Phase I monitoring. The 25-cm diameter GLP product had the greatest (p < 0.001), while the 61-cm-width, 5.4-m-long chamber had the lowest (p < 0.001) in-product solution storage during Phase II. Results of this study indicate that some alternative products may be able to effectively handle effluent loading rates in excess of those currently allowed by the State of Arkansas. Further research will be required to confirm these interpretations.

On-Site Calibration Method of Dosimeter Based on X-Ray Source

The real-time monitoring of environmental radiation dose for nuclear fa-cilities is an important part of safety, in order to guarantee the accuracy of the monitoring results regular calibration is necessary. Around nuclear facilities there are so many environmental dosimeters installed dispers-edly, because of its huge quantity, widely distributed, and in real-time monitoring state;it will cost lots of manpower and finance if it were tak-en to calibrate on standard laboratory;what’s more it will make the en-vironment out of control. To solve the problem of the measurement ac-curacy of the stationary gamma radiation dosimeter, an on-site calibra-tion method is proposed. The radioactive source is X-ray spectrum, and the dose reference instrument which has been calibrated by the national standard laboratory is a high pressure ionization. On-site calibration is divided into two parts;firstly the energy response experiment of dosim-eter for high and low energy is done in the laboratory, and the energy response curve is obtained combining with Monte Carlo simulation;sec-ondly experiment is carried out in the field of the measuring dosimeter, and the substitution method to calibrate the dosimeter is used;finally the calibration coefficient is gotten through energy curve correction. In order to verify the accuracy of on-site calibration method, the calibrated dosimeter is test in the standard laboratory and the error is 3.4%. The re-sult shows that the on-site calibration method using X-ray is feasible, and it can improves the accuracy of the measurement results of the stationary &gamma;-ray instrument;what’s more important is that it has great reference value for the radiation safety management and radiation environment evaluation.

Simulation of fire emergency evacuation in a large-passenger-flow subway station based on the on-site measured data of Shenzhen Metro

Because of its large capacity,high efficiency and energy savings,the subway has gradually become the primary mode of transportation for citizens.A high density of passengers exists within a large-passenger-flow subway station,and the number of casualties and injuries during a fire emergency is substantial.In this paper,Pathfinder software and on-site measured data of Pingzhou station in Shenzhen(China)were utilized to simulate a fire emergency evacuation in a large-passenger-flow subway station.The Required Safe Egress Time(RSET),number of passengers and flow rates of stairs and escalators were analysed for three fire evacuation scenarios:train fire,platform fire and hall fire.The evacuation time of the train fire,which was 1173 s,was the longest,and 3621 occupants needed to evacuate when the train was fully loaded.Occupants could not complete the evacuation within 6 mins in all three fire evacuation scenarios,which does not meet the current standard requirements and codes.By changing the number of passengers and the number of stairs for evacuation,the flow rate capacity and evacuation time were explored,which have reference values for safety management and emergency evacuation plan optimization during peak hours of subway operation.

A critical review of on-site inorganic arsenic screening methods

Approximately 94 to 220 million people worldwide are at risk of drinking well water containing arsenic > 10 μg/L, the WHO guideline value. To identify non-compliant domestic wells, assess health risks and reduce exposure, accurate and rapid on-site inorganic arsenic screening methods are desirable because all domestic wells worldwide need to be tested.Here, the principles, advantages and limitations of commonly used colorimetry, electrochemistry, and biosensing methods are critically reviewed, with the performance compared with laboratory-based benchmark methods. Most commercial kits are based on the classic Gutzeit reaction. Despite being semi-quantitative, the more recent and more expensive products display improved and acceptable accuracy and shorter testing time (~10 min). Carried out by trained professionals, electrochemical methods are also feasible for on-site analysis, although miniaturization is desirable yet challenging. Biosensing using whole bacterial cells or bio-engineered materials such as aptamers is promising, if incorporated with function specific nanomaterials and biomaterials. Since arsenic is frequently found as arsenite in reducing groundwater and subject to oxidation during sampling, transportation and storage, on-site separation and sample preservation are feasible but the specific methods should be chosen based on sample matrix and tested before use. To eliminate arsenic exposure among hundreds of millions of mostly rural residents worldwide, we call for concerted efforts in research community and regulatory authority to develop accurate, rapid, and affordable tests for on-site screening and monitoring of arsenic in drinking water. Access to affordable testing will benefit people who are socioeconomically disadvantaged.

Supporting structure failure caused by the squeezing tunnel creep and its reinforcement measure

Tunnels deeply buried have high crustal stress and are prone to large deformation disasters when encountering soft rock.The large deformation phenomenon during the construction process of the Maoxian Tunnel on the Chengdu-Lanzhou Railway is particularly evident.This article focuses on the large deformation problem of the No.1 inclined shaft of the Maoxian Tunnel,and uses on-site monitoring methods to explore the reasons for tunnel structure failure,and analyzes the mechanical behavior of the tunnel structure.By using numerical simulation methods,the effectiveness of the second-layer support in resisting creep loads in tunnels was studied,and the influence of the construction time of the secondlayer support on the mechanical properties of the tunnel was discussed.The results indicate that the first-layer support in the tunnel is a structural failure caused by asymmetric deformation caused by creep,while the second-layer support has a good effect on resisting creep loads.The research results can provide a technical reference for deformation control of squeezing tunnels.

Soil erosion and its causes in high-filling body:A case study of a valley area on the Loess Plateau,China

Large-scale land consolidation projects(LCPs)have been carried out on the Loess Plateau to increase the area of agriculture land.The newly created land is prone to soil erosion under the effects of water and gravity.Taking a typical high-filling body(HFB)formed by LCPs in Yan’an,China as the subject,this study comprehensively investigated the types and causes of soil erosion with multiple methods of field investigation,on-site monitoring and laboratory tests.Results showed that the HFB presented a composite pattern of soil erosion with multiple types mainly including underground erosion,mixed water-gravity erosion,seepage erosion,and scouring erosion.The type of erosion varied spatially in different parts of the HFB depending on the dominant factors,mainly including the groundwater state,rainfall,runoff,gravity action,topography,and soil erodibility.The underground erosion mainly occurred at the positions with higher groundwater level and larger hydraulic gradient,while scouring erosion mainly occurred at the positions with extensive interactions of surface runoff,channel slope gradient and soil properties.And near the leading edge of the top of the slope,a band of mixed watergravity erosion occurred owing to the effects of water and gravity.In addition,nearly saturated soils at the toe of HFB displayed groundwater exfiltration and slope-face slumping.Based on our findings on the causes and variation of soil erosion for the HFB,we proposed the following erosion prevention and control measures to protect the LCPs on the Loess Plateau:to construct drainage ditches and blind ditches to form a complete drainage system,plant alfalfa on the top platform to increase rainfall interception and reduce surface runoff,set seepage ditches and plant deep-rooted plants at the toe of the slope to improve slope toe stability,monitor groundwater level and slope deformation to learn the erosion dynamics and slope stability,and optimize the geometry of HFB such as the slope gradient and slope steps to reduce soil erosion.

Performance of the suspension method in large cross-section shallow-buried tunnels

Large cross-section tunnel construction induces ground surface settlements, potentially endangering both subterranean projects and nearby above-ground structures. A novel tunnel construction method, known as the suspension method,is introduced in this paper to mitigate surface settlement. The suspension method employs vertical tie rods to establish a structural connection between the initial tunnel support system and the surface steel beam, thereby exerting effective control settlements. To analyze the performance of the proposed method, systematic numerical simulations were conducted based on the practical engineering of Harbin Subway Line 3. The surface settlement and vault settlement characteristics during construction are investigated. The results show a gradual increment in both surface and vault settlement throughout the construction process, culminating in a stabilized state upon the completion of construction.In addition, compared to the double-side drift method and the Cross Diaphragm Method(CRD) method, the suspension method can obviously reduce the surface settlement and vault settlement. Moreover, the surface settlements and the axial force of tie rods were continuously monitored during the construction process at the trial tunnel block.These specific monitoring measurements are illustrated in comparison to numerical analysis results. The monitored results show great agreement with the numerical predictions, confirming the success of the project. This research can serve as a valuable practical reference for similar projects, offering insights and guidance for addressing ground surface settlements and enhancing construction safety in the domain of large cross-section tunneling.

Real-time prediction of earthquake potential damage:A case study for the January 8,2022 M_(S) 6.9 Menyuan earthquake in Qinghai,China

It is critical to determine whether a site has potential damage in real-time after an earthquake occurs,which is a challenge in earthquake disaster reduction.Here,we propose a real-time Earthquake Potential Damage predictor(EPDor)based on predicting peak ground velocities(PGVs)of sites.The EPDor is composed of three parts:(1)predicting the magnitude of an earthquake and PGVs of triggered stations based on the machine learning prediction models;(2)predicting the PGVs at distant sites based on the empirical ground motion prediction equation;(3)generating the PGV map through predicting the PGV of each grid point based on an interpolation process of weighted average based on the predicted values in(1)and(2).We apply the EPDor to the 2022 M_(S) 6.9 Menyuan earthquake in Qinghai Province,China to predict its potential damage.Within the initial few seconds after the first station is triggered,the EPDor can determine directly whether there is potential damage for some sites to a certain degree.Hence,we infer that the EPDor has potential application for future earthquakes.Meanwhile,it also has potential in Chinese earthquake early warning system.

Study on wear characteristics and authorized limits of switch rails of high-speed turnout

Purpose-It is quite universal for high-speed turnouts to be exposed to the wear of the stock rail of the switch rail during the service process.The wear will cause the change of railhead profile and the relative positions of the switch rail and the stock rail,which will directly affect the wheel-rail contact state and wheel load transition when a train passes the turnout and will further impose serious impacts on the safety and stability of train operation.The purpose of this paper is to provide suggestions for wear management of high-speed turnout.Design/methodology/approach-The actual wear characteristics of switch rails of high-speed turnouts in different guiding directions were studied based on the monitoring results on site;the authorized wear limits for the switch rails of high-speed turnout were studied through derailment risk analysis and switch rail strength analysis.Findings-The results show that:the major factor for the service life of a curved switch rail is the lateral wear.The wear characteristics of the curved switch rail of a facing turnout are significantly different from those of a trailing turnout.To be specific,the lateral wear of the curved switch rail mainly occurs in the narrower section at its front end for a trailing turnout,but in the wider section at its rear end when for a facing turnout.The maximum lateral wear of a dismounted switch rail from a trailing turnout is found on the 15-mm wide section and is 3.9 mm,which does not reach the specified limit of 6 mm.For comparison,the lateral wear of a dismounted switch rail from a facing turnout is found from the 35-mm wide section to the full-width section and is greater than 7.5 mm,which exceeds the specified limit.Based on this,in addition to meeting the requirements of maintenance rules,the allowed wear of switch rails of high-speed turnout shall be so that the dangerous area with a tangent angle of wheel profile smaller than 43.68 will not contact the switch rail when the wheel is lifted by 2 mm.Accordingly,the lateral wear limit at the 5-mm wide section of the curved switch rail shall be reduced from 6 mm(as specified)to 3.5 mm.Originality/value-The work in this paper is of reference significance to the research on the development law of rail wear in high-speed turnout area and the formulation of relevant standards.

Stimulatory effects of biosurfactant produced by Pseudomonas aeruginosa BSZ-07 on rice straw decomposing

Biosurfactant,produced by Pseudomonas aeruginosa BSZ-07,was added to the dee straw decomposing process to enhance the production of reducing sugars.Observed by Fourier Transform InfraRed(FT-IR)and Nuclear Magnetic Resonance(NMR)analysis, the purified biosurfactant was considered as a mixture of RL1 and RL2,which are two different types of rhamnolipids.Two different adding methods,adding the purified rhamnolipid and the on-site production of it were compared.The results showed that 0.5 g/L was the optimum co...

Overburden management in open pits: options and limits in large limestone quarries

The management of overburden is an important task in open pit exploitations. Site topography and morphology as well as geological and geotechnical properties of natural and remoulded materials are the most important factors affecting the disposal phase. Economic and environmental requirements must be followed in order to achieve the best reclamation results, keeping into account site constraints such as slope stability, hauling and dumping issues, and interactions with groundwater. This paper deals with the above mentioned issues, illustrating a rational approach applied on the case of a large limestone quarry where the thickness of the overburden is relevant and the spoil material has to be dumped in a flooded pit. The proposed multidisciplinary approach led to the selection of most suitable methods for excavation, transportation and disposal. The selection was based on a detailed laboratory and site characterisation that defined favorable and adverse factors to be considered during the preliminary study of a large quarrying project.

A Quantitative Evaluation of Shale Gas Content in Different Occurrence States of the Longmaxi Formation: A New Insight from Well JY-A in the Fuling Shale Gas Field,Sichuan Basin

Comprehensive quantitative evaluation of shale gas content and the controlling factors in different occurrence states is of great significance for accurately assessing gas-bearing capacity and providing effective well-production strategies. A total of 122 core samples from well JY-A in the Fuling shale gas field were studied to reveal the characteristics of S_1 l shale,15 of which were selected to further predict the shale gas content in different occurrence states, which are dependent on geological factors in the thermal evolution process. Geological parameters were researched by a number of laboratory programs, and the factors influential in controlling shale gas content were extracted by both PCA and GRA methods and prediction models were confirmed by the BE method using SPSS software. Results reveal that the adsorbed gas content is mainly controlled by TOC, Ro, SSA, PD and pyrite content, and the free gas content is mainly controlled by S_2, quartz content, gas saturation and formation pressure for S_1 l in well JY-A. Three methods, including the on-site gas desorption method, the empirical formula method, and the multiple regression analysis method were used in combination to evaluate the shale gas capacity of well JY-A, all of which show that the overall shale gas content of well JY-A is in the range of 2.0–5.0 m^3/t and that the free gas ratio is about 50%, lower than that of well JY-1. Cause analysis further confirms the tectonics and preservation conditions of S_1 l in the geological processes, especially the influence of eastern boundary faults on well JY-A, as the fundamental reasons for the differences in shale gas enrichment in the Jiaoshiba area.

Formation and emission characteristics of VOCs from a coal-fired power plant

On-site measurements of volatile organic compounds(VOCs)in different streams of flue gas were carried out on a real coal-fired power plant using sampling bags and SUMMA canisters to collect gas samples,filters to collect particle samples.Gas chromatography-flame ionization detector/mass spectrometry and gas chromatography-mass spectrometry was the offline analysis method.We found that the total mass concentration of the tested 102 VOC species at the outlet of wet flue gas desulfuration device was(13456±47)μg·m^(-3),which contained aliphatic hydrocarbons(57.9%),aromatic hydrocarbons(26.8%),halogen-containing species(14.5%),and a small amount of oxygen-containing and nitrogencontaining species.The most abundant species were 1-hexene,n-hexane and 2-methylpentane.The top ten species in terms of mass fraction(with a total mass fraction of 75.3%)were mainly hydrocarbons with a carbon number of 6 or higher and halogenated hydrocarbons with a lower carbon number.The mass concentration of VOC species in the particle phase was significantly lower than that in the gas phase.The change of VOC mass concentrations along the air pollution control devices indicates that conventional pollutant control equipment had a limited effect on VOC reduction.Ozone formation potential calculations showed that aromatic hydrocarbons contributed the highest ozone formation(46.4%)due to their relatively high mass concentrations and MIR(maximum increment reactivity)values.

Investigation of the gas bubble dynamics induced by an electric arc in insulation oil

In this work,experimental and theoretical studies were carried out on arc-induced bubble dynamic behaviors in insulation oil.Direct experimental evidence indicated that the arc-induced bubble experiences pulsating growth rather than a continuous expansion.Furthermore,a theoretical model and numerical calculation method were proposed,which revealed the dynamic mechanism of bubble growth.Good agreement between the theoretical results and experimental observations verified the general correctness and feasibility of the proposed method.