The aim of this study was to provide a basis for examining the molecular mechanism for the pharmacological action of ethanol. We studied di?myristoyl?phosphatidylethanol (DMPEt)’s effects on specific locations of n-(...The aim of this study was to provide a basis for examining the molecular mechanism for the pharmacological action of ethanol. We studied di?myristoyl?phosphatidylethanol (DMPEt)’s effects on specific locations of n-(9-anthroyloxy) palmitic acid or stearic acid (n-AS) within phos?pholipids of synaptosomal plasma membrane vesicles isolated from bovine cerebral cortex (SPMV) and liposomes of total lipids (SPMVTL) and phospholipids (SPMVPL) extracted from SPMV. DMPEt increased rotational mobility (increased disordering) of hydrocarbon interior, but it decreased mobility (increased ordering) of membrane interface, in native and model membranes. The degree of rotational mobility in accordance with the carbon atom numbers of phospholipids comprising neuronal membranes was in the order at the 16, 12, 9, 6 and 2 position of aliphatic chain present in phospholipids. The sensitivity of increasing or decreasing effect of rotational mobility of hydrocarbon interior or surface region by DMPEt differed depending on the neuronal and model membranes in the descending order of SPMV, SPMVPL and SPMVTL.展开更多
Fabrication of anisotropic material is one of the important topics and we examined to introduce “anisotropic” nature by spreading polymer-grafted particle on the medium with polymer-reactive potential. Poly (tert-bu...Fabrication of anisotropic material is one of the important topics and we examined to introduce “anisotropic” nature by spreading polymer-grafted particle on the medium with polymer-reactive potential. Poly (tert-butyl methacrylate) (PtBMA) was polymerized from polystyrene latex (PSL) surface by ATRP to give PtBMA-grafted PSL (PSL-PtBMA). Particle monolayer was formed at air-water and air-acidic water interfaces and the monolayer characteristics were compared by π-A isotherm measurements, SEM observations, and contact angle measurements. π-A isotherms, in particular, indicates that the interaction between polymer chains become stronger by changing the subphase condition, which suggests that anisotropicparticle monolayer formation.展开更多
文摘The aim of this study was to provide a basis for examining the molecular mechanism for the pharmacological action of ethanol. We studied di?myristoyl?phosphatidylethanol (DMPEt)’s effects on specific locations of n-(9-anthroyloxy) palmitic acid or stearic acid (n-AS) within phos?pholipids of synaptosomal plasma membrane vesicles isolated from bovine cerebral cortex (SPMV) and liposomes of total lipids (SPMVTL) and phospholipids (SPMVPL) extracted from SPMV. DMPEt increased rotational mobility (increased disordering) of hydrocarbon interior, but it decreased mobility (increased ordering) of membrane interface, in native and model membranes. The degree of rotational mobility in accordance with the carbon atom numbers of phospholipids comprising neuronal membranes was in the order at the 16, 12, 9, 6 and 2 position of aliphatic chain present in phospholipids. The sensitivity of increasing or decreasing effect of rotational mobility of hydrocarbon interior or surface region by DMPEt differed depending on the neuronal and model membranes in the descending order of SPMV, SPMVPL and SPMVTL.
基金This work was financially supported by a grant-in-aid(No.19750099,24850015)from JSPS.
文摘Fabrication of anisotropic material is one of the important topics and we examined to introduce “anisotropic” nature by spreading polymer-grafted particle on the medium with polymer-reactive potential. Poly (tert-butyl methacrylate) (PtBMA) was polymerized from polystyrene latex (PSL) surface by ATRP to give PtBMA-grafted PSL (PSL-PtBMA). Particle monolayer was formed at air-water and air-acidic water interfaces and the monolayer characteristics were compared by π-A isotherm measurements, SEM observations, and contact angle measurements. π-A isotherms, in particular, indicates that the interaction between polymer chains become stronger by changing the subphase condition, which suggests that anisotropicparticle monolayer formation.
