Leaf is a vital organ of plants that plays an essential role in photosynthesis and respiration.As an important agronomic trait in leaf development,leaf shape is classified into lobed,entire(no-lobed),and serrated in m...Leaf is a vital organ of plants that plays an essential role in photosynthesis and respiration.As an important agronomic trait in leaf development,leaf shape is classified into lobed,entire(no-lobed),and serrated in most crops.In this study,two-lobed leaf watermelon inbred lines WT2 and WCZ,and a no-lobed leaf watermelon inbred line WT20 were used to create two F_(2)populations.Segregation analysis suggested that lobed leaves were dominant over the no-lobed leaves,and it was controlled by a signal gene.A locus on watermelon chromosome 4 controlling watermelon lobed/no-lobed leaves was identified through BSA-seq strategy combined with linkage analysis.The candidate gene was fine-mapped to a 61.5 kb region between 21,224,481 and 21,285,957 bp on watermelon chromosome 4 using two F_(2)populations.Four functional genes were annotated in the candidate region,while sequences blast showed that there was a single-base deletion(A/-)only in the exon of Cla018360,which resulted in premature termination of translation in the no-lobed leaf lines.Function prediction showed that Cla018360 encodes an HD-Zip protein that has been reported to regulate the development of leaf shape.The single-base deletion also occurred in the HD-Zip domain.We inferred that the Cla018360 gene is the candidate gene for regulating the development of lobed/no-lobed leaves in watermelon.Gene expression analysis showed that Cla018360 was highly expressed in young leaves.Phylogenetic analysis showed that Cla018360 had a close genetic relationship with AtHB51,which had been reported to regulate the formation of leaf shape in Arabidopsis.Furthermore,transcriptome analysis showed that a total of 333 differentially expressed genes were identified between WT2 and WT20,of which 115 and 218 genes were upregulated and downregulated in no-lobed leaved watermelon WT20.This study not only provides a good entry point for studying leaf development but also provides foundational insights into breeding for special plant architecture in watermelon.展开更多
Partial epilepsies, originating in a specific brain region, affect about 60% of adults with epilepsy. Temporal lobe epilepsy (TLE) is the most prevalent subtype within this category, often necessitating surgical inter...Partial epilepsies, originating in a specific brain region, affect about 60% of adults with epilepsy. Temporal lobe epilepsy (TLE) is the most prevalent subtype within this category, often necessitating surgical intervention due to its refractoriness to antiepileptic drugs (AEDs). Hippocampal sclerosis, a common underlying pathology, often exacerbates the severity by introducing cognitive and emotional challenges. This review delves deeper into the cognitive profile of TLE, along with the risk factors for cognitive disorders, depression, and anxiety in this population.展开更多
The performance of a newly designed tri-lobe industrial lobe pump of high capacity is simulated by using commercial CFD solver Ansys Fluent. A combination of user-defined-functions and meshing strategies is employed t...The performance of a newly designed tri-lobe industrial lobe pump of high capacity is simulated by using commercial CFD solver Ansys Fluent. A combination of user-defined-functions and meshing strategies is employed to capture the rotation of the lobes. The numerical model is validated by comparing the simulated results with the literature values. The processes of suction, displacement, compression and exhaust are accurately captured in the transient simulation. The fluid pressure value remains in the range of inlet pressure value till the processes of suction and displacement are over. The instantaneous process of compression is accurately captured in the simulation. The movement of a particular working chamber is traced along the gradual degree of lobe’s rotation. At five different degrees of lobe’s rotation, pressure contour plots are reported which clearly shows the pressure values inside the working chamber. Each pressure value inside the working chamber conforms to the particular process in which the working chamber is operating. Finally, the power requirement at the shaft of rotation is estimated from the simulated values. The estimated value of power requirement is 3.61 BHP FHP whereas the same calculated theoretically is 3 BHP FHP. The discrepancy is attributed to the assumption of symmetry of blower along the thickness.展开更多
BACKGROUND Gelastic seizure(GS)is a rare type of epilepsy that most commonly appears in patients with hypothalamic hamartoma.It is rarely associated with other types of brain damage.This particular type of epilepsy is...BACKGROUND Gelastic seizure(GS)is a rare type of epilepsy that most commonly appears in patients with hypothalamic hamartoma.It is rarely associated with other types of brain damage.This particular type of epilepsy is relatively rare and has few links to other brain lesions.Temporal lobe malacia is mostly caused by cerebral infarction or cerebral hemorrhage,which can lead to seizures.We report a case of GS in a woman with temporal lobe malacia which was reported for the first time in the literature.CASE SUMMARY A 73-year-old female,diagnosed case of GS,presented with repetitive stereotyped laughter a month prior to presentation,happening multiple times daily and with each time lasting for 5-15s.Electroencephalogram displayed a focal seizure seen in the right temporal region.Magnetic resonance imaging head with contrast showed a right temporal lobe malacia.The patient was started on levetiracetam daily.The patient indicated that they had fully recovered and were not experiencing any recurrent or stereotyped laughter during their daily routines.These results remained consistent even after a one-year follow-up period.CONCLUSION GS can be caused by temporal lobe malacia,which is an uncommon but potentially grave condition.The outcome of this present case exhibited the importance of the temporal lobe in the genesis of GS.展开更多
Temporal lobe epilepsy is a multifactorial neurological dysfunction syndrome that is refractory,resistant to antiepileptic drugs,and has a high recurrence rate.The pathogenesis of temporal lobe epilepsy is complex and...Temporal lobe epilepsy is a multifactorial neurological dysfunction syndrome that is refractory,resistant to antiepileptic drugs,and has a high recurrence rate.The pathogenesis of temporal lobe epilepsy is complex and is not fully understood.Intracellular calcium dynamics have been implicated in temporal lobe epilepsy.However,the effect of fluctuating calcium activity in CA1 pyramidal neurons on temporal lobe epilepsy is unknown,and no longitudinal studies have investigated calcium activity in pyramidal neurons in the hippocampal CA1 and primary motor cortex M1 of freely moving mice.In this study,we used a multichannel fiber photometry system to continuously record calcium signals in CA1 and M1 during the temporal lobe epilepsy process.We found that calcium signals varied according to the grade of temporal lobe epilepsy episodes.In particular,cortical spreading depression,which has recently been frequently used to represent the continuously and substantially increased calcium signals,was found to correspond to complex and severe behavioral characteristics of temporal lobe epilepsy ranging from gradeⅡto gradeⅤ.However,vigorous calcium oscillations and highly synchronized calcium signals in CA1 and M1 were strongly related to convulsive motor seizures.Chemogenetic inhibition of pyramidal neurons in CA1 significantly attenuated the amplitudes of the calcium signals corresponding to gradeⅠepisodes.In addition,the latency of cortical spreading depression was prolonged,and the above-mentioned abnormal calcium signals in CA1 and M1 were also significantly reduced.Intriguingly,it was possible to rescue the altered intracellular calcium dynamics.Via simultaneous analysis of calcium signals and epileptic behaviors,we found that the progression of temporal lobe epilepsy was alleviated when specific calcium signals were reduced,and that the end-point behaviors of temporal lobe epilepsy were improved.Our results indicate that the calcium dynamic between CA1 and M1 may reflect specific epileptic behaviors corresponding to different grades.Furthermore,the selective regulation of abnormal calcium signals in CA1 pyramidal neurons appears to effectively alleviate temporal lobe epilepsy,thereby providing a potential molecular mechanism for a new temporal lobe epilepsy diagnosis and treatment strategy.展开更多
BACKGROUND In laparoscopic proximal gastrectomy(LPG),the prolapse of the hepatic left lateral lobe near the lesser curvature and esophageal hiatus can obstruct the field of vision and operation.Therefore,it is necessa...BACKGROUND In laparoscopic proximal gastrectomy(LPG),the prolapse of the hepatic left lateral lobe near the lesser curvature and esophageal hiatus can obstruct the field of vision and operation.Therefore,it is necessary to retract or obstruct the hepatic left lateral lobe to ensure a clear field of vision.AIM To investigate the safety and clinical efficacy of the modified hepatic left lateral lobe inversion technique for LPG.METHODS A retrospective analysis was conducted on the clinical data of 13 consecutive patients with early-stage upper gastric adenocarcinoma or adenocarcinoma of the esophagogastric junction treated with LPG from January to December 2023 at the Department of Gastrointestinal Surgery,Second Affiliated Hospital of Fujian Medical University.The modified hepatic left lateral lobe inversion technique was used to expose the surgical field in all patients,and short-term outcomes were observed.RESULTS In all 13 patients,the modified hepatic left lateral lobe inversion technique was successful during surgery without the need for re-retraction or alteration of the liver traction method.There were no instances of esophageal hiatus occlusion,eliminating the need for forceps to assist in exposure.There was no occurrence of intraoperative hepatic hemorrhage,hepatic vein injury,or hepatic congestion.No postoperative digestive complications of Clavien-Dindo grade≥II occurred wi-thin 30 days after surgery,except for a single case of pulmonary infection.Some patients experienced increases in alanine aminotransferase and aspartate aminotransferase levels on the first day after surgery,which significantly decreased by the third day and returned to normal by the seventh day after surgery.CONCLUSION The modified hepatic left lateral lobe inversion technique has demonstrated satisfactory results,offering ad-vantages in terms of facilitating surgical procedures,reducing surgical trauma,and protecting the liver.展开更多
The flow field and aerodynamic performances for the scarfed lobed forced mixer are studied based on a computational fluid dynamics(CFD) technique. A series of computations are conducted to obtain the effects of the ...The flow field and aerodynamic performances for the scarfed lobed forced mixer are studied based on a computational fluid dynamics(CFD) technique. A series of computations are conducted to obtain the effects of the bypass ratio and the scarf angle on the mixing performance for the scarfed lobed mixer. Results show that the scarfed lobed mixer is reduced in the system weight. Meanwhile, aerodynamic performances are slightly improved compared with the normal lobed mixer. Two reasons for causing the mixing enhancement between the core and the bypass flow are as follows: (1) The stream-wise vortices shed from the training edge of the half/full scarfed lobed mixer earlier is enhanced by about 25%. (2) The mixing augmentation is also associated with the increase of the interface length caused by scarfing. The thermal mixing efficiency is enhanced with the increase of the bypass ratio and the scarfing angle. The scarfed lobed mixer design has no negative effects on the pressure loss. The total pressure recovery coefficient reaches above 0. 935 in various bypass ratios and scarfed angles. As the bypass ratio increases, the total pressure recovery coefficient also increases for the scarfed lobed mixer.展开更多
[Objective] We aimed to investigate the preventive and therapeutical effect of compound of traditional Chinese drugs (Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root) on acute alc...[Objective] We aimed to investigate the preventive and therapeutical effect of compound of traditional Chinese drugs (Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root) on acute alcohol intoxication in mice. [Method] Acute alcohol intoxication was induced by administering alcohol to mice. Three different doses (low, middle and high) of compound of traditional Chinese drugs were administered to mice before and after administering alcohol respectively to investigate the preventive and therapeutical effect of drugs on acute alcohol intox-ication through doing statistical analysis about drunk mice and their sleeping time. The concentration of malondialdehyde (MDA), reduced glutathione (GSH) and triglyc-erides (TG) in liver was also determined to investigate the protective effect of drugs on liver. [Result] The efficacy of compound of traditional Chinese drugs on acute al-cohol intoxication was dose-dependent. High-dose administration decreased the number of drunk mice significantly compared with control group; middle- and high-dose administration reduced the sleeping time of drunk mice and the concentration of MDA and TG in liver tissue; three doses al increased the concentration of GSH. [Conclusion] The compound of Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root had preventive and therapeutical effect on hangover, and it also had certain preventive and therapeutical effect on liver damage caused by alcohol.展开更多
Three-dimensional numerical computation of the flow fields and pumping performances for the lobed mixer-ejector are conducted using full Navier-Stokes equations. In the computation, the inlet of the primary flow uses ...Three-dimensional numerical computation of the flow fields and pumping performances for the lobed mixer-ejector are conducted using full Navier-Stokes equations. In the computation, the inlet of the primary flow uses the mass flowrate boundary condition. The inlet of the second flow and the outlet of the mixing flow use the pressure boundary condition. Compared with the relative experimental resuits, it is shown that the present calculation is reasonable. And a series of numerical studies is performed to obtain the effects of area ratio and length-to-diameter ratio of mixing duct on pumping coefficient and thermal mixing efficiency of a lobed mixer-ejector.展开更多
Temporal lobe epilepsy is the most common form of focal epilepsy in adults,accounting for one third of all diagnosed epileptic patients,with seizures originating from or involving mesial temporal structures such as th...Temporal lobe epilepsy is the most common form of focal epilepsy in adults,accounting for one third of all diagnosed epileptic patients,with seizures originating from or involving mesial temporal structures such as the hippocampus,and many of these patients being refractory to treatment with anti-epileptic drugs.Temporal lobe epilepsy is the most common childhood neurological disorder and,compared with adults,the symptoms are greatly affected by age and brain development.Diagnosis of temporal lobe epilepsy relies on clinical examination,patient history,electroencephalographic recordings,and brain imaging.Misdiagnosis or delay in diagnosis is common.A molecular biomarker that could distinguish epilepsy from healthy subjects and other neurological conditions would allow for an earlier and more accurate diagnosis and appropriate treatment to be initiated.Among possible biomarkers of pathological changes as well as potential therapeutic targets in the epileptic brain are micro RNAs.Most of the recent studies had performed micro RNA profiling in body fluids such as blood plasma and blood serum and brain tissues such as temporal cortex tissue and hippocampal tissue.A large number of micro RNAs were dysregulated when compared to healthy controls and with some overlap between individual studies that could serve as potential biomarkers.For example,in adults with temporal lobe epilepsy,possible biomarkers are miR-199a-3p in blood plasma and miR-142-5p in blood plasma and blood serum.In adults with mesial temporal lobe epilepsy,possible biomarkers are miR-153 in blood plasma and miR-145-3p in blood serum.However,in many of the studies involving patients who receive one or several anti-epileptic drugs,the influence of these on micro RNA expression in body fluids and brain tissues is largely unknown.Further studies are warranted with children with temporal lobe epilepsy and consideration should be given to utilizing mouse or rat and non-human primate models of temporal lobe epilepsy.The animal models could be used to confirm micro RNA findings in human patients and to test the effects of targeting specific micro RNAs on disease progression and behavior.展开更多
An experimental study was conducted to investigate the evolutions of unsteady vortex structures downstream a lobed mixer/nozzle.A novel dual-plane stereoscopic PIV system was used to measure all 3-components of vortic...An experimental study was conducted to investigate the evolutions of unsteady vortex structures downstream a lobed mixer/nozzle.A novel dual-plane stereoscopic PIV system was used to measure all 3-components of vorticity distributions to revealed both the large-scale streamwise vortices produced by the lobed mixer/nozzle and the Kelvin-Helmholtz vortex structures generated due to the Kelvin-Helmholtz instabilities simultaneously and quantitatively for the first time.The instantaneous and the ensemble-averaged vorticity distributions displayed quite different aspects about the evolutions of the unsteady vortex structures.While the ensemble-averaged vorticity distributions indicated the overall effect of the special geometry of the lobed nozzle/mixer on the enhanced mixing process,the instantaneous vorticity distributions elucidated many details about how the enhanced mixing process was conducted.In addition to quantitatively confirming conjectures of previous studies,further insight about the formation,evolution and interaction characteristics of the unsteady vortex structures downstream of the lobed mixer/nozzle were also uncovered quantitatively in the present study.展开更多
Background: Improving the mechanical properties and angiogenesis of acellular scaffolds before transplantation is an important challenge facing the development of acellular liver grafts. The present study aimed to eva...Background: Improving the mechanical properties and angiogenesis of acellular scaffolds before transplantation is an important challenge facing the development of acellular liver grafts. The present study aimed to evaluate the cytotoxicity and angiogenesis of polyethylene glycol(PEG) crosslinked decellularized single liver lobe scaffolds(DLSs), and establish its suitability as a graft for long-term liver tissue engineering. Methods: Using mercaptoacrylate produced by the Michael addition reaction, DLSs were first modified using N-succinimidyl S-acetylthioacetate(SATA), followed by cross-linking with PEG as well as vascular endothelial growth factor(VEGF). The optimal concentration of agents and time of the individual steps were identified in this procedure through biomechanical testing and morphological analysis. Subsequently, human umbilical vein endothelial cells(HUVECs) were seeded on the PEG crosslinked scaffolds to detect the proliferation and viability of cells. The scaffolds were then transplanted into the subcutaneous tissue of Sprague-Dawley rats to evaluate angiogenesis. In addition, the average number of blood vessels was evaluated in the grafts with or without PEG at days 7, 14, and 21 after implantation. Results: The PEG crosslinked DLS maintained their three-dimensional structure and were more translucent after decellularization than native DLS, which presented a denser and more porous network structure. The results for Young’s modulus proved that the mechanical properties of 0.5 PEG crosslinked DLS were the best and close to that of native livers. The PEG-VEGF-DLS could better promote cell proliferation and differentiation of HUVECs compared with the groups without PEG cross-linking. Importantly, the average density of blood vessels was higher in the PEG-VEGF-DLS than that in other groups at days 7, 14, and 21 after implantation in vivo. Conclusions: The PEG crosslinked DLS with VEGF could improve the biomechanical properties of native DLS, and most importantly, their lack of cytotoxicity provides a new route to promote the proliferation of cells in vitro and angiogenesis in vivo in liver tissue engineering.展开更多
In this research work,we present the evolution of Coulomb failure stress(CFS)in the Sulaiman Lobe and its implications for seismic hazard assessment.The Chaman transform fault,~1,000 km long,is the major active fault ...In this research work,we present the evolution of Coulomb failure stress(CFS)in the Sulaiman Lobe and its implications for seismic hazard assessment.The Chaman transform fault,~1,000 km long,is the major active fault that marks the western boundary between Pakistan and Afghanistan on the Indian Plate.To date,few studies have been conducted to unveil the interactions among earthquakes and the implications of these interactions for seismic hazard assessment in the region.We thoroughly investigated the published and online catalog to construct a sequence of major earthquakes that occurred in this region during the past.The final earthquake sequence was composed of 15 earthquakes of M_(w)≥6.0,beginning with the 1888 earthquake.We used the stress-triggering theory to numerically simulate the evolution of CFS caused by these earthquakes.The numerical results revealed that 8 out of 15earthquakes were triggered by the preceding earthquakes.The earthquakes in 1908,1910,1935,1966,and 1997 were rather independent earthquakes in this sequence.Although the epicenters of the 1975a and 1975b earthquakes were in the stress shadow zone,the partial rupture segments of both these earthquakes were in high-CFS regions.The CFS induced by the 1935 earthquake was notable,as it later triggered the 2008 doublet.Moreover,our results revealed that the northern segment of the Chaman Fault,the southern segment of the Ghazaband Fault,and the northwestern segment of the Urghargai Fault demonstrated a high change in CFS that could trigger seismicity in these regions.The necessary arrangements must therefore be made to mitigate any possible seismic hazards in the region.展开更多
In order to investigate the effect of the radial gradation of the lobed nozzles on the flow field organization,a cold water model experimental platform for a combustion chamber with radial⁃staged 13-point lobed nozzle...In order to investigate the effect of the radial gradation of the lobed nozzles on the flow field organization,a cold water model experimental platform for a combustion chamber with radial⁃staged 13-point lobed nozzles is built.Compared with a series of combustion OH*luminescence experiments tested by the University of Cincinnati,the four corresponding working conditions of no load,partial load,cruise and take off are selected.The vortex structure,vorticity value,multi-combustion materiel field and combustion characteristics of the flow field in the radial staged combustion chamber of the lobed nozzles under the equivalence ratio,the fuel injection method,the fuel injection ratio and other factors are numerically studied.The results show that under different influencing factors,the varation trend of the hydroxyl flame field of the lobe combustion chamber is basically the same as that of the hydroxyl light emission experiment of the swirl combustion chamber,but the flame field shape is quite different.The local equivalent ratio has a greater influence on the relevant combustion performance of the combustion chamber.Under the conditions of lower equivalence ratio,three-stage air and fuel injection mode,and gradually transferring the fuel flow of the pilot circuit to the external circuit,the temperature field and flame field of the combustion chamber are more evenly distributed,the outlet temperature field quality is better,the combustion efficiency is higher,and the NO_(X) emission is relatively low.These are basically consistent with the cold test results.The cold experimental results illustrate the importance of the influence of the flow field organization on the combustion organization and verify the reliability of the calculation results.展开更多
Cognitive impairment is the most common complication in patients with temporal lobe epilepsy with hippocampal scle rosis.There is no effective treatment for cognitive impairment.Medial septum cholinergic neurons have ...Cognitive impairment is the most common complication in patients with temporal lobe epilepsy with hippocampal scle rosis.There is no effective treatment for cognitive impairment.Medial septum cholinergic neurons have been reported to be a potential target for controlling epileptic seizures in tempo ral lobe epile psy.However,their role in the cognitive impairment of temporal lobe epilepsy remains unclear.In this study,we found that patients with temporal lobe epile psy with hippocampal sclerosis had a low memory quotient and severe impairment in verbal memory,but had no impairment in nonverbal memory.The cognitive impairment was slightly correlated with reduced medial septum volume and medial septum-hippocampus tra cts measured by diffusion tensor imaging.In a mouse model of chronic temporal lobe epilepsy induced by kainic acid,the number of medial septum choline rgic neurons was reduced and acetylcholine release was reduced in the hippocampus.Furthermore,selective apoptosis of medial septum cholinergic neurons mimicked the cognitive deficits in epileptic mice,and activation of medial septum cholinergic neurons enhanced hippocampal acetylcholine release and restored cognitive function in both kainic acid-and kindling-induced epile psy models.These res ults suggest that activation of medial septum cholinergic neurons reduces cognitive deficits in temporal lobe epilepsy by increasing acetylcholine release via projections to the hippocampus.展开更多
基金This research was supported by the National Natural Science Foundation of China(32102389,32172602)the Zhongyuan Youth Talent Support Program(ZYQR201912161)+3 种基金the Program for Science&Technology Innovation Talents in Universities of Henan Province(21HASTIT038)the Funding of Joint Research on Agricultural Varietie Improvement of Henan Province(2022010503)the Major Science and Technology Project of Henan Province(221100110400)the Science and Technology Innovation Fund of Henan Agricultural University(KJCX2021A14).
文摘Leaf is a vital organ of plants that plays an essential role in photosynthesis and respiration.As an important agronomic trait in leaf development,leaf shape is classified into lobed,entire(no-lobed),and serrated in most crops.In this study,two-lobed leaf watermelon inbred lines WT2 and WCZ,and a no-lobed leaf watermelon inbred line WT20 were used to create two F_(2)populations.Segregation analysis suggested that lobed leaves were dominant over the no-lobed leaves,and it was controlled by a signal gene.A locus on watermelon chromosome 4 controlling watermelon lobed/no-lobed leaves was identified through BSA-seq strategy combined with linkage analysis.The candidate gene was fine-mapped to a 61.5 kb region between 21,224,481 and 21,285,957 bp on watermelon chromosome 4 using two F_(2)populations.Four functional genes were annotated in the candidate region,while sequences blast showed that there was a single-base deletion(A/-)only in the exon of Cla018360,which resulted in premature termination of translation in the no-lobed leaf lines.Function prediction showed that Cla018360 encodes an HD-Zip protein that has been reported to regulate the development of leaf shape.The single-base deletion also occurred in the HD-Zip domain.We inferred that the Cla018360 gene is the candidate gene for regulating the development of lobed/no-lobed leaves in watermelon.Gene expression analysis showed that Cla018360 was highly expressed in young leaves.Phylogenetic analysis showed that Cla018360 had a close genetic relationship with AtHB51,which had been reported to regulate the formation of leaf shape in Arabidopsis.Furthermore,transcriptome analysis showed that a total of 333 differentially expressed genes were identified between WT2 and WT20,of which 115 and 218 genes were upregulated and downregulated in no-lobed leaved watermelon WT20.This study not only provides a good entry point for studying leaf development but also provides foundational insights into breeding for special plant architecture in watermelon.
文摘Partial epilepsies, originating in a specific brain region, affect about 60% of adults with epilepsy. Temporal lobe epilepsy (TLE) is the most prevalent subtype within this category, often necessitating surgical intervention due to its refractoriness to antiepileptic drugs (AEDs). Hippocampal sclerosis, a common underlying pathology, often exacerbates the severity by introducing cognitive and emotional challenges. This review delves deeper into the cognitive profile of TLE, along with the risk factors for cognitive disorders, depression, and anxiety in this population.
文摘The performance of a newly designed tri-lobe industrial lobe pump of high capacity is simulated by using commercial CFD solver Ansys Fluent. A combination of user-defined-functions and meshing strategies is employed to capture the rotation of the lobes. The numerical model is validated by comparing the simulated results with the literature values. The processes of suction, displacement, compression and exhaust are accurately captured in the transient simulation. The fluid pressure value remains in the range of inlet pressure value till the processes of suction and displacement are over. The instantaneous process of compression is accurately captured in the simulation. The movement of a particular working chamber is traced along the gradual degree of lobe’s rotation. At five different degrees of lobe’s rotation, pressure contour plots are reported which clearly shows the pressure values inside the working chamber. Each pressure value inside the working chamber conforms to the particular process in which the working chamber is operating. Finally, the power requirement at the shaft of rotation is estimated from the simulated values. The estimated value of power requirement is 3.61 BHP FHP whereas the same calculated theoretically is 3 BHP FHP. The discrepancy is attributed to the assumption of symmetry of blower along the thickness.
基金the Natural Science Foundation of the Fujian Province,No.2021J01876.
文摘BACKGROUND Gelastic seizure(GS)is a rare type of epilepsy that most commonly appears in patients with hypothalamic hamartoma.It is rarely associated with other types of brain damage.This particular type of epilepsy is relatively rare and has few links to other brain lesions.Temporal lobe malacia is mostly caused by cerebral infarction or cerebral hemorrhage,which can lead to seizures.We report a case of GS in a woman with temporal lobe malacia which was reported for the first time in the literature.CASE SUMMARY A 73-year-old female,diagnosed case of GS,presented with repetitive stereotyped laughter a month prior to presentation,happening multiple times daily and with each time lasting for 5-15s.Electroencephalogram displayed a focal seizure seen in the right temporal region.Magnetic resonance imaging head with contrast showed a right temporal lobe malacia.The patient was started on levetiracetam daily.The patient indicated that they had fully recovered and were not experiencing any recurrent or stereotyped laughter during their daily routines.These results remained consistent even after a one-year follow-up period.CONCLUSION GS can be caused by temporal lobe malacia,which is an uncommon but potentially grave condition.The outcome of this present case exhibited the importance of the temporal lobe in the genesis of GS.
基金supported by the National Natural Science Foundation of China,Nos.62027812(to HS),81771470(to HS),and 82101608(to YL)Tianjin Postgraduate Research and Innovation Project,No.2020YJSS122(to XD)。
文摘Temporal lobe epilepsy is a multifactorial neurological dysfunction syndrome that is refractory,resistant to antiepileptic drugs,and has a high recurrence rate.The pathogenesis of temporal lobe epilepsy is complex and is not fully understood.Intracellular calcium dynamics have been implicated in temporal lobe epilepsy.However,the effect of fluctuating calcium activity in CA1 pyramidal neurons on temporal lobe epilepsy is unknown,and no longitudinal studies have investigated calcium activity in pyramidal neurons in the hippocampal CA1 and primary motor cortex M1 of freely moving mice.In this study,we used a multichannel fiber photometry system to continuously record calcium signals in CA1 and M1 during the temporal lobe epilepsy process.We found that calcium signals varied according to the grade of temporal lobe epilepsy episodes.In particular,cortical spreading depression,which has recently been frequently used to represent the continuously and substantially increased calcium signals,was found to correspond to complex and severe behavioral characteristics of temporal lobe epilepsy ranging from gradeⅡto gradeⅤ.However,vigorous calcium oscillations and highly synchronized calcium signals in CA1 and M1 were strongly related to convulsive motor seizures.Chemogenetic inhibition of pyramidal neurons in CA1 significantly attenuated the amplitudes of the calcium signals corresponding to gradeⅠepisodes.In addition,the latency of cortical spreading depression was prolonged,and the above-mentioned abnormal calcium signals in CA1 and M1 were also significantly reduced.Intriguingly,it was possible to rescue the altered intracellular calcium dynamics.Via simultaneous analysis of calcium signals and epileptic behaviors,we found that the progression of temporal lobe epilepsy was alleviated when specific calcium signals were reduced,and that the end-point behaviors of temporal lobe epilepsy were improved.Our results indicate that the calcium dynamic between CA1 and M1 may reflect specific epileptic behaviors corresponding to different grades.Furthermore,the selective regulation of abnormal calcium signals in CA1 pyramidal neurons appears to effectively alleviate temporal lobe epilepsy,thereby providing a potential molecular mechanism for a new temporal lobe epilepsy diagnosis and treatment strategy.
基金Key Clinical Specialty Discipline Construction Program of Fujian,Fujian Health Medicine and Politics,No.[2022]884.
文摘BACKGROUND In laparoscopic proximal gastrectomy(LPG),the prolapse of the hepatic left lateral lobe near the lesser curvature and esophageal hiatus can obstruct the field of vision and operation.Therefore,it is necessary to retract or obstruct the hepatic left lateral lobe to ensure a clear field of vision.AIM To investigate the safety and clinical efficacy of the modified hepatic left lateral lobe inversion technique for LPG.METHODS A retrospective analysis was conducted on the clinical data of 13 consecutive patients with early-stage upper gastric adenocarcinoma or adenocarcinoma of the esophagogastric junction treated with LPG from January to December 2023 at the Department of Gastrointestinal Surgery,Second Affiliated Hospital of Fujian Medical University.The modified hepatic left lateral lobe inversion technique was used to expose the surgical field in all patients,and short-term outcomes were observed.RESULTS In all 13 patients,the modified hepatic left lateral lobe inversion technique was successful during surgery without the need for re-retraction or alteration of the liver traction method.There were no instances of esophageal hiatus occlusion,eliminating the need for forceps to assist in exposure.There was no occurrence of intraoperative hepatic hemorrhage,hepatic vein injury,or hepatic congestion.No postoperative digestive complications of Clavien-Dindo grade≥II occurred wi-thin 30 days after surgery,except for a single case of pulmonary infection.Some patients experienced increases in alanine aminotransferase and aspartate aminotransferase levels on the first day after surgery,which significantly decreased by the third day and returned to normal by the seventh day after surgery.CONCLUSION The modified hepatic left lateral lobe inversion technique has demonstrated satisfactory results,offering ad-vantages in terms of facilitating surgical procedures,reducing surgical trauma,and protecting the liver.
基金Supported by the Civil Aviation Research Foundation of Nanjing University of Aeronautics and Astronautics~~
文摘The flow field and aerodynamic performances for the scarfed lobed forced mixer are studied based on a computational fluid dynamics(CFD) technique. A series of computations are conducted to obtain the effects of the bypass ratio and the scarf angle on the mixing performance for the scarfed lobed mixer. Results show that the scarfed lobed mixer is reduced in the system weight. Meanwhile, aerodynamic performances are slightly improved compared with the normal lobed mixer. Two reasons for causing the mixing enhancement between the core and the bypass flow are as follows: (1) The stream-wise vortices shed from the training edge of the half/full scarfed lobed mixer earlier is enhanced by about 25%. (2) The mixing augmentation is also associated with the increase of the interface length caused by scarfing. The thermal mixing efficiency is enhanced with the increase of the bypass ratio and the scarfing angle. The scarfed lobed mixer design has no negative effects on the pressure loss. The total pressure recovery coefficient reaches above 0. 935 in various bypass ratios and scarfed angles. As the bypass ratio increases, the total pressure recovery coefficient also increases for the scarfed lobed mixer.
基金Supported by National Natural Science Foundation of China(31100987)Project of Shandong University of Technology(4040-306018)Young Teacher Development Plan of Shandong University of Technology~~
文摘[Objective] We aimed to investigate the preventive and therapeutical effect of compound of traditional Chinese drugs (Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root) on acute alcohol intoxication in mice. [Method] Acute alcohol intoxication was induced by administering alcohol to mice. Three different doses (low, middle and high) of compound of traditional Chinese drugs were administered to mice before and after administering alcohol respectively to investigate the preventive and therapeutical effect of drugs on acute alcohol intox-ication through doing statistical analysis about drunk mice and their sleeping time. The concentration of malondialdehyde (MDA), reduced glutathione (GSH) and triglyc-erides (TG) in liver was also determined to investigate the protective effect of drugs on liver. [Result] The efficacy of compound of traditional Chinese drugs on acute al-cohol intoxication was dose-dependent. High-dose administration decreased the number of drunk mice significantly compared with control group; middle- and high-dose administration reduced the sleeping time of drunk mice and the concentration of MDA and TG in liver tissue; three doses al increased the concentration of GSH. [Conclusion] The compound of Japanese raisintree fruit, lobed kudzuvine flower bud and lightyel ow sophora root had preventive and therapeutical effect on hangover, and it also had certain preventive and therapeutical effect on liver damage caused by alcohol.
文摘Three-dimensional numerical computation of the flow fields and pumping performances for the lobed mixer-ejector are conducted using full Navier-Stokes equations. In the computation, the inlet of the primary flow uses the mass flowrate boundary condition. The inlet of the second flow and the outlet of the mixing flow use the pressure boundary condition. Compared with the relative experimental resuits, it is shown that the present calculation is reasonable. And a series of numerical studies is performed to obtain the effects of area ratio and length-to-diameter ratio of mixing duct on pumping coefficient and thermal mixing efficiency of a lobed mixer-ejector.
文摘Temporal lobe epilepsy is the most common form of focal epilepsy in adults,accounting for one third of all diagnosed epileptic patients,with seizures originating from or involving mesial temporal structures such as the hippocampus,and many of these patients being refractory to treatment with anti-epileptic drugs.Temporal lobe epilepsy is the most common childhood neurological disorder and,compared with adults,the symptoms are greatly affected by age and brain development.Diagnosis of temporal lobe epilepsy relies on clinical examination,patient history,electroencephalographic recordings,and brain imaging.Misdiagnosis or delay in diagnosis is common.A molecular biomarker that could distinguish epilepsy from healthy subjects and other neurological conditions would allow for an earlier and more accurate diagnosis and appropriate treatment to be initiated.Among possible biomarkers of pathological changes as well as potential therapeutic targets in the epileptic brain are micro RNAs.Most of the recent studies had performed micro RNA profiling in body fluids such as blood plasma and blood serum and brain tissues such as temporal cortex tissue and hippocampal tissue.A large number of micro RNAs were dysregulated when compared to healthy controls and with some overlap between individual studies that could serve as potential biomarkers.For example,in adults with temporal lobe epilepsy,possible biomarkers are miR-199a-3p in blood plasma and miR-142-5p in blood plasma and blood serum.In adults with mesial temporal lobe epilepsy,possible biomarkers are miR-153 in blood plasma and miR-145-3p in blood serum.However,in many of the studies involving patients who receive one or several anti-epileptic drugs,the influence of these on micro RNA expression in body fluids and brain tissues is largely unknown.Further studies are warranted with children with temporal lobe epilepsy and consideration should be given to utilizing mouse or rat and non-human primate models of temporal lobe epilepsy.The animal models could be used to confirm micro RNA findings in human patients and to test the effects of targeting specific micro RNAs on disease progression and behavior.
文摘An experimental study was conducted to investigate the evolutions of unsteady vortex structures downstream a lobed mixer/nozzle.A novel dual-plane stereoscopic PIV system was used to measure all 3-components of vorticity distributions to revealed both the large-scale streamwise vortices produced by the lobed mixer/nozzle and the Kelvin-Helmholtz vortex structures generated due to the Kelvin-Helmholtz instabilities simultaneously and quantitatively for the first time.The instantaneous and the ensemble-averaged vorticity distributions displayed quite different aspects about the evolutions of the unsteady vortex structures.While the ensemble-averaged vorticity distributions indicated the overall effect of the special geometry of the lobed nozzle/mixer on the enhanced mixing process,the instantaneous vorticity distributions elucidated many details about how the enhanced mixing process was conducted.In addition to quantitatively confirming conjectures of previous studies,further insight about the formation,evolution and interaction characteristics of the unsteady vortex structures downstream of the lobed mixer/nozzle were also uncovered quantitatively in the present study.
基金supported by grants from Natural Science Foundation of Zhejiang Province (LY20H180011)National Natural Science Foundation of China (81970653)Medical and Health Science and Technology project of Zhejiang (2016KYA061)
文摘Background: Improving the mechanical properties and angiogenesis of acellular scaffolds before transplantation is an important challenge facing the development of acellular liver grafts. The present study aimed to evaluate the cytotoxicity and angiogenesis of polyethylene glycol(PEG) crosslinked decellularized single liver lobe scaffolds(DLSs), and establish its suitability as a graft for long-term liver tissue engineering. Methods: Using mercaptoacrylate produced by the Michael addition reaction, DLSs were first modified using N-succinimidyl S-acetylthioacetate(SATA), followed by cross-linking with PEG as well as vascular endothelial growth factor(VEGF). The optimal concentration of agents and time of the individual steps were identified in this procedure through biomechanical testing and morphological analysis. Subsequently, human umbilical vein endothelial cells(HUVECs) were seeded on the PEG crosslinked scaffolds to detect the proliferation and viability of cells. The scaffolds were then transplanted into the subcutaneous tissue of Sprague-Dawley rats to evaluate angiogenesis. In addition, the average number of blood vessels was evaluated in the grafts with or without PEG at days 7, 14, and 21 after implantation. Results: The PEG crosslinked DLS maintained their three-dimensional structure and were more translucent after decellularization than native DLS, which presented a denser and more porous network structure. The results for Young’s modulus proved that the mechanical properties of 0.5 PEG crosslinked DLS were the best and close to that of native livers. The PEG-VEGF-DLS could better promote cell proliferation and differentiation of HUVECs compared with the groups without PEG cross-linking. Importantly, the average density of blood vessels was higher in the PEG-VEGF-DLS than that in other groups at days 7, 14, and 21 after implantation in vivo. Conclusions: The PEG crosslinked DLS with VEGF could improve the biomechanical properties of native DLS, and most importantly, their lack of cytotoxicity provides a new route to promote the proliferation of cells in vitro and angiogenesis in vivo in liver tissue engineering.
文摘In this research work,we present the evolution of Coulomb failure stress(CFS)in the Sulaiman Lobe and its implications for seismic hazard assessment.The Chaman transform fault,~1,000 km long,is the major active fault that marks the western boundary between Pakistan and Afghanistan on the Indian Plate.To date,few studies have been conducted to unveil the interactions among earthquakes and the implications of these interactions for seismic hazard assessment in the region.We thoroughly investigated the published and online catalog to construct a sequence of major earthquakes that occurred in this region during the past.The final earthquake sequence was composed of 15 earthquakes of M_(w)≥6.0,beginning with the 1888 earthquake.We used the stress-triggering theory to numerically simulate the evolution of CFS caused by these earthquakes.The numerical results revealed that 8 out of 15earthquakes were triggered by the preceding earthquakes.The earthquakes in 1908,1910,1935,1966,and 1997 were rather independent earthquakes in this sequence.Although the epicenters of the 1975a and 1975b earthquakes were in the stress shadow zone,the partial rupture segments of both these earthquakes were in high-CFS regions.The CFS induced by the 1935 earthquake was notable,as it later triggered the 2008 doublet.Moreover,our results revealed that the northern segment of the Chaman Fault,the southern segment of the Ghazaband Fault,and the northwestern segment of the Urghargai Fault demonstrated a high change in CFS that could trigger seismicity in these regions.The necessary arrangements must therefore be made to mitigate any possible seismic hazards in the region.
基金supported by the Lia⁃oning Provincial Natural Science Joint Fund Project(No.120316013).
文摘In order to investigate the effect of the radial gradation of the lobed nozzles on the flow field organization,a cold water model experimental platform for a combustion chamber with radial⁃staged 13-point lobed nozzles is built.Compared with a series of combustion OH*luminescence experiments tested by the University of Cincinnati,the four corresponding working conditions of no load,partial load,cruise and take off are selected.The vortex structure,vorticity value,multi-combustion materiel field and combustion characteristics of the flow field in the radial staged combustion chamber of the lobed nozzles under the equivalence ratio,the fuel injection method,the fuel injection ratio and other factors are numerically studied.The results show that under different influencing factors,the varation trend of the hydroxyl flame field of the lobe combustion chamber is basically the same as that of the hydroxyl light emission experiment of the swirl combustion chamber,but the flame field shape is quite different.The local equivalent ratio has a greater influence on the relevant combustion performance of the combustion chamber.Under the conditions of lower equivalence ratio,three-stage air and fuel injection mode,and gradually transferring the fuel flow of the pilot circuit to the external circuit,the temperature field and flame field of the combustion chamber are more evenly distributed,the outlet temperature field quality is better,the combustion efficiency is higher,and the NO_(X) emission is relatively low.These are basically consistent with the cold test results.The cold experimental results illustrate the importance of the influence of the flow field organization on the combustion organization and verify the reliability of the calculation results.
基金National Natural Science Foundation of China,Nos.82003 729 (to Ying W),82022071 (to YiW)Natural Science Foundation of Shandong Province of China,No.ZR2020QH357 (to Ying W)Public Welfare Technology Research Program of Zhejiang Province,No.LGF20H09001 1 (to JF)。
文摘Cognitive impairment is the most common complication in patients with temporal lobe epilepsy with hippocampal scle rosis.There is no effective treatment for cognitive impairment.Medial septum cholinergic neurons have been reported to be a potential target for controlling epileptic seizures in tempo ral lobe epile psy.However,their role in the cognitive impairment of temporal lobe epilepsy remains unclear.In this study,we found that patients with temporal lobe epile psy with hippocampal sclerosis had a low memory quotient and severe impairment in verbal memory,but had no impairment in nonverbal memory.The cognitive impairment was slightly correlated with reduced medial septum volume and medial septum-hippocampus tra cts measured by diffusion tensor imaging.In a mouse model of chronic temporal lobe epilepsy induced by kainic acid,the number of medial septum choline rgic neurons was reduced and acetylcholine release was reduced in the hippocampus.Furthermore,selective apoptosis of medial septum cholinergic neurons mimicked the cognitive deficits in epileptic mice,and activation of medial septum cholinergic neurons enhanced hippocampal acetylcholine release and restored cognitive function in both kainic acid-and kindling-induced epile psy models.These res ults suggest that activation of medial septum cholinergic neurons reduces cognitive deficits in temporal lobe epilepsy by increasing acetylcholine release via projections to the hippocampus.