Establishment of BALB/c Mice Model for Food Allergy to Chinese Lobsters

[Objective] This study aimed to establish Balb/c mice model for food allergy caused by Chinese lobsters through using intraperitoneal injection for sensitization and explore methods for in vitro identification and evaluation of food allergy caused by Chinese lobsters. [Method] The 40 male Bal/c mice were divided into ovalbumin（OVA） positive control group, Coca＇s solution negative control group, blank control group and model group. Balb/c mice model was established by intraperitoneally injection of immunized Balb/c mice with OVA or Chinese lobster crude protein with aluminum hydroxide adjuvant. IgE and histamine levels in serum after the second challenge were determined by ELISA method, and the specific IgE antibody titer was determined by passive cutaneous anaphylaxis test（PCA）; additionally, spleen index and histological changes in the small intestine, as well as food allergy symptoms after challenge were also calculated or observed. [Results] After the last challenge, IgE content was（236.75 ±73.39） μg/L in the Chinese lobster crude protein group, revealing no difference with that in the OVA group, but significantly higher than that in either the Coca＇s solution group or the blank control group（P 0.01）;histamine content in serum in the Chinese lobster crude protein group was（406.55±232.79）, significantly higher than that in the blank control group（P0.01）. In the passive cutaneous anaphylaxis test, IgE antibody titer reached 1/16 after the last challenge in the Chinese lobster crude protein group. Spleen index in both Chinese lobster crude protein group and OVA group was significantly greater than that in either the Coca＇s solution group or the blank control group（P0.01）. What＇s more, infiltration of inflammatory cells like lymphocytes and eosnophils at the lamina propria of intestinal mucosa was also observed both Chinese lobster crude protein group and OVA group. [Conclusion] This study established Balb/c mice model for food allergy caused by Chinese lobsters; serum IgE and ELISA assay and specific IgE antibody titer in PCA test can be used for the in vitro identification and evaluation of food allergies caused by Chinese lobsters.

LOBSTER与MOG结合的目标检测方法

借助深度相机数据一定程度上解决了目标检测中的颜色伪装问题,但又导致深度伪装问题。针对这些问题,提出一种利用两种背景差分法分别作用于颜色和深度数据的目标检测方法。在场景颜色图像中采用基于改进的局部二值相似性模式(LOBSTER)的背景差分法检测运动目标;在深度图中,对孔洞(无深度值)像素和有深度值的像素分别进行背景建模,得到一个混合的背景模型,然后用混合高斯模型背景差分法得到深度图的二值图像,再用差分后的Canny边缘检测图来补充目标的轮廓;将两种二值图像与Canny边缘检测的差分图用逻辑运算进行结合,形成运动目标。在后期处理中,加入了孔洞填充、形态学滤波及腐蚀膨胀操作,进一步提高目标的检测精度。实验结果表明,上述方法在测试数据集上得到的目标检测精度较高。

Lobster Fishery Connectivity and Management In Indonesia Waters

The distribution of lobsters in Indonesian waters is very wide,even lobster species in Indonesia are also scattered in the tropical waters of the western Pacific Ocean,the Indian Ocean,Africa to Japanese waters.Indonesian waters are divided into 11(eleven)Fishery Management Zone(FMZ).Lobsters in Indonesia may come from various water areas,both national and regional water zones,and they’re called the sink population.Its spread is influenced by the movement of the current.Lobster seed is nurtured by nature through ocean currents from Australia,East Indonesia,Japan,then back to Australia.Lobsters have a complex life cycle,where adult lobsters inhabit coral reefs as a place to lay eggs,then hatch into planktonic larvae,and grow up in open seas and carry out diurnal and ontogenetic vertical migrations before returning to nurseries in shallow coastal areas and reefs,coral,as well as habitat by the type of species.Literature research had used at least two methodologies to estimate the distribution and connection sensitivity matrices of marine organism larvae.The two most common approaches are using genetic markers and numerical biophysical modeling.Thus,this research uses molecular genetic techniques to explain the ge­netic structure of lobster populations using a biophysical model approach that can explain the genetic structure of lobsters,as well as the distribution based on regional oceanographic synthesis data and lobster biology known in Indonesian waters.This model has four components,namely:1)a ben­thic module based on a Geographical Information System(GIS)which is a lobster habitat in the spawning and recruitment process,2)a physical oceanography module containing daily velocity in the form of a three-di­mensional hydrodynamic model,3)a larva biology module that describes larval life history characteristics,and 4)a Lagrangian Stochastic module that tracks the individual trajectories of larvae.

Sustainability level of the pearl lobster(Panulirus ornatus)aquaculture business using the systems of floating-net cage and fixed-net cage:A case study in South Sulawesi Province,Indonesia

Marine aquaculture is currently the fastest-growing seafood industry globally,offering a potential solution to overfishing problems,while enhancing food security and income generation.South Sulawesi,a province in Indonesia,is rich in aquaculture resources,one of which is lobster.The two commonly used aquaculture systems are the floating-net cage(KJA)and fixed-net cage(KJD).The growing market demand and rising lobster prices pose a threat to resource sustainability due to overexploitation.This is issue have to be addressed to achieve sustainable fishery development.Sustainability is the primary focus on global fishery development.According to the United Nations,sustainable development encompasses three dimensions:the economy,social and ecology.Therefore,sustainable fishery,particularly aquaculture,relies on obtaining significant benefits while minimizing environmental damage.Hence,this study aimed to analyze the sustainability level of the pearl lobster(Panulirus ornatus)aquaculture in South Sulawesi,based on the provided background information.The study included a sample of 62 farmers.The sample selection involved the use of the stratified sampling method,with 51 farmers from(KJA)and 11 farmers from(KJD).The sustainability analysis,conducted through assessment of based farmers’perceptions,served as the analytical method.The findings revealed that pearl lobster(Panulirus ornatus)aquaculture has a medium level of sustainability.Employing Integrated Multi Tropic Aquaculture(IMTA)and Regional Integrated Multi Tropic Aquaculture(RIMTA)techniques is a recommended sustainability level.

动态背景下自适应LOBSTER算法的前景检测

目的前景检测是视频监控领域的研究重点之一。LOBSTER(local binary similarity segmenter)算法把Vi Be(visual background extractor)算法和LBSP(local binary similarity patterns)特征结合起来,在一般场景下取的了优良的检测性能,但是LOBSTER算法在动态背景下适应性差、检测噪声多。针对上述问题,提出一种改进的LOBSTER算法。方法在模型初始化阶段,计算各像素的LBSP特征值,并分别把像素的灰度值和LBSP特征值添加到各像素的颜色背景模型与LBSP背景模型中,增强了背景模型的描述能力;在像素分类阶段,根据背景复杂度自适应调整每个像素在颜色背景模型和LBSP背景模型中的分类阈值,降低了前景中的噪声;在模型更新阶段,根据背景复杂度自适应调整每个像素背景模型的更新策略,提高背景模型对动态背景的适应能力。结果本文算法与Vi Be算法和LOBSTER算法进行了对比实验,本文算法的前景图像比Vi Be算法和LOBSTER算法的噪声点大幅较低,本文算法的PCC指标在不同视频库中比Vi Be算法提高0.736%7.56%,比LOBSTER算法提高0.77%12.47%,FPR指标不到Vi Be算法和LOBSTER算法的1%。结论实验仿真结果表明,在动态背景的场景下,本文算法比Vi Be算法和LOBSTER算法检测到的噪声少,具有较高的准确率和鲁棒性。

Structure and mechanical properties of pincers for freshwater lobster

The mineral phases and structure of pincer for the freshwater lobster were analyzed and observed by using X-ray diffraction and field-emission scanning electronic microscope with energy dispersive spectroscopy, and the mechanical properties of pincer were determined by using nano-indenter and micro-tribometer. The results showed that the pincers contained Ca, Mg, C, O, Na, etc. elements and there were many amorphous mineral phases in the lobster pincers. There were many concave hollows and convex domes with seta on the surface of pincer, and some spines were observed on the surface of seta, which exhibited non-smooth surface. The exoskeleton of pincer consisted of epicuticle, exocuticle and endocuticle, and the microstructure of exocuticle and endocuticle displayed the twisted plywood structure with chitin protein fibers. The mean hardness and elastic modulus on the surface of fresh pincers were 0.27 GPa and 5.28 GPa, respectively. With the increase in distance from the surface, the hardness and elastic modulus all decreased gradually. This indicated that the transition of hardness and elastic modulus occurred from exocuticle to endocuticle. When the heat treatment temperature was lower than 200°C, the values of hardness and elastic modulus fluctuated slightly, while they increased at the temperature beyond 200°C. As the pincers slid against Si3N4 balls in water and air, the friction coefficients in water were higher than those in air. The research could provide a bio-inspired basis for the structural design of composite materials and anti-friction surface.