According to the large-scale sequencing of cDNA library from silkworm pupae, the cDNA of a novel gene with blank research background was identified and temporarily named Bm-X. The length of this cDNA is 778 bp. We obt...According to the large-scale sequencing of cDNA library from silkworm pupae, the cDNA of a novel gene with blank research background was identified and temporarily named Bm-X. The length of this cDNA is 778 bp. We obtained its ORF for further study by bioinformatics analysis. It is 444 bp and encodes 147 amino acid residues, with a predicted molecular weight (MW) of 16.4 kD and an isoelectric point (pI) of 3.69. In this study, we successfully constructed a recombinant plasmid pET-28a(+)-Bm-X and expressed it in Escherichia coli. We used the fusion protein rBm-X which purified by Niaffinity chromatography to produce polyclonal antibodies against Bm-X for Western blot analysis. The analysis revealed that Bm-X was expressed in the larval midgut, the epidermis and the silk gland. In addition, the subcellular localization analysis of silkworm ovary epithelial cells (BmN cells) showed that Bm-X protein was located both in cytoplasm and nucleus, and the signal was stronger in cytoplasm than in nucleus. Our findings indicate that Bm-X gene is a novel species-specificity gene and its expression product can be detected in tissues of the fifth silkworm instar larvae and BmN cells.展开更多
Macroscopic productions of “non-locality” or “excess correlations” of dynamic changes within media between two spaces could be utilized as alternative communication systems. Previous experiments have shown that in...Macroscopic productions of “non-locality” or “excess correlations” of dynamic changes within media between two spaces could be utilized as alternative communication systems. Previous experiments have shown that injections of a weak acid within one of two volumes of spring water sharing the same patterned circular magnetic fields with changing angular accelerations separated by non-traditional (5 m) distances were associated with opposite (basic) shifts in pH within the non-injected, non-local volume. In the present experiments, employing a different technology, pairs of beakers separated by 1 m containing either 25 cc, 50 cc, or 100 cc of spring water were placed within toroids generating weak (30, 300 nT) changing acceleration magnetic fields with 1 ms, 2 ms, or 3 ms point durations or a field whose point durations changed. When a proton source (weak acid) was injected into one beaker (local) pH shifts in the other (non-local) beaker exhibit increased acidity for the 3 ms point duration but increased alkalinity for the 1 ms duration. Neither intermittent point durations nor variable point durations for the same volumes of water placed between the two magnetic field-coupled beakers exhibited significant changes from baseline. Contingent upon the point duration of the applied field, the pH shift was consistent with a fixed quantity of decreased free protons (increased pH) or increased protons (decreased pH) in the non-local beakers. The opposite directions of the pH shifts at 1 ms and 3 ms that correspond to quantitative cosmological solutions for electrons and protons suggest these results may reflect a fundamental physical process.展开更多
文摘According to the large-scale sequencing of cDNA library from silkworm pupae, the cDNA of a novel gene with blank research background was identified and temporarily named Bm-X. The length of this cDNA is 778 bp. We obtained its ORF for further study by bioinformatics analysis. It is 444 bp and encodes 147 amino acid residues, with a predicted molecular weight (MW) of 16.4 kD and an isoelectric point (pI) of 3.69. In this study, we successfully constructed a recombinant plasmid pET-28a(+)-Bm-X and expressed it in Escherichia coli. We used the fusion protein rBm-X which purified by Niaffinity chromatography to produce polyclonal antibodies against Bm-X for Western blot analysis. The analysis revealed that Bm-X was expressed in the larval midgut, the epidermis and the silk gland. In addition, the subcellular localization analysis of silkworm ovary epithelial cells (BmN cells) showed that Bm-X protein was located both in cytoplasm and nucleus, and the signal was stronger in cytoplasm than in nucleus. Our findings indicate that Bm-X gene is a novel species-specificity gene and its expression product can be detected in tissues of the fifth silkworm instar larvae and BmN cells.
文摘Macroscopic productions of “non-locality” or “excess correlations” of dynamic changes within media between two spaces could be utilized as alternative communication systems. Previous experiments have shown that injections of a weak acid within one of two volumes of spring water sharing the same patterned circular magnetic fields with changing angular accelerations separated by non-traditional (5 m) distances were associated with opposite (basic) shifts in pH within the non-injected, non-local volume. In the present experiments, employing a different technology, pairs of beakers separated by 1 m containing either 25 cc, 50 cc, or 100 cc of spring water were placed within toroids generating weak (30, 300 nT) changing acceleration magnetic fields with 1 ms, 2 ms, or 3 ms point durations or a field whose point durations changed. When a proton source (weak acid) was injected into one beaker (local) pH shifts in the other (non-local) beaker exhibit increased acidity for the 3 ms point duration but increased alkalinity for the 1 ms duration. Neither intermittent point durations nor variable point durations for the same volumes of water placed between the two magnetic field-coupled beakers exhibited significant changes from baseline. Contingent upon the point duration of the applied field, the pH shift was consistent with a fixed quantity of decreased free protons (increased pH) or increased protons (decreased pH) in the non-local beakers. The opposite directions of the pH shifts at 1 ms and 3 ms that correspond to quantitative cosmological solutions for electrons and protons suggest these results may reflect a fundamental physical process.
