The homotopy formulas of (r,s) differential forms and the solution of equation of type (r,s) on local q-convex domains in Stein manifolds are obtained.The homotopy formulas on local q-convex domains have important app...The homotopy formulas of (r,s) differential forms and the solution of equation of type (r,s) on local q-convex domains in Stein manifolds are obtained.The homotopy formulas on local q-convex domains have important applications in uniform estimates of equation and holomorphic extension of CR-manifolds.展开更多
Immune evolutionary algorithms with domain knowledge were presented to solve the problem of simultaneous localization and mapping for a mobile robot in unknown environments. Two operators with domain knowledge were de...Immune evolutionary algorithms with domain knowledge were presented to solve the problem of simultaneous localization and mapping for a mobile robot in unknown environments. Two operators with domain knowledge were designed in algorithms, where the feature of parallel line segments without the problem of data association was used to construct a vaccination operator, and the characters of convex vertices in polygonal obstacle were extended to develop a pulling operator of key point grid. The experimental results of a real mobile robot show that the computational expensiveness of algorithms designed is less than other evolutionary algorithms for simultaneous localization and mapping and the maps obtained are very accurate. Because immune evolutionary algorithms with domain knowledge have some advantages, the convergence rate of designed algorithms is about 44% higher than those of other algorithms.展开更多
In this paper, we give the four equivalent characterizations for the weighted local hardy spaces on Lipschitz domains. Also, we give their application for the harmonic function defined in bounded Lipschitz domains.
The current study investigated correlations between the expression of leucine-rich repeats and immunoglobulin-like domain 1 (LRIG1) and antioxidant enzymes and related proteins, including manganese superoxide dismut...The current study investigated correlations between the expression of leucine-rich repeats and immunoglobulin-like domain 1 (LRIG1) and antioxidant enzymes and related proteins, including manganese superoxide dismutase, glutamate cysteine ligase catalytic or regulatory subunit, thioredoxin and thioredoxin reductase, in both human ependymoma and oligodendroglioma. Results revealed that the cytoplasmic expression of LRIG1 was associated with expression of glutamate cysteine ligase catalytic subunit in the human ependymoma, while the nuclear expression of LRIG1 was associated with expression of thioredoxin reductase. In human oligodendroglioma, the cytoplasmic expression of LRIG1 was associated with expression of the glutamate cysteine ligase catalytic subunit. Both the nuclear and perinuclear expressions of LRIG1 were associated with expression of glutamate cysteine ligase regulatory subunit. These results indicated that several antioxidant enzymes and related proteins contributed to LRIG1 expression, and that these may participate in the antioxidation of the cells.展开更多
It is proved that the global existence for the nonhomogeneous quasilinear wave equation with a localized weakly nonlinear dissipation in exterior domains.
Let Ω be a bounded convex domain in Rn(n≥3) and G(x,y) be the Green function of the Laplace operator -△on Ω Let hPT(Ω) = {f∈D'(Ω) : (?)F ∈ hP(Rn), s.t. F|Ω = f}, by the atom characterization of Local Hard...Let Ω be a bounded convex domain in Rn(n≥3) and G(x,y) be the Green function of the Laplace operator -△on Ω Let hPT(Ω) = {f∈D'(Ω) : (?)F ∈ hP(Rn), s.t. F|Ω = f}, by the atom characterization of Local Hardy spaces in a bounded Lipschitz domain, the bound of f Ω(?)2(Gf) for every f ∈hPr(Ω) is obtained, where n/(n + 1) <p≤1.展开更多
Recent experiments revealed many new phenomena of the macroscopic domain patterns in the stress-induced phase transformation of a superelastic polycrystalline NiTi tube during tensile loading. The new phenomena includ...Recent experiments revealed many new phenomena of the macroscopic domain patterns in the stress-induced phase transformation of a superelastic polycrystalline NiTi tube during tensile loading. The new phenomena include deformation instability with the formation of a helical domain, domain topology transition from helix to cylinder, domain-front branching and loading-path dependence of domain patterns. In this paper, we model the polycrystal as an elastic continuum with nonconvex strain energy and adopt the non-local strain gradient energy to account for the energy of the diffusive domain front. We simulate the equilibrium domain patterns and their evolution in the tubes under tensile loading by a non-local Finite Element Method (FEM). It is revealed that the observed loading-path dependence and topology transition of do- main patterns are due to the thermodynamic metastability of the tube system. The computation also shows that the tube-wall thickness has a significant effect on the domain patterns: with fixed material properties and interfacial energy density, a large tube-wall thickness leads to a long and slim helical domain and a severe branching of the cylindrical-domain front.展开更多
An efficient conformal locally one-dimensional finite-difference time-domain(LOD-CFDTD) method is presented for solving two-dimensional(2D) electromagnetic(EM) scattering problems. The formulation for the 2D tra...An efficient conformal locally one-dimensional finite-difference time-domain(LOD-CFDTD) method is presented for solving two-dimensional(2D) electromagnetic(EM) scattering problems. The formulation for the 2D transverse-electric(TE) case is presented and its stability property and numerical dispersion relationship are theoretically investigated. It is shown that the introduction of irregular grids will not damage the numerical stability. Instead of the staircasing approximation, the conformal scheme is only employed to model the curve boundaries, whereas the standard Yee grids are used for the remaining regions. As the irregular grids account for a very small percentage of the total space grids, the conformal scheme has little effect on the numerical dispersion. Moreover, the proposed method, which requires fewer arithmetic operations than the alternating-direction-implicit(ADI) CFDTD method, leads to a further reduction of the CPU time. With the total-field/scattered-field(TF/SF) boundary and the perfectly matched layer(PML), the radar cross section(RCS) of two2 D structures is calculated. The numerical examples verify the accuracy and efficiency of the proposed method.展开更多
引入局部条件并半格 (简记为 L cusl)及其理想完备化等概念 .证明了 :任一代数 L domain的紧元集是 L cusl;任一代数 L domain是其紧元集赋予 Alexandrov拓扑时的 Sober化 ;任一 L cusl的理想完备化是代数 L domain,从而得到了代数 L do...引入局部条件并半格 (简记为 L cusl)及其理想完备化等概念 .证明了 :任一代数 L domain的紧元集是 L cusl;任一代数 L domain是其紧元集赋予 Alexandrov拓扑时的 Sober化 ;任一 L cusl的理想完备化是代数 L domain,从而得到了代数 L domain的表示定理 .还证明了 Scott连续映射为态射的代数 L domain范畴为 L cusl与单调映射作成的范畴的反射子范畴 .展开更多
The study aims to detect the subcellular localization of ERF (ethylene-responsive element binding factor) transcription factor W17 protein, the interaction between W 17 and cis-acting regulatory elements GCC-box and...The study aims to detect the subcellular localization of ERF (ethylene-responsive element binding factor) transcription factor W17 protein, the interaction between W 17 and cis-acting regulatory elements GCC-box and DRE in vitro, the binding and transactivating ability in vivo, and the role of W17 in higher plant stress-signal pathway. Recombinant plasmid W17/163hGFP was introduced into onion epidermal cells by the particle bombardment method with a PDS 1000/He. Transformed cells were incubated for 24 h at 22℃ in the dark and green fluorescence was monitored under a confocal microscope. The gene W17 was fused N-terminus of GST (glutathione-S-transferase) in prokaryotic expression vector pGEX-4T-1 and then transformed into E. coli strain BL21 (DE3). IPTG (0.5 mmol L-1) was added to induce the expression of recombinant GST/W17 for 3 h. The fused proteins were purified by GST purification columns, and then subjected to gel retardation assay with a 32p-labeled GCC or DRE sequence. The different reporter and effector plasmids were introduced into tobacco leaves through agroinfiltration, then transformed leaves stained by X-Gluc, faded with 75% alcohol and monitored under a Stereozooming microscope. The GFP fused with W17 protein was localized in the nuclei; SDS-PAGE assay demonstrated that the fused protein GST/W17 could be induced and purified with molecular weight at around 42.2 kD under the induction of IPTG. Purified fused protein was able to specifically bind to both the wild-type GCC-box and DRE element, but had no interaction with either the mutant DRE or GCC-box; W17 protein can bind to GCC-box and transactive downstream GUS gene in vivo. W17 can localize into the nuclei, and it may be involved not only in biotic stresses controlled by GCC-box, but also in abiotic stresses (e.g., salt-) induced signaling pathway.展开更多
The cluster-shaped plasmonic nanostructures are used to manage the incident light inside an ultra-thin silicon solar cell.Here we simulate spherical,conical,pyramidal,and cylindrical nanoparticles in a form of a clust...The cluster-shaped plasmonic nanostructures are used to manage the incident light inside an ultra-thin silicon solar cell.Here we simulate spherical,conical,pyramidal,and cylindrical nanoparticles in a form of a cluster at the rear side of a thin silicon cell,using the finite difference time domain(FDTD)method.By calculating the optical absorption and hence the photocurrent,it is shown that the clustering of nanoparticles significantly improves them.The photocurrent enhancement is the result of the plasmonic effects of clustering the nanoparticles.For comparison,first a cell with a single nanoparticle at the rear side is evaluated.Then four smaller nanoparticles are put around it to make a cluster.The photocurrents of 20.478 mA/cm2,23.186 mA/cm2,21.427 mA/cm2,and 21.243 mA/cm2 are obtained for the cells using clustering conical,spherical,pyramidal,cylindrical NPs at the backside,respectively.These values are 13.987 mA/cm2,16.901 mA/cm2,16.507 mA/cm2,17.926 mA/cm2 for the cell with one conical,spherical,pyramidal,cylindrical NPs at the backside,respectively.Therefore,clustering can significantly improve the photocurrents.Finally,the distribution of the electric field and the generation rate for the proposed structures are calculated.展开更多
BACKGROUND: As a member of the LIM protein family Ⅱ, cysteine- and glycine-rich protein-2 (CRP2) has been demonstrated to play a role in the regulation of growth and differentiation of eukaryotic cells. Our previo...BACKGROUND: As a member of the LIM protein family Ⅱ, cysteine- and glycine-rich protein-2 (CRP2) has been demonstrated to play a role in the regulation of growth and differentiation of eukaryotic cells. Our previous study has demonstrated that CRP2 can be detected in the embryonic rat inner ear but not in the adult rat inner ear. However, at present, the expression of LIM protein family H members in stem or precursor cells has not been described. OBJECTIVE: To determine the expression and sub-cellular localization of CRP2 in olfactory stem cells. DESIGN, TIME AND SETTING: An experiment with repeated measures was performed in the Laboratory of Otorhinolaryngology, Head and Neck Surgery, Xijing Hospital, the Fourth Military Medical University from February 2008 to April 2008. MATERIALS: Olfactory stem cells, and rabbit-anti-CRP2 polyclonal antibody were prepared and kept in our laboratory. METHODS: Reverse transcription polymerase chain reaction and Western blot analysis were used to detect expression of CRP2 in olfactory stem cells. Immunocytochemistry was also used to localize CRP2 in olfactory stem cells. MAIN OUTCOME MEASURES: The expression and sub-cellular localization of CRP2 in rat olfactory stem cells. RESULTS: CRP2 expression was found in olfactory stem cells, and CRP2 was distributed in both the nucleus and the cytoplasm. CONCLUSION: Confirmation of the expression and distribution of CRP2 in olfactory stem cells.展开更多
The meta-heuristic algorithm with local search is an excellent choice for the job-shop scheduling problem(JSP).However,due to the unique nature of the JSP,local search may generate infeasible neighbourhood solutions.I...The meta-heuristic algorithm with local search is an excellent choice for the job-shop scheduling problem(JSP).However,due to the unique nature of the JSP,local search may generate infeasible neighbourhood solutions.In the existing literature,although some domain knowledge of the JSP can be used to avoid infeasible solutions,the constraint conditions in this domain knowledge are sufficient but not necessary.It may lose many feasible solutions and make the local search inadequate.By analysing the causes of infeasible neighbourhood solutions,this paper further explores the domain knowledge contained in the JSP and proposes the sufficient and necessary constraint conditions to find all feasible neighbourhood solutions,allowing the local search to be carried out thoroughly.With the proposed conditions,a new neighbourhood structure is designed in this paper.Then,a fast calculation method for all feasible neighbourhood solutions is provided,significantly reducing the calculation time compared with ordinary methods.A set of standard benchmark instances is used to evaluate the performance of the proposed neighbourhood structure and calculation method.The experimental results show that the calculation method is effective,and the new neighbourhood structure has more reliability and superiority than the other famous and influential neighbourhood structures,where 90%of the results are the best compared with three other well-known neighbourhood structures.Finally,the result from a tabu search algorithm with the new neighbourhood structure is compared with the current best results,demonstrating the superiority of the proposed neighbourhood structure.展开更多
Nuclear proteins often form punctiform structures, but the precise mechanism for this process is unknown. As a preliminary study, we investigated the aggregation of an HSV-1 immediate-early protein, infected-cell prot...Nuclear proteins often form punctiform structures, but the precise mechanism for this process is unknown. As a preliminary study, we investigated the aggregation of an HSV-1 immediate-early protein, infected-cell protein 22 (ICP22), in the nucleus by observing the localization of ICP22-EGFP fusion protein Results showed that, in high-level expression conditions, ICP22-EGFP gradually concentrates in the nucleus, persists throughout the cell cycle without disaggregation even in the cell division phase, and is finally distributed to daughter cells. We subsequently constructed a mammalian cell expression system, which had tetracycline- dependent transcriptional regulators. Consequently, the location of ICP22-EGFP in the nucleus changed with distinct induction conditions. This suggests that the cellular location of ICP22 is also influenced by promoter regulation, in addition to its own structure. Our findings provide new clues for the investigation of transcriptional regulation of viral genes. In addition, the non-protease reporter system we constructed could be utilized to evaluate the role of intemal ribosome entry sites (IRES) on transcriptional regulation.展开更多
基金Project supported by the National Natural Science Foundation of China.
文摘The homotopy formulas of (r,s) differential forms and the solution of equation of type (r,s) on local q-convex domains in Stein manifolds are obtained.The homotopy formulas on local q-convex domains have important applications in uniform estimates of equation and holomorphic extension of CR-manifolds.
基金Projects(60234030 60404021) supported by the National Natural Science Foundation of China
文摘Immune evolutionary algorithms with domain knowledge were presented to solve the problem of simultaneous localization and mapping for a mobile robot in unknown environments. Two operators with domain knowledge were designed in algorithms, where the feature of parallel line segments without the problem of data association was used to construct a vaccination operator, and the characters of convex vertices in polygonal obstacle were extended to develop a pulling operator of key point grid. The experimental results of a real mobile robot show that the computational expensiveness of algorithms designed is less than other evolutionary algorithms for simultaneous localization and mapping and the maps obtained are very accurate. Because immune evolutionary algorithms with domain knowledge have some advantages, the convergence rate of designed algorithms is about 44% higher than those of other algorithms.
基金Project supported by the National Natural Science Foundation of China (No. 10377108)the Natural Science Foundation of Guangdong Province (No. 031495), China
文摘In this paper, we give the four equivalent characterizations for the weighted local hardy spaces on Lipschitz domains. Also, we give their application for the harmonic function defined in bounded Lipschitz domains.
基金the Swedish Institute, No. 00287/2006210the North Sweden Cancer Foundation, Specialized Research Fund for the Doctoral Program New Teacher of Higher Education by the Chinese Ministry of Education, No. 200804861039the National Natural Science Foundation of China, No. 30973073, 30973072
文摘The current study investigated correlations between the expression of leucine-rich repeats and immunoglobulin-like domain 1 (LRIG1) and antioxidant enzymes and related proteins, including manganese superoxide dismutase, glutamate cysteine ligase catalytic or regulatory subunit, thioredoxin and thioredoxin reductase, in both human ependymoma and oligodendroglioma. Results revealed that the cytoplasmic expression of LRIG1 was associated with expression of glutamate cysteine ligase catalytic subunit in the human ependymoma, while the nuclear expression of LRIG1 was associated with expression of thioredoxin reductase. In human oligodendroglioma, the cytoplasmic expression of LRIG1 was associated with expression of the glutamate cysteine ligase catalytic subunit. Both the nuclear and perinuclear expressions of LRIG1 were associated with expression of glutamate cysteine ligase regulatory subunit. These results indicated that several antioxidant enzymes and related proteins contributed to LRIG1 expression, and that these may participate in the antioxidation of the cells.
文摘It is proved that the global existence for the nonhomogeneous quasilinear wave equation with a localized weakly nonlinear dissipation in exterior domains.
文摘Let Ω be a bounded convex domain in Rn(n≥3) and G(x,y) be the Green function of the Laplace operator -△on Ω Let hPT(Ω) = {f∈D'(Ω) : (?)F ∈ hP(Rn), s.t. F|Ω = f}, by the atom characterization of Local Hardy spaces in a bounded Lipschitz domain, the bound of f Ω(?)2(Gf) for every f ∈hPr(Ω) is obtained, where n/(n + 1) <p≤1.
文摘Recent experiments revealed many new phenomena of the macroscopic domain patterns in the stress-induced phase transformation of a superelastic polycrystalline NiTi tube during tensile loading. The new phenomena include deformation instability with the formation of a helical domain, domain topology transition from helix to cylinder, domain-front branching and loading-path dependence of domain patterns. In this paper, we model the polycrystal as an elastic continuum with nonconvex strain energy and adopt the non-local strain gradient energy to account for the energy of the diffusive domain front. We simulate the equilibrium domain patterns and their evolution in the tubes under tensile loading by a non-local Finite Element Method (FEM). It is revealed that the observed loading-path dependence and topology transition of do- main patterns are due to the thermodynamic metastability of the tube system. The computation also shows that the tube-wall thickness has a significant effect on the domain patterns: with fixed material properties and interfacial energy density, a large tube-wall thickness leads to a long and slim helical domain and a severe branching of the cylindrical-domain front.
基金supported by the National Natural Science Foundation of China(Grant Nos.61331007 and 61471105)
文摘An efficient conformal locally one-dimensional finite-difference time-domain(LOD-CFDTD) method is presented for solving two-dimensional(2D) electromagnetic(EM) scattering problems. The formulation for the 2D transverse-electric(TE) case is presented and its stability property and numerical dispersion relationship are theoretically investigated. It is shown that the introduction of irregular grids will not damage the numerical stability. Instead of the staircasing approximation, the conformal scheme is only employed to model the curve boundaries, whereas the standard Yee grids are used for the remaining regions. As the irregular grids account for a very small percentage of the total space grids, the conformal scheme has little effect on the numerical dispersion. Moreover, the proposed method, which requires fewer arithmetic operations than the alternating-direction-implicit(ADI) CFDTD method, leads to a further reduction of the CPU time. With the total-field/scattered-field(TF/SF) boundary and the perfectly matched layer(PML), the radar cross section(RCS) of two2 D structures is calculated. The numerical examples verify the accuracy and efficiency of the proposed method.
文摘引入局部条件并半格 (简记为 L cusl)及其理想完备化等概念 .证明了 :任一代数 L domain的紧元集是 L cusl;任一代数 L domain是其紧元集赋予 Alexandrov拓扑时的 Sober化 ;任一 L cusl的理想完备化是代数 L domain,从而得到了代数 L domain的表示定理 .还证明了 Scott连续映射为态射的代数 L domain范畴为 L cusl与单调映射作成的范畴的反射子范畴 .
基金financially supported by the National 863 Program of China(2007AA10Z130)the National Natural Science Foundation of China(30700504).
文摘The study aims to detect the subcellular localization of ERF (ethylene-responsive element binding factor) transcription factor W17 protein, the interaction between W 17 and cis-acting regulatory elements GCC-box and DRE in vitro, the binding and transactivating ability in vivo, and the role of W17 in higher plant stress-signal pathway. Recombinant plasmid W17/163hGFP was introduced into onion epidermal cells by the particle bombardment method with a PDS 1000/He. Transformed cells were incubated for 24 h at 22℃ in the dark and green fluorescence was monitored under a confocal microscope. The gene W17 was fused N-terminus of GST (glutathione-S-transferase) in prokaryotic expression vector pGEX-4T-1 and then transformed into E. coli strain BL21 (DE3). IPTG (0.5 mmol L-1) was added to induce the expression of recombinant GST/W17 for 3 h. The fused proteins were purified by GST purification columns, and then subjected to gel retardation assay with a 32p-labeled GCC or DRE sequence. The different reporter and effector plasmids were introduced into tobacco leaves through agroinfiltration, then transformed leaves stained by X-Gluc, faded with 75% alcohol and monitored under a Stereozooming microscope. The GFP fused with W17 protein was localized in the nuclei; SDS-PAGE assay demonstrated that the fused protein GST/W17 could be induced and purified with molecular weight at around 42.2 kD under the induction of IPTG. Purified fused protein was able to specifically bind to both the wild-type GCC-box and DRE element, but had no interaction with either the mutant DRE or GCC-box; W17 protein can bind to GCC-box and transactive downstream GUS gene in vivo. W17 can localize into the nuclei, and it may be involved not only in biotic stresses controlled by GCC-box, but also in abiotic stresses (e.g., salt-) induced signaling pathway.
文摘The cluster-shaped plasmonic nanostructures are used to manage the incident light inside an ultra-thin silicon solar cell.Here we simulate spherical,conical,pyramidal,and cylindrical nanoparticles in a form of a cluster at the rear side of a thin silicon cell,using the finite difference time domain(FDTD)method.By calculating the optical absorption and hence the photocurrent,it is shown that the clustering of nanoparticles significantly improves them.The photocurrent enhancement is the result of the plasmonic effects of clustering the nanoparticles.For comparison,first a cell with a single nanoparticle at the rear side is evaluated.Then four smaller nanoparticles are put around it to make a cluster.The photocurrents of 20.478 mA/cm2,23.186 mA/cm2,21.427 mA/cm2,and 21.243 mA/cm2 are obtained for the cells using clustering conical,spherical,pyramidal,cylindrical NPs at the backside,respectively.These values are 13.987 mA/cm2,16.901 mA/cm2,16.507 mA/cm2,17.926 mA/cm2 for the cell with one conical,spherical,pyramidal,cylindrical NPs at the backside,respectively.Therefore,clustering can significantly improve the photocurrents.Finally,the distribution of the electric field and the generation rate for the proposed structures are calculated.
文摘BACKGROUND: As a member of the LIM protein family Ⅱ, cysteine- and glycine-rich protein-2 (CRP2) has been demonstrated to play a role in the regulation of growth and differentiation of eukaryotic cells. Our previous study has demonstrated that CRP2 can be detected in the embryonic rat inner ear but not in the adult rat inner ear. However, at present, the expression of LIM protein family H members in stem or precursor cells has not been described. OBJECTIVE: To determine the expression and sub-cellular localization of CRP2 in olfactory stem cells. DESIGN, TIME AND SETTING: An experiment with repeated measures was performed in the Laboratory of Otorhinolaryngology, Head and Neck Surgery, Xijing Hospital, the Fourth Military Medical University from February 2008 to April 2008. MATERIALS: Olfactory stem cells, and rabbit-anti-CRP2 polyclonal antibody were prepared and kept in our laboratory. METHODS: Reverse transcription polymerase chain reaction and Western blot analysis were used to detect expression of CRP2 in olfactory stem cells. Immunocytochemistry was also used to localize CRP2 in olfactory stem cells. MAIN OUTCOME MEASURES: The expression and sub-cellular localization of CRP2 in rat olfactory stem cells. RESULTS: CRP2 expression was found in olfactory stem cells, and CRP2 was distributed in both the nucleus and the cytoplasm. CONCLUSION: Confirmation of the expression and distribution of CRP2 in olfactory stem cells.
基金Supported by National Natural Science Foundation of China(Grant Nos.U21B2029 and 51825502).
文摘The meta-heuristic algorithm with local search is an excellent choice for the job-shop scheduling problem(JSP).However,due to the unique nature of the JSP,local search may generate infeasible neighbourhood solutions.In the existing literature,although some domain knowledge of the JSP can be used to avoid infeasible solutions,the constraint conditions in this domain knowledge are sufficient but not necessary.It may lose many feasible solutions and make the local search inadequate.By analysing the causes of infeasible neighbourhood solutions,this paper further explores the domain knowledge contained in the JSP and proposes the sufficient and necessary constraint conditions to find all feasible neighbourhood solutions,allowing the local search to be carried out thoroughly.With the proposed conditions,a new neighbourhood structure is designed in this paper.Then,a fast calculation method for all feasible neighbourhood solutions is provided,significantly reducing the calculation time compared with ordinary methods.A set of standard benchmark instances is used to evaluate the performance of the proposed neighbourhood structure and calculation method.The experimental results show that the calculation method is effective,and the new neighbourhood structure has more reliability and superiority than the other famous and influential neighbourhood structures,where 90%of the results are the best compared with three other well-known neighbourhood structures.Finally,the result from a tabu search algorithm with the new neighbourhood structure is compared with the current best results,demonstrating the superiority of the proposed neighbourhood structure.
基金The National Natural Science Foundation of China (30670094, 30700028)the Ph.D. Programs Foundation of Ministry of Education of China (2006-0023008)
文摘Nuclear proteins often form punctiform structures, but the precise mechanism for this process is unknown. As a preliminary study, we investigated the aggregation of an HSV-1 immediate-early protein, infected-cell protein 22 (ICP22), in the nucleus by observing the localization of ICP22-EGFP fusion protein Results showed that, in high-level expression conditions, ICP22-EGFP gradually concentrates in the nucleus, persists throughout the cell cycle without disaggregation even in the cell division phase, and is finally distributed to daughter cells. We subsequently constructed a mammalian cell expression system, which had tetracycline- dependent transcriptional regulators. Consequently, the location of ICP22-EGFP in the nucleus changed with distinct induction conditions. This suggests that the cellular location of ICP22 is also influenced by promoter regulation, in addition to its own structure. Our findings provide new clues for the investigation of transcriptional regulation of viral genes. In addition, the non-protease reporter system we constructed could be utilized to evaluate the role of intemal ribosome entry sites (IRES) on transcriptional regulation.