To wirelessly obtain the accurate location and orientation of an objective and exert an appropriate guidance for the objective, a feasible approach is to enclose a small rectangular permanent mag- net in the objective...To wirelessly obtain the accurate location and orientation of an objective and exert an appropriate guidance for the objective, a feasible approach is to enclose a small rectangular permanent mag- net in the objective. The magnetic field, produced by the rectangular magnet can be detected by magnetic sensors outside the objective. With these sensor data, the 3D localization and 3D orienta- tion parameters can be computed based on the mathematic model of the rectangular magnet magnetic field. In this 6D localization and orientation system, we first obtain 5D parameters of the objective by dipole model, then based on these parameters we can obtain 6D parameters by the model of rectangular magnet magnetic field using the particle swarm optimization （PSO） algorithm. Simulation experiments show that the proposed approach achieves ~ood performance.展开更多
“食品微生物学实验”课程是以微生物学为理论基础,培养学生运用微生物学实验技术和相关知识,对食品中的微生物进行检测、分析及运用的能力。为达到人才培养的目标,在成果导向教育(Outcome Based Education,简称OBE)理念的指导下对“食...“食品微生物学实验”课程是以微生物学为理论基础,培养学生运用微生物学实验技术和相关知识,对食品中的微生物进行检测、分析及运用的能力。为达到人才培养的目标,在成果导向教育(Outcome Based Education,简称OBE)理念的指导下对“食品微生物学实验”课程进行重构的探索。遵循成果导向教育教学反向设计的原则,通过用人单位、学生、同行等调研,根据毕业要求、调研结果及学生培养过程中实际存在问题,修订“食品微生物学实验”课程教学目标。根据课程目标,重构“食品微生物学实验”教学实验项目,根据教学的实验项目,更新教学组织方式,并探索适合“食品微生物学实验”的学习效果考核方式。通过教学实验项目、教学组织方式及学习考核方式的重构,以期最大化达到课程教学目标,从而培养具备专业知识、素养和能力的学生。展开更多
The 3D location and dipole orientation of light emitters provide essential information in many biological,chemical,and physical systems.Simultaneous acquisition of both information types typically requires pupil engin...The 3D location and dipole orientation of light emitters provide essential information in many biological,chemical,and physical systems.Simultaneous acquisition of both information types typically requires pupil engineering for 3D localization and dual-channel polarization splitting for orientation deduction.Here we report a geometric phase helical point spread function for simultaneously estimating the 3D position and dipole orientation of point emitters.It has a compact and simpler optical configuration compared to polarization-splitting techniques and yields achromatic phase modulation in contrast to pupil engineering based on dynamic phase,showing great potential for single-molecule orientation and localization microscopy.展开更多
文摘To wirelessly obtain the accurate location and orientation of an objective and exert an appropriate guidance for the objective, a feasible approach is to enclose a small rectangular permanent mag- net in the objective. The magnetic field, produced by the rectangular magnet can be detected by magnetic sensors outside the objective. With these sensor data, the 3D localization and 3D orienta- tion parameters can be computed based on the mathematic model of the rectangular magnet magnetic field. In this 6D localization and orientation system, we first obtain 5D parameters of the objective by dipole model, then based on these parameters we can obtain 6D parameters by the model of rectangular magnet magnetic field using the particle swarm optimization （PSO） algorithm. Simulation experiments show that the proposed approach achieves ~ood performance.
文摘“食品微生物学实验”课程是以微生物学为理论基础,培养学生运用微生物学实验技术和相关知识,对食品中的微生物进行检测、分析及运用的能力。为达到人才培养的目标,在成果导向教育(Outcome Based Education,简称OBE)理念的指导下对“食品微生物学实验”课程进行重构的探索。遵循成果导向教育教学反向设计的原则,通过用人单位、学生、同行等调研,根据毕业要求、调研结果及学生培养过程中实际存在问题,修订“食品微生物学实验”课程教学目标。根据课程目标,重构“食品微生物学实验”教学实验项目,根据教学的实验项目,更新教学组织方式,并探索适合“食品微生物学实验”的学习效果考核方式。通过教学实验项目、教学组织方式及学习考核方式的重构,以期最大化达到课程教学目标,从而培养具备专业知识、素养和能力的学生。
基金supported by the National Natural Science Foundation of China(Nos.62105368,62275268,and 62375284)the Science and Technology Innovation Program of Hunan Province(No.2023RC3010)。
文摘The 3D location and dipole orientation of light emitters provide essential information in many biological,chemical,and physical systems.Simultaneous acquisition of both information types typically requires pupil engineering for 3D localization and dual-channel polarization splitting for orientation deduction.Here we report a geometric phase helical point spread function for simultaneously estimating the 3D position and dipole orientation of point emitters.It has a compact and simpler optical configuration compared to polarization-splitting techniques and yields achromatic phase modulation in contrast to pupil engineering based on dynamic phase,showing great potential for single-molecule orientation and localization microscopy.
