Cytokinins(CKs),primarily trans-zeatin(tZ)and isopentenyladenine(iP)types,play critical roles in plant growth,development,and various stress responses.Long-distance transport of tZ-type CKs meidated by Arabidopsis ATP...Cytokinins(CKs),primarily trans-zeatin(tZ)and isopentenyladenine(iP)types,play critical roles in plant growth,development,and various stress responses.Long-distance transport of tZ-type CKs meidated by Arabidopsis ATP-binding cassette transporter subfamily G14(AtABCG14)has been well studied;however,less is known about the biochemical properties of AtABCG14 and its transporter activity toward iP-type CKs.Here we reveal the biochemical properties of AtABCG14 and provide evidence that it is also required for long-distance transport of iP-type CKs.AtABCG14 formed homodimers in human(Homo sapiens)HEK293T,tobacco(Nicotiana tabacum),and Arabidopsis cells.Transporter activity assays of AtABCG14 in Arabidopsis,tobacco,and yeast(Saccharomyces cerevisiae)showed that AtABCG14 may directly transport multiple CKs,including iP-and tZ-type species.AtABCG14 expression was induced by iP in a tZ-type CKdeficient double mutant(cypDM)of CYP735A1 and CYP735A2.The atabcg14 cypDM triple mutant exhibited stronger CK-deficiency phenotypes than cypDM.Hormone profiling,reciprocal grafting,and 2H6-iP isotope tracer experiments showed that root-to-shoot and shoot-to-root long-distance transport of iP-type CKs were suppressed in atabcg14 cypDM and atabcg14.These results suggest that AtABCG14 participates in three steps of the circular long-distance transport of iP-type CKs:xylem loading in the root for shootward transport,phloem unloading in the shoot for shoot distribution,and phloemunloading in the root for root distribution.We found that AtABCG14 displays transporter activity toward multiple CK species and revealed its versatile roles in circular long-distance transport of iP-type CKs.These findings provide newinsights into the transport mechanisms of CKs and other plant hormones.展开更多
Thousands of long-distance mobile mRNAs were identified from different grafting systems,based on high-throughput sequencing technology.Moreover,the long-distance delivery of RNAs was proved to involve multiple mechani...Thousands of long-distance mobile mRNAs were identified from different grafting systems,based on high-throughput sequencing technology.Moreover,the long-distance delivery of RNAs was proved to involve multiple mechanisms.Here,we analyzed the homology,motif,and tRNA-like structure(TLS)of long-distance mobile mRNAs identified by RNA-seq as well as the RNA-binding protein(RBP)in nine grafting combinations including Arabidopsis thaliana,Vitis vinifera,Cucumis sativus,Citrullus lanatus,Nicotiana benthamiana,Malus domestica,Pyrus spp.,Glycine max and Phaseolus vulgaris.Although several mRNAs were found to be shared in herbaceous,woody,and related species,the vast majority of long-distance mobile mRNAs were species-specific.Four non-specific movement-related motifs were identified,while the TLS was not necessary for mRNA long distance mobility.In addition,we found that RBPs were conserved among herbaceous and woody plants as well as related species.This paper reports a further in-depth analysis of the endogenous mechanisms by which the species-specific transportable m RNAs were selected by bioinformatics,in order to provide insights for future research on long-distance mobile mRNAs.展开更多
BELl-like transcription factors are ubiquitous in plants and interact with KNOTTEDI-types to regulate numerous developmental processes. In potato, the RNA of several BELl-like transcription factors has been identified...BELl-like transcription factors are ubiquitous in plants and interact with KNOTTEDI-types to regulate numerous developmental processes. In potato, the RNA of several BELl-like transcription factors has been identified in phloem cells. One of these, StBEL5, and its Knox protein partner regulate tuber formation by targeting genes that control growth. RNA detection methods and grafting experiments demonstrated that StBEL5 transcripts move across a graft union to localize in stolon tips, the site of tuber induction. This movement of RNA originates in source leaf veins and petioles and is induced by a short-day photoperiod, regulated by the untranslated regions, and correlated with enhanced tuber production. Addition of the StBEL5 untranslated regions to another BELl.like mRNA resulted in its preferential transport to stolon tips leading to increased tuber production. Upon fusion of the untranslated regions of StBEL5 to a β-glucuronidase marker, translation in tobacco protoplasts was repressed by those constructs containing the 3' untranslated sequence. The untranslated regions of the StBEL5 mRNA are involved in mediating its long-distance transport and in controlling translation. The 3' untranslated sequence contains an abundance of conserved motifs that may serve as binding motifs for RNA-binding proteins. Because of their presence in the phloem sieve tube system, their unique untranslated region sequences and their diverse RNA accumulation patterns, the family of BEL1-like RNAs from potato represents a valuable model for studying the long-distance transport of full-length mRNAs and their role in development.展开更多
Nicotinamide adenine dinucleotide (NAD) biosynthesis, including synthesis from aspartate via the de novo pathway and from nicotinate (NA) via the Preiss-Handler pathway, is conserved in land plants. Diverse spe-ci...Nicotinamide adenine dinucleotide (NAD) biosynthesis, including synthesis from aspartate via the de novo pathway and from nicotinate (NA) via the Preiss-Handler pathway, is conserved in land plants. Diverse spe-cies of NA conjugates, which are mainly involved in NA detoxification, were also found in all tested land plants. Among these conjugates, MeNA (NA methyl ester) has been widely detected in angiosperm plants, although its physiological function and the underlying mechanism for its production in planta remain largely unknown. Here, we show that MeNA is an NAD precursor undergoing more efficient long-distance trans-port between organs than NA and nicotinamide in Arabidopsis. We found that Arabidopsis has one meth- yltransferase (designated AtNaMT1) capable of catalyzing carboxyl methylation of NA to yield MeNA and one methyl esterase (MES2) predominantly hydrolyzing MeNA back to NA. We further uncovered that the transfer of [^14C]MeNA from the root to leaf was significantly increased in both MES2 knockdown and NaMTl-overexpressing lines, suggesting that both NaMT1 and MES2 fine-tune the long-distance transport of MeNA, which is ultimately utilized for NAD production. Abiotic stress (salt, abscisic acid, and mannitol) treatments, which are known to exacerbate NAD degradation, induce the expression of NaMT1 but sup-press MES2 expression, suggesting that MeNA may play a role in stress adaption. Collectively, our study indicates that reversible methylation of NA controls the biosynthesis of MeNA in Arabidopsis, which pre-sumably functions as a detoxification form of free NA for efficient long-distance transport and eventually NAD production especially under abiotic stress, providing new insights into the relationship between NAD biosynthesis and NA conjugation in plants.展开更多
For a water supply system with long-distance diversion pipelines, in addition to the water hammer problems that occur beyond pumps, the safety of the water diversion pipeline in front of pumps also deserves attention....For a water supply system with long-distance diversion pipelines, in addition to the water hammer problems that occur beyond pumps, the safety of the water diversion pipeline in front of pumps also deserves attention. In this study, a water hammer protection scheme combined with an overflow surge tank and a regulating valve was developed. A mathematical model of the overflow surge tank was developed, and an analytical formula for the height of the overflow surge tank was derived. Furthermore, a practical water supply project was used to evaluate the feasibility of the combined protection scheme and analyze the sensitivity of valve regulation rules. The results showed that the combined protection scheme effectively reduced the height of the surge tank, lessened the difficulties related to construction, and reduced the necessary financial investment for the project. The two-stage closing rule articulated as fast first and then slow could minimize the overflow volume of the surge tank when the power failure occurred, while the two-stage opening rule articulated as slow first and then fast could be more conducive to the safety of the water supply system when the pump started up.展开更多
Iron(Fe)transport and reallocation are essential to Fe homeostasis in plants,but it is unclear how Fe homeostasis is regulated,especially under stress.Here we report that NPF5.9 and its close homolog NPF5.8 redundantl...Iron(Fe)transport and reallocation are essential to Fe homeostasis in plants,but it is unclear how Fe homeostasis is regulated,especially under stress.Here we report that NPF5.9 and its close homolog NPF5.8 redundantly regulate Fe transport and reallocation in Arabidopsis.NPF5.9 is highly upregulated in response to Fe deficiency.NPF5.9 expresses preferentially in vasculature tissues and localizes to the trans-Golgi network,and NPF5.8 showed a similar expression pattern.Long-distance Fe transport and allocation into aerial parts was significantly increased in NPF5.9-overexpressing lines.In the double mutant npf5.8 npf5.9,Fe loading in aerial parts and plant growth were decreased,which were partially rescued by Fe supplementation.Further analysis showed that expression of PYE,the negative regulator for Fe homeostasis,and its downstream target NAS4 were significantly altered in the double mutant.NPF5.9 and NPF5.8 were shown to also mediate nitrate uptake and transport,although nitrate and Fe application did not reciprocally affect each other.Our findings uncovered the novel function of NPF5.9 and NPF5.8 in long-distance Fe transport and homeostasis,and further indicated that they possibly mediate nitrate transport and Fe homeostasis independently in Arabidopsis.展开更多
In plants,the lysine and histidine transporter(LHT)family represent a class of proteins that mediate the uptake,translocation,and utilization of amino acids.The tea plant(Camellia sinensis)is a perennial evergreen wit...In plants,the lysine and histidine transporter(LHT)family represent a class of proteins that mediate the uptake,translocation,and utilization of amino acids.The tea plant(Camellia sinensis)is a perennial evergreen with a relatively high level of amino acids.However,systematic identification and molecular characterization of the LHT gene family has rarely been reported in tea plants.In this study,22 CsLHTs were identified from the‘Shuchazao’genome and classified into two groups.The modeled three-dimensional structure and the conserved domains presented a high similarity among the LHTs proteins.Moreover,it was predicted that a few genes were conserved through the analysis of the physiochemical characters,structures and cis-elements in promoters.The expression patterns in tea plants revealed that CsLHT7 was mainly expressed in the roots,and CsLHT4 and CsLHT11 exhibited relatively high expression in both the roots and leaves.Moreover,the expression of all three genes could be induced by organic nitrogen.Additionally,heterogeneous expression of CsLHT4,CsLHT7 and CsLHT11 in Arabidopsis thaliana decreased the aerial parts biomass compared with that in WT plants while significantly increased the rosette biomass only for CsLHT11transgenic plants versus WT plants.Overall,our results provide fundamental information about CsLHTs and potential genes in N utilization for further analysis in tea plants.展开更多
In this study,the Weather Research and Forecasting(WRF)model and meteorological observation data were used to research the long-distance moisture transport supply source of the extreme rainfall event that occurred on ...In this study,the Weather Research and Forecasting(WRF)model and meteorological observation data were used to research the long-distance moisture transport supply source of the extreme rainfall event that occurred on July 21,2012 in Beijing.Recording a maximum rainfall amount of 460 mm in 24 h,this rainstorm event had two dominant moisture transport channels.In the early stage of the rainstorm,the first channel comprised southwesterly monsoonal moisture from the Bay of Bengal(BOB)that was directly transported to north China along the eastern edge of Tibetan Plateau(TP)by orographic uplift.During the rainstorm,the southwesterly moisture transport was weakened by the transfer of Typhoon Vicente.Moreover,the southeasterly moisture transport between the typhoon and western Pacific subtropical high(WPSH)became another dominant moisture transport channel.The moisture in the lower troposphere was mainly associated with the southeasterly moisture transport from the South China Sea and the East China Sea,and the moisture in the middle troposphere was mainly transported from the BOB and Indian Ocean.The control experiment well reproduced the distribution and intensity of rainfall and moisture transport.By comparing the control and three sensitivity experiments,we found that the moisture transported from Typhoon Vicente and a tropical cyclone in the BOB both significantly affected this extreme rainfall event.After Typhoon Vicente was removed in a sensitivity experiment,the maximum 24-h accumulated rainfall in north China was reduced by approximately 50%compared with that of the control experiment,while the rainfall after removing the tropical cyclone was reduced by 30%.When both the typhoon and tropical cyclone were removed,the southwesterly moisture transport was enhanced.Moreover,the sensitivity experiment of removing Typhoon Vicente also weakened the tropical cyclone in the BOB.Thus,the moisture pump driven by Typhoon Vicente played an important role in maintaining and strengthening the tropical cyclone in the BOB through its westerly airflow.Typhoon Vicente was not only the moisture transfer source for the southwesterly monsoonal moisture but also affected the tropical cyclone in the BOB,which was a key supply source of long-distance moisture transport for the extreme rainfall event on July21,2012 in Beijing.展开更多
The inward particle transport is associated with the formation of peaked density profiles,which contributes to improve the fusion rate and the realization of steady-state discharge.The active control of inward particl...The inward particle transport is associated with the formation of peaked density profiles,which contributes to improve the fusion rate and the realization of steady-state discharge.The active control of inward particle transport is considered as one of the most critical issues of magnetic confinement fusion.Recently,it is realized preliminarily by adding a biased endplate in the Peking University Plasma Test(PPT)device.The results reveal that the inward particle flux increases with the bias voltage of the endplate.It is also found that the profile of radial electric field(Er)shear is flattened by the increased bias voltage.Radial velocity fluctuations affect the inward particle more than density fluctuations,and the frequency of the dominant mode driving inward particle flux increases with the biased voltage applied to the endplate.The experimental results in the PPT device provide a method to actively control the inward particle flux using a biased endplate and enrich the understanding of the relationship between E_(r)×B shear and turbulence transport.展开更多
At the EAST tokamak, the ion temperature(T_(i)) is observed to be clamped around 1.25 keV in electron cyclotron resonance(ECR)-heated plasmas, even at core electron temperatures up to 10 keV(depending on the ECR heati...At the EAST tokamak, the ion temperature(T_(i)) is observed to be clamped around 1.25 keV in electron cyclotron resonance(ECR)-heated plasmas, even at core electron temperatures up to 10 keV(depending on the ECR heating power and the plasma density). This clamping results from the lack of direct ion heating and high levels of turbulence-driven transport. Turbulent transport analysis shows that trapped electron mode and electron temperature gradient-driven modes are the most unstable modes in the core of ECR-heated H-mode plasmas. Nevertheless, recently it was found that the T_(i)/T_(e)ratio can increase further with the fraction of the neutral beam injection(NBI) power, which leads to a higher core ion temperature(Ti0). In NBI heating-dominant H-mode plasmas, the ion temperature gradient-driven modes become the most unstable modes.Furthermore, a strong and broad internal transport barrier(ITB) can form at the plasma core in high-power NBI-heated H-mode plasmas when the T_(i)/T_(e)ratio approaches ~1, which results in steep core Teand Tiprofiles, as well as a peaked neprofile. Power balance analysis shows a weaker Teprofile stiffness after the formation of ITBs in the core plasma region, where Ticlamping is broken,and the core Tican increase further above 2 keV, which is 80% higher than the value of Ticlamping in ECR-heated plasmas. This finding proposes a possible solution to the problem of Ticlamping on EAST and demonstrates an advanced operational regime with the formation of a strong and broad ITB for future fusion plasmas dominated by electron heating.展开更多
With the depletion of fossil fuels and the demand for high-performance energy storage devices,solidstate lithium metal batteries have received widespread attention due to their high energy density and safety advantage...With the depletion of fossil fuels and the demand for high-performance energy storage devices,solidstate lithium metal batteries have received widespread attention due to their high energy density and safety advantages.Among them,the earliest developed organic solid-state polymer electrolyte has a promising future due to its advantages such as good mechanical flexibility,but its poor ion transport performance dramatically limits its performance improvement.Therefore,single-ion conducting polymer electrolytes(SICPEs)with high lithium-ion transport number,capable of improving the concentration polarization and inhibiting the growth of lithium dendrites,have been proposed,which provide a new direction for the further development of high-performance organic polymer electrolytes.In view of this,lithium ions transport mechanisms and design principles in SICPEs are summarized and discussed in this paper.The modification principles currently used can be categorized into the following three types:enhancement of lithium salt anion-polymer interactions,weakening of lithium salt anion-cation interactions,and modulation of lithium ion-polymer interactions.In addition,the advances in single-ion conductors of conventional and novel polymer electrolytes are summarized,and several typical highperformance single-ion conductors are enumerated and analyzed in what way they improve ionic conductivity,lithium ions mobility,and the ability to inhibit lithium dendrites.Finally,the advantages and design methodology of SICPEs are summarized again and the future directions are outlined.展开更多
The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain functio...The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with emotions.Specifically, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal mice.After knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was obviously mitigated. Additionally, administration of an excitatory amino acid transporter 2 inhibitor in the basolateral amygdala yielded a notable reduction in anxiety level among mice subjected to stress. These results suggest that basolateral amygdala astrocytic excitatory amino acid transporter 2 plays a role in in the regulation of unpredictable chronic mild stress-induced anxiety-like behavior by impacting the activity of local glutamatergic neurons, and targeting excitatory amino acid transporter 2 in the basolateral amygdala holds therapeutic promise for addressing anxiety disorders.展开更多
The dynamics of long-wavelength(kθ<1.4 cm^(-1)),broadband(20 kHz–200 kHz)electron temperature fluctuations(Te/Te)of plasmas in gas-puff experiments are observed for the first time in HL-2A tokamak.In a relatively...The dynamics of long-wavelength(kθ<1.4 cm^(-1)),broadband(20 kHz–200 kHz)electron temperature fluctuations(Te/Te)of plasmas in gas-puff experiments are observed for the first time in HL-2A tokamak.In a relatively low density(ne(0)■0.91×10^(19)m^(-3)–1.20×10^(19)m^(-3))scenario,after gas-puffing the core temperature increases and the edge temperature drops.On the contrary,temperature fluctuation drops at the core and increases at the edge.Analyses show the non-local emergence is accompanied with a long radial coherent length of turbulent fluctuations.While in a higher density(ne(0)?1.83×10^(19)m^(-3)–2.02×10^(19)m^(-3))scenario,the phenomena are not observed.Furthermore,compelling evidence indicates that E×B shear serves as a substantial contributor to this extensive radial interaction.This finding offers a direct explanatory link to the intriguing core-heating phenomenon witnessed within the realm of non-local transport.展开更多
Bismuthene,an inherently hexagonal structure characterized by a huge bulk gap,offers a versatile platform for investigating the electronic transport of various topological quantum states.Using nonequilibrium Green'...Bismuthene,an inherently hexagonal structure characterized by a huge bulk gap,offers a versatile platform for investigating the electronic transport of various topological quantum states.Using nonequilibrium Green's function method and Landauer-Büttiker formula,we thoroughly investigate the transport properties of various Hall-type quantum states,including quantum spin Hall(QSH)edge states,quantum valley Hall kink(QVHK)states,and quantum spin-valley Hall kink(QSVHK)states,in the presence of various disorders.Based on the exotic transport features,a spin-valley filter,capable of generating a highly spin-and valley-polarized current,is proposed.The valley index and the spin index of the filtered QSVHK state are determined by the staggered potential and the intrinsic spin-orbit coupling,respectively.The efficiency of the spin-valley filter is supported by the spacial current distribution,the valley-resolved conductance,and the spin-resolved conductance.Compared with a sandwich structure for QSVHK,our proposed spin-valley filter can work with a much smaller size and is more accessible in the experiment.展开更多
The wadi dahab delta is in a dry, arid coastal zone within Egypt’s south Sinai Peninsula’s eastern portion. The primary water source is the Quaternary coastal alluvial aquifer. The groundwater salinity varies from 8...The wadi dahab delta is in a dry, arid coastal zone within Egypt’s south Sinai Peninsula’s eastern portion. The primary water source is the Quaternary coastal alluvial aquifer. The groundwater salinity varies from 890to 8213 mg/L, with a mean value of 3417 mg/L. The dissolved major ions have been used to calculate the seawater mixing index(SWMI) using a linear equation that discriminates the groundwater mostly affected by water–rock interaction(SWMI 1>) and other samples mixed with Seawater(SWMI < 1). The isotopic composition of groundwater for specifically chosen groundwater samples ranges from-0.645‰ to +5.212‰ for δ^(18)O and from-9.582‰ to + 22.778‰ for δ^(2)H, where the seawater represented by a Red Sea water sample(δ^(18)O + 1.64‰-δ^(2)H + 9.80‰) and reject brine water are considerably enriched the isotopic groundwater values. The geochemical NETPATH model constrained by the dissolved significant ions, isotopes, and the rock aquifer forming minerals as phases indicate the mixing percent with the seawater ranges from 9% to 97% of seawater from 91% to 3% of original recharge water. According to the SEAWAT 3-D flow models, seawater has penetrated the Northeastern Dahab delta aquifer, with the intrusion zone extending1500 m inland. The salt dissolution, upwelling of saline water, recharge from the upstream mountain block, and seawater encroachment are the primary aspects contributing to the deterioration of groundwater quality. These findings may have significance for effective groundwater withdrawal management in arid locations worldwide with similar hydrogeological systems.展开更多
Variations in the nutrients and water that plants require for metabolism,development,and the maintenance of cellular homeostasis are the main causes of abiotic stress in plants.It has,however,hardly ever been studied ...Variations in the nutrients and water that plants require for metabolism,development,and the maintenance of cellular homeostasis are the main causes of abiotic stress in plants.It has,however,hardly ever been studied how these transporter proteins,such as aquaporin which is responsible for food and water intake in cell plasma mem-branes,interact with one another.This review aims to explore the interactions between nutrient transporters and aquaporins during water and nutrient uptake.It also investigates how symbiotic relationships influence the plant genome’s responses to regulatory processes such as photoperiodism,senescence,and nitrogenfixation.These responses are observed in reaction to various abiotic stresses.For instance,plasma membrane transporters are upregulated during macronutrient insufficiency,tonoplast transporters are overexpressed,and aquaporins are downregulated in micronutrient deficiency.Additionally,tolerant plants often exhibit increased expression of nutrient transporters and aquaporins in response to drought,salt,and cold temperatures.To better comprehend plant stress tolerance to abiotic challenges including starvation,K famine,salt,and freezing temperatures,both classes of nutrient and water transporters should be considered at the same time.展开更多
Controlling mass transportation using intrinsic mechanisms is a challenging topic in nanotechnology.Herein,we employ molecular dynamics simulations to investigate the mass transport inside carbon nanotubes(CNT)with te...Controlling mass transportation using intrinsic mechanisms is a challenging topic in nanotechnology.Herein,we employ molecular dynamics simulations to investigate the mass transport inside carbon nanotubes(CNT)with temperature gradients,specifically the effects of adding a static carbon hoop to the outside of a CNT on the transport of a nanomotor inside the CNT.We reveal that the underlying mechanism is the uneven potential energy created by the hoops,i.e.,the hoop outside the CNT forms potential energy barriers or wells that affect mass transport inside the CNT.This fundamental control of directional mass transportation may lead to promising routes for nanoscale actuation and energy conversion.展开更多
Due to the lack of inversion,mirror or other roto-inversion symmetries,chiral crystals possess a well-defined handedness which,when combined with time-reversal symmetry breaking from the application of magnetic fields...Due to the lack of inversion,mirror or other roto-inversion symmetries,chiral crystals possess a well-defined handedness which,when combined with time-reversal symmetry breaking from the application of magnetic fields,can give rise to directional dichroism of the electrical transport phenomena via the magnetochiral anisotropy.In this study,we investigate the nonreciprocal magneto-transport in microdevices of NbGe_(2),a superconductor with structural chirality.A giant nonreciprocal signal from vortex motions is observed during the superconducting transition,with the ratio of nonreciprocal resistance to the normal resistanceγreaching 6×10^(5)T^(-1)·A^(-1).Interestingly,the intensity can be adjusted and even sign-reversed by varying the current,the temperature,and the crystalline orientation.Our findings illustrate intricate vortex dynamics and offer ways of manipulation on the rectification effect in superconductors with structural chirality.展开更多
Leaf color mutants in higher plants are considered to be ideal materials for studying the chlorophyll biosynthesis,photosynthesis mechanism and chloroplast development.Herein,we identified a spontaneous mutant,yc412,i...Leaf color mutants in higher plants are considered to be ideal materials for studying the chlorophyll biosynthesis,photosynthesis mechanism and chloroplast development.Herein,we identified a spontaneous mutant,yc412,in cultivated cucumber that exhibited yellow cotyledons.The yellow-lethal mutant was diagnosed with an abnormal chloroplast ultrastructure,and reduced photosynthetic capacity and pigment content.Through bulked segregant analysis-based whole-genome sequencing and fine genetic mapping,we narrowed the yellow cotyledons (yc) locus to a 96.8 kb interval on chromosome 3.By resequencing and molecular cloning,we showed that Csyc is a potential candidate gene,which encodes a yellow stripe-like (YSL) transporter.The T to C mutation in the promoter region of Csyc caused the yellow cotyledon phenotype in yc412.Compared to YZU027A (WT),the expression of Csyc was significantly downregulated in the cotyledons of yc412.Silencing of Csyc in cucumber via virus-induced gene silencing resulted in chlorotic leaves,mainly by suppressing the chlorophyll content.Furthermore,a comparative transcriptome analysis revealed that chloroplast-related genes and chlorophyll biosynthesis genes were significantly downregulated in yc412 cotyledons.Our results provide new insights into the molecular function of the YSL transporter in plant chloroplast development and chlorophyll synthesis.展开更多
Deepsea mining has been proposed since the 1960s to alleviate the lack of resources on land.Vertical hydraulic transport of collected ores from the seabed to the sea surface is considered the most promising method for...Deepsea mining has been proposed since the 1960s to alleviate the lack of resources on land.Vertical hydraulic transport of collected ores from the seabed to the sea surface is considered the most promising method for industrial applications.In the present study,an indoor model test of the vertical hydraulic transport of particles was conducted.A noncontact optical method has been proposed to measure the local characteristics of the particles inside a vertical pipe,including the local concentration and particle velocity.The hydraulic gradient of ore transport was evaluated with various particle size distributions,particle densities,feeding concentrations and mixture flow velocities.During transport,the local concentration is larger than the feeding concentration,whereas the particle velocity is less than the mixture velocity.The qualitative effects of the local concentration and local fluid velocity on the particle velocity and slip velocity were investigated.The local fluid velocity contributes significantly to particle velocity and slip velocity,whereas the effect of the local concentration is marginal.A higher feeding concentration and mixture flow velocity result in an increased hydraulic gradient.The effect of the particle size gradation is slight,whereas the particle density plays a crucial role in the transport.展开更多
基金the National Natural Science Foundation of China(31470370 to K.Z.and 32100270 to J.Z.)the Natural Science Foundation of Zhejiang Province(LY22C020003 to J.Z.)C.-J.L.was supported by the DOE Office of Basic Energy Sciences,specifically through the Physical Biosciences Program of the Chemical Sciences,Geosciences and Biosciences Division,under contract DESC0012704.
文摘Cytokinins(CKs),primarily trans-zeatin(tZ)and isopentenyladenine(iP)types,play critical roles in plant growth,development,and various stress responses.Long-distance transport of tZ-type CKs meidated by Arabidopsis ATP-binding cassette transporter subfamily G14(AtABCG14)has been well studied;however,less is known about the biochemical properties of AtABCG14 and its transporter activity toward iP-type CKs.Here we reveal the biochemical properties of AtABCG14 and provide evidence that it is also required for long-distance transport of iP-type CKs.AtABCG14 formed homodimers in human(Homo sapiens)HEK293T,tobacco(Nicotiana tabacum),and Arabidopsis cells.Transporter activity assays of AtABCG14 in Arabidopsis,tobacco,and yeast(Saccharomyces cerevisiae)showed that AtABCG14 may directly transport multiple CKs,including iP-and tZ-type species.AtABCG14 expression was induced by iP in a tZ-type CKdeficient double mutant(cypDM)of CYP735A1 and CYP735A2.The atabcg14 cypDM triple mutant exhibited stronger CK-deficiency phenotypes than cypDM.Hormone profiling,reciprocal grafting,and 2H6-iP isotope tracer experiments showed that root-to-shoot and shoot-to-root long-distance transport of iP-type CKs were suppressed in atabcg14 cypDM and atabcg14.These results suggest that AtABCG14 participates in three steps of the circular long-distance transport of iP-type CKs:xylem loading in the root for shootward transport,phloem unloading in the shoot for shoot distribution,and phloemunloading in the root for root distribution.We found that AtABCG14 displays transporter activity toward multiple CK species and revealed its versatile roles in circular long-distance transport of iP-type CKs.These findings provide newinsights into the transport mechanisms of CKs and other plant hormones.
基金supported by the 111 Project(Grant No.B17043)the 2115 Talent Development Program of China Agricultural University。
文摘Thousands of long-distance mobile mRNAs were identified from different grafting systems,based on high-throughput sequencing technology.Moreover,the long-distance delivery of RNAs was proved to involve multiple mechanisms.Here,we analyzed the homology,motif,and tRNA-like structure(TLS)of long-distance mobile mRNAs identified by RNA-seq as well as the RNA-binding protein(RBP)in nine grafting combinations including Arabidopsis thaliana,Vitis vinifera,Cucumis sativus,Citrullus lanatus,Nicotiana benthamiana,Malus domestica,Pyrus spp.,Glycine max and Phaseolus vulgaris.Although several mRNAs were found to be shared in herbaceous,woody,and related species,the vast majority of long-distance mobile mRNAs were species-specific.Four non-specific movement-related motifs were identified,while the TLS was not necessary for mRNA long distance mobility.In addition,we found that RBPs were conserved among herbaceous and woody plants as well as related species.This paper reports a further in-depth analysis of the endogenous mechanisms by which the species-specific transportable m RNAs were selected by bioinformatics,in order to provide insights for future research on long-distance mobile mRNAs.
基金supported by the United States Departmentof Agriculture NRI-CGP award no.2008-02806National Science Foundation-Plant Genome Research Program award no.0820659
文摘BELl-like transcription factors are ubiquitous in plants and interact with KNOTTEDI-types to regulate numerous developmental processes. In potato, the RNA of several BELl-like transcription factors has been identified in phloem cells. One of these, StBEL5, and its Knox protein partner regulate tuber formation by targeting genes that control growth. RNA detection methods and grafting experiments demonstrated that StBEL5 transcripts move across a graft union to localize in stolon tips, the site of tuber induction. This movement of RNA originates in source leaf veins and petioles and is induced by a short-day photoperiod, regulated by the untranslated regions, and correlated with enhanced tuber production. Addition of the StBEL5 untranslated regions to another BELl.like mRNA resulted in its preferential transport to stolon tips leading to increased tuber production. Upon fusion of the untranslated regions of StBEL5 to a β-glucuronidase marker, translation in tobacco protoplasts was repressed by those constructs containing the 3' untranslated sequence. The untranslated regions of the StBEL5 mRNA are involved in mediating its long-distance transport and in controlling translation. The 3' untranslated sequence contains an abundance of conserved motifs that may serve as binding motifs for RNA-binding proteins. Because of their presence in the phloem sieve tube system, their unique untranslated region sequences and their diverse RNA accumulation patterns, the family of BEL1-like RNAs from potato represents a valuable model for studying the long-distance transport of full-length mRNAs and their role in development.
文摘Nicotinamide adenine dinucleotide (NAD) biosynthesis, including synthesis from aspartate via the de novo pathway and from nicotinate (NA) via the Preiss-Handler pathway, is conserved in land plants. Diverse spe-cies of NA conjugates, which are mainly involved in NA detoxification, were also found in all tested land plants. Among these conjugates, MeNA (NA methyl ester) has been widely detected in angiosperm plants, although its physiological function and the underlying mechanism for its production in planta remain largely unknown. Here, we show that MeNA is an NAD precursor undergoing more efficient long-distance trans-port between organs than NA and nicotinamide in Arabidopsis. We found that Arabidopsis has one meth- yltransferase (designated AtNaMT1) capable of catalyzing carboxyl methylation of NA to yield MeNA and one methyl esterase (MES2) predominantly hydrolyzing MeNA back to NA. We further uncovered that the transfer of [^14C]MeNA from the root to leaf was significantly increased in both MES2 knockdown and NaMTl-overexpressing lines, suggesting that both NaMT1 and MES2 fine-tune the long-distance transport of MeNA, which is ultimately utilized for NAD production. Abiotic stress (salt, abscisic acid, and mannitol) treatments, which are known to exacerbate NAD degradation, induce the expression of NaMT1 but sup-press MES2 expression, suggesting that MeNA may play a role in stress adaption. Collectively, our study indicates that reversible methylation of NA controls the biosynthesis of MeNA in Arabidopsis, which pre-sumably functions as a detoxification form of free NA for efficient long-distance transport and eventually NAD production especially under abiotic stress, providing new insights into the relationship between NAD biosynthesis and NA conjugation in plants.
基金supported by the National Natural Science Foundation of China(Grants No.52179062 and 51879087).
文摘For a water supply system with long-distance diversion pipelines, in addition to the water hammer problems that occur beyond pumps, the safety of the water diversion pipeline in front of pumps also deserves attention. In this study, a water hammer protection scheme combined with an overflow surge tank and a regulating valve was developed. A mathematical model of the overflow surge tank was developed, and an analytical formula for the height of the overflow surge tank was derived. Furthermore, a practical water supply project was used to evaluate the feasibility of the combined protection scheme and analyze the sensitivity of valve regulation rules. The results showed that the combined protection scheme effectively reduced the height of the surge tank, lessened the difficulties related to construction, and reduced the necessary financial investment for the project. The two-stage closing rule articulated as fast first and then slow could minimize the overflow volume of the surge tank when the power failure occurred, while the two-stage opening rule articulated as slow first and then fast could be more conducive to the safety of the water supply system when the pump started up.
基金R&D Program of China(2016YFD0100700)in part by the Ministry of Agriculture of China for Transgenic Research(2016ZX08009003-005-003).
文摘Iron(Fe)transport and reallocation are essential to Fe homeostasis in plants,but it is unclear how Fe homeostasis is regulated,especially under stress.Here we report that NPF5.9 and its close homolog NPF5.8 redundantly regulate Fe transport and reallocation in Arabidopsis.NPF5.9 is highly upregulated in response to Fe deficiency.NPF5.9 expresses preferentially in vasculature tissues and localizes to the trans-Golgi network,and NPF5.8 showed a similar expression pattern.Long-distance Fe transport and allocation into aerial parts was significantly increased in NPF5.9-overexpressing lines.In the double mutant npf5.8 npf5.9,Fe loading in aerial parts and plant growth were decreased,which were partially rescued by Fe supplementation.Further analysis showed that expression of PYE,the negative regulator for Fe homeostasis,and its downstream target NAS4 were significantly altered in the double mutant.NPF5.9 and NPF5.8 were shown to also mediate nitrate uptake and transport,although nitrate and Fe application did not reciprocally affect each other.Our findings uncovered the novel function of NPF5.9 and NPF5.8 in long-distance Fe transport and homeostasis,and further indicated that they possibly mediate nitrate transport and Fe homeostasis independently in Arabidopsis.
基金supported by the National Key Research and Development Program of China(Grant No.2018YFD1000600)the National Natural Science Foundation of China(Grant No.32070376)。
文摘In plants,the lysine and histidine transporter(LHT)family represent a class of proteins that mediate the uptake,translocation,and utilization of amino acids.The tea plant(Camellia sinensis)is a perennial evergreen with a relatively high level of amino acids.However,systematic identification and molecular characterization of the LHT gene family has rarely been reported in tea plants.In this study,22 CsLHTs were identified from the‘Shuchazao’genome and classified into two groups.The modeled three-dimensional structure and the conserved domains presented a high similarity among the LHTs proteins.Moreover,it was predicted that a few genes were conserved through the analysis of the physiochemical characters,structures and cis-elements in promoters.The expression patterns in tea plants revealed that CsLHT7 was mainly expressed in the roots,and CsLHT4 and CsLHT11 exhibited relatively high expression in both the roots and leaves.Moreover,the expression of all three genes could be induced by organic nitrogen.Additionally,heterogeneous expression of CsLHT4,CsLHT7 and CsLHT11 in Arabidopsis thaliana decreased the aerial parts biomass compared with that in WT plants while significantly increased the rosette biomass only for CsLHT11transgenic plants versus WT plants.Overall,our results provide fundamental information about CsLHTs and potential genes in N utilization for further analysis in tea plants.
基金National Natural Science Foundation of China(91937301,42030611)Second Tibetan Plateau Scientific Expedition and Research Program(2019QZKK0103,2019QZKK0105)Scientific and Technological Research Program of China Railway Eryuan Engineering Group CO.LTD(KYY2020066(20-22))
文摘In this study,the Weather Research and Forecasting(WRF)model and meteorological observation data were used to research the long-distance moisture transport supply source of the extreme rainfall event that occurred on July 21,2012 in Beijing.Recording a maximum rainfall amount of 460 mm in 24 h,this rainstorm event had two dominant moisture transport channels.In the early stage of the rainstorm,the first channel comprised southwesterly monsoonal moisture from the Bay of Bengal(BOB)that was directly transported to north China along the eastern edge of Tibetan Plateau(TP)by orographic uplift.During the rainstorm,the southwesterly moisture transport was weakened by the transfer of Typhoon Vicente.Moreover,the southeasterly moisture transport between the typhoon and western Pacific subtropical high(WPSH)became another dominant moisture transport channel.The moisture in the lower troposphere was mainly associated with the southeasterly moisture transport from the South China Sea and the East China Sea,and the moisture in the middle troposphere was mainly transported from the BOB and Indian Ocean.The control experiment well reproduced the distribution and intensity of rainfall and moisture transport.By comparing the control and three sensitivity experiments,we found that the moisture transported from Typhoon Vicente and a tropical cyclone in the BOB both significantly affected this extreme rainfall event.After Typhoon Vicente was removed in a sensitivity experiment,the maximum 24-h accumulated rainfall in north China was reduced by approximately 50%compared with that of the control experiment,while the rainfall after removing the tropical cyclone was reduced by 30%.When both the typhoon and tropical cyclone were removed,the southwesterly moisture transport was enhanced.Moreover,the sensitivity experiment of removing Typhoon Vicente also weakened the tropical cyclone in the BOB.Thus,the moisture pump driven by Typhoon Vicente played an important role in maintaining and strengthening the tropical cyclone in the BOB through its westerly airflow.Typhoon Vicente was not only the moisture transfer source for the southwesterly monsoonal moisture but also affected the tropical cyclone in the BOB,which was a key supply source of long-distance moisture transport for the extreme rainfall event on July21,2012 in Beijing.
基金supported by the National MCF Energy R&D Program of China(No.2018YFE0303100)National Natural Science Foundation of China(No.11975038)。
文摘The inward particle transport is associated with the formation of peaked density profiles,which contributes to improve the fusion rate and the realization of steady-state discharge.The active control of inward particle transport is considered as one of the most critical issues of magnetic confinement fusion.Recently,it is realized preliminarily by adding a biased endplate in the Peking University Plasma Test(PPT)device.The results reveal that the inward particle flux increases with the bias voltage of the endplate.It is also found that the profile of radial electric field(Er)shear is flattened by the increased bias voltage.Radial velocity fluctuations affect the inward particle more than density fluctuations,and the frequency of the dominant mode driving inward particle flux increases with the biased voltage applied to the endplate.The experimental results in the PPT device provide a method to actively control the inward particle flux using a biased endplate and enrich the understanding of the relationship between E_(r)×B shear and turbulence transport.
基金supported by National Natural Science Foundation of China(No.12135015)the Users with Excellence Program of Hefei Science Center,CAS(No.2021HSCUE012)+3 种基金the National Key R&D Program of China(No.2022Y FE03010003)the Major Science and Technology Infrastructure Maintenance and Reconstruction Projects of the Chinese Academy of Sciences 2021the Special Funds for Improving Conditions for Scientific Research in National Scientific Institutions 2022the China Scholarship Council。
文摘At the EAST tokamak, the ion temperature(T_(i)) is observed to be clamped around 1.25 keV in electron cyclotron resonance(ECR)-heated plasmas, even at core electron temperatures up to 10 keV(depending on the ECR heating power and the plasma density). This clamping results from the lack of direct ion heating and high levels of turbulence-driven transport. Turbulent transport analysis shows that trapped electron mode and electron temperature gradient-driven modes are the most unstable modes in the core of ECR-heated H-mode plasmas. Nevertheless, recently it was found that the T_(i)/T_(e)ratio can increase further with the fraction of the neutral beam injection(NBI) power, which leads to a higher core ion temperature(Ti0). In NBI heating-dominant H-mode plasmas, the ion temperature gradient-driven modes become the most unstable modes.Furthermore, a strong and broad internal transport barrier(ITB) can form at the plasma core in high-power NBI-heated H-mode plasmas when the T_(i)/T_(e)ratio approaches ~1, which results in steep core Teand Tiprofiles, as well as a peaked neprofile. Power balance analysis shows a weaker Teprofile stiffness after the formation of ITBs in the core plasma region, where Ticlamping is broken,and the core Tican increase further above 2 keV, which is 80% higher than the value of Ticlamping in ECR-heated plasmas. This finding proposes a possible solution to the problem of Ticlamping on EAST and demonstrates an advanced operational regime with the formation of a strong and broad ITB for future fusion plasmas dominated by electron heating.
基金supported by the National Natural Science Foundation of China(51973157,51873152)Project funded by the China Postdoctoral Science Foundation(2022M711959)State Key Laboratory of Membrane and Membrane Separation,Tiangong University。
文摘With the depletion of fossil fuels and the demand for high-performance energy storage devices,solidstate lithium metal batteries have received widespread attention due to their high energy density and safety advantages.Among them,the earliest developed organic solid-state polymer electrolyte has a promising future due to its advantages such as good mechanical flexibility,but its poor ion transport performance dramatically limits its performance improvement.Therefore,single-ion conducting polymer electrolytes(SICPEs)with high lithium-ion transport number,capable of improving the concentration polarization and inhibiting the growth of lithium dendrites,have been proposed,which provide a new direction for the further development of high-performance organic polymer electrolytes.In view of this,lithium ions transport mechanisms and design principles in SICPEs are summarized and discussed in this paper.The modification principles currently used can be categorized into the following three types:enhancement of lithium salt anion-polymer interactions,weakening of lithium salt anion-cation interactions,and modulation of lithium ion-polymer interactions.In addition,the advances in single-ion conductors of conventional and novel polymer electrolytes are summarized,and several typical highperformance single-ion conductors are enumerated and analyzed in what way they improve ionic conductivity,lithium ions mobility,and the ability to inhibit lithium dendrites.Finally,the advantages and design methodology of SICPEs are summarized again and the future directions are outlined.
基金supported by the National Natural Science Foundation of China,Nos.32371070 (to JT),31761163005 (to JT),32100824 (to QX)the Shenzhen Science and Technology Program,Nos.RCBS20210609104606024 (to QX),JCY20210324101813035 (to DL)+4 种基金the Guangdong Provincial Key S&T Program,No.2018B030336001 (to JT)the Key Basic Research Program of Shenzhen Science and Technology Innovation Commission,Nos.JCYJ20200109115405930 (to JT),JCYJ20220818101615033 (to DL),JCYJ20210324115811031 (to QX),JCYJ20200109150717745 (to QX)Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological Diseases,No.ZDSYS20220304163558001 (to JT)Guangdong Provincial Key Laboratory of Brain Connectome and Behavior,No.2023B1212060055 (to JT)the China Postdoctoral Science Foundation,No.2021M693298 (to QX)。
文摘The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with emotions.Specifically, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal mice.After knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was obviously mitigated. Additionally, administration of an excitatory amino acid transporter 2 inhibitor in the basolateral amygdala yielded a notable reduction in anxiety level among mice subjected to stress. These results suggest that basolateral amygdala astrocytic excitatory amino acid transporter 2 plays a role in in the regulation of unpredictable chronic mild stress-induced anxiety-like behavior by impacting the activity of local glutamatergic neurons, and targeting excitatory amino acid transporter 2 in the basolateral amygdala holds therapeutic promise for addressing anxiety disorders.
基金Project supported by the National Key Research and Development Program of China(Grant No.2017YFE0301203)the Innovation Program of Southwestern Institute of Physics(Grant No.202301XWCX001)+2 种基金the Sichuan Science and Technology Program(Grant Nos.2023ZYD0014 and 2021YFSY0044)the National Natural Science Foundation of China(Grant No.12175055)the Shenzhen Municipal Collaborative Innovation Technology Program-International Science and Technology Cooperation Project(Grant No.GJHZ20220913142609017)。
文摘The dynamics of long-wavelength(kθ<1.4 cm^(-1)),broadband(20 kHz–200 kHz)electron temperature fluctuations(Te/Te)of plasmas in gas-puff experiments are observed for the first time in HL-2A tokamak.In a relatively low density(ne(0)■0.91×10^(19)m^(-3)–1.20×10^(19)m^(-3))scenario,after gas-puffing the core temperature increases and the edge temperature drops.On the contrary,temperature fluctuation drops at the core and increases at the edge.Analyses show the non-local emergence is accompanied with a long radial coherent length of turbulent fluctuations.While in a higher density(ne(0)?1.83×10^(19)m^(-3)–2.02×10^(19)m^(-3))scenario,the phenomena are not observed.Furthermore,compelling evidence indicates that E×B shear serves as a substantial contributor to this extensive radial interaction.This finding offers a direct explanatory link to the intriguing core-heating phenomenon witnessed within the realm of non-local transport.
基金Project supported by the National Natural Science Foundation of China (Grant No.12104012)the Natural Science Foundation of Anhui Province of China (Grant No.2108085MB42)+2 种基金the Key Research and Development Plan of Anhui Province of China (Grant No.202104a05020048)the Major Science and Technology Project of Anhui Province of China (Grant No.2021e03020007)the Doctor Foundation of Anhui Jianzhu University (Grant No.2019QDZ45)。
文摘Bismuthene,an inherently hexagonal structure characterized by a huge bulk gap,offers a versatile platform for investigating the electronic transport of various topological quantum states.Using nonequilibrium Green's function method and Landauer-Büttiker formula,we thoroughly investigate the transport properties of various Hall-type quantum states,including quantum spin Hall(QSH)edge states,quantum valley Hall kink(QVHK)states,and quantum spin-valley Hall kink(QSVHK)states,in the presence of various disorders.Based on the exotic transport features,a spin-valley filter,capable of generating a highly spin-and valley-polarized current,is proposed.The valley index and the spin index of the filtered QSVHK state are determined by the staggered potential and the intrinsic spin-orbit coupling,respectively.The efficiency of the spin-valley filter is supported by the spacial current distribution,the valley-resolved conductance,and the spin-resolved conductance.Compared with a sandwich structure for QSVHK,our proposed spin-valley filter can work with a much smaller size and is more accessible in the experiment.
文摘The wadi dahab delta is in a dry, arid coastal zone within Egypt’s south Sinai Peninsula’s eastern portion. The primary water source is the Quaternary coastal alluvial aquifer. The groundwater salinity varies from 890to 8213 mg/L, with a mean value of 3417 mg/L. The dissolved major ions have been used to calculate the seawater mixing index(SWMI) using a linear equation that discriminates the groundwater mostly affected by water–rock interaction(SWMI 1>) and other samples mixed with Seawater(SWMI < 1). The isotopic composition of groundwater for specifically chosen groundwater samples ranges from-0.645‰ to +5.212‰ for δ^(18)O and from-9.582‰ to + 22.778‰ for δ^(2)H, where the seawater represented by a Red Sea water sample(δ^(18)O + 1.64‰-δ^(2)H + 9.80‰) and reject brine water are considerably enriched the isotopic groundwater values. The geochemical NETPATH model constrained by the dissolved significant ions, isotopes, and the rock aquifer forming minerals as phases indicate the mixing percent with the seawater ranges from 9% to 97% of seawater from 91% to 3% of original recharge water. According to the SEAWAT 3-D flow models, seawater has penetrated the Northeastern Dahab delta aquifer, with the intrusion zone extending1500 m inland. The salt dissolution, upwelling of saline water, recharge from the upstream mountain block, and seawater encroachment are the primary aspects contributing to the deterioration of groundwater quality. These findings may have significance for effective groundwater withdrawal management in arid locations worldwide with similar hydrogeological systems.
基金supported by the Natural Science Foundation of Jiangsu Higher Education Institutions of China(23KJA210003)the Open Project Program of Joint International Research Laboratory of Agriculture and Agri-Product Safety,the Ministry of Education of China,Yangzhou University(JILAR-KF202202).
文摘Variations in the nutrients and water that plants require for metabolism,development,and the maintenance of cellular homeostasis are the main causes of abiotic stress in plants.It has,however,hardly ever been studied how these transporter proteins,such as aquaporin which is responsible for food and water intake in cell plasma mem-branes,interact with one another.This review aims to explore the interactions between nutrient transporters and aquaporins during water and nutrient uptake.It also investigates how symbiotic relationships influence the plant genome’s responses to regulatory processes such as photoperiodism,senescence,and nitrogenfixation.These responses are observed in reaction to various abiotic stresses.For instance,plasma membrane transporters are upregulated during macronutrient insufficiency,tonoplast transporters are overexpressed,and aquaporins are downregulated in micronutrient deficiency.Additionally,tolerant plants often exhibit increased expression of nutrient transporters and aquaporins in response to drought,salt,and cold temperatures.To better comprehend plant stress tolerance to abiotic challenges including starvation,K famine,salt,and freezing temperatures,both classes of nutrient and water transporters should be considered at the same time.
基金Project supported by the Doctoral Fund of Yanshan University (Grant No.B919)the Program of Independent Research for Young Teachers of Yanshan University (Grant No.020000534)the S&T Program of Hebei Province of China (Grant No.QN2016123)。
文摘Controlling mass transportation using intrinsic mechanisms is a challenging topic in nanotechnology.Herein,we employ molecular dynamics simulations to investigate the mass transport inside carbon nanotubes(CNT)with temperature gradients,specifically the effects of adding a static carbon hoop to the outside of a CNT on the transport of a nanomotor inside the CNT.We reveal that the underlying mechanism is the uneven potential energy created by the hoops,i.e.,the hoop outside the CNT forms potential energy barriers or wells that affect mass transport inside the CNT.This fundamental control of directional mass transportation may lead to promising routes for nanoscale actuation and energy conversion.
基金Project supported by the National Key R&D Program of China(Grant No.2022YFA1403603)the National Natural Science Foundation of China(Grant Nos.U2032213,12104461,12374129,and 12304156)+1 种基金Chinese Academy of Sciences(Grant Nos.YSBR-084,and JZHKYPT-2021-08)A portion of this work was supported by the High Magnetic Field Laboratory of Anhui Province.
文摘Due to the lack of inversion,mirror or other roto-inversion symmetries,chiral crystals possess a well-defined handedness which,when combined with time-reversal symmetry breaking from the application of magnetic fields,can give rise to directional dichroism of the electrical transport phenomena via the magnetochiral anisotropy.In this study,we investigate the nonreciprocal magneto-transport in microdevices of NbGe_(2),a superconductor with structural chirality.A giant nonreciprocal signal from vortex motions is observed during the superconducting transition,with the ratio of nonreciprocal resistance to the normal resistanceγreaching 6×10^(5)T^(-1)·A^(-1).Interestingly,the intensity can be adjusted and even sign-reversed by varying the current,the temperature,and the crystalline orientation.Our findings illustrate intricate vortex dynamics and offer ways of manipulation on the rectification effect in superconductors with structural chirality.
基金supported by the the“JBGS”Project of Seed Industry Revitalization in Jiangsu Province,China(JBGS[2021]018)the Jiangsu Agricultural Innovation of New Cultivars,China(PZCZ201720)the Open Project Program of Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,China(K2020030)。
文摘Leaf color mutants in higher plants are considered to be ideal materials for studying the chlorophyll biosynthesis,photosynthesis mechanism and chloroplast development.Herein,we identified a spontaneous mutant,yc412,in cultivated cucumber that exhibited yellow cotyledons.The yellow-lethal mutant was diagnosed with an abnormal chloroplast ultrastructure,and reduced photosynthetic capacity and pigment content.Through bulked segregant analysis-based whole-genome sequencing and fine genetic mapping,we narrowed the yellow cotyledons (yc) locus to a 96.8 kb interval on chromosome 3.By resequencing and molecular cloning,we showed that Csyc is a potential candidate gene,which encodes a yellow stripe-like (YSL) transporter.The T to C mutation in the promoter region of Csyc caused the yellow cotyledon phenotype in yc412.Compared to YZU027A (WT),the expression of Csyc was significantly downregulated in the cotyledons of yc412.Silencing of Csyc in cucumber via virus-induced gene silencing resulted in chlorotic leaves,mainly by suppressing the chlorophyll content.Furthermore,a comparative transcriptome analysis revealed that chloroplast-related genes and chlorophyll biosynthesis genes were significantly downregulated in yc412 cotyledons.Our results provide new insights into the molecular function of the YSL transporter in plant chloroplast development and chlorophyll synthesis.
基金financially supported by the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City(Grant No.520LH052)the National Natural Science Foundation of China(Grant No.51909164).
文摘Deepsea mining has been proposed since the 1960s to alleviate the lack of resources on land.Vertical hydraulic transport of collected ores from the seabed to the sea surface is considered the most promising method for industrial applications.In the present study,an indoor model test of the vertical hydraulic transport of particles was conducted.A noncontact optical method has been proposed to measure the local characteristics of the particles inside a vertical pipe,including the local concentration and particle velocity.The hydraulic gradient of ore transport was evaluated with various particle size distributions,particle densities,feeding concentrations and mixture flow velocities.During transport,the local concentration is larger than the feeding concentration,whereas the particle velocity is less than the mixture velocity.The qualitative effects of the local concentration and local fluid velocity on the particle velocity and slip velocity were investigated.The local fluid velocity contributes significantly to particle velocity and slip velocity,whereas the effect of the local concentration is marginal.A higher feeding concentration and mixture flow velocity result in an increased hydraulic gradient.The effect of the particle size gradation is slight,whereas the particle density plays a crucial role in the transport.