A comprehensive analysis of the response of the fungal community structure to long-term continuous cropping in three typical upland crops

Certain agricultural management practices are known to affect the soil microbial community structure;however,knowledge of the response of the fungal community structure to the long-term continuous cropping and rotation of soybean,maize and wheat in the same agroecosystem is limited.We assessed the fungal abundance,composition and diversity among soybean rotation,maize rotation and wheat rotation systems and among long-term continuous cropping systems of soybean,maize and wheat as the effect of crop types on fungal community structure.We compared these fungal parameters of same crop between long-term crop rotation and continuous cropping systems as the effect of cropping systems on fungal community structure.The fungal abundance and composition were measured by quantitative real-time PCR and Illumina MiSeq sequencing.The results revealed that long-term continuous soybean cropping increased the soil fungal abundance compared with soybean rotation,and the fungal abundance was decreased in long-term continuous maize cropping compared with maize rotation.The long-term continuous soybean cropping also exhibited increased soil fungal diversity.The variation in the fungal community structure among the three crops was greater than that between long-term continuous cropping and rotation cropping.Mortierella,Guehomyces and Alternaria were the most important contributors to the dissimilarity of the fungal communities between the continuous cropping and rotation cropping of soybean,maize and wheat.There were 11 potential pathogen and 11 potential biocontrol fungi identified,and the relative abundance of most of the potential pathogenic fungi increased during the long-term continuous cropping of all three crops.The relative abundance of most biocontrol fungi increased in long-term continuous soybean cropping but decreased in long-term continuous maize and wheat cropping.Our results indicate that the response of the soil fungal community structure to long-term continuous cropping varies based upon crop types.

Effect of Different Cultivation Practices on Soil Physical and Chemical Properties for Greenhouse Vegetables under Long-Term Continuous Cropping

Five kinds of greenhouse vegetables( eggplant,loofah,tomato,cucumber and pepper) were selected in summer uprooting stage from greenhouse in Shouguang area,Shandong Province. Total nitrogen,ammonium nitrogen,nitrate nitrogen,available phosphorus,available potassium,organic matter content and p H,EC value of three soil layers were measured,respectively. The results showed that the total nitrogen,ammonium nitrogen,nitrate nitrogen,available phosphorus and available potassium in the soil were mainly accumulated in the upper soil,which made the soil acidification trend,because different farmers adopted different cultivation practices for different greenhouse vegetables in Shouguang region,but there was no significant effect on soil EC value. The input of ammonium nitrogen and nitrate nitrogen and other related fertilizers to greenhouse cucumber was higher than that of other greenhouse vegetables,but the amount of available potassium fertilizer and organic matter input to greenhouse tomato under different cultivation practices was lower than other greenhouse vegetables. In Shouguang area,the input of organic matter into greenhouse vegetables should be increased by increasing the proportion of manure input to increase the content of soil organic carbon,so as to achieve the balance of carbon and nitrogen ratio,and to provide a scientific basis for the establishment of an evaluation system for the environmental benefits brought about by chemical fertilizer reduction in greenhouse vegetables in Shouguang area.

Effects of Continuous Cropping on Soil Microbial Flora and Research Progress of Continuous Cropping Obstacle Reduction Techniques

Continuous cropping can bring economic benefits in a short time and meet the growing demand of agricultural products such as grain,but long-term continuous cropping will accelerate soil degradation,lead to the reduction of crop yield and the increase of disease rate,and destroy the balance of soil microbial structure.Therefore,it is not conducive to the sustainable development of soil ecosystem.In this paper,the problems caused by continuous cropping,such as imbalance of soil microbial flora,decrease of biodiversity,accumulation of root exudates and their effects on soil fertility and crop growth,were summarized,and some measures were suggested to alleviate the obstacles of continuous cropping,such as reasonable rotation,adjustment of intercropping planting mode and application of biological fertilizers.Moreover,the paper also looked forward to the development trend of continuous cropping obstacle reduction techniques,including the integration and application of biological techniques,the promotion of green ecological techniques and the application of intelligent management system.This study provides theoretical basis and technical support for the research of continuous cropping obstacle reduction techniques and promote the healthy and sustainable development of modern agriculture.

Impact of Continuous Cropping on Soil Phenolic Acid Substances and Research Progress on Continuous Cropping Obstacle Reduction Techniques

At present,long-term continuous cropping in agricultural production has formed a relatively common development trend.With the increase of continuous cropping years,soil phenolic acids are also affected to varying degrees.This paper summarized the effects of continuous cropping on soil phenolic acids and the research progress of continuous cropping obstacle reduction techniques,aiming at providing theoretical basis and technical support for the research of continuous cropping obstacle reduction techniques and promoting the healthy and sustainable development of modern agriculture.

Research Progress on Effects of Continuous Cropping on Soil Microbial Florae and Its Restoration

Continuous cropping has become a common form of agricultural production at present, but with the increase of continuous cropping years, continuous cropping obstacles such as soil-borne diseases and plant growth potential decline are becoming more and more common. At present, the causes of continuous cropping obstacles and continuous cropping restoration have become a hot issue in agricultural research. This paper summarized the effects of continuous cropping obstacles on soil microbial community structure and main technical methods to repair continuous cropping obstacles, such as agricultural measure management, microbial balance adjustment and soil improvement, aiming to provide theoretical reference for protecting the sustainable utilization of soil ecosystem and ensuring the stability of crop production.

Effects of Allelochemicals on Root Growth and Pod Yield in Response to Continuous Cropping Obstacle of Peanut

Continuous cropping(CC)obstacle is a major threat in legume crops production;however,the underlying mechanisms concerning the roles allelochemicals play in CC obstacle are poorly understood.The current 2-year study was conducted to investigate the effects of different kinds and concentrations of allelochemicals,p-hydroxybenzoic acid(H),cinnamic acid(C),phthalic acid(P),and their mixtures(M)on peanut root growth and productivity in response to CC obstacle.Treatment with H,C,P,and M significantly decreased the plant height,dry weight of the leaves and stems,number of branches,and length of the lateral stem compared with control.Exogenous application of H,C,P,and M inhibited the peanut root growth as indicated by the decreased root morphological characters.The allelochemicals also induced the cell membrane oxidation even though the antioxidant enzymes activities were significantly increased in peanut roots.Meanwhile,treatment with H,C,P,and M reduced the contents of total soluble sugar and total soluble protein.Analysis of ATPase activity,nitrate reductase activity,and root system activity revealed that the inhibition effects of allelochemicals on peanut roots might be due to the decrease in activities of ATPase and NR,and the inhibition of root system.Consequently,allelochemicals significantly decreased the pod yield of peanut compared with control.Our results demonstrate that allelochemicals play a dominant role in CC obstacle-induced peanut growth inhibition and yield reduction through damaging the root antioxidant system,unbalancing the osmolytes accumulation,and decreasing the activities of root-related enzymes.

Analysis of the Causes of Continuous Cropping Obstacles for Atractylodes macrocephala Koidz in Pingjiang County and Its Control Methods

First at all, it introduced the concept and the damages of continuous cropping obstacle. Then, it analyzed the causes of continuous cropping obstacles for Atractylodes macrocephala Koidz. In the end, in order to provide guidance for pro- moting sustainable development of Atractylodes macrocephala Koidz industry in Pingjiang County, it put forward some control methods for eliminating continuous cropping obstacles of Atractylodes macrocephala Koidz, including breeding varieties with high resistance; applying rotation cropping and intercropping reasonable; rational fertilization and soil disinfection; introducing antagonistic bacterial and eliminating au- tointoxication.

Investigation and Analysis of Continuous Cropping of Flue-cured Tobacco in Henan Province

The continuous cropping of flue-cured tobacco in the major producing areas of Henan Province by designing questionnaire, fieldwork, enquiry and phone calls, mails, and other forms, the data were obtained then classified and analyzed. The results showed that the main planting areas of flue-cured tobacco were in Nanyang, Xuchang, Luoyang, Sanmenxia, Pingdingshan, and Luohe where were the major producing areas of succession cropping of flue-cured tobacco with percentage of 20%-40% for three years and over three years, and a few places up to 50%. Morbidity was common with wide distribution, and the main diseases were black shank, viral disease, and black rot. It proved that tobacco was not suitable for continuous cropping. Both of tobacco disease and pest rate and mortality significantly increased by more than 40% under continuous cropping for three years and over three years, and the yield and quality of flue-cured tobacco also decreased after continuous cropping. For many places, the tobacco yield started to decrease in the second year of continuous cropping, and after three-year cropping, the yield decreased in the range of 9%-24%. Continuous cropping for a long time would make a serious decline in the quality of tobacco, resulting in non-economic benefits. Therefore, in order to reduce the occurrence of diseases and economic loss in tobacco production, it is recommended to avoid continuous cropping, or to adopt appropriate measures to control tobacco continuous cropping obstacles in the unavoidable case.

Effect of Continuous Application of Controlled Release Nitrogen Fertilizer in Various Types of Soil in Dong-Ting Lake Region under Double Rice Cropping System

[Objective] This study aimed to explore the effects of continuous application of controlled release nitrogen fertilizer under double rice cropping system. [Method] By modeling three types of paddy soils in Dong-Ting Lake area, four treatments as no fertilizer （CK）, urea, controlled release nitrogen fertilizer （CRNF） and 70% controlled release nitrogen fertilizer （70% CRNF） were designed in the micro-plot trials from 2005 to 2008. [Result] The rice yield in treatment CRNF at N 150 kg/hm2 was increased by 10.3%, 8.0% and 2.4% compared with treatment of urea, in alluvial sandy loamy paddy soil （ALS）, purple calcareous clayey paddy soil （PCS）, and reddish yellow loamy paddy soil （RYS）, respectively; and the yield in treatment of 70% CRNF was increased by 6.1%, 2.6% and -0.8%, respectively. The ranking order of nitrogen uptake amount by plant in early rice and late rice was CRNF 70% CRNF urea CK in all three types of soil. Nitrogen utilization efficiency of CRNF in above three types of soil was 60.7%, 59.6% and 56.3%, increased by 23.8%, 19.4% and 16.3% compared with that in treatment of urea, respectively. Nitrogen utilization efficiency of CRNF in early rice was increased year by year, and was higher than that of 70% CRNF during the whole experiment stage, while that in late rice was increased first and then decreased from the 3rd year. [Conclusion] Continuous application CRNF could alleviate the decreasing of soil nitrogen fertility and organic carbon especially in ALS, increase rice yield and nitrogen utilization efficiency in double-rice cropping system.

Arbuscular mycorrhizal fungi combined with exogenous calcium improves the growth of peanut(Arachis hypogaea L.)seedlings under continuous cropping

The growth and yield of peanut are negatively affected by continuous cropping.Arbuscular mycorrhizal fungi(AMF)and calcium ions(Ca^(2+))have been used to improve stress resistance in other plants,but little is known about their roles in peanut seedling growth under continuous cropping.This study investigated the possible roles of the AMF Glomus mosseae combined with exogenous Ca^(2+)in improving the physiological responses of peanut seedlings under continuous cropping.G.mosseae combined with exogenous Ca^(2+)can enhance plant biomass,Ca^(2+)level,and total chlorophyll content.Under exogenous Ca^(2+)application,the F_v/F_m in arbuscular mycorrhizal(AM)plant leaves was higher than that in the control plants when they were exposed to high irradiance levels.The peroxidase,superoxide dismutase,and catalase activities in AM plant leaves also reached their maximums,and accordingly,the malondialdehyde content was the lowest compared to other treatments.Additionally,root activity,and content of total phenolics and flavonoids were significantly increased in AM plant roots treated by Ca^(2+)compared to either G.mosseae inoculation or Ca^(2+)treatment alone.Transcription levels of AhCaM,AhCDPK,AhRAM1,and AhRAM2 were significantly improved in AM plant roots under exogenous Ca^(2+)treatment.This implied that exogenous Ca^(2+)might be involved in the regulation of G.mosseae colonization of peanut plants,and in turn,AM symbiosis might activate the Ca^(2+)signal transduction pathway.The combination of AMF and Ca^(2+)benefitted plant growth and development under continuous cropping,suggesting that it is a promising method to cope with the stress caused by continuous cropping.

Effects of Continuous Cropping of Panax notoginseng on the Properties of Rhizosphere Soil

[Objective] This experiment aimed to investigate the effects of continuous cropping of Panax notoginseng on the properties of rhizosphere soil. [Method] A total of 12 rhizospheres oil samples were collected in the fields continuously cropped with P. notoginseng for different years and the soil properties including pH value, contents of available N, available K, available P,total N, total K, total P and organic matter were determined. [Result] With the increase in the number of years of continuous cropping, seven soli indices: soil pH value, organic matter content, total N, total P, total K, available P and available K gradually increased, while available N showed a gradual downward trend. The contents of organic matter content, total N, total P, total K,available P, available K and available N after three years of continuous cropping were increased by 74.93%, 65.85%, 123.82%,18.78%, 341.67%, 120.16% and-32.16%, respectively, indicating that continuous cropping of P. notoginseng resulted in nutrient enrichment in rhizosphere soils. The pH value and available N in IBC(soil inside the border check) and UBC(soil under the border check) were higher than that in BBC(soil beside the border check), suggesting that the soil was gradually alkalized due to the continuous cropping of P. notoginseng. [Conclusion] These results suggest that pH change and nutrient imbalance may be the obstacles to the continuous cropping of P. notoginseng.

Effects of Tobacco-rice Continuous Cropping Years on Soil Physicochemical Properties and Tobacco Yield and Quality

To investigate the effects of long term tobacco-rice continuous cropping on soil nutrients and flue-cured tobacco leaf yield and quality, the soils of different con- tinuous cropping years were selected in tobacco-rice multiple system of Hengyang. Some important indexes, including pH, contents of organic matter and available nu- trients of N P K, contents of medium trace elements of soil samples, and contents of main chemical components of flue-cured tobacco samples were detected for fur- ther analyzing. The results showed that pH value of moderate continuous cropping soil（≤7 years） was more coordinated, in addition, leaf yield and quality of flue-cured tobacco were contained, and the sugar-nicotine ratio and potassium-chlorine ratio were proper. However, under long-term continuous cropping （≥10 years） condition, the soil became acidification, and yield and output value of flue-cured tobacco de- clined. It was suggested that improving soil quality by planting green manure or keeping fallow after continuous cropping 7 years in tobacco-rice system.

Effects of Se on Reducing Membrane Lipid Peroxidation of Soybean under Continuous Cropping Stress

Reports the effects of selenium on lipid peroxidation of soybean under continuous cropping stress.Results of pot culture and plot experiment showed that appropriate amount of Se addition increased the activity of glutathione peroxidase (GSH-Px) significantly,and cleared away the excessive free radicals existing in soybean plant under continuous cropping stress,decreased the concentration of malonaldehyde(MDA),which is the end product of lipid peroxidation,maintained the perfection of chloroplast and mitochondrion structure,enhanced the resistance of continuous cropped soybean,and therefore increased the dry weight of seedlong and yield.

Effects and Mechanisms of Oil Cakes on the Growth of Cucumber (Cucumis sativus L.) Under Continuous Cropping System

Fxperiments on the effect of soil amendment with rape (Brassica chinensis L.) seed cake, cotton (Gossypium hirsutum L.) seed or sesame (Sesamum indicum L.) seed cake on the growth of cucumber seedlings under a continuous cropping system were conducted in a greenhouse environment. The results indicated that two applications of sesame seed cake (0.1 and 0.5 %, w/w) increased the growth of cucumber, but the rape oil cake showed a negative effect at a rate of 1.5% (w/w). The sesame seed cake was separated into four fractions (Ⅰ, Ⅱ, Ⅲ and Ⅳ) according to the polarity, and all four fractions had a positive effect on the growth of cucumbers under a continuous cropping system. Fraction number Ⅲ was isolated into 25 proportions by silicon column, and only oleic acid, palmitic acid and octadecanoic acid were identified in proportion 10 by GC-MS in which 93.3% was oleic acid and palmitic acid. The oleic acid had a significant and positive effect on cucumber growth under salt stress at the level 30 mmol and showed slight resistance to several pathogenic fungi.

Changes in Soil Enzyme Activity and Nutrient Content in Different Years of Continuous Cropping Tobacco Fields

In order to find out changes in the main fertility indicators of continuous cropping tobacco fields in Henan Province with years of continuous cropping,5 typical tobacco fields( Jia County,Shaoling District,Fangcheng County,Xiangcheng County,and Song County) were studied. Indicators in question included activity of main enzymes( sucrase,urease,catalase,and acid phosphatase),p H value,organic matters,total nitrogen,available phosphorus and available potassium. The results indicate that in most tobacco fields,the activity of sucrase and urease increased in two years of continuous cropping,later,it showed a declining trend with increase in years of continuous cropping; in some fields,it directly declined with increase in years of continuous cropping; the activity of catalase increased in two years of continuous cropping,later,it showed a declining trend with increase in years of continuous cropping,and it became stable after three years of continuous cropping;generally,the activity of acid phosphatase declined with increase in the years of continuous cropping within 3 years continuous cropping,later,it became stable. Except Fangcheng County,the soil p H value of other districts( counties) generally declined with increase in years of continuous cropping,and it was in the range of 5. 6-6. 8. With the increase in continuous cropping,the soil organic matters( SOM) in tobacco fields of Jia County and Song County declined,changes of SOM in tobacco fields of Fangcheng County and Xiangcheng County were not obvious,and SOM showed the trend of first decline then rise in Shaoling District. In most tobacco fields( Jia County,Xiangcheng County,and Song County),the total nitrogen showed a declining trend; in Shaoling District,it showed a trend of first decline then rise; in Fangcheng County,it showed a rising trend. The available phosphorus firstly slightly declined then rose in tobacco fields of Shaoling District and Song County; it showed a trend of first rise then decline in Jia County; there were no obvious changes in Fangcheng County and Xiangcheng County. Changes in the available potassium were different in tobacco fields. In sum,there are certain rules for changes in soil enzyme activity and nutrient content in tobacco fields in Henan Province,but changes are not completely the same. It is concluded that the site conditions and farming activities exert a certain influence on soil enzyme activity,p H value,organic matters,and nitrogen,phosphorus and potassium content. Therefore,to solve obstacles in continuous cropping of tobacco fields,different regions should take different measures.

Study on Obstacles to Continuous Cropping of Vegetables and Soil Remediation Technology

Firstly,this paper analyzes the cause of obstacles to continuous cropping of vegetables,and then introduces the soil ecological remediation technology used for overcoming obstacles to continuous cropping of vegetables. Finally,this paper analyzes the effect of applying soil ecological remediation technology in overcoming obstacles to continuous cropping of vegetables.

Soil Fungal Diversity and Community Composition in Response to Continuous Sweet Potato Cropping Practices

Soil fungi are extremely important for maintaining soil health and plant production in agricultural systems.Currently,the effect of continuous cropping of sweet potato on soil fungal communities and physiochemical parameters has not been well documented.In the present study,four sweet potato fields consecutively monocultured for 1,2,3,and 4 years were selected to investigate the effect of monoculture on soil fungal communities through Illumina MiSeq sequencing.Continuous cropping of sweet potatoes dramatically altered the fungal community composition,whereas fungal diversity was almost unchanged.Ascomycota and Basidiomycota were the most abundant phyla in all soil samples,accounting for 32.59%and 21.14%of the average relative abundance,respectively.The abundance of some potential pathogens,such as Ascobolus spp,specifically Ascobolus stercorarius,and some unknown fungi increased significantly as the sweet potato monoculture period increased,and their presence were highly positively correlated with disease incidence.In contrast,Basidiomycota,Bullera,Fusarium and Trichocladium most likely play roles as antagonists of sweet potato disease development,as their relative abundance decreased significantly over time and were negatively correlated with disease incidence.Redundancy and correlation analyses revealed that soil pH and organic carbon content were the most important factors driving these changes.Our findings provided a dynamic overview of the fungal community and presented a clear scope for screening beneficial fungi and pathogens of sweet potato.

Effects of Crop Stubble on Physicochemical Properties of Continuous Cropping Soil and Cucumber Yield and Quality

By a pot experiment, two kinds of crop stubble (wheat, soybean) were added into continuous cropping soil of cucumber according to different quantity (0.5%, 1% and 2%), the effects of different kinds of stubble and quantity on the continuous cropping soil and growth of cucumber were investigated. The results showed that two kinds of crop stubble significantly decreased soil bulk density, and increased total porosity of soil. Each of all treatments significantly decreased the accumulation of salinity in soil within the entire growth period of cucumber. 2% wheat stubble treatment had the biggest drop in soil EC in the later period of cucumber growth. Soybean stubble treatment had the lesser effect on soil EC. Wheat and soybean stubble promoted the growth of cucumber, increased the yield of cucumber, and improved the quality of cucumber. 2% of wheat stubble treatment had a biggest increase in cucumber yield, which increased the yield by 34.23% compared with the control.

Screening and Verification of Genes Specifically Responding to Continuous Cropping Obstacle in Rehmannia glutinosa L.

Rehmannia glutinosa L.is one of the important medicinal crops in China.Continuous cropping obstacle severely restricts the yield and quality of R.glutinosa,but its molecular mechanism is still unclear.In this study,with widely-planted "Wen 85-5" as an experiment material,based on the digital gene expression profiling (DGE) data of previous five stress treatments (continuous cropping,phenolic acid,salt,drought and waterlogging) and the first cropping and continuous cropping treatments of R.glutinosa in five different periods (seedling period,elongation period,early expanding period,middle expanding period and later expanding period),80 candidate genes (|log 2 ratio|≥1,FDR <0.001) specifically responding to continuous cropping obstacle in R.glutinosa were screened.Functional analysis revealed that the differentially expressed genes were involved in the secretion and endocytosis of root cells,which may suggest that the recognition and absorption of allelopathic autotoxins by the roots of R.glutinosa is an important factor that restricts the development of roots in continuous cropping of R.glutinosa.In order to accurately lock genes specifically responding to continuous cropping obstacle in R.glutinosa,continuous cropping soil extract and ferulic acid and p-hydroxybenzonic acid were used to treat aseptic plantlets of R.glutinosa,respectively,and it was confirmed through qRT-PCR that the expression levels of some genes under phenolic acid treatment changed more severely than that under the continuous cropping soil extract treatment,and four key genes involved in the response of R.glutinosa to continuous cropping were finally locked.This study lays a foundation for further exploration of the molecular mechanism of continuous cropping obstacle.

Study on the Effect of "Crayfish-Rice Continuous Cropping" on Grain Production:A Case Study of Hubei Province

Taking Hubei Province as an example,starting with the present situation and existing problems of grain production,this paper expounds the great significance of"crayfish-rice continuous cropping"model in improving grain quality,and analyzes the beneficial influence of"crayfish-rice continuous cropping"on grain production and the difficulties it faces.This paper also puts forward some measures and suggestions for the further popularization of"crayfish-rice continuous cropping"in order to maintain food security.