Research Overview of Zhuang Medicine Fumigation Lotions

At present,there is little research on the application of Zhuang medicine fumigation lotions in skin diseases,and there is no research on related preparation processes and quality standards.In this paper,the characteristics of its formulation and the research progress in skin diseases are reviewed,in order to deepen the understanding of Zhuang medicine fumigation lotions and provide a more detailed theoretical basis for its clinical research,development and application.

Extraction Process of Zhuang Medicine Fumigation Lotion

[Objectives]To establish the extraction process and quality standard method of Zhuang medicine fumigation lotion.[Methods]The orthogonal design method was employed to optimize the water extraction process with the amount of water added,decocting time and extraction times as factors,and syringin content and dry extract yield as indexes.The content of syringin was determined by high performance liquid chromatography.[Results]The best water extraction process was:soaking in water for 1 h,decocting twice,added 10 times the amount of water each time,decocting for 1 h.The average content of syringin in 3 batches was 0.98 mg/g,and the average dry extract yield was 26.07%.[Conclusions]The project adopts water extraction method to prepare Zhuang medicine fumigation lotion,which has the characteristics of high efficiency and suitable for large-scale production.The quality control method is reliable,rapid and accurate,and can effectively control the quality of the lotion.

Quality Control of Zhuang Medicine Xiaoyan Zhiyang Lotion

[Objectives]To establish the TLC identification methods of Llicis Rotundae Cortex and Mahoniae Caulis and the determination method of syringin content in Zhuang Medicine Xiaoyan Zhiyang Lotion.[Methods]Thin layer chromatography(TLC)was used to qualitatively identify Llicis Rotundae Cortex and Mahoniae Caulis in Zhuang Medicine Xiaoyan Zhiyang Lotion.The quantitative analysis of syringin in Zhuang Medicine Xiaoyan Zhiyang Lotion was carried out by high-performance liquid chromatography(HPLC).Chromatographic conditions are as follows:Luna 5μm C_(18)((2)100 A(250 mm×4.6 mm,5μm)column was adopted;acetonitrile(10%)-water(90%)was as the mobile phase,and the flow rate was 1.0 mL/min;the detection wavelength was 265 nm;the sample size was 5μL;the column temperature was 30℃.[Results]Syringin had a good linear relationship with its peak area in the range of 0.190-2.856μg(R^(2)=0.9995).The RSD values of precision,stability and repeatability experiments were all lower than 2.5%,and the average sampling recovery rate(n=6)was 100.37%(RSD=-0.88%).[Conclusions]The method had the advantages of simple operation,strong specificity,good repeatability and stability,and can be used for the quality control of Zhuang Medicine Xiaoyan Zhiyang Lotion.

Clinical Observation of Chuanbai Antipruritic Lotion"Water Film"Wet Compress Combined with Chloramphenicol Prednisone Liniment in the Treatment of Acute Eczema

[Objectives]To observe and analyze the clinical efficacy and possible mechanism of Chuanbai antipruritic lotion"water film"wet compress combined with chloramphenicol prednisone liniment in the treatment of acute eczema.[Methods]A total of 76 acute eczema cases admitted to Shiyan Taihe Hospital from January 2022 to March 2023 were divided into Western medicine treatment group and integrated traditional Chinese and Western medicine group.In the Western medicine treatment group,chloramphenicol and prednisone liniment was applied to the skin lesions and oral administration of cetirizine hydrochloride.The course of treatment in both groups was 2 weeks(w).The levels of interferon-γ(IFN-γ),interleukin-4(IL-4)and the ratio of IFN-γ/IL-4 in the peripheral blood of patients before and 2 weeks after treatment,as well as serum immunoglobulin E(IgE),anti-IgE antibody and histamine(HA)level,and with skin lesions disappearing time,skin oil,transepidermal water loss(TEWL),eczema area and severity index(EASI)score,total effective rate,degree of pruritus and traditional Chinese medicine quality of life scale(EPQOLS)score to evaluate the efficacy.[Results]Compared with the Western medicine treatment group at 2 w,the disappearance time of skin lesions in the integrated traditional Chinese and Western medicine group was shortened,TEWL,itching degree and EASI score,serum IgE and HA,and peripheral blood IL-4 levels were all decreased(P<0.05).IFN-γand IFN-γ/IL-4 ratio,anti-IgE antibody,EPQOLS score and total effective rate were all increased(P<0.05),and the difference was statistically significant.[Conclusions]Chuanbai antipruritic lotion"water film"wet compress combined with chloramphenicol prednisone liniment is an optimized and safe and efficient method for the treatment of acute eczema,which can quickly relieve the symptoms of inflammatory damage of eczema and restore the skin barrier function,dry dampness and relieve itch.Inhibition of the release of histamine active substances and regulation of immunity may be the main mechanisms.

Therapeutic Evaluation of 2% Tea Lotion in Comparison with 5% Zinc Sulfate Solution in the Treatment of Acne Rosacea

Background: There are many effective topical and systemic therapies for acne rosacea. Topical zinc sulfate and tea lotion have been used in treatment of acne vulgaris. Objectives: To evaluate effectiveness of 2% tea lotion in comparison with 5% zinc sulfate solution in the treatment of acne rosacea. Patients and Methods: This single, blinded, comparative therapeutic clinical trial was carried out in the Department of Dermatology—Baghdad Teaching Hospital from June 2006-December 2007. Twenty two patients with acne rosacea were divided into two groups and were instructed to apply the following lotions for two months, Group A used 2% tea lotion twice daily while Group B used 5% zinc sulfate solution twice daily. Patient with severe acne rosacea were excluded from the study. Clinical improvement was evaluated every 2 weeks by determination of the disease severity score (Sharquie’s score) before and after treatment. Results: Eighteen patients with acne rosacea completed the study with their ages ranging from 26 - 70 (40.5 ± 10.9) years. For Group A and Group B were 39.7 ± 8.2, 41.2 ± 13.1 years respectively, with eight patients in Group A, ten patients in Group B. Five percent zinc sulfate solution was statistically significant in reducing the disease severity score in acne rosacea, while 2% tea lotion was beneficial but did not reach statistically significant level as zinc sulfate solution. Conclusion: Five percent zinc sulfate solution was a good alternative remedy to be used in patients with acnerosacea.

Study on Quality Standards of Children Antipyretic Lotion

[Objectives]To establish suitable quality standards of children antipyretic lotion.[Methods]Qualitative identification was performed for Paederia scandens and Artemisia carvifolia in Children Antipyretic Lotion by thin-layer chromatography(TLC),and the content of paederosidic acid in children antipyretic lotion was determined by high performance liquid chromatography(HPLC).Besides,chromatographic column Feinigen XPeonyx C18(250 mm×4.6 mm,5μm),mobile phase was used,gradient elution was carried out with acetonitrile-0.1%phosphoric acid aqueous solution,the detection wavelength was 236 nm,the flow rate was 1.0 mL/min,the column temperature was 30℃,and the injection volume was 10μL.[Results]The thin-layer chromatography of P.scandens and A.carvifolia reference materials showed clear spots,good separation effect,strong specificity,and no interference in the limit control;paederosidic acid showed a good linear relationship in the range of 0.65-9.75μg(R2=0.9996),the average sample recovery rate was 99.72%(RSD=2.52%).[Conclusions]The qualitative and quantitative methods established in this experiment are simple,reproducible,and the results are accurate and reliable,and can be applied as a quality control method for children antipyretic lotions.

Simple HPLC Analysis of Hinokitiol in Skin Lotion with Visible Light Detection after Pre-Column Dabsylation

Hinokitiolis frequently added to personal care products as an antibacterial agent. We previously established an HPLC-UV method for determination of hinokitiol in skin lotion after pre-column derivatization with 4-fluoro-7-nitro-2,1,3-benzoxadiazole. However, the labeling reagent is expensive, and derivatives of degradation products of parabens, which may be added to skin lotion as general preservatives, interfered with the peak of the hinokitiol derivative. In this study, the concentration of hinokitiol in skin lotions was determined by HPLC with a visible light detector (450 nm) after pre-column derivatization with 4-(dimethylamino)azobenzene-4’-sulfonyl chloride (Dabsyl-Cl), a more economical reagent. A standard curve was obtained after derivatization with Dabsyl-Cl in borate buffer (pH 9.5) at 55&deg;C for 10 min. The retention time of Dabsyl-hinokitiol was 6.8 min. The calibration plot was linear in the range of 1.25 to 40 μg/mL with a r2 value of 0.9991, and the lower limit of quantification and detection were 0.60 μg/mL (absolute amount of 0.86 ng/20μL injection, signal-to-noise ratio of 10:1) and 0.18 μg/mL (absolute amount of 0.26 ng/20μL injection, signal-to-noise ratio of 3:1), respectively. The coefficient of variation was less than 8.8%. Seven Dabsyl-paraben derivatives showed little interference with the peak of Dabsyl-hinokitiol. The developed system was used to determine the content of hinokitiol in two skin lotions. Addition-recovery tests gave satisfactory results.

Formulated citrus peel extract gold lotion improves cognitive and functional recovery from traumatic brain injury(TBI)in rats

Traumatic brain injury(TBI)is closely related to neuro-inflammatory response and causes a complex pathological process and serious consequence.In this study,we explored whether the formatted citrus peel extract gold lotion(GL)could have a therapeutic effect on rats suffered TBI.TBI rat model was prepared by the electric Cortical Contusion Impactor(eCCI)device.Prevention against TBI by GL was assessed by the behavioral,organizational of rats and the molecular markers in the brain injury area.The results showed that GL could reduce nerve damage and neurological dysfunction.The mechanisms probably due to that GL could not only reduce the associated damage markers including GFAP,OX-42,TNF-α,COX-2,NF-κB and TLR4 but also promote the expression of VEGF which is responsible for protecting neuronal cells in the injury area.These findings revealed that GL could be a promising preparation for TBI prevention.

Therapeutic Effect of Qianjin Lotion on Eczema in Mice and Its Mechanism

[Objectives]To study the therapeutic effect and mechanism of Qianjin Lotion on eczema in mice.[Methods]A mouse eczema model was established by the 2,4-dinitrochlorobenzene method.The indicators of mouse ear swelling degree,the number of dermal inflammatory cells,the number of body licking reactions,the IL-2 and TNF-αcontent in serum were detected to explore the therapeutic effect and mechanism of Qianjin Lotion on eczema in mice.[Results]Qianjin Lotion can effectively eliminate skin inflammation,reduce the degree of auricle swelling,reduce the number of dermal inflammation cells,reduce the number of body licking reactions,and reduce the IL-2 and TNF-αcontent in serum.[Conclusions]Qianjin Lotion can alleviate the symptoms of eczema in mice,reduce inflammatory factors.Combined with oral administration of Qianjin granules,it can treat eczema and achieve good effects.

Preparation and Performance Test of Gexianmi (Nostoc sphaeroides kützing) Moisturizing Lotion

Gexianmi moisturizing lotion was prepared from Gexianmi extract, carbomer, EDTA-Na2, citric acid, glycerin, butanediol, ceramide, cetearyl alcohol, hydrogenated polydecene, shea butter, etc., and skin moisture contents of different treatments were measured. The data were analyzed by variance analysis and multiple comparisons. The results showed that: Gexianmi moisturizing lotion had a better moisturizing effect, which was better than that of anthocyanin moisturizing lotion. Therefore, Gexianmi moisturizing lotion can be used to effectively maintain the moisture of the human skin stratum corneum.

Study on the Efficacy of Vitamin C Lotion on Skin: Permeable and Anti-Aging

Vitamin C (ascorbic acid) plays an important role in maintaining skin health, and topical vitamin C supplementation can counteract oxidative stress induced by UVA, due to excellent reducibility. To clarify the efficacy of vitamin C on the skin in the carrier of lotion, we studied its permeability, irritation and anti-aging effect in vitro and in vivo, using Franz cell system, cell model and clinical test. The permeability test showed that vitamin C with 10%, 15%, 20% and 25% mass ratio could effectively penetrate skin. The 20% of vitamin C lotion (VCL-20%) had the highest efficiency of transdermal penetration and the diffusion percentage reached 84.707% after 24 h. Besides, the permeation quantity of VCL-20% was 1.43 times that of the control group. Irritation test showed that the cytotoxicity of vitamin C lotion was low. And no allergic reaction happened in the occlusive patch test. Compared with the control group, using vitamin C lotion for 28 days could significantly improve subjects’ skin gloss of 10.53% and improve skin color, enhance facial skin elasticity and tightness of 9.20% and reduce wrinkle area of 12.27% (p < 0.05).

Exploring the mechanism of action of San Huang lotion for the topical treatment of eczema based on network pharmacology and molecular docking techniques

Objective:To predict the mechanism of action of San Huang lotion for topical treatment of eczema at the level of network pharmacology and molecular docking technology.Methods:The active ingredients and corresponding targets of San Huang lotion were collected using the TCMSP data platform and UniProt;the relevant gene targets of San Huang lotion were collected using the GeneCards database,OMIM database,TTD database,and DrugBank database;the intersection targets of San Huang lotion and eczema were obtained by creating avenn diagram;the key active ingredients and targets were screened by constructing a disease-active ingredient-target map,and the key active ingredients and core targets were screened out by constructing disease-active ingredient-target diagrams and PPInetwork diagrams;GOand KEGGenrichment analyses were conducted using the metascapewebsite and the higher significant entries were selected to produce bar charts and bubble plots.Finally,molecular docking of key active ingredients and core targets was performed.Results:A total of 134 active ingredients and 3,320 eczema-related target genes were screened for San Huang lotion,and 126 intersecting targets were obtained for San Huang lotion and eczema.The five key active ingredients of San Huang lotion for topical treatment of eczema and six core targets ofAKT1,TNF,IL6,IL1B,TP53andVEGFA were obtained by drug-active ingredient-target map and PPInetwork map.The GOand KEGGenrichment analysis showed that the topical treatment of eczema withSanHuanglotion might be mediated throughIL-17signaling pathway,TNFsignaling pathway and Th17cell differentiation pathway.The molecular docking results showed that the docking binding energy of key active ingredients and core targets was mostly less than-5 kcal/mol,and the docking was good.Conclusion:The quercetin,luteolin,wogonin,formononetin,beta-sitosterol and other active ingredients in San Huang lotion may act on eczema through AKT1,TNF,IL6,IL1B,TP53,VEGFA and other targets,and through IL-17signaling pathway,TNFsignaling pathway,Th17cell differentiation and other pathways.

健脾祛风汤联合皮炎洗剂治疗特应性皮炎疗效研究

目的:研究健脾祛风汤联合皮炎洗剂治疗特应性皮炎的效果及对免疫球蛋白E(IgE)和白介素-4(IL-4)的影响。方法:收集120例特应性皮炎患者临床资料,并开展回顾性对比研究。观察组60例,行健脾祛风汤联合皮炎洗剂;对照组60例,行皮炎洗剂干预。比较两组患者治疗效果,记录两组患者治疗前后皮肤屏障功能[皮脂含量、角质层含水量及经皮水分丢失(TEWL)含量]、血清因子(IL-4、IgE)水平及生活质量评分。结果:观察组患者整体疗效较对照组显著提高(P<0.05)。治疗后观察组患者皮脂含量和角质层含水量均高于对照组,TEWL低于对照组,差异有统计学意义(P<0.05)。治疗后两组患者血清IL-4和IgE较治疗前均显著降低,且观察组低于对照组,差异有统计学意义(P<0.05)。治疗后两组皮肤病生活质量指数(DLQI)和特应性皮炎积分(SCORAD)较治疗前均显著降低,且观察组低于对照组(P<0.05)。结论:健脾祛风汤联合皮炎洗剂用于特应性皮炎效果显著,有助于保护皮肤屏障功能,减轻临床症状,改善患者生活质量。

功劳木外洗液对非哺乳期乳腺炎溃后期患者的效果

目的 探究功劳木外洗液对非哺乳期乳腺炎(NMP)溃后期患者创面愈合、窦道深度及垂体泌乳素即催乳素(PRL)的影响。方法 选取NMP溃后期患者140例,分为观察组和对照组,每组70例。采用Image J软件计算患者治疗前后的创面面积并统计渗液量,采用银质探针测量治疗前后患者的窦道深度,采用化学电发光法检测治疗前后患者PRL水平;比较2组疗效、创面面积、渗液量、窦道深度、PRL水平的变化。结果 观察组治疗总有效率优于对照组,治疗后观察组的创面面积和渗液量均低于对照组,治疗后观察组的窦道深度低于对照组,治疗后2组PRL均降低,且观察组的PRL低于对照组,观察组复发率低于对照组,差异有统计学意义(P<0.05)。结论 功劳木外洗液可以有效改善NMP溃后期患者创面愈合情况、窦道深度及PRL水平。

妇安消疹洗液三联法治疗神经性皮炎的疗效

目的观察妇安消疹洗液三联法治疗神经性皮炎的疗效。方法选取2019年1月—2020年12月十堰市太和医院皮肤病中心收治的神经性皮炎患者166例,按照年龄排序以随机数字表法分为观察组和对照组,各83例。对照组在皮损处涂抹糠酸莫米松乳膏治疗,观察组在对照组基础上在皮损处涂抹妇安消疹洗液与白及多糖乳膏治疗,1周为1个疗程,2组均连续治疗3周。比较2组疗效,治疗前后血清组胺、白介素-5(IL-5)水平、湿疹面积及严重度指数(EASI)、皮肤病生活质量指数(DLQI),不良反应及随访3个月后复发率。结果观察组总有效率为89.16%,高于对照组的66.26%(χ^(2)=14.299,P=0.003)。治疗3周后,2组血清组胺、IL-5水平低于治疗前,且观察组低于对照组(P<0.01);2组EASI评分低于治疗前,DLQI评分高于治疗前,且观察组降低或升高幅度大于对照组(P<0.01)。2组治疗期间均无明显不良反应发生;随访3个月后,观察组复发率为4.82%(4/83),对照组复发率为8.43%(7/83),2组比较差异无统计学意义(χ^(2)=5.725,P=0.221)。结论妇安消疹洗液、白及多糖乳膏配合糠酸莫米松乳膏治疗神经性皮炎效果显著,可有效降低炎性反应,改善患者临床症状及生活质量,且不良反应少,复发率低。

鄂尔多斯盆地东缘深煤层用一体化变黏压裂液研究及应用

结合深煤层大规模压裂开发工艺要求,研发了一套一体化变黏压裂液体系,该压裂液体系由乳液稠化剂、多功能添加剂等组成,可通过调整乳液稠化剂的浓度实时调整为低黏-中黏-高黏滑溜水体系。实验测试表明,该压裂液体系在速溶、增黏、降阻、耐剪切性等方面表现优异:速溶时间20~30 s;在剪切速率为1 s^(-1)→1000 s^(-1)→1 s^(-1)下表观黏度基本不变;流动剪切下降阻率>70%且变化不大。此外,该体系具有较低的表面张力和较好的防膨性能,表现为对煤岩具有较小的岩心伤害率。配合体积改造压裂工艺,在在鄂尔多斯盆地深煤层压裂开发中得到规模化应用,增产效果良好。

可变粘压裂液研究及在深煤层中应用

针对深煤层压裂开发需要,研发一套可变粘压裂液体系,通过粘度、降阻率、表面张力、界面张力等指标优选构建了低粘-中粘-高粘滑溜水体系,并对体系滤失性能、破胶性能和岩心伤害性能进行了评价。实验结果表明:随着体系粘度增加,降阻率先增加后降低;高粘度有助于降低滤失。通过调节破胶剂用量实现破胶可控,对煤岩具有较小的岩心伤害率。配合体积改造压裂工艺,可有效助力深煤层开发。优选的可变粘压裂液具有较好的推广和应用前景。

下肢洗剂治疗膝骨关节炎临床观察

目的探讨下肢洗剂治疗膝骨关节炎的效果。方法选择60例膝骨关节炎患者作为研究对象,将其随机分成试验组和对照组,各30例。对照组给予双氯芬酸二乙胺乳胶剂外用,试验组给予下肢洗剂外用,2组均持续治疗2周。观察2组的临床疗效,比较2组治疗前后西安大略大学和麦克马斯特大学骨关节炎指数(WOMAC)量表评分、膝关节综合评分、血清学指标变化情况,以及不良反应发生情况。结果试验组的愈显率为66.67%(20/30),高于对照组的50.00%(15/30),差异有统计学意义(P<0.05)。治疗后,2组患者的WOMAC量表评分、膝关节综合评分及白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)水平均有不同程度下降,且试验组下降程度大于对照组,差异有统计学意义(P<0.05)。2组不良反应发生率比较,差异无统计学意义(P>0.05)。结论下肢洗剂对于阳虚寒凝型膝骨关节炎具有治疗作用,可以很好地改善其临床症状,使血清炎性因子水平降低,在临床有一定的应用推广价值。

硝酸咪康唑栓规范化治疗霉菌性阴道炎患者的临床效果观察

对霉菌性阴道炎患者进行硝酸咪康唑栓规范化治疗,分析其治疗效果。选取南皮县人民医院2021年5月—2022年5月份住院的80例霉菌性阴道炎患者为研究对象,采用随机数字法将其分为观察组和对照组,每组40例。其中,观察组应用洁阴康洗液联合硝酸咪康唑栓,而对照组单独应用洁阴康洗液治疗,观察两种治疗方法的疗效、复发率、不良反应、阴道pH值,清洁度等指标。同时对两组患者的外阴瘙痒症状、白带增多症状、阴道黏膜发红肿胀症状消失的时间进行比较。在治疗有效率方面,对照组75.00%,观察组的临床有效率92.50%,相比较观察组高于对照组(P<0.05);观察组的外阴瘙痒、白带增多和阴道黏膜红肿,以及阴道烧烫感的缓解时间比对照组要短(P<0.05);观察组患者的阴道pH值、整洁度改善效果更好,差异有统计学上的显著性(P<0.05);观察组的不良反应发生率明显高于对照组(P<0.05);观察组的复发率为5.00%,比对照组22.50%更低(P<0.05)。研究发现,对霉菌性阴道炎患者应用硝酸咪康唑栓规范化治疗可以有效缓解患者的临床症状,提高治疗有效率,值得推广。