Environmental conditions, including ambient temperature, play important roles in survival growth development, and reproduction of the Japanese sea cucumber, Apostichopus japonicus. Low temperatures result in slowed gr...Environmental conditions, including ambient temperature, play important roles in survival growth development, and reproduction of the Japanese sea cucumber, Apostichopus japonicus. Low temperatures result in slowed growth and skin ulceration disease. In a previous study, we investigated the effect of low temperature on gene expression profiles inA.japonicus by suppression subtractive hybridization （SSH）. Genes encoding Ferritin, Lysozyme, Hsp70, gp96, and AjToll were selected from a subtracted cDNA library of A. japonicus under acute cold stress. The transcriptional expression profiles of these genes were investigated in different tissues （coelomocyte, respiratory tree, intestine, longitudinal muscle） after exposure to acute and mild temperature dropping treatments. The results show that （1） the five cold-tolerance-related genes were found in all four tissues and the highest mRNA levels were observed in coelomocyte and respiratory tree; （2） under the temperature dropping treatments, three types of transcriptional regulation patterns were observed： primary suppression followed by up-regulation at -2℃, suppressed expression throughout the two treatments, and more rarely an initial stimulation followed by suppression; and （3） gene expression suppression was more severe under acute temperature dropping than under mild temperature dropping treatment. The five cold-tolerance-related genes that were ,distributed mainly in coelomocyte and respiratory tissues were generally down-regulated by low temperature stress but an inverse up-regulation event was found at the extreme temperature （-2℃）.展开更多
Evening primrose is a valuable oilseed crop with pharmaceutical and nutritional supplementation. Due to presence of valuable and rare gamma linolenic fatty acid, research on this plant has been started since 1970s by ...Evening primrose is a valuable oilseed crop with pharmaceutical and nutritional supplementation. Due to presence of valuable and rare gamma linolenic fatty acid, research on this plant has been started since 1970s by German and Britain scientists. Information about evening primrose cultivation is going to rise. Following to pervious studies the goal of this experiment was to clarify the effect of water and temperature stresses on seed yield and seed quality. Results showed that seed yield was significantly influenced by low temperature and water deficit. Plants grown under cold stress during rosette stage produced a lower seed yield than controls. The lowest seed yield was observed in the plants grown under permanent stress. Although the oil content of seeds was not influenced by low temperature stress, water stress strongly influenced the oil content and water stress stimulates oil accumulation. Like oil content, the GLA production seems to be increased under water stress conditions.展开更多
基金Supported by the National High Technology Research and Development Program of China(863 Program)(No.2012AA10A412)the National Natural Science Foundation of China(No.31072230)
文摘Environmental conditions, including ambient temperature, play important roles in survival growth development, and reproduction of the Japanese sea cucumber, Apostichopus japonicus. Low temperatures result in slowed growth and skin ulceration disease. In a previous study, we investigated the effect of low temperature on gene expression profiles inA.japonicus by suppression subtractive hybridization （SSH）. Genes encoding Ferritin, Lysozyme, Hsp70, gp96, and AjToll were selected from a subtracted cDNA library of A. japonicus under acute cold stress. The transcriptional expression profiles of these genes were investigated in different tissues （coelomocyte, respiratory tree, intestine, longitudinal muscle） after exposure to acute and mild temperature dropping treatments. The results show that （1） the five cold-tolerance-related genes were found in all four tissues and the highest mRNA levels were observed in coelomocyte and respiratory tree; （2） under the temperature dropping treatments, three types of transcriptional regulation patterns were observed： primary suppression followed by up-regulation at -2℃, suppressed expression throughout the two treatments, and more rarely an initial stimulation followed by suppression; and （3） gene expression suppression was more severe under acute temperature dropping than under mild temperature dropping treatment. The five cold-tolerance-related genes that were ,distributed mainly in coelomocyte and respiratory tissues were generally down-regulated by low temperature stress but an inverse up-regulation event was found at the extreme temperature （-2℃）.
文摘Evening primrose is a valuable oilseed crop with pharmaceutical and nutritional supplementation. Due to presence of valuable and rare gamma linolenic fatty acid, research on this plant has been started since 1970s by German and Britain scientists. Information about evening primrose cultivation is going to rise. Following to pervious studies the goal of this experiment was to clarify the effect of water and temperature stresses on seed yield and seed quality. Results showed that seed yield was significantly influenced by low temperature and water deficit. Plants grown under cold stress during rosette stage produced a lower seed yield than controls. The lowest seed yield was observed in the plants grown under permanent stress. Although the oil content of seeds was not influenced by low temperature stress, water stress strongly influenced the oil content and water stress stimulates oil accumulation. Like oil content, the GLA production seems to be increased under water stress conditions.
