Retrotransposon-like elements are major constituents of most eukaryotic genomes. For example, they account for roughly 90% of the wheat （Triticum aestivum） genome, Previous study on a wheat strain treated by low-ene...Retrotransposon-like elements are major constituents of most eukaryotic genomes. For example, they account for roughly 90% of the wheat （Triticum aestivum） genome, Previous study on a wheat strain treated by low-energy N^＋ ions indicated the variations in AFLP （Amplified Fragment Length Polymorphism ） markers, One such variation was caused by the re-activation of Tyl-copia-like retrotransposons, implying that the mutagenic effects of lowenergy ions might work through elevated activation of retrotransposons, In this paper an expression profile of Tyl-copia-like retrotransposons in wheat treated by low-energy N^＋ ions is reported, The reverse transcriptase （RT） domains of these retrotransposons were amplified by reverse-transcriptional polymerase chain reaction （RT-PCR） and sequentially cloned, 42 and 65 clones were obtained from the treated （CL） and control materials （CK）, respectively, Sequence analysis of each clone was performed by software. Phylogeny and classification were calculated responding to the sequences of the RT domains. All the results show that there is much difference in the RT domain between the control sample and the treated sample, Especially, the RT domains from the treated group encode significantly more functional ORF （open reading frames） than those from the control sample, This observation suggests that the treated sample has higher activation of retrotransposons, possibly as a consequence of low-energy ion beam irradiation, It also suggests that retrotransposons in the two groups impact the host gene expression in two different ways and carry out different functions in wheat cells.展开更多
The low-energy muon facility at PSI provides nearly fully polarized positive muons with tunable energies in the ke V range to carry out muon spin rotation(LE-μSR)experiments with nanometer depth resolution on thin fi...The low-energy muon facility at PSI provides nearly fully polarized positive muons with tunable energies in the ke V range to carry out muon spin rotation(LE-μSR)experiments with nanometer depth resolution on thin films,heterostructures, and near-surface regions. The low-energy muon beam is focused and transported to the sample by electrostatic lenses. In order to achieve a minimum beam spot size at the sample position and to enable the steering of the beam in the horizontal and vertical direction, a special electrostatic device has been implemented close to the sample position. It consists of a cylinder at ground potential followed by four conically shaped electrodes,which can be operated at different electric potential. In LE-μSR experiments, an electric field at the sample along the beam direction can be applied to accelerate/decelerate muons to different energies(0.5–30 keV). Additionally, a horizontal or vertical magnetic field can be superimposed for transverse or longitudinal field μSR experiments. The focusing properties of the conical lens in the presence of these additional electric and magnetic fields have been investigated and optimized by Geant4 simulations. Some experimental tests were also performed and show that the simulation well describes the experimental setup.展开更多
A screen for Arabidopsis fertility mutants, mutagenized by low-energy argon ion beam, yielded two partial male-sterile mutants tc243-1 and tc243-2 which have similar phenotypes. tc243-2 was investigated in detail. The...A screen for Arabidopsis fertility mutants, mutagenized by low-energy argon ion beam, yielded two partial male-sterile mutants tc243-1 and tc243-2 which have similar phenotypes. tc243-2 was investigated in detail. The segregation ratio of the mutant phenotypes in the M2 pools suggested that mutation behaved as single Mendelian recessive mutations, tc243 showed a series of mutant phenotypes, among which partial male-sterile was its striking mutant characteristic. Phenotype analysis indicates that there are four factors leading to male sterility, a. Floral organs normally develop inside the closed bud, but the anther filaments do not elongate sufficiently to position the locules above the stigma at anthesis, b. The anther locules do not dehisce at the time of flower opening （although limited dehiscence occurs later）, c. Pollens of mutant plants develop into several types of pollens at the trinucleated stage. as determined by staining with DAPI （4＇,6-diamidino-2-phenylindole）. which shows a variable size. shape and number of nucleus. d. The viability of pollens is lower than that of the wild type on the germination test in vivo and vitro.展开更多
Reconfigurable intelligent surface(RIS)is more likely to develop into extremely large-scale RIS(XL-RIS)to efficiently boost the system capacity for future 6 G communications.Beam training is an effective way to acquir...Reconfigurable intelligent surface(RIS)is more likely to develop into extremely large-scale RIS(XL-RIS)to efficiently boost the system capacity for future 6 G communications.Beam training is an effective way to acquire channel state information(CSI)for XL-RIS.Existing beam training schemes rely on the far-field codebook.However,due to the large aperture of XL-RIS,the scatters are more likely to be in the near-field region of XL-RIS.The far-field codebook mismatches the near-field channel model.Thus,the existing far-field beam training scheme will cause severe performance loss in the XL-RIS assisted nearfield communications.To solve this problem,we propose the efficient near-field beam training schemes by designing the near-field codebook to match the nearfield channel model.Specifically,we firstly design the near-field codebook by considering the near-field cascaded array steering vector of XL-RIS.Then,the optimal codeword for XL-RIS is obtained by the exhausted training procedure.To reduce the beam training overhead,we further design a hierarchical nearfield codebook and propose the corresponding hierarchical near-field beam training scheme,where different levels of sub-codebooks are searched in turn with reduced codebook size.Simulation results show the proposed near-field beam training schemes outperform the existing far-field beam training scheme.展开更多
This paper describes a real-time beam tuning method with an improved asynchronous advantage actor–critic(A3C)algorithm for accelerator systems.The operating parameters of devices are usually inconsistent with the pre...This paper describes a real-time beam tuning method with an improved asynchronous advantage actor–critic(A3C)algorithm for accelerator systems.The operating parameters of devices are usually inconsistent with the predictions of physical designs because of errors in mechanical matching and installation.Therefore,parameter optimization methods such as pointwise scanning,evolutionary algorithms(EAs),and robust conjugate direction search are widely used in beam tuning to compensate for this inconsistency.However,it is difficult for them to deal with a large number of discrete local optima.The A3C algorithm,which has been applied in the automated control field,provides an approach for improving multi-dimensional optimization.The A3C algorithm is introduced and improved for the real-time beam tuning code for accelerators.Experiments in which optimization is achieved by using pointwise scanning,the genetic algorithm(one kind of EAs),and the A3C-algorithm are conducted and compared to optimize the currents of four steering magnets and two solenoids in the low-energy beam transport section(LEBT)of the Xi’an Proton Application Facility.Optimal currents are determined when the highest transmission of a radio frequency quadrupole(RFQ)accelerator downstream of the LEBT is achieved.The optimal work points of the tuned accelerator were obtained with currents of 0 A,0 A,0 A,and 0.1 A,for the four steering magnets,and 107 A and 96 A for the two solenoids.Furthermore,the highest transmission of the RFQ was 91.2%.Meanwhile,the lower time required for the optimization with the A3C algorithm was successfully verified.Optimization with the A3C algorithm consumed 42%and 78%less time than pointwise scanning with random initialization and pre-trained initialization of weights,respectively.展开更多
The potential of SCAR marker for discrimination of a Thai jasmine rice (Oryza sativa L. cv. KDML 105) mutant, BKOS6, obtained from ion-beam-induced mutation, was evaluated. The improved rice variety, BKOS6, exhibited ...The potential of SCAR marker for discrimination of a Thai jasmine rice (Oryza sativa L. cv. KDML 105) mutant, BKOS6, obtained from ion-beam-induced mutation, was evaluated. The improved rice variety, BKOS6, exhibited many remarkable characteristics which fitted the multiple cropping system characteristics of progressive agriculture including photoperiod insensitivity, early flowering, short in stature, and purple pigment accumulation in pericarp. The BKOS6 rice grain extract has already been proved that it exhibited higher antioxidant properties than the KDML 105 and other tested rice grain extracts. In this study, the BKOS6 specific SCAR marker was developed by HAT-RAPD analysis of rice genomic DNA. The marker was successfully used to identify BKOS6 variety and its hybrid varieties containing purple pigment accumulation in plant tissues. Moreover, it was found that this marker could be used to detect other purple pigmented rice varieties that genetically related to Thai jasmine rice. Recently, a wide variety of anthocyanin-based foods are believed to provide significant potential health benefits, and become more attractive. KDML 105 is also a Thai premier fragrant rice variety which is one of the main varieties of country’s rice export. Thus this molecular marker could be useful for commercial and breeding purposes of BKOS6 mutant and other developed varieties from KDML 105 which contain anthocyanin accumulation.展开更多
The future experimental campaign with the SAMURAI setup at RIKEN will explore a wide range of neutron-deficient nuclei with a particular focus on the most critical(p, γ) reaction rates relevant to the astrophysical r...The future experimental campaign with the SAMURAI setup at RIKEN will explore a wide range of neutron-deficient nuclei with a particular focus on the most critical(p, γ) reaction rates relevant to the astrophysical rp-process in type-I X-ray bursts(XRB). Intense radioactive-ion(RI) beams at an energy of a few hundred Me V/nucleon will be deployed to populate proton-unbound states in the nuclei of interest through the Coulomb excitation or nucleon-removal processes. The decay of these states into a proton and a heavy residue will be measured using complete kinematics and the information about time reversal proton-capture process will be obtained. This method will provide the vital experimental data on the resonances, which dominate the stellar(p, γ) reaction rates, as well as on the direct proton-capture process for some other cases. The experimental setup will utilize for the first time the High-Resolution90?-mode of the SAMURAI spectrometer in combination with the existing detection systems, including custom-designed Si-strip detectors for simultaneous detection and tracking of heavy ions and protons emitted from the target. The details of the experimental method and the utilized apparatus are discussed in this paper.展开更多
The paper includes discussions on the important role of neutron and alpha configurations in proton-rich nuclei in nuclear astrophysics in terms of nucleosynthesis under extremely high-temperature hydrogenburning condi...The paper includes discussions on the important role of neutron and alpha configurations in proton-rich nuclei in nuclear astrophysics in terms of nucleosynthesis under extremely high-temperature hydrogenburning conditions. The νp-process, which is supposed to take place at the very early epoch of type II supernovae, has considerable neutrons and alphas together with protons. The alpha-induced reactions on proton-rich unstable nuclei in the light mass regions is expected to play a crucial role, but very few of them were investigated well yet because of the experimental difficulties. Specifically, I report our recent experimental effort for the breakout process from the pp-chain region,~7Be(α, γ)^(11)C(α,p)^(14)N under the νp-process. The neutron-induced reactions on proton-rich nuclei, which is even more a challenging subject, were investigated previously for very few nuclei. One possible experimental method is the Trojan Horse Method(THM). We successfully have applied THM to the ^(18)F(n,α)^(14)N reaction study with an unstable beam of ^(18)F.展开更多
基金supported by National Natural Science Foundation of China (No.10505018)Natural Science Foundation of Henan Province (No.511030400)
文摘Retrotransposon-like elements are major constituents of most eukaryotic genomes. For example, they account for roughly 90% of the wheat （Triticum aestivum） genome, Previous study on a wheat strain treated by low-energy N^＋ ions indicated the variations in AFLP （Amplified Fragment Length Polymorphism ） markers, One such variation was caused by the re-activation of Tyl-copia-like retrotransposons, implying that the mutagenic effects of lowenergy ions might work through elevated activation of retrotransposons, In this paper an expression profile of Tyl-copia-like retrotransposons in wheat treated by low-energy N^＋ ions is reported, The reverse transcriptase （RT） domains of these retrotransposons were amplified by reverse-transcriptional polymerase chain reaction （RT-PCR） and sequentially cloned, 42 and 65 clones were obtained from the treated （CL） and control materials （CK）, respectively, Sequence analysis of each clone was performed by software. Phylogeny and classification were calculated responding to the sequences of the RT domains. All the results show that there is much difference in the RT domain between the control sample and the treated sample, Especially, the RT domains from the treated group encode significantly more functional ORF （open reading frames） than those from the control sample, This observation suggests that the treated sample has higher activation of retrotransposons, possibly as a consequence of low-energy ion beam irradiation, It also suggests that retrotransposons in the two groups impact the host gene expression in two different ways and carry out different functions in wheat cells.
基金a scholarship from the China Scholarship Council (CSC)financial support from PSI for her stay at PSI
文摘The low-energy muon facility at PSI provides nearly fully polarized positive muons with tunable energies in the ke V range to carry out muon spin rotation(LE-μSR)experiments with nanometer depth resolution on thin films,heterostructures, and near-surface regions. The low-energy muon beam is focused and transported to the sample by electrostatic lenses. In order to achieve a minimum beam spot size at the sample position and to enable the steering of the beam in the horizontal and vertical direction, a special electrostatic device has been implemented close to the sample position. It consists of a cylinder at ground potential followed by four conically shaped electrodes,which can be operated at different electric potential. In LE-μSR experiments, an electric field at the sample along the beam direction can be applied to accelerate/decelerate muons to different energies(0.5–30 keV). Additionally, a horizontal or vertical magnetic field can be superimposed for transverse or longitudinal field μSR experiments. The focusing properties of the conical lens in the presence of these additional electric and magnetic fields have been investigated and optimized by Geant4 simulations. Some experimental tests were also performed and show that the simulation well describes the experimental setup.
基金Key Innovative Project of Chinese Academy of Sciences(No.KSCX2-SW-324)Director's Foundation of the Institute of Plasma Physics(No.152900500301)
文摘A screen for Arabidopsis fertility mutants, mutagenized by low-energy argon ion beam, yielded two partial male-sterile mutants tc243-1 and tc243-2 which have similar phenotypes. tc243-2 was investigated in detail. The segregation ratio of the mutant phenotypes in the M2 pools suggested that mutation behaved as single Mendelian recessive mutations, tc243 showed a series of mutant phenotypes, among which partial male-sterile was its striking mutant characteristic. Phenotype analysis indicates that there are four factors leading to male sterility, a. Floral organs normally develop inside the closed bud, but the anther filaments do not elongate sufficiently to position the locules above the stigma at anthesis, b. The anther locules do not dehisce at the time of flower opening （although limited dehiscence occurs later）, c. Pollens of mutant plants develop into several types of pollens at the trinucleated stage. as determined by staining with DAPI （4＇,6-diamidino-2-phenylindole）. which shows a variable size. shape and number of nucleus. d. The viability of pollens is lower than that of the wild type on the germination test in vivo and vitro.
基金supported in part by the National Key Research and Development Program of China(Grant No.2020YFB1807205)in part by the National Natural Science Foundation of China(Grant No.62031019)in part by the European Commission through the H2020-MSCA-ITN META WIRELESS Research Project under Grant 956256。
文摘Reconfigurable intelligent surface(RIS)is more likely to develop into extremely large-scale RIS(XL-RIS)to efficiently boost the system capacity for future 6 G communications.Beam training is an effective way to acquire channel state information(CSI)for XL-RIS.Existing beam training schemes rely on the far-field codebook.However,due to the large aperture of XL-RIS,the scatters are more likely to be in the near-field region of XL-RIS.The far-field codebook mismatches the near-field channel model.Thus,the existing far-field beam training scheme will cause severe performance loss in the XL-RIS assisted nearfield communications.To solve this problem,we propose the efficient near-field beam training schemes by designing the near-field codebook to match the nearfield channel model.Specifically,we firstly design the near-field codebook by considering the near-field cascaded array steering vector of XL-RIS.Then,the optimal codeword for XL-RIS is obtained by the exhausted training procedure.To reduce the beam training overhead,we further design a hierarchical nearfield codebook and propose the corresponding hierarchical near-field beam training scheme,where different levels of sub-codebooks are searched in turn with reduced codebook size.Simulation results show the proposed near-field beam training schemes outperform the existing far-field beam training scheme.
文摘This paper describes a real-time beam tuning method with an improved asynchronous advantage actor–critic(A3C)algorithm for accelerator systems.The operating parameters of devices are usually inconsistent with the predictions of physical designs because of errors in mechanical matching and installation.Therefore,parameter optimization methods such as pointwise scanning,evolutionary algorithms(EAs),and robust conjugate direction search are widely used in beam tuning to compensate for this inconsistency.However,it is difficult for them to deal with a large number of discrete local optima.The A3C algorithm,which has been applied in the automated control field,provides an approach for improving multi-dimensional optimization.The A3C algorithm is introduced and improved for the real-time beam tuning code for accelerators.Experiments in which optimization is achieved by using pointwise scanning,the genetic algorithm(one kind of EAs),and the A3C-algorithm are conducted and compared to optimize the currents of four steering magnets and two solenoids in the low-energy beam transport section(LEBT)of the Xi’an Proton Application Facility.Optimal currents are determined when the highest transmission of a radio frequency quadrupole(RFQ)accelerator downstream of the LEBT is achieved.The optimal work points of the tuned accelerator were obtained with currents of 0 A,0 A,0 A,and 0.1 A,for the four steering magnets,and 107 A and 96 A for the two solenoids.Furthermore,the highest transmission of the RFQ was 91.2%.Meanwhile,the lower time required for the optimization with the A3C algorithm was successfully verified.Optimization with the A3C algorithm consumed 42%and 78%less time than pointwise scanning with random initialization and pre-trained initialization of weights,respectively.
文摘The potential of SCAR marker for discrimination of a Thai jasmine rice (Oryza sativa L. cv. KDML 105) mutant, BKOS6, obtained from ion-beam-induced mutation, was evaluated. The improved rice variety, BKOS6, exhibited many remarkable characteristics which fitted the multiple cropping system characteristics of progressive agriculture including photoperiod insensitivity, early flowering, short in stature, and purple pigment accumulation in pericarp. The BKOS6 rice grain extract has already been proved that it exhibited higher antioxidant properties than the KDML 105 and other tested rice grain extracts. In this study, the BKOS6 specific SCAR marker was developed by HAT-RAPD analysis of rice genomic DNA. The marker was successfully used to identify BKOS6 variety and its hybrid varieties containing purple pigment accumulation in plant tissues. Moreover, it was found that this marker could be used to detect other purple pigmented rice varieties that genetically related to Thai jasmine rice. Recently, a wide variety of anthocyanin-based foods are believed to provide significant potential health benefits, and become more attractive. KDML 105 is also a Thai premier fragrant rice variety which is one of the main varieties of country’s rice export. Thus this molecular marker could be useful for commercial and breeding purposes of BKOS6 mutant and other developed varieties from KDML 105 which contain anthocyanin accumulation.
文摘The future experimental campaign with the SAMURAI setup at RIKEN will explore a wide range of neutron-deficient nuclei with a particular focus on the most critical(p, γ) reaction rates relevant to the astrophysical rp-process in type-I X-ray bursts(XRB). Intense radioactive-ion(RI) beams at an energy of a few hundred Me V/nucleon will be deployed to populate proton-unbound states in the nuclei of interest through the Coulomb excitation or nucleon-removal processes. The decay of these states into a proton and a heavy residue will be measured using complete kinematics and the information about time reversal proton-capture process will be obtained. This method will provide the vital experimental data on the resonances, which dominate the stellar(p, γ) reaction rates, as well as on the direct proton-capture process for some other cases. The experimental setup will utilize for the first time the High-Resolution90?-mode of the SAMURAI spectrometer in combination with the existing detection systems, including custom-designed Si-strip detectors for simultaneous detection and tracking of heavy ions and protons emitted from the target. The details of the experimental method and the utilized apparatus are discussed in this paper.
基金Japan Society for the Promotion of Science(JSPS)Research(KAKENHI)(26287058)
文摘The paper includes discussions on the important role of neutron and alpha configurations in proton-rich nuclei in nuclear astrophysics in terms of nucleosynthesis under extremely high-temperature hydrogenburning conditions. The νp-process, which is supposed to take place at the very early epoch of type II supernovae, has considerable neutrons and alphas together with protons. The alpha-induced reactions on proton-rich unstable nuclei in the light mass regions is expected to play a crucial role, but very few of them were investigated well yet because of the experimental difficulties. Specifically, I report our recent experimental effort for the breakout process from the pp-chain region,~7Be(α, γ)^(11)C(α,p)^(14)N under the νp-process. The neutron-induced reactions on proton-rich nuclei, which is even more a challenging subject, were investigated previously for very few nuclei. One possible experimental method is the Trojan Horse Method(THM). We successfully have applied THM to the ^(18)F(n,α)^(14)N reaction study with an unstable beam of ^(18)F.
