Objective To comparatively observe the effect of electroacupuncture at digestive system-related lower he-sea points on the expressions of serum interleukin-1β(IL-1 β), tumor necrosis factor-α(TNF-α) of colon t...Objective To comparatively observe the effect of electroacupuncture at digestive system-related lower he-sea points on the expressions of serum interleukin-1β(IL-1 β), tumor necrosis factor-α(TNF-α) of colon tissues and high mobility group box 1 protein(HMGB 1) of ulcerative colitis(UC) model rats, and to explore whether there is relative specificity of electroacupuncture at Shàngjùxū(上巨虚 ST 37), one of lower he-sea points of large intestine, in treatment of bowel diseases. Method A total of 60 SD rats were randomly divided into control group, model group, ST 37 group, Zúsānl?(足三里 ST 36) group, Xiàjùxū(下巨虚 ST 39) group and Yánglíngquán(阳陵泉 GB 34) group. There were ten rats in each group; five were males, and five were females. UC models were established by clysis with 2, 4, 6-trinitrobenzene sulfonic acid/alcohol solution. After modeling, treatment was conducted for ten days, specimens were collected, colonic ulcers and inflammation were inspected visually and scored. The content of serum IL-1β and the expressions of TNF-α and HMGB 1 in colon were detected through ELISA. Results 1 Compared with control group, the scores of colonic ulcers and inflammation, the content of serum IL-1β and the expressions of TNF-α(except ST 37 group) and HMGB 1 were all higher(P〈0.05, P〈0.01); 2 compared with model group, the scores of colonic ulcers in ST 36 group and ST 37 group were lower obviously(P〈0.05, P〈0.01); the expressions of IL-1β, TNF-α and HMGB 1 in the four treatment groups were lower obviously(P〈0.01); 3 compared with ST 37 group, the expressions of IL-1β, TNF-α and HMGB 1 in other three treatment groups were higher obviously(P〈0.05, P〈0.01); and the scores of colonic ulcers in ST 39 group and GB 34 group were higher obviously(P〈0.05). Conclusion 1 The score of colonic ulcers can be reduced through electroacupuncture at ST 37, ST 36, ST 39 and GB 34, which can also reduce the content of serum IL-1β and the expressions of TNF-α and HMGB 1, and effectively inhibit inflammatory response of colon caused by UC; 2 the effect trend of the four acupoints in treatment of UC is: ST 37ST 36ST 39GB 34, and electroacupuncture at ST 37 has the best effect with relative specificity.展开更多
Objective To contrastively observe the effect of electroacupuncture at lower he-sea points of stomach, large intestine, small intestine and gallbladder of acute gastric mucosal lesion(AGML) model rats on the express...Objective To contrastively observe the effect of electroacupuncture at lower he-sea points of stomach, large intestine, small intestine and gallbladder of acute gastric mucosal lesion(AGML) model rats on the expression of serum high mobility group protein B 1(HMGB 1), tumor necrosis factor-α(TNF-α) and nuclear factor-κB(NF-κB) of gastric tissue, and to explore whether the lower he-sea points of stomach have relative specificity in treatment of stomach diseases. Methods Seventy healthy SD rats were randomly divided into seven groups, including control group, model group, "Zúsānlǐ"(足三里 ST 36) group, "Shàngjùxū"(上巨虚 ST 37) group, "Xiàjùxū"(下巨虚 ST 39) group, "Yánglíngquán"(阳陵泉 GB 34) group and "Chéngjīn"(承筋 BL 56) group, with five male rats and five female rats in each group. Acute gastric mucosal lesion model was established by applying WRS method. After successful modeling and treatment for ten days, sampling was conducted, gastric mucosal lesion was observed visually and scored, the levels of serum HMGB 1 and gastric tissue TNF-α were detected by ELISA method, and the expression of NF-κB was detected by immunohistochemical method. Results①Compared with the model group, the levels of Ulcers and inflammation(UI) in gastric mucosal and serum HMGB1, and the expression of gastric tissue TNF-α and NF-κB in the four lower he-sea points groups reduced(P〈0.01 or P〈0.05);② compared with ST 36 group, the levels of gastric mucosal UI and serum HMGB1, and the expression of gastric mucosal TNF-α and NF-κB in other treatment groups increased significantly, and the difference was statistically significant(P〈0.01 or P〈0.05). Conclusion①Electroacupuncture at relevant lower he-sea points can inhibit the body's immune response, relieve inflammatory reaction and reduce mucosal lesion by regulating HMGB 1, TNF-α and NF-κB, so as to realize the intervention and treatment on AGML rats;② the total intervention effect in ST 36 group is superior to that in other groups, indicating partially that there is a relative specificity between ST 36 and stomach.展开更多
Objective:To observe the effect of electroacupuncture(EA)at Lower He-Sea points on the expression levels of interleukin-1β(IL-1β)in the serum and gallbladder tissues,and nuclear factor-κB(NF-κB)in gallbladder tiss...Objective:To observe the effect of electroacupuncture(EA)at Lower He-Sea points on the expression levels of interleukin-1β(IL-1β)in the serum and gallbladder tissues,and nuclear factor-κB(NF-κB)in gallbladder tissues of the guinea pigs with acute cholecystitis(AC),and to explore whether Yanglingquan(GB 34),the Lower He-Sea point pertaining to Dan Fu(gallbladder),is relatively specific for the Dan Fu(gallbladder)disorders.Methods:Eighty-two healthy guinea pigs were randomly divided into 6 groups according to the random number table method,a blank group,a model group,a Yanglingquan(GB 34)group,a Zusanli(ST 36)group,a Shangjuxu(ST 37)group,and a Xiajuxu(ST 39)group,with 12 guinea pigs in the blank group while 14 in the other groups,respectively,half males and half females in each group.Except for the blank group,guinea pigs in the other groups were injected with E.coli into the gallbladder to establish AC models.Guinea pigs in the blank group were fed routinely without special treatment;those in the model group were daily tied up for 30 min without EA treatment;those in the 4 groups receiving EA treatment were acupunctured at the corresponding Lower He-Sea points after daily binding and stimulated with the SDZ-V EA instrument.After successful modeling and treatment for 5 d,blood was collected from the abdominal aorta of the guinea pigs,and the gallbladder tissues in each group were isolated for hematoxylin-eosin(HE)staining to observe the morphological changes.Enzyme-linked immunosorbent assay(ELISA)was used to detect the serum IL-1βlevel,and immunohistochemistry(IHC)was used to detect the expression levels of NF-κB and IL-1βin gallbladder.Results:On the 3rd day after modeling,the guinea pigs in the five groups with modeling were mentally depressed with decreased appetite,significantly reduced activities,slouch,lassitude,slack and matted fur,and loose stools;two guinea pigs were selected from each group(one male and one female,not included in the final statistics)to isolate the gallbladder after sacrifice;macroscopic observation showed that the gallbladder wall was differently thickened;the bile color was dark green and opaque with particles suspended or accumulated;light microscope observation showed that the submucosal blood vessels of the gallbladder were congested,along with mucosal edema,ulceration,necrosis,shedding,and a large number of inflammatory cells infiltrating in the lamina propria,indicating that the AC model was successfully prepared.Compared with the model group,the gallbladder tissue injuries of the four groups receiving EA treatment were all differently repaired,the serum IL-1βlevels were reduced(P<0.01 or P<0.05),and the IL-1βlevels in the gallbladder tissues were reduced(P<0.01 or P<0.05).Compared with the model group,the NF-κB expression level in the Yanglingquan(GB 34)group was significantly reduced(P<0.01),but was not statistical different in the Zusanli(ST 36)group,Shangjuxu(ST 37)group and Xiajuxu(ST 39)group(all P>0.05).Compared with the Yanglingquan(GB 34)group,the gallbladder tissues of the Zusanli(ST 36)group,Shangjuxu(ST 37)group and Xiajuxu(ST 39)group were more severely damaged,and the expression levels of serum IL-1β,the NF-κB and IL-1βin the gallbladder tissues were increased(P<0.01 or P<0.05).Intervention effect of Yanglingquan(GB 34)on AC guinea pigs was superior to that of Zusanli(ST 36),Shangjuxu(ST 37)and Xiajuxu(ST 39).Conclusion:EA at the Lower He-Sea points of the stomach,large intestine,small intestine and gallbladder can produce curative effects on AC guinea pigs and reduced the inflammatory symptoms.Intervention effect of Yanglingquan(GB 34)on AC guinea pigs is superior to that of Zusanli(ST 36),Shangjuxu(ST 37)and Xiajuxu(ST 39).The mechanism of EA at Yanglingquan(GB 34)in treating AC may be regulating IL-1βand NF-κB to control the inflammatory response and improve the gallbladder tissue damage.展开更多
In this paper, we use the well known KKM type theorem for generalized convex spaces due to Park (Elements of the KKM theory for generalized convex spaces, Korean J. Comp. Appl. Math., 7(2000), 1-28) to obtain an a...In this paper, we use the well known KKM type theorem for generalized convex spaces due to Park (Elements of the KKM theory for generalized convex spaces, Korean J. Comp. Appl. Math., 7(2000), 1-28) to obtain an almost fixed point theorem for upper [resp., lower] semicontinuous multimaps in locally G-convex spaces, and then give a fixed point theorem for upper semicontinuous multimap with closed Γ-convex values.展开更多
To explore lower rotating potint nutrient vessels of sural nerve flap with distant pedicled repairing the soft tissue defect of foot and ankle.Methods Lay a foundation of anatomic studying from february 2003 to March ...To explore lower rotating potint nutrient vessels of sural nerve flap with distant pedicled repairing the soft tissue defect of foot and ankle.Methods Lay a foundation of anatomic studying from february 2003 to March 2004,using lower rotating point nutrient vessels of sural nerve flap with distant pedicled repairing the soft tissue defect of foot and ankle in 11 cases.Cause of injuring:traffic accident 7 cases,crushing 1 case,saw injury 1 case,skin cancer 1 case,chronic ulcer 1 case.Areas:foot heel 6 cases,shank lower section 2 cases,heel tendon 2 cases,the distant back of the foot 1 case.Using the flap axis point was 1~3 cm above the pin of the external heel,average 2 cm.The scope of the flap was 6.0 cm×8.0 cm~12.0 cm~18.0 cm.Results All sural nerve flaps were alive.Of them,2 cases have distant part necrosis,accompanying with subcutaneous tissue,1 case heels after change dressings,another heels after skin grafting.All case can walk as usual,the flap was wear-resisting and keenly feel.Conclusion Lower rotating point nutrient vessels of sural nerve flap,donner area was fine,available area was large,skin in the pink,easy grafting,without main blood vessel damage,survival rate high,it is a good donner area in repairing around heel,foot and shank lower section.7 refs,1 tab.展开更多
With the advancement of micro machining technology,the high-heat-flux removal from miniature electronic devices and components has become an attractive topic.Flow boiling in micro-channels is an optimal form of heat t...With the advancement of micro machining technology,the high-heat-flux removal from miniature electronic devices and components has become an attractive topic.Flow boiling in micro-channels is an optimal form of heat transfer and has been widely employed in high-heat-flux cooling applications.This comprehensively-reviewed article focused on the available recent literatures of experimental investigation regarding the flow boiling heat transfer and unstable behaviors of the fluid with lower boiling point in micro-channels.The thermal-fluid characteristics and potential heat transfer mechanisms of low-boiling-point fluids flow boiling in different narrow passages were summarized and discussed.The literatures regarding the pressure drop and occurrence of the unstable phenomena existing in two-phase flow boiling process were also discussed.The emphasis was given to the heat transfer enhancement methods as well as instability elimination,and various methods such as modification of surface and channel flow geometries were considered.Some future researches in the field of micro-scale flow boiling were suggested.展开更多
目的观察激痛点火针疗法对腰背肌筋膜炎患者疼痛的影响。方法选取2021年3月—2023年3月在福建中医药大学附属三明市中西医结合医院康复科就诊的腰背肌筋膜炎患者65例,按随机数字表法分为对照组(n=32)与观察组(n=33)。对照组采用常规针...目的观察激痛点火针疗法对腰背肌筋膜炎患者疼痛的影响。方法选取2021年3月—2023年3月在福建中医药大学附属三明市中西医结合医院康复科就诊的腰背肌筋膜炎患者65例,按随机数字表法分为对照组(n=32)与观察组(n=33)。对照组采用常规针刺治疗,观察组采用针刺联合激痛点火针疗法。6周后评估2组患者疼痛评分、腰椎功能评分,观察腰背部红外成像结果。结果观察组患者视觉模拟评分法(visual analog scale,VAS)评分[(3.76±1.08)分vs.(5.28±1.55)分],Oswestry功能障碍指数问卷(Oswestry disability index,ODI)评分[(13.76±3.42)分vs.(11.50±4.58)分]及腰背部红外成像的平均温度[(30.21±3.03)℃vs.(33.31±3.16)℃]均显著低于对照组,差异有统计学意义(P<0.05)。结论针刺联合激痛点火针疗法可以有效缓解腰背肌筋膜炎患者疼痛,改善腰椎功能障碍。展开更多
基金Supported by National Natural Science Foundation of China:81173327/H2718
文摘Objective To comparatively observe the effect of electroacupuncture at digestive system-related lower he-sea points on the expressions of serum interleukin-1β(IL-1 β), tumor necrosis factor-α(TNF-α) of colon tissues and high mobility group box 1 protein(HMGB 1) of ulcerative colitis(UC) model rats, and to explore whether there is relative specificity of electroacupuncture at Shàngjùxū(上巨虚 ST 37), one of lower he-sea points of large intestine, in treatment of bowel diseases. Method A total of 60 SD rats were randomly divided into control group, model group, ST 37 group, Zúsānl?(足三里 ST 36) group, Xiàjùxū(下巨虚 ST 39) group and Yánglíngquán(阳陵泉 GB 34) group. There were ten rats in each group; five were males, and five were females. UC models were established by clysis with 2, 4, 6-trinitrobenzene sulfonic acid/alcohol solution. After modeling, treatment was conducted for ten days, specimens were collected, colonic ulcers and inflammation were inspected visually and scored. The content of serum IL-1β and the expressions of TNF-α and HMGB 1 in colon were detected through ELISA. Results 1 Compared with control group, the scores of colonic ulcers and inflammation, the content of serum IL-1β and the expressions of TNF-α(except ST 37 group) and HMGB 1 were all higher(P〈0.05, P〈0.01); 2 compared with model group, the scores of colonic ulcers in ST 36 group and ST 37 group were lower obviously(P〈0.05, P〈0.01); the expressions of IL-1β, TNF-α and HMGB 1 in the four treatment groups were lower obviously(P〈0.01); 3 compared with ST 37 group, the expressions of IL-1β, TNF-α and HMGB 1 in other three treatment groups were higher obviously(P〈0.05, P〈0.01); and the scores of colonic ulcers in ST 39 group and GB 34 group were higher obviously(P〈0.05). Conclusion 1 The score of colonic ulcers can be reduced through electroacupuncture at ST 37, ST 36, ST 39 and GB 34, which can also reduce the content of serum IL-1β and the expressions of TNF-α and HMGB 1, and effectively inhibit inflammatory response of colon caused by UC; 2 the effect trend of the four acupoints in treatment of UC is: ST 37ST 36ST 39GB 34, and electroacupuncture at ST 37 has the best effect with relative specificity.
基金Supported by:National Natural Science Foundation of China(81173327,81303048)
文摘Objective To contrastively observe the effect of electroacupuncture at lower he-sea points of stomach, large intestine, small intestine and gallbladder of acute gastric mucosal lesion(AGML) model rats on the expression of serum high mobility group protein B 1(HMGB 1), tumor necrosis factor-α(TNF-α) and nuclear factor-κB(NF-κB) of gastric tissue, and to explore whether the lower he-sea points of stomach have relative specificity in treatment of stomach diseases. Methods Seventy healthy SD rats were randomly divided into seven groups, including control group, model group, "Zúsānlǐ"(足三里 ST 36) group, "Shàngjùxū"(上巨虚 ST 37) group, "Xiàjùxū"(下巨虚 ST 39) group, "Yánglíngquán"(阳陵泉 GB 34) group and "Chéngjīn"(承筋 BL 56) group, with five male rats and five female rats in each group. Acute gastric mucosal lesion model was established by applying WRS method. After successful modeling and treatment for ten days, sampling was conducted, gastric mucosal lesion was observed visually and scored, the levels of serum HMGB 1 and gastric tissue TNF-α were detected by ELISA method, and the expression of NF-κB was detected by immunohistochemical method. Results①Compared with the model group, the levels of Ulcers and inflammation(UI) in gastric mucosal and serum HMGB1, and the expression of gastric tissue TNF-α and NF-κB in the four lower he-sea points groups reduced(P〈0.01 or P〈0.05);② compared with ST 36 group, the levels of gastric mucosal UI and serum HMGB1, and the expression of gastric mucosal TNF-α and NF-κB in other treatment groups increased significantly, and the difference was statistically significant(P〈0.01 or P〈0.05). Conclusion①Electroacupuncture at relevant lower he-sea points can inhibit the body's immune response, relieve inflammatory reaction and reduce mucosal lesion by regulating HMGB 1, TNF-α and NF-κB, so as to realize the intervention and treatment on AGML rats;② the total intervention effect in ST 36 group is superior to that in other groups, indicating partially that there is a relative specificity between ST 36 and stomach.
文摘Objective:To observe the effect of electroacupuncture(EA)at Lower He-Sea points on the expression levels of interleukin-1β(IL-1β)in the serum and gallbladder tissues,and nuclear factor-κB(NF-κB)in gallbladder tissues of the guinea pigs with acute cholecystitis(AC),and to explore whether Yanglingquan(GB 34),the Lower He-Sea point pertaining to Dan Fu(gallbladder),is relatively specific for the Dan Fu(gallbladder)disorders.Methods:Eighty-two healthy guinea pigs were randomly divided into 6 groups according to the random number table method,a blank group,a model group,a Yanglingquan(GB 34)group,a Zusanli(ST 36)group,a Shangjuxu(ST 37)group,and a Xiajuxu(ST 39)group,with 12 guinea pigs in the blank group while 14 in the other groups,respectively,half males and half females in each group.Except for the blank group,guinea pigs in the other groups were injected with E.coli into the gallbladder to establish AC models.Guinea pigs in the blank group were fed routinely without special treatment;those in the model group were daily tied up for 30 min without EA treatment;those in the 4 groups receiving EA treatment were acupunctured at the corresponding Lower He-Sea points after daily binding and stimulated with the SDZ-V EA instrument.After successful modeling and treatment for 5 d,blood was collected from the abdominal aorta of the guinea pigs,and the gallbladder tissues in each group were isolated for hematoxylin-eosin(HE)staining to observe the morphological changes.Enzyme-linked immunosorbent assay(ELISA)was used to detect the serum IL-1βlevel,and immunohistochemistry(IHC)was used to detect the expression levels of NF-κB and IL-1βin gallbladder.Results:On the 3rd day after modeling,the guinea pigs in the five groups with modeling were mentally depressed with decreased appetite,significantly reduced activities,slouch,lassitude,slack and matted fur,and loose stools;two guinea pigs were selected from each group(one male and one female,not included in the final statistics)to isolate the gallbladder after sacrifice;macroscopic observation showed that the gallbladder wall was differently thickened;the bile color was dark green and opaque with particles suspended or accumulated;light microscope observation showed that the submucosal blood vessels of the gallbladder were congested,along with mucosal edema,ulceration,necrosis,shedding,and a large number of inflammatory cells infiltrating in the lamina propria,indicating that the AC model was successfully prepared.Compared with the model group,the gallbladder tissue injuries of the four groups receiving EA treatment were all differently repaired,the serum IL-1βlevels were reduced(P<0.01 or P<0.05),and the IL-1βlevels in the gallbladder tissues were reduced(P<0.01 or P<0.05).Compared with the model group,the NF-κB expression level in the Yanglingquan(GB 34)group was significantly reduced(P<0.01),but was not statistical different in the Zusanli(ST 36)group,Shangjuxu(ST 37)group and Xiajuxu(ST 39)group(all P>0.05).Compared with the Yanglingquan(GB 34)group,the gallbladder tissues of the Zusanli(ST 36)group,Shangjuxu(ST 37)group and Xiajuxu(ST 39)group were more severely damaged,and the expression levels of serum IL-1β,the NF-κB and IL-1βin the gallbladder tissues were increased(P<0.01 or P<0.05).Intervention effect of Yanglingquan(GB 34)on AC guinea pigs was superior to that of Zusanli(ST 36),Shangjuxu(ST 37)and Xiajuxu(ST 39).Conclusion:EA at the Lower He-Sea points of the stomach,large intestine,small intestine and gallbladder can produce curative effects on AC guinea pigs and reduced the inflammatory symptoms.Intervention effect of Yanglingquan(GB 34)on AC guinea pigs is superior to that of Zusanli(ST 36),Shangjuxu(ST 37)and Xiajuxu(ST 39).The mechanism of EA at Yanglingquan(GB 34)in treating AC may be regulating IL-1βand NF-κB to control the inflammatory response and improve the gallbladder tissue damage.
文摘In this paper, we use the well known KKM type theorem for generalized convex spaces due to Park (Elements of the KKM theory for generalized convex spaces, Korean J. Comp. Appl. Math., 7(2000), 1-28) to obtain an almost fixed point theorem for upper [resp., lower] semicontinuous multimaps in locally G-convex spaces, and then give a fixed point theorem for upper semicontinuous multimap with closed Γ-convex values.
文摘To explore lower rotating potint nutrient vessels of sural nerve flap with distant pedicled repairing the soft tissue defect of foot and ankle.Methods Lay a foundation of anatomic studying from february 2003 to March 2004,using lower rotating point nutrient vessels of sural nerve flap with distant pedicled repairing the soft tissue defect of foot and ankle in 11 cases.Cause of injuring:traffic accident 7 cases,crushing 1 case,saw injury 1 case,skin cancer 1 case,chronic ulcer 1 case.Areas:foot heel 6 cases,shank lower section 2 cases,heel tendon 2 cases,the distant back of the foot 1 case.Using the flap axis point was 1~3 cm above the pin of the external heel,average 2 cm.The scope of the flap was 6.0 cm×8.0 cm~12.0 cm~18.0 cm.Results All sural nerve flaps were alive.Of them,2 cases have distant part necrosis,accompanying with subcutaneous tissue,1 case heels after change dressings,another heels after skin grafting.All case can walk as usual,the flap was wear-resisting and keenly feel.Conclusion Lower rotating point nutrient vessels of sural nerve flap,donner area was fine,available area was large,skin in the pink,easy grafting,without main blood vessel damage,survival rate high,it is a good donner area in repairing around heel,foot and shank lower section.7 refs,1 tab.
基金supported by the National Natural Science Foundation of China (No.U2141219)。
文摘With the advancement of micro machining technology,the high-heat-flux removal from miniature electronic devices and components has become an attractive topic.Flow boiling in micro-channels is an optimal form of heat transfer and has been widely employed in high-heat-flux cooling applications.This comprehensively-reviewed article focused on the available recent literatures of experimental investigation regarding the flow boiling heat transfer and unstable behaviors of the fluid with lower boiling point in micro-channels.The thermal-fluid characteristics and potential heat transfer mechanisms of low-boiling-point fluids flow boiling in different narrow passages were summarized and discussed.The literatures regarding the pressure drop and occurrence of the unstable phenomena existing in two-phase flow boiling process were also discussed.The emphasis was given to the heat transfer enhancement methods as well as instability elimination,and various methods such as modification of surface and channel flow geometries were considered.Some future researches in the field of micro-scale flow boiling were suggested.
文摘目的观察激痛点火针疗法对腰背肌筋膜炎患者疼痛的影响。方法选取2021年3月—2023年3月在福建中医药大学附属三明市中西医结合医院康复科就诊的腰背肌筋膜炎患者65例,按随机数字表法分为对照组(n=32)与观察组(n=33)。对照组采用常规针刺治疗,观察组采用针刺联合激痛点火针疗法。6周后评估2组患者疼痛评分、腰椎功能评分,观察腰背部红外成像结果。结果观察组患者视觉模拟评分法(visual analog scale,VAS)评分[(3.76±1.08)分vs.(5.28±1.55)分],Oswestry功能障碍指数问卷(Oswestry disability index,ODI)评分[(13.76±3.42)分vs.(11.50±4.58)分]及腰背部红外成像的平均温度[(30.21±3.03)℃vs.(33.31±3.16)℃]均显著低于对照组,差异有统计学意义(P<0.05)。结论针刺联合激痛点火针疗法可以有效缓解腰背肌筋膜炎患者疼痛,改善腰椎功能障碍。