Aim: To study the effect of 5-nitro-2-fumldehyde semicarbazone (nitrofurazone), a derivative of nitrofuran, onmale reproductive organs of Parkes (P) swain mice. Methods: Mice were given nitrofurazone orally at a dose ...Aim: To study the effect of 5-nitro-2-fumldehyde semicarbazone (nitrofurazone), a derivative of nitrofuran, onmale reproductive organs of Parkes (P) swain mice. Methods: Mice were given nitrofurazone orally at a dose of 64mg/kg body weight per day, for 10 and 20 days, and were killed 24 h and/or 56 days after the last treatment. Histo-logical appearance of testis, motility and number of spermatozoa in cauda epididymidis, and biochemical indices in epi-didymis and seminal vesicle were evaluated. Results: Histologically, testis showed marked regressive changes inthe seminiferous tubules in mice treated with nitrofurazone. Ten days after treatment, there was much depletion of germcells in the seminiferous tubules, and the germinal epithelium was lined mainly with Sertoli cells, spermatogonia, sper-matocytes, and a few round spermatids; intraepithelial vacuoles and multinucleated giant cells were also observed intubules. By 20 days, regressive changes in the seminiferous tubules were further pronounced, and pachytene spermato-cytes were the most advanced germ cells noticed in the tubules. In severe cases, the tubules were lined with a thin layerof Sertoli cells and spermatogonia. The treatment also caused marked reductions in the motility and number of sperma-tozoa in the cauda epididymidis, in weight and the level of fructose in the seminal vesicle, and in sialic acid level in theepididymis. Fifty six days after drag withdrawal, the alterations induced in the reproductive organs returned to controllevels. Conclusion: Our results suggest that nitrofurazone treatment in P mice induces marked alterations in themale reproductive organs, and that the alterations are reversible following cessation of treatment.(Asian J Androl 2001 Mar; 3: 39-44)展开更多
Ecotoxic effects of antibiotics or ammonium have been confirmed independently in aquatic animals,but few studies have investigated their combined effects.In aquaculture ecosystems,these pollutants frequently coexist,a...Ecotoxic effects of antibiotics or ammonium have been confirmed independently in aquatic animals,but few studies have investigated their combined effects.In aquaculture ecosystems,these pollutants frequently coexist,and often in high concentrations.In this study,the combined effects of antibiotic nitrofurazone and NH4 Cl on the population dynamics and growth rates of two species of ciliated protists,Euplotes vannus and Pseudokeronopsis rubra,were investigated.Profiles of the dose-responses were visualized,and interactions between the two pollutants were quantified by the response surface method(RSM).Results showed that 1)the dynamics of the population growth differed significantly between the testd ciliates and varied with the concentrations of the pollutants;2)the relative growth rate(RGR)of both ciliates decreased significantly with increased pollutant concentrations,while the difference in RGR between the two ciliates was not significant;3)RSM analysis demonstrated an additive effect of nitrofurazone and NH4 Cl on the RGR in both ciliates.In brief,ecotoxic effects can be caused by nitrofurazone and ammonium independently on the two test ciliates,and such effects can be strengthened when they present at the same time.These findings offer a valuable reference for evaluating combined ecotoxic effects caused by multiple pollutants in aquaculture ecosystems.展开更多
The electrochemical behavior of nitrofurazone in dimethylformamide has been studied by cyclic voltammetry and in-situ thin-layer spectroelectrochemistry.The redox reactions are complicated at platinium electrode,and a...The electrochemical behavior of nitrofurazone in dimethylformamide has been studied by cyclic voltammetry and in-situ thin-layer spectroelectrochemistry.The redox reactions are complicated at platinium electrode,and a possible electrochemical mechanism of nitrofurazone is proposed.展开更多
文摘Aim: To study the effect of 5-nitro-2-fumldehyde semicarbazone (nitrofurazone), a derivative of nitrofuran, onmale reproductive organs of Parkes (P) swain mice. Methods: Mice were given nitrofurazone orally at a dose of 64mg/kg body weight per day, for 10 and 20 days, and were killed 24 h and/or 56 days after the last treatment. Histo-logical appearance of testis, motility and number of spermatozoa in cauda epididymidis, and biochemical indices in epi-didymis and seminal vesicle were evaluated. Results: Histologically, testis showed marked regressive changes inthe seminiferous tubules in mice treated with nitrofurazone. Ten days after treatment, there was much depletion of germcells in the seminiferous tubules, and the germinal epithelium was lined mainly with Sertoli cells, spermatogonia, sper-matocytes, and a few round spermatids; intraepithelial vacuoles and multinucleated giant cells were also observed intubules. By 20 days, regressive changes in the seminiferous tubules were further pronounced, and pachytene spermato-cytes were the most advanced germ cells noticed in the tubules. In severe cases, the tubules were lined with a thin layerof Sertoli cells and spermatogonia. The treatment also caused marked reductions in the motility and number of sperma-tozoa in the cauda epididymidis, in weight and the level of fructose in the seminal vesicle, and in sialic acid level in theepididymis. Fifty six days after drag withdrawal, the alterations induced in the reproductive organs returned to controllevels. Conclusion: Our results suggest that nitrofurazone treatment in P mice induces marked alterations in themale reproductive organs, and that the alterations are reversible following cessation of treatment.(Asian J Androl 2001 Mar; 3: 39-44)
基金supported by the National Natural Science Foundation of China(Nos.31971519,31430077,41476128)in part by a grant from the Dedicated Fund for Promoting High-Quality Economic Development in Guangdong Province(Marine Economic Development Project:GDOE(2019)A23)。
文摘Ecotoxic effects of antibiotics or ammonium have been confirmed independently in aquatic animals,but few studies have investigated their combined effects.In aquaculture ecosystems,these pollutants frequently coexist,and often in high concentrations.In this study,the combined effects of antibiotic nitrofurazone and NH4 Cl on the population dynamics and growth rates of two species of ciliated protists,Euplotes vannus and Pseudokeronopsis rubra,were investigated.Profiles of the dose-responses were visualized,and interactions between the two pollutants were quantified by the response surface method(RSM).Results showed that 1)the dynamics of the population growth differed significantly between the testd ciliates and varied with the concentrations of the pollutants;2)the relative growth rate(RGR)of both ciliates decreased significantly with increased pollutant concentrations,while the difference in RGR between the two ciliates was not significant;3)RSM analysis demonstrated an additive effect of nitrofurazone and NH4 Cl on the RGR in both ciliates.In brief,ecotoxic effects can be caused by nitrofurazone and ammonium independently on the two test ciliates,and such effects can be strengthened when they present at the same time.These findings offer a valuable reference for evaluating combined ecotoxic effects caused by multiple pollutants in aquaculture ecosystems.
文摘The electrochemical behavior of nitrofurazone in dimethylformamide has been studied by cyclic voltammetry and in-situ thin-layer spectroelectrochemistry.The redox reactions are complicated at platinium electrode,and a possible electrochemical mechanism of nitrofurazone is proposed.
