Luffaculin 1 purified from the seeds of Luffa acutangula belongs to the type I ribosome-inactivating proteins （RIPs）. It has been crystallized by the vapor-diffusion method using polyethylene glycol as a precipitant...Luffaculin 1 purified from the seeds of Luffa acutangula belongs to the type I ribosome-inactivating proteins （RIPs）. It has been crystallized by the vapor-diffusion method using polyethylene glycol as a precipitant. The crystal is of space group P1 with a = 39.135, b = 46.813, c = 83.571 A, α = 891068,β = 80.009 and y = 72.143°, and has two molecules per asymmetric unit. X-ray data have been collected to be 1.4 A using a synchrotron source.展开更多
In this study, 32 Luffa germplasm resources were collected from various regions in Zhejiang Province as experimental materials, to investigate 22 agronomic traits including fruit bearing habit, leaf margin, fruit ribb...In this study, 32 Luffa germplasm resources were collected from various regions in Zhejiang Province as experimental materials, to investigate 22 agronomic traits including fruit bearing habit, leaf margin, fruit ribbing and percentage of nodes with female flowers to total node. Based on the obtained experimental data, principal component analysis and cluster analysis were carried out using DPS software. The results showed that 22 agronomic traits could be integrated into 5 principal components, with the cumulative contributive percentage of 81. 308%. According to the correlations between the first five principal components and traits, 14 traits with great influences were screened. On the basis of principal component analysis, cluster analysis of 32 Luffa germplasm resources was conducted, which divided Luffa cylindrica and Luffa acutangula into two categories and six subcategories by Euclidean genetic distances. This study provided scientific basis for the collection, preservation, identification, creation and utilization of Luffa germplasm and parent selection in cross breeding of Luffa.展开更多
Luffa aegyptiaca fruit has been assayed for the presence of lignin peroxidase activity using veratryl alcohol as the substrate. The fruit juice contained activity of 3.14 U/ml which was much higher than 0.075 U/ml rep...Luffa aegyptiaca fruit has been assayed for the presence of lignin peroxidase activity using veratryl alcohol as the substrate. The fruit juice contained activity of 3.14 U/ml which was much higher than 0.075 U/ml reported in the culture filtrate of Phanarochaete chrysosporium ATCC-24725. The K<sub>m</sub> value of the lignin peroxidase using veratryl alcohol as the variable substrate in 50mM phosphate buffer pH 2.5 at 25°C was found to be 50 μM respectively. The pH and temperature optima of the lignin peroxidase were 2.4 and 22°C, respectively. The present article reports viable method to explore rich sources of lignin peroxidase from plants which can be used as a mediator in oxidative organic transformations within green chemistry domain ensuring ecofriendly synthesis of bioorganic molecules of pharmaceutical value.展开更多
The effect of chemical modification, reinforcement structure and fiber weight ratio on the flexural proprieties of Luffa-polyester composites was studied. A unsaturated polyester matrix reinforced with a mat of Luffa ...The effect of chemical modification, reinforcement structure and fiber weight ratio on the flexural proprieties of Luffa-polyester composites was studied. A unsaturated polyester matrix reinforced with a mat of Luffa external wall fibers (ComLEMat), a short Luffa external wall fibers(ComLEBC) and a short Luffa core fi-bers (ComLCBC) was fabricated under various conditions of fibers treatments (combined process, acetylat-ing and cyanoethylating) and fiber weight ratio. It resorts that acetylating and cyanoethylating enhance the flexural strength and the flexural modulus. The fiber weight ratio influenced the flexural properties of com-posites. Indeed, a maximum value of strength and strain is observed over a 10% fiber weight ratio. The uses of various reinforcement structures were investigated. The enhancement of elongation at break and the strain values of the composite reinforced by natural mat was proved.展开更多
The influence of cellulose nano fibers extracted from the fruit of luffa cylindrica (LC) on the tensile, flexural and impact properties of composite materials using poly lactic acid (PLA) processed by micro compoundin...The influence of cellulose nano fibers extracted from the fruit of luffa cylindrica (LC) on the tensile, flexural and impact properties of composite materials using poly lactic acid (PLA) processed by micro compounding and injection molding was studied. Preliminary results suggested promising mechanical properties. The impact strength, tensile strength and flexural strength of the composites increased with incorporation of very low content of LC fiber up to 2 wt%. But when the wt of LC fiber in the composite increased (5 wt% and 10 wt%), mechanical strength of the composites reduced probably due to agglomeration of cellulose fibers. However, modulus of composites was enhanced with increase in wt of fiber content in the composites. Before reinforcement, the LC fibers were modified with calcium phosphate in order to explore the possibilities of using these composites in biomedical industries. The novelty of this work is that there is no use of compatiblizer and coupling agent during the processing so that the cost of processing is reduced.展开更多
This paper presents the study of moisture content, hardness, bulk density, apparent porosity, tensile and flexural characteristics of composite properties of Luffa aegyptiaca fiber. Luffa aegyptiaca reinforced epoxy c...This paper presents the study of moisture content, hardness, bulk density, apparent porosity, tensile and flexural characteristics of composite properties of Luffa aegyptiaca fiber. Luffa aegyptiaca reinforced epoxy composites have been developed by hand lay-up method with Luffa fiber untreated and treated conditions for 12 Hrs and 24 Hrs in different filler loading as in 2:1 ratio (5%, 10%, 15%, 20% and 25%). The effects of filler loading on the moisture content, hardness, bulk density, apparent porosity, tensile and flexural properties were studied. In general, the treated Luffa fibre composite for 24 Hrs showed better improvement properties via addition of modified Luffa fibre as reinforcement. However, tensile and flexural properties improved continuously with increasing filler loading up to 20% but decreasing at 25% due to weak interfacial bonding for both untreated and treated composite. The favourable results were obtained at 20% for treated composite at 24 Hrs especially at tensile and flexural characteristics and are suitable for mechanical applications.展开更多
This work is realized in the context of valorizing natural and local resources, in particular, luffa plant fruit (luffa sponge). The raw fibers of the luffa sponge have a short lifetime. Hence, when they are chemicall...This work is realized in the context of valorizing natural and local resources, in particular, luffa plant fruit (luffa sponge). The raw fibers of the luffa sponge have a short lifetime. Hence, when they are chemically treated, it constitutes a solution is prepared to limit their degradation in the long term and to improve their mechanical characteristics. Therefore, this paper studies the effect of the chemical treatment on the mechanical properties of the luffa sponge’s fibers (fibers of luffa Sponge). The chemical process consists of dipping a brunch of luffa in various concentrations of sodium hydroxide (NaOH) at different time intervals and at different temperature conditions. The luffa sponge’s fibers were mechanical. Characterized before and after the treatment, mechanically (micro traction test). It has been shown that an optimum of 61% increase in mechanical properties (tensile strength) has been reached in the following conditions: treatment with 1% concentration for 90 min at 50°C.展开更多
Darwin, in his retiring years, conducted long-term studies at home on various modes of the movement of ordinary plant organs and sensitive floral parts, in climbing and twining plants, and in insectivorous plants. His...Darwin, in his retiring years, conducted long-term studies at home on various modes of the movement of ordinary plant organs and sensitive floral parts, in climbing and twining plants, and in insectivorous plants. His conclusions derived therefrom laid emphasis on the fundamental similarty in irritability between plant and animal species, thereby, Supporting his theory of the biological evolution. Since then, Darwin’s展开更多
The tendril of luffa (Luffa officinale),in response to various non-injurious and injuriousstimulations,will first give rise to electrochemical transmission including the action wave,variation wave,andwave complex.It i...The tendril of luffa (Luffa officinale),in response to various non-injurious and injuriousstimulations,will first give rise to electrochemical transmission including the action wave,variation wave,andwave complex.It is then followed by rapid coiling movement which appears within 25—30 s after stimulation.The coiling process continues for 10 min or more.When it is over,the tendril begins to uncoil itself.Tendrils pretreated with the narcotic drug,ethyl ether fail to response to stimulation entirely.Specific inhibitorsof microfilament,cytochalasins B and D,and of myosin,N-ethylmaleimide and iodoacetic acid,are both able toprevent the coiling of tendril,without any effect on the electrical wave transmission.Colchicine,known toinhibit microtubule action,is ineffective in preventing coiling.Acetylcholine is able to provoke the tendril coilingdirectly.The effects of drug pretreatments support the proposal that the electrochemical wave transmission elicitedby stimulation is the forerunner for arousing the rapid movement of the sensitive tendril that the actin and myosinare taking part in.Turgor changes in tendril cells are intimately connected with the transmitted and the motoraction of the compact tissues lining along the more sensitive ventral side of the tendril.As the ventral cellsundergo the abrupt contraction and slightly shrink,the dorsal cells become expanded in coiling.Eventually,turgotresumes normal on both sides when uncoiling occurs.展开更多
基金This work was supported by the National Natural Science Foundation of China (No. 39970872) and Natural Science Foundation of Fujian Province (C97052)
文摘Luffaculin 1 purified from the seeds of Luffa acutangula belongs to the type I ribosome-inactivating proteins （RIPs）. It has been crystallized by the vapor-diffusion method using polyethylene glycol as a precipitant. The crystal is of space group P1 with a = 39.135, b = 46.813, c = 83.571 A, α = 891068,β = 80.009 and y = 72.143°, and has two molecules per asymmetric unit. X-ray data have been collected to be 1.4 A using a synchrotron source.
基金Supported by"San Nong Liu Fang"Science and Technology Cooperation Project of Zhejiang Province(ZNJF[2011]No.85)Major Project of Science and Technology of Zhejiang Province(2009C2006-1-8)
文摘In this study, 32 Luffa germplasm resources were collected from various regions in Zhejiang Province as experimental materials, to investigate 22 agronomic traits including fruit bearing habit, leaf margin, fruit ribbing and percentage of nodes with female flowers to total node. Based on the obtained experimental data, principal component analysis and cluster analysis were carried out using DPS software. The results showed that 22 agronomic traits could be integrated into 5 principal components, with the cumulative contributive percentage of 81. 308%. According to the correlations between the first five principal components and traits, 14 traits with great influences were screened. On the basis of principal component analysis, cluster analysis of 32 Luffa germplasm resources was conducted, which divided Luffa cylindrica and Luffa acutangula into two categories and six subcategories by Euclidean genetic distances. This study provided scientific basis for the collection, preservation, identification, creation and utilization of Luffa germplasm and parent selection in cross breeding of Luffa.
文摘Luffa aegyptiaca fruit has been assayed for the presence of lignin peroxidase activity using veratryl alcohol as the substrate. The fruit juice contained activity of 3.14 U/ml which was much higher than 0.075 U/ml reported in the culture filtrate of Phanarochaete chrysosporium ATCC-24725. The K<sub>m</sub> value of the lignin peroxidase using veratryl alcohol as the variable substrate in 50mM phosphate buffer pH 2.5 at 25°C was found to be 50 μM respectively. The pH and temperature optima of the lignin peroxidase were 2.4 and 22°C, respectively. The present article reports viable method to explore rich sources of lignin peroxidase from plants which can be used as a mediator in oxidative organic transformations within green chemistry domain ensuring ecofriendly synthesis of bioorganic molecules of pharmaceutical value.
文摘The effect of chemical modification, reinforcement structure and fiber weight ratio on the flexural proprieties of Luffa-polyester composites was studied. A unsaturated polyester matrix reinforced with a mat of Luffa external wall fibers (ComLEMat), a short Luffa external wall fibers(ComLEBC) and a short Luffa core fi-bers (ComLCBC) was fabricated under various conditions of fibers treatments (combined process, acetylat-ing and cyanoethylating) and fiber weight ratio. It resorts that acetylating and cyanoethylating enhance the flexural strength and the flexural modulus. The fiber weight ratio influenced the flexural properties of com-posites. Indeed, a maximum value of strength and strain is observed over a 10% fiber weight ratio. The uses of various reinforcement structures were investigated. The enhancement of elongation at break and the strain values of the composite reinforced by natural mat was proved.
文摘The influence of cellulose nano fibers extracted from the fruit of luffa cylindrica (LC) on the tensile, flexural and impact properties of composite materials using poly lactic acid (PLA) processed by micro compounding and injection molding was studied. Preliminary results suggested promising mechanical properties. The impact strength, tensile strength and flexural strength of the composites increased with incorporation of very low content of LC fiber up to 2 wt%. But when the wt of LC fiber in the composite increased (5 wt% and 10 wt%), mechanical strength of the composites reduced probably due to agglomeration of cellulose fibers. However, modulus of composites was enhanced with increase in wt of fiber content in the composites. Before reinforcement, the LC fibers were modified with calcium phosphate in order to explore the possibilities of using these composites in biomedical industries. The novelty of this work is that there is no use of compatiblizer and coupling agent during the processing so that the cost of processing is reduced.
文摘This paper presents the study of moisture content, hardness, bulk density, apparent porosity, tensile and flexural characteristics of composite properties of Luffa aegyptiaca fiber. Luffa aegyptiaca reinforced epoxy composites have been developed by hand lay-up method with Luffa fiber untreated and treated conditions for 12 Hrs and 24 Hrs in different filler loading as in 2:1 ratio (5%, 10%, 15%, 20% and 25%). The effects of filler loading on the moisture content, hardness, bulk density, apparent porosity, tensile and flexural properties were studied. In general, the treated Luffa fibre composite for 24 Hrs showed better improvement properties via addition of modified Luffa fibre as reinforcement. However, tensile and flexural properties improved continuously with increasing filler loading up to 20% but decreasing at 25% due to weak interfacial bonding for both untreated and treated composite. The favourable results were obtained at 20% for treated composite at 24 Hrs especially at tensile and flexural characteristics and are suitable for mechanical applications.
文摘This work is realized in the context of valorizing natural and local resources, in particular, luffa plant fruit (luffa sponge). The raw fibers of the luffa sponge have a short lifetime. Hence, when they are chemically treated, it constitutes a solution is prepared to limit their degradation in the long term and to improve their mechanical characteristics. Therefore, this paper studies the effect of the chemical treatment on the mechanical properties of the luffa sponge’s fibers (fibers of luffa Sponge). The chemical process consists of dipping a brunch of luffa in various concentrations of sodium hydroxide (NaOH) at different time intervals and at different temperature conditions. The luffa sponge’s fibers were mechanical. Characterized before and after the treatment, mechanically (micro traction test). It has been shown that an optimum of 61% increase in mechanical properties (tensile strength) has been reached in the following conditions: treatment with 1% concentration for 90 min at 50°C.
基金the National Natural Science Foundation of China.
文摘Darwin, in his retiring years, conducted long-term studies at home on various modes of the movement of ordinary plant organs and sensitive floral parts, in climbing and twining plants, and in insectivorous plants. His conclusions derived therefrom laid emphasis on the fundamental similarty in irritability between plant and animal species, thereby, Supporting his theory of the biological evolution. Since then, Darwin’s
基金the National Natural Science Foundation of China.
文摘The tendril of luffa (Luffa officinale),in response to various non-injurious and injuriousstimulations,will first give rise to electrochemical transmission including the action wave,variation wave,andwave complex.It is then followed by rapid coiling movement which appears within 25—30 s after stimulation.The coiling process continues for 10 min or more.When it is over,the tendril begins to uncoil itself.Tendrils pretreated with the narcotic drug,ethyl ether fail to response to stimulation entirely.Specific inhibitorsof microfilament,cytochalasins B and D,and of myosin,N-ethylmaleimide and iodoacetic acid,are both able toprevent the coiling of tendril,without any effect on the electrical wave transmission.Colchicine,known toinhibit microtubule action,is ineffective in preventing coiling.Acetylcholine is able to provoke the tendril coilingdirectly.The effects of drug pretreatments support the proposal that the electrochemical wave transmission elicitedby stimulation is the forerunner for arousing the rapid movement of the sensitive tendril that the actin and myosinare taking part in.Turgor changes in tendril cells are intimately connected with the transmitted and the motoraction of the compact tissues lining along the more sensitive ventral side of the tendril.As the ventral cellsundergo the abrupt contraction and slightly shrink,the dorsal cells become expanded in coiling.Eventually,turgotresumes normal on both sides when uncoiling occurs.
