N-acetylcysteine and zinc sulphate abate di-2-ethylhexyl phthalate-mediated reproductive dysfunction in rats:Focus on oxidative and sex hormone receptors mechanisms

Objective:To investigate the potential of N-acetylcysteine(NAC)and zinc sulphate(ZnSO_(4))in mitigating reproductive dysfunction caused by di-2-ethylhexyl phthalate(DEHP)in rats and to understand the underlying mechanisms,specifically oxidative stress and sex hormone receptor activity.Methods:Thirty-five male Wistar rats were randomly divided into five equal groups(n=7 per group).Group 1 was administered 0.5 mL of distilled water and served as the control group.Group 2 was given only DEHP(750 mg/kg/day),while group 3,4 and 5 were given DEHP(750 mg/kg/day)plus NAC(100 mg/kg/day),DEHP(750 mg/kg/day)plus ZnSO_(4)(0.5 mg/kg/day),and DEHP(750 mg/kg/day)plus NAC(100 mg/kg/day)as well as ZnSO_(4)(0.5 mg/kg/day),respectively.All treatments lasted for 21 days.Samples were obtained after the rats were sacrificed,and hormones levels in the serum and markers of oxidative stress in the testicles were analyzed using the enzyme-linked immunosorbent assay.The amount of androgen receptors in the testicles was determined by immunohistochemistry,and the susceptibility of testosterone and DEHP to bind to androgen receptor and 5α-reductase was determined by molecular docking studies.Results:DEHP decreased reproductive hormones,testicular antioxidant enzymes,increased malondialdehyde levels,and negatively impacted histology of the pituitary and testes.NAC or ZnSO_(4) treatment showed a marked improvement in testicular antioxidant status and hormone levels,as well as a positive effect on the histology of the pituitary and testes.The combination of both treatments appeared to be more effective.The affinity of DEHP to bind to androgen receptors may lead to disruption of androgen receptor signaling,which can further result in dysfunction of hormones related to androgen.However,NAC is more likely to form stronger binding interactions with follicle stimulating hormone and luteinizing hormone receptors,as well as gonadotropin-releasing hormone receptors,when compared to DEHP.Conclusions:The possibility that NAC and ZnSO_(4) could downregulate DEHP-induced sex hormone changes is suggested by their potential to reduce toxicity.

血清HE4、LH、FSH对宫颈癌和宫颈上皮内瘤变的鉴别诊断价值

目的探究血清人附睾蛋白4(HE4)、促黄体生成素(LH)、卵泡刺激素(FSH)对宫颈癌和宫颈上皮内瘤变(CIN)的鉴别诊断价值。方法选择89例接受宫颈癌筛查的宫颈病变患者,经病理学或细胞学明确诊断宫颈癌51例、CIN 38例,检测所有患者血清HE4、LH、FSH水平,分析宫颈癌患者与CIN患者血清检测指标水平差异,采用受试者特征工作曲线(ROC曲线)、二元logistics回归分析法评价HE4、LH、FSH在疾病鉴别中的价值。结果宫颈癌患者血清HE4、LH、FSH水平均显著高于CIN患者(P<0.05);宫颈病变患者血清HE4与LH、FSH水平呈正相关(γ=0.357、0.392,P<0.05)。ROC曲线分析显示,血清HE4、LH、FSH诊断宫颈癌的敏感性均较低,三者联合诊断效能较单一指标更高,联合诊断曲线下面积为0.859,诊断敏感度88.24%、特异度71.05%;logistics回归分析显示,HE4>62.84 pmol/ml是宫颈癌发病的影响因素(P<0.05)。结论宫颈癌患者血清HE4、LH、FSH水平异常升高,且指标间呈正相关,三者联合用于鉴别诊断宫颈癌和CIN具有一定临床价值。

Influence of leptin on luteinizing hormone and follicle stimulating hormone secreted from cultured rat anterior pituitary cells

BACKGROUND： Leptin may regulate reproductive function via release of hypothalamic neuropeptide Y. However, it is unknown whether this regulatory effect is limited to the hypothalamus. OBJECTIVE： To detect the effect of different dosages of leptin on luteinizing hormone （LH） and follicle stimulating hormone （FSH） secretion from in vitro cultured rat anterior pituitary cells. DESIGN： Contrast study based on cells. SETTING： This study was performed in the Basic Institute of Chengde Medical College, Chengde City, Hebei Province, China from March to June 2007. MATERIALS： Eighteen female Wistar rats of three months of age, weighing 200-220 g, and of clean grade were used. Leptin was provided by Peprotech Company, DMEM culture medium by Invitrogen Company, and the radioimmunological kit by Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. METHODS： Three glandular organs were regarded as one group for culture of anterior pituitary cells. In the control group, saline was added to the culture medium instead of leptin. In the leptin group, leptin was prepared into different concentrations of 1×10^-12, 1×10^-11, 1×10^-9, 1×10^-7, and 1×10^-6 mol/L for stimulation of cultured cells. The culture supernatant was obtained at three hours after additional of saline/leptin. MAIN OUTCOME MEASURES： Contents of LH and FSH were detected by radioimmunology. RESULTS： Following leptin stimulation, LH release increased with increasing concentrations of leptin up to 1×10^-9 mol/L, where LH release peaked. LH release then progressively decreased with increasing leptin concentrations （P 〈 0.01）. LH release in the leptin （1×10^-12, 1×10^-11, 1×10^-7, and 1×10^-6 mol/L） groups was significantly higher than that in the control group （P 〈 0.01）. FSH content in the leptin （1×10^-11, 1×10^-9, and 1×10^-7 mol/L） groups was significantly higher than that in the control group （P 〈 0.01）. CONCLUSION： Leptin can directly affect pituitary tissue to promote the secretion of LH and FSH in a dose-dependent manner.

Combination immunotherapy with Survivin and luteinizing hormone-releasing hormone fusion protein in murine breast cancer model

AIM To investigate the therapeutic potential of two recombinant proteins, Survivin and luteinizing hormone-releasing hormone (LHRH) fusion protein [LHRH(6 leu)-LTB] for immunotherapy of breast cancer.METHODS Murine 4 T-1 breast cancer model was used to evaluate the efficacy of recombinant proteins in vivo. Twenty four Balb/c mice were divided into 4 groups of 6 mice each. Recombinant Survivin and LHRH fusion protein, alone or in combination, were administered along with immunomodulator Mycobacterium indicus pranii (MIP) in Balb/c mice. Unimmunized or control group mice were administered with phosphate buffer saline. Each group was then challenged with syngeneic 4 T-1 cells to induce the growth of breast tumor. Tumor growth was monitored to evaluate the efficacy of immune-response in preventing the growth of cancer cells.RESULTS Preventive immunization with 20 μg recombinant Survivin and MIP was effective in suppressing growth of 4 T-1 mouse model of breast cancer (P = 0.04) but 50 μg dose was ineffective in suppressing tumor growth. However, combination of Survivin and LHRH fusion protein was more effective in suppressing tumor growth (P = 0.02) as well as metastasis in vivo in comparison to LHRH fusion protein as vaccine antigen alone.CONCLUSION Recombinant Survivin and MIP suppress tumor growth significantly. Combining LHRH fusion protein with Survivin and MIP enhances tumor suppressive effects marginally which provides evidence for recombinant Survivin and LHRH fusion protein as candidates for translating the combination cancer immunotherapy approaches.

Long-term effectiveness of luteinizing hormone-releasing hormone agonist or antiandrogen monotherapy in elderly men with localizect prostate cancer （T1-2） ： a retrospective study

Aim： To evaluate the long-term effectiveness, side effects and compliance rates of two types of drugs （luteinizing hormone-releasing hormone [LHRH] agonist and antiandrogen） that were used individually to treat patients with localized prostate cancer （T1-2） at our institution. Methods： Ninety-seven patients who were diagnosed in the period from April 1997 to January 2000 as having clinically localized prostate cancer （T1-2） received either LHRH agonist （leuprolide acetate 7.5 mg/month） monotherapy （group 1, n = 62） or antiandrogen monotherapy （group 2, n = 35; 18 received bicalutamide 50 mg q.d., 13 received nilutamide 150 mg t.i.d, and 4 received flutamide 250 mg t.i.d.）. The mean age in both groups was 76 years. Results： The mean follow-up time was （50.8 ±8.5） months in group 1 and （43.1 ± 2.2） months in group 2. Prostate-specific antigen （PSA） levels rose in only 1 of the 62 patients （1.6%） in group 1, and in 20 of the 35 patients （57.1%） in group 2. In group 2, 10 of the 20 patients （50 %） with increasing PSA levels were treated with LHRH salvage therapy, and eight （80%） responded. Hot flashes （54.8%） and lethargy （41.9%） were the most common side effects in group 1. In contrast, nipple-tenderness （40%） and light-dark adaptation （17.1%） were more often seen in group 2. Only 1 of the 62 patients （1.6%） in group 1 switched to another medication because of adverse side effects; whereas 8 of the 35 patients （22.9%） in group 2 did so. Conclusion： Unlike antiandrogen monotherapy, LHRH agonist monotherapy provided long-term durable control of localized prostate cancer （T1-2）. It can also be an effective treatment option for patients whose disease failed to respond to antiandrogen monotherapy. The limitations of our study are the lack of health outcomes analysis and a small sample size.

Effect of Luteinizing Hormone-Releasing Hormone Analogue on the Sexual Behavior of Sacalia quadriocellata

Luteinizing hormone-releasing hormone(LHRH) is known to influence sexual behavior in many vertebrate taxa, but there have been no systematic studies on the role of LHRH in sexual behavior of turtles. We tested the hypotheses that exogenous LHRH analogues would induce sexual behavior of male Four-eyed turtle, Sacalia quadriocellata. We examined this by challenging males with intramuscular injections of mammalian luteinizing hormone-releasing hormone analogue(LHRH-A), human chorionic gonadotropin(HCG), or a combination of the two, and subsequently exposing them to sexually receptive females for behavioral observation. Our data show that the injection of only HCG could not, while that of only LHRH-A could, facilitate sexual behavior along with testicular recrudescence and spermatogenesis in S. quadriocellata. The injection of both LHRH-A and HCG would induce more drastic sexual behavior of the animals than that of LHRH-A alone, indicating HCG enhances the effects of LHRH-A induced sexual behavior. However, different pharmacological dosages of LHRH-A(0.5 μg, 1 μg, 2 μg per 100 g bodyweight) did not correspond to different activity levels. Though the mechanism of LHRH effect was not determined, this study may support that the sexual behavior of S. quadriocellata which occurs at the beginning of the injection despite regression of the gonads. This is the first report on the exogenous LHRH-A induced sexual behavior for this species.

Effects of Estrogen and/or Progesterone on Morphology of Pituitary Luteinizing Hormone Cells

Objective To identify the morphological characteristics of pituitary luteinizing hormone (LH) cells after exogenous gonadal hormone treatment Methods Effects of various doses of estrogen, progesterone and their combination on morphological parameters, including the size and shape of pituitary LH cells, the size of endocellular vacuoles, were observed and measured by immuno histochemistry and computer image analysis. Results Different kinds of gonadal hormones could recover the magnified LH cells to the normal level in ovariectomized rats. However, their final effects on the gonadotrophin levels and the cellular morphological characters of the LH cells were different. The low dose of estrogen elicited abundant hormone stored in the LH cells to an easy released status with a lot of different size of vacuoles. On the contrary, the high dose of estrogen inhibited the storage of LH, and the LH cells were filled with secretory granules and few vacuoles. The progesterone could promote the storage of LH in an uneasy released status. The administration of estrogen progesterone combination not only inhibited the storage of LH, but also the release of LH. In this group, the LH cells containing a large amount of secretory granules and a few vacuoles showed a better uniform shape compared with those administrated with high dose of estrogen. Conclusion: Different kinds of gonadal hormones could reverse the excessive secretion of LH and recover the morphological change of LH cells to the normally physiological condition.

Beneficial effects of a decreased meal frequency on nutrient utilization,secretion of luteinizing hormones and ovarian follicular development in gilts

Background:Replacement gilts are typically fed ad libitum,whereas emerging evidence from human and rodent studies has revealed that time-restricted access to food has health benefits.The objective of this study was to investigate the effect of meal frequency on the metabolic status and ovarian follicular development in gilts.Methods:A total of 36 gilts(Landrace×Yorkshire)with an age of 150±3 d and a body weight of 77.6±3.8 kg were randomly allocated into one of three groups(n=12 in each group),and based on the group allocation,the gilts were fed at a frequency of one meal(T1),two meals(T2),or six meals per day(T6)for 14 consecutive weeks.The effects of the meal frequency on growth preference,nutrient utilization,short-chain fatty acid production by gut microbial,the post-meal dynamics in the metabolic status,reproductive hormone secretions,and ovarian follicular development in the gilts were measured.Results:The gilts in the T1 group presented a higher average daily gain(+48 g/d,P<0.05)and a higher body weight(+4.9 kg,P<0.05)than those in the T6 group.The meal frequency had no effect on the apparent digestibility of dry matter,crude protein,ether extract,ash,and gross energy,with the exception that the T1 gilts exhibited a greater NDF digestibility than the T6 gilts(P<0.05).The nitrogen balance analysis revealed that the T1 gilts presented decreased urine excretion of nitrogen(−8.17 g/d,P<0.05)and higher nitrogen retention(+9.81 g/d,P<0.05),and thus exhibited higher nitrogen utilization than the T6 gilts.The time-course dynamics of glucose,α-amino nitrogen,urea,lactate,and insulin levels in serum revealed that the T1 group exhibited higher utilization of nutrients after a meal than the T2 or T6 gilts.The T1 gilts also had a higher acetate content and SCFAs in feces than the T6 gilts(P<0.05).The age,body weight and backfat thickness of the gilts at first estrous expression were not affected by the meal frequency,but the gilts in the T1 group had higher levels of serum luteinizing hormone on the 18th day of the 3rd estrus cycle and 17β-estradiol,a larger number of growing follicles and corpora lutea,and higher mRNA expression levels of genes related to follicular development on the 19th day of the 3rd estrus cycle.Conclusions:The current findings revealed the benefits of a lower meal frequency equal feed intake on nutrient utilization and reproductive function in replacement gilts,and thus provide new insights into the nutritional strategy for replacement gilts,and the dietary pattern for other mammals,such as humans.

Immunohistochemical observation on luteinizing hormone in rat testes before and after testicular capsulotomy

Aim: In the testicular capsulotomized rats, although there was a significant increase in the luteinizing hormone (LH)levels, the secretion of testosterone remained low. In order to clarify the mechanisms of this phenomenon, the bindingof endogenous LH to the testes were observed before and after testicular capsulotomy. Methods; Peroxidase-anti-peroxidase (PAP) method was used to detect the binding of LH to the testes in rats. Results; An intense positivestaining of LH was found in the Leydig cells of both the normal and sham-operated control testes. However, at 40 d af-ter operation, the LH immunoreactivity was decreased in the Leydig cells of the capsulotomized testis. By d 60, onlyvery weak positive staining could be observed in these cells. Conclusion; A progressive reduction of endogenousLH binding to the testis occurred in the capsulotomized rat. (Asian J Androl 2001 Sep; 3 : 227 - 230)

Effect of Kisspeptin on Regulation of Growth Hormone and Luteinizing Hormone in Lactating Dairy Cows

Kisspeptin （KP） , a neuroendocrine regulator of reproduction,is hypothesized to be an integrator of metabolism and hormones critical to the regulation of reproduction. Lactation is associated with enhanced growth hormone （GH） responsiveness and reduced fertility. Our study was designed to determine the effects of lactation on KP-stimulated GH and luteinizing hormone （LH） secretion. Five non-lactating and five lactating dairy cows were used in the study. Experiments were conducted with lactating cows at weeks 1,5 and 11 after parturition. The experimental treatments （saline and KP [100 and 400 pmol / kg body weight]） were given intravenously and blood was collected and plasma was stored until later assay to determine concentrations of GH,LH,progesterone and nonesterified fatty acids. We found that neither dose of KP stimulated an increase in GH secretion. The low dose ofKP increased （P 〈0. 05） LH concentrations only in lactating cows. The higher dose of KP elicited an increase in circulating LH concentrations in both lactating and non-lactating cows. The lower dose of KP increased （P 〈 0. 05） the area under the curve for LH only in cows during week 5 of lactation,and the area under the curve of LH following the highest dose of KP was greater （P 〈 0. 05） in cows during week 5 of lactation than that for the other groups of cows. In summary,lactation status and stage of lactation did not change the sensitivity of the GH system to KP. However,an effect of stage of lactation on KP-stimulated LH secretion was detected in the dairy cows. Study of the KP system during lactation in dairy cows may provide critical insights into the mechanisms for lactation-associated changes in the reproductive axis.

基础黄体生成素和不同LH/FSH比值水平对PCOS患者辅助生殖妊娠结局的影响

目的:探究基础黄体生成素(bLH)及不同黄体生成素/卵泡刺激素(LH/FSH)比值水平对多囊卵巢综合征(PCOS)患者体外受精或卵胞浆内单精子注射(IVF/ICSI)妊娠结局的影响。方法:选择2013年7月至2020年1月在南京医科大学第一附属医院生殖医学科首次行促性腺激素释放激素(GnRH)拮抗剂灵活方案促排卵,接受IVF/ICSI治疗的424例PCOS患者的临床资料进行回顾性分析。按照bLH水平不同分为正常bLH组(LH≤10 U/L,316例)和高bLH组(LH>10 U/L,108例);同时根据LH/FSH比值水平不同分为低比值组(LH/FSH≤1,227例)、中比值组(1<LH/FSH<2,142例)、高比值组(LH/FSH≥2,55例)。分析比较各组患者一般情况、促排卵情况及妊娠结局的差异,并采用二元Logistic回归分析影响活产的相关因素。结果:①高bLH组基础FSH和抗苗勒管激素(AMH)较正常bLH组高,差异有统计学意义(P<0.05)。在促排卵情况方面,高bLH组使用拮抗剂总天数长于正常组,但获卵数和绒促性素(HCG)日子宫内膜厚度小于正常bLH组,差异有统计学意义(P<0.05)。两组患者妊娠结局相关指标比较,差异无统计学意义(P>0.05)。②不同LH/FSH比值组患者比较,高比值组AMH和窦卵泡数(AFC)高于其他两组,差异有统计学意义(P<0.05)。在促排卵情况方面,高比值组获卵数、移植胚胎数、HCG日子宫内膜厚度均低于其他两组,差异有统计学意义(P<0.05)。3组患者妊娠结局相关指标比较,差异无统计学意义(P>0.05)。③二元Logistic回归分析显示活产与bLH、LH/FSH比值无关(P>0.05),与移植胚胎数有关(P<0.05)。结论:bLH水平和LH/FSH值升高可能会影响PCOS患者促排卵结果(如获卵数和HCG日子宫内膜厚度等),但与辅助生殖的活产率未见显著相关性。

Plasma levels of luteinizing hormone and prolactin in relation to double brooding in Great Tit(Parus major)

The reproductive behaviors of birds are mainly controlled by the hypothalamus-pituitary-gonad axis.Many studies have shown that reproductive hormones are tightly linked to the breeding sub-stages.However,only a few studies have examined the temporal trend of hormone levels among different reproductive stages in multiple brooded species.We investigated the changes in plasma luteinizing hormone(LH)and prolactin(PRL)concentrations during different reproductive stages of the facultative double-brooded Great Tit(Parus major).We found that the concentrations of LH and PRL in females were significantly higher than those in males.Females had significantly higher LH and lower PRL concentrations in the pre-breeding period than in the first/second brooding periods,and there were no significant changes between the first and second brooding periods.The concentrations of LH and PRL in males had no significant difference between the pre-breeding period and the first brooding periods,while LH and PRL concentrations in the second brooding period were significantly higher than those in the first brooding period.We conclude that there are sex-based differences between LH and PRL at different stages of reproduction.The changes in LH and PRL in both males and females should be related to their physiological functions.Especially for males,individuals with higher levels of LH and PRL are more likely to maintain second clutches.

不同繁殖力新疆山羊发情周期血清FSH和LH浓度变化规律研究

为了探究不同繁殖力母羊发情周期血清中促卵泡激素(FSH)和促黄体激素(LH)分泌变化规律,试验选择新疆山羊高繁殖力母羊17只和低繁殖力母羊14只,采用酶联免疫分析法测定母羊发情周期血清中FSH和LH浓度。结果表明:高、低繁殖力母羊发情周期中FSH和LH浓度均呈脉冲波动式分泌,高繁殖力母羊血清中FSH浓度在发情周期第1,5,13,21天出现4个波峰,LH浓度在发情周期第2,5,11,21天出现4次波峰;低繁殖力母羊血清中FSH浓度在发情周期第1,5,21天出现3个波峰,LH浓度在发情周期第5,13,21天出现3次波峰。在发情期和发情前期母羊血清中FSH和LH浓度均高于发情后期和间情期,高、低繁殖力母羊血清中FSH和LH浓度均在发情前期(>17~21 d)开始升高,在发情期(0~48 h)当天达到峰值之后呈波动式下降趋势,且高繁殖力母羊发情周期4个阶段血清中FSH和LH浓度均高于低繁殖力母羊。高、低繁殖力母羊血清中FSH和LH基础浓度均在发情期最高,间情期最低。说明不同繁殖力新疆山羊母羊发情周期中FSH和LH分泌变化规律一致,高繁殖力母羊在整个发情周期中FSH和LH浓度高于低繁殖力母羊且波峰数量多于低繁殖力母羊。

重组人生长激素辅助治疗对高龄不孕IVF⁃ET患者血清FSH、E_(2)及LH水平的影响

目的探讨在实施体外受精⁃胚胎移植(IVF⁃ET)治疗过程中使用重组人生长激素(rhGH)辅助治疗对高龄不孕症女性患者血清卵泡刺激素(FSH)、雌二醇(E_(2))及促黄体生成素(LH)水平的影响。方法回顾性选取2021年1月至2023年8月邯郸市中心医院收治的高龄不孕女性102例,依据治疗方案分为对照组[予促性腺激素释放激素(GnRH)拮抗剂方案治疗,n=50]、试验组(予GnRH拮抗剂方案+rhGH辅助治疗方案,n=52);比较两组促排卵结果、性激素水平(FSH、E_(2)、LH)、妊娠结局及不良反应情况。结果试验组Gn总用量、Gn使用天数比对照组少,差异有统计学意义(P<0.05);试验组获卵总个数、可移植胚胎数及优质胚胎数比对照组高,但差异无统计学意义(P>0.05)。两组治疗后的FSH、E_(2)、LH水平均下降,且试验组治疗后的FSH、E_(2)、LH水平均低于对照组,差异有统计学意义(P<0.05)。试验组治疗后取消周期率、流产率低于对照组,临床妊娠率、活产率高于对照组,但差异无统计学意义(P>0.05)。试验组、对照组不良反应发生率比较差异无统计学意义(P>0.05)。结论相较于GnRH拮抗剂方案单独治疗,GnRH拮抗剂+rhGH辅助治疗方案可进一步调节高龄不孕女性FSH、LH、E_(2)水平,提高获卵数、优胚率及临床妊娠率。

血清LH、IGF-ⅠSDS、IGF-bp3诊断女童快速进展型中枢性性早熟的价值研究

目的分析血清黄体生成素(LH)、胰岛素样生长因子Ⅰ标准差积分(IGF-ⅠSDS)及胰岛素样生长因子结合蛋白3(IGF-bp3)在早期识别快速进展型中枢性性早熟(RP-CPP)和缓慢进展型中枢性性早熟(SP-CPP)患儿中的有效性,为临床进一步提高对女童RP-CPP的诊疗水平提供参考资料。方法回顾性分析2021年4月至2023年6月西安交通大学附属儿童医院收治的120例中枢性性早熟(CPP)女童患儿的临床资料,将其纳入研究组,按照线性生长或骨龄成熟加速等条件,将其分为RP-CPP组(71例),SP-CPP组(49例);纳入同期在院体检的青春期发育正常女童64例为对照组。比较不同人群LH、IGF-ⅠSDS及IGF-bp3水平的变化情况,采用多元Logistic回归分析影响儿童RP-CPP的相关危险因素,采用受试者工作特征(ROC)曲线评估LH、IGF-ⅠSDS及IGF-bp3预测RP-CPP的效能。结果研究组的LH、IGF-ⅠSDS、IGF-bp3及骨龄均明显高于对照组,经比较差异均有统计学意义(t值分别为26.256、47.930、20.390、14.008,P<0.05)。LH、IGF-ⅠSDS、IGF-bp3与骨龄均呈正相关(r值分别为0.435、0.502、0.397,P<0.05)。RP-CPP组的LH、IGF-ⅠSDS水平均明显高于SP-CPP组,经比较差异均有统计学意义(t值分别为7.867、33.943,P<0.05),但RP-CPP组与SP-CPP组的IGF-bp3水平比较,差异无统计学意义(P>0.05)。经多元Logistic回归分析显示,LH[OR(95%CI):1.962(1.172~3.285)]、IGF-ⅠSDS[OR(95%CI):1.707(1.142~2.552)]及IGF-bp3[OR(95%CI):2.314(1.221~4.384)]均是影响RP-CPP发生的危险因素(P<0.01)。经ROC曲线分析显示,LH、IGF-ⅠSDS及IGF-bp3单独预测RP-CPP的曲线下面积(AUC)从高到低依次为0.799、0.762、0.714,三者联合预测RP-CPP的AUC为0.864,其明显高于各指标单独预测的结果(P<0.001)。结论CPP女童的血清LH、IGF-ⅠSDS及IGF-bp3水平均存在异常,通过检测患儿的上述指标变化,可有效地预测病情的进展,LH、IGF-ⅠSDS及IGF-bp3水平较高者发生RP-CPP的风险相对较大。

醋酸亮丙瑞林联合腹腔镜子宫肌瘤剔除术对子宫肌瘤FSH、E_(2)及LH水平的影响

目的对子宫肌瘤患者实施醋酸亮瑞林、腹腔镜子宫肌瘤剔除术(LSM)联合方案,观察该联合方案对卵泡刺激素(FSH)、雌二醇(E_(2))、促黄体生成素(LH)水平的影响。方法选取100例子宫肌瘤患者,根据治疗方案的个体差异性分为对照组(LSM治疗)、观察组(醋酸亮丙瑞林+LSM治疗),各50例。比较两组血清性激素水平、术后月经指标及围术期相关治疗指标。结果治疗前,对照组血清FSH、E_(2)、LH水平分别为(27.57±3.47)U/L、(275.39±33.48)pmol/L、(20.91±2.51)U/L,观察组分别为(27.61±3.36)U/L、(276.82±33.51)pmol/L、(20.85±2.49)U/L;治疗后,对照组血清FSH、E_(2)、LH水平分别为(18.17±2.84)U/L、(216.17±26.57)pmol/L、(17.49±1.85)U/L,观察组分别为(13.05±2.29)U/L、(139.84±18.79)pmol/L、(11.67±1.36)U/L。与治疗前相比,两组血清FSH、E_(2)、LH水平均明显下降,组内差异有统计学意义(P<0.05);观察组治疗后血清FSH、E_(2)、LH水平均低于对照组,组间差异有统计学意义(P<0.05)。观察组术后月经周期时间(29.56±1.32)d短于对照组的(33.13±2.29)d,月经量(41.33±4.32)ml少于对照组的(55.45±6.69)ml,组间差异有统计学意义(P<0.05)。观察组术中出血量、盆腔引流量少于对照组,下床活动时间、住院时间短于对照组,疾病复发率低于对照组,组间差异有统计学意义(P<0.05)。结论醋酸亮丙瑞林联合LSM运用于子宫肌瘤临床治疗过程中,可明显降低患者FSE、E_(2)、LH水平,减少术中出血量与盆腔引流量,加快术后身体恢复,降低疾病复发率,值得推荐。

血清ox-LDL、LH/FSH水平与多囊卵巢综合征不孕症患者治疗结局的关系

目的分析血清氧化型低密度脂蛋白(ox-LDL)、促黄体生成素/卵泡刺激素(LH/FSH)水平与多囊卵巢综合征(PCOS)不孕症患者治疗结局的关系。方法选取PCOS不孕症患者160例(PCOS不孕症组),和同期健康女性80例(对照组),根据治疗结局将PCOS不孕症患者分为结局不良组(99例)和结局良好组(61例)。采用酶联免疫吸附法检测ox-LDL水平和计算LH/FSH。以PCOS不孕症患者治疗结局为因变量,多因素非条件Logistic回归分析其影响因素。绘制受试者工作特征(ROC)曲线评价血清ox-LDL、LH/FSH水平对PCOS不孕症患者治疗结局不良的预测效能。结果PCOS不孕症组血清ox-LDL、LH/FSH水平高于对照组(均P<0.05)。160例PCOS不孕症患者治疗结局不良率61.88%(99/160)。结局不良组血清ox-LDL、LH/FSH水平高于结局良好组(P均<0.05)。PCOS不孕症患者治疗结局不良的独立危险因素为稳态模型评估-胰岛素抵抗、ox-LDL、LH/FSH(P<0.05)。血清ox-LDL、LH/FSH水平联合预测PCOS不孕症患者治疗结局不良的曲线下面积大于血清ox-LDL、LH/FSH水平单独预测(均P<0.05)。结论血清ox-LDL、LH/FSH水平升高与PCOS不孕症患者治疗结局不良密切相关,二者联合对其有较高的预测效能。

多囊卵巢综合征患者血清AMH,LH和FSH水平检测及临床应用研究

目的分析抗缪勒氏管激素(AMH)、黄体生成素(LH)和卵泡刺激素(FSH)在常见妇科疾病患者血清中的表达水平,探讨AMH对多囊卵巢综合征(PCOS)的诊断价值。方法采用化学发光法检测1089例常见妇科疾病患者血清AMH,LH和FSH,比较不同年龄组和不同疾病组中的血清AMH水平差异,分析PCOS患者中AMH与LH/FSH的相关性,绘制ROC曲线,计算出诊断PCOS的最佳截断值。结果①AMH水平随着年龄的增加而下降;各个年龄组PCOS患者的血清AMH水平均明显高于同年龄组的健康女性(P<0.05),其他妇科疾病患者的血清AMH水平与同年龄组的健康女性相比,差异无统计学意义(P>0.05);②PCOS患者和健康女性的血清AMH水平与LH/FSH呈中度正相关(r=0.304,0.464,P<0.00);③AMH=6.895ng/ml为PCOS诊断界值时,敏感度为82.8%,特异度为79.6%,曲线下面积为0.886,具有最高诊断效能。在LH/FSH=0.960时,敏感度为75.5%,特异度为80.2%,ROC曲线下面积为0.832,具有最高诊断效能。结论血清AMH水平升高是PCOS患者生殖内分泌代谢紊乱的重要特征,采用ROC曲线分析确立AMH的cutoff值为6.895ng/ml时,对PCOS诊断具有较大临床价值。

更年宁心胶囊对阴虚内热型更年期综合征患者FSH、LH、E_2的影响及疗效

目的 :观察更年宁心胶囊对女性阴虚内热型更年期综合征患者FSH、LH、E2 和临床症状的作用。方法 :采用随机数字表法将 10 5例阴虚内热型女性更年期综合征患者按 2∶1比例随机分入治疗组 70例和对照组 35例 ,分别采用更年宁心胶囊和更年安片治疗 ,疗程 30天。观察治疗后患者FSH、LH、E2 的变化及更年期症状积分值的改善情况。结果 :治疗组治疗后血清E2 水平明显升高 ,LH明显降低 ,且优于对照组 (P <0 .0 1) ;治疗组治疗后症状积分值明显改善并优于对照组 (P <0 .0 1)。结论 :更年宁心胶囊能显著改善阴虚内热型女性更年期综合征患者的血清E2 水平和临床症状 ,临床总疗效明显优于更年安片。

β-酪啡肽对公山羊血清FSH、LH和T水平的影响

给公山羊瘤胃灌注β-酪啡肽 (β-CM) ,在灌注前后采取外周血样 ,用放射免疫分析法测定血清促卵泡素 ( FSH)、促黄体生成素 ( LH)和睾酮 ( T)水平 ,旨在研究β-酪啡肽对公山羊生殖内分泌的影响。结果表明 ,瘤胃灌注β-CM后 ,公山羊血清 LH和 T水平明显降低 ( P <0 .0 1或 P <0 .0 5) ,且此效应可被阿片受体拮抗剂纳络酮 ( naloxon)逆转 ,而β-CM灌注 ,对公山羊血清 FSH水平未见明显影响 ( P >0 .0 5)