T cell activation and proliferation via CD3-TCR complex were investigated by lymphocyte DNA synthesis in vitro.Several interfering factors were also discussed.The result indicated that lymphocyte activation and prolif...T cell activation and proliferation via CD3-TCR complex were investigated by lymphocyte DNA synthesis in vitro.Several interfering factors were also discussed.The result indicated that lymphocyte activation and proliferation are calciumdependent.A rise of cytoplasmic free Ca2+ quickly following activation with CD3 McAb is mainly due to intracellular mobilization of Ca2+,while lymphocyte proliferation needs both intracellular mobilization of Ca2+ as well as influx of extracellular Ca2+, It was confirmed that CTX sensitive G protein plays a role in regulating T cell proliferation by pretreatment with CTX suppressing lymphocyte H-TdR incorporation obviously.PLC and PKC inhibitor neomycin and P.S.S could also decrease T cell proliferation.展开更多
Objective: Despite the existence of several therapeutic strategies, the management of cervical cancer remains challenging. Our region has very little data on the interaction between the immune system and the clinical ...Objective: Despite the existence of several therapeutic strategies, the management of cervical cancer remains challenging. Our region has very little data on the interaction between the immune system and the clinical response to chemotherapy. This work examines plasma levels of galectin-3 (Gal-3) and percentages of activated T cells in patients with cervical cancer treated with chemotherapy and investigates if there is a relationship between the rates of these two elements. Methods: We compared data from 37 patients with cervical cancer undergoing chemotherapy and 42 controls with normal cervical cytology. Plasma Gal-3 concentrations were assessed by ELISA and expression of activation markers by T cells (CD69 and HLA-DR) was assessed by flow cytometry at three different time points during chemotherapy. Results: Our results showed that patients had a significantly higher concentration of Gal-3 compared to controls (4.025 vs. 1.340, p 0.001), similarly, they had a significantly high percentage of activated lymphocytes (2.610 vs. 0.731;p 0.0001). According to the response to treatment, patients with no response to treatment had a lower concentration of circulating Gal-3 but had approximately the same percentage of activated CD4 and CD8 lymphocytes as patients with a partial or total response. In addition, we found a positive correlation between the Gal-3 level and CD4 T cells expressing the activation marker CD69 (p 0.05;rho = 0.44). Conclusion: In conclusion, our results show that there would be a relationship between circulating galectin-3 and the percentage of peripheral CD4+</sup>CD69+</sup> cells in cervical cancer.展开更多
目的:观察类风湿关节炎(rheumatoid arthritis,RA)患者血清Ⅱ型胶原C端肽(typeⅡcollagen C-terminal peptide,CTX-Ⅱ)、组蛋白去乙酰化酶3(histone deacetylase 3,HDAC3)水平变化,分析其与免疫功能、疾病活动度的关系。方法:选取本院...目的:观察类风湿关节炎(rheumatoid arthritis,RA)患者血清Ⅱ型胶原C端肽(typeⅡcollagen C-terminal peptide,CTX-Ⅱ)、组蛋白去乙酰化酶3(histone deacetylase 3,HDAC3)水平变化,分析其与免疫功能、疾病活动度的关系。方法:选取本院收治的84例RA患者(RA组)和84例体检健康者(NC组)。分析血清CTX-Ⅱ、HDAC3(酶联免疫吸附法)及免疫功能指标[免疫球蛋白(immunoglobulin,Ig)A、IgG、IgM、CD3^(+)、CD4^(+)、CD8^(+)T细胞比例]、疾病活动度相关指标[C反应蛋白(C-reactive protein,CRP)、血沉(erythrocyte sedimentation rate,ESR)]水平变化情况。Pearson法分析血清CTX-Ⅱ、HDAC3分别与免疫指标、疾病活动度相关指标的相关性。结果:RA组CTX-Ⅱ(9.26±1.84 vs 4.59±0.62)、HDAC3(83.72±13.51 vs 20.15±4.90)水平高于NC组(t=22.044、40.542,P<0.05)。缓解组CTX-Ⅱ(7.37±1.71 vs 10.75±1.94)、HDAC3(72.97±12.10 vs 92.18±14.62)水平低于活动组(t=8.347、7.940,P<0.05)。RA组CD3^(+)(64.42±6.91 vs 70.42±7.34)、CD4^(+)(34.80±5.96 vs 39.74±5.80)T细胞比例低于NC组,而CD8^(+)T细胞(31.68±6.15 vs 29.62±7.15)、IgM(2.06±0.39 vs 1.47±0.30)、IgA(2.96±0.50 vs 1.51±0.36)、IgG(15.49±3.05 vs 11.83±2.19)、ESR(28.32±7.60 vs 13.75±2.18)、CRP(10.94±2.02 vs 6.08±0.73)水平显著高于NC组(P<0.05)。活动组患者CD3^(+)(62.94±6.60 vs 66.30±7.30)、CD4^(+)T(33.57±5.74 vs 36.36±6.24)细胞低于缓解组,IgM(2.19±0.41 vs 1.89±0.36)、IgA(3.08±0.54 vs 2.81±0.45)、IgG(16.16±3.27 vs 14.64±2.77)、ESR(30.06±8.15 vs 26.11±6.90)、CRP(12.37±2.24 vs 9.12±1.74)水平高于缓解组(P<0.05)。相关性分析显示,血清CTX-Ⅱ与ESR、CRP、28个关节疾病活动(disease activity score in 28 joints,DAS28)呈正相关(r=0.572、0.495、0.809,P均=0.000);血清HDAC3分别与CD3^(+)、CD4^(+)T细胞比例存在负相关(r=-0.529、-0.464,P均=0.000),而与IgM、IgA、IgG、ESR、CRP、DAS28评分呈正相关(r=0.460、0.491、0.445、0.540、0.519、0.864,P均<0.05)。结论:类风湿关节炎患者血清CTX-Ⅱ、HDAC3水平均高于体检健康者,CTX-Ⅱ与RA疾病活动度有关,HDAC3与RA免疫功能紊乱以及疾病活动度有关。展开更多
文摘T cell activation and proliferation via CD3-TCR complex were investigated by lymphocyte DNA synthesis in vitro.Several interfering factors were also discussed.The result indicated that lymphocyte activation and proliferation are calciumdependent.A rise of cytoplasmic free Ca2+ quickly following activation with CD3 McAb is mainly due to intracellular mobilization of Ca2+,while lymphocyte proliferation needs both intracellular mobilization of Ca2+ as well as influx of extracellular Ca2+, It was confirmed that CTX sensitive G protein plays a role in regulating T cell proliferation by pretreatment with CTX suppressing lymphocyte H-TdR incorporation obviously.PLC and PKC inhibitor neomycin and P.S.S could also decrease T cell proliferation.
文摘Objective: Despite the existence of several therapeutic strategies, the management of cervical cancer remains challenging. Our region has very little data on the interaction between the immune system and the clinical response to chemotherapy. This work examines plasma levels of galectin-3 (Gal-3) and percentages of activated T cells in patients with cervical cancer treated with chemotherapy and investigates if there is a relationship between the rates of these two elements. Methods: We compared data from 37 patients with cervical cancer undergoing chemotherapy and 42 controls with normal cervical cytology. Plasma Gal-3 concentrations were assessed by ELISA and expression of activation markers by T cells (CD69 and HLA-DR) was assessed by flow cytometry at three different time points during chemotherapy. Results: Our results showed that patients had a significantly higher concentration of Gal-3 compared to controls (4.025 vs. 1.340, p 0.001), similarly, they had a significantly high percentage of activated lymphocytes (2.610 vs. 0.731;p 0.0001). According to the response to treatment, patients with no response to treatment had a lower concentration of circulating Gal-3 but had approximately the same percentage of activated CD4 and CD8 lymphocytes as patients with a partial or total response. In addition, we found a positive correlation between the Gal-3 level and CD4 T cells expressing the activation marker CD69 (p 0.05;rho = 0.44). Conclusion: In conclusion, our results show that there would be a relationship between circulating galectin-3 and the percentage of peripheral CD4+</sup>CD69+</sup> cells in cervical cancer.
文摘目的:观察类风湿关节炎(rheumatoid arthritis,RA)患者血清Ⅱ型胶原C端肽(typeⅡcollagen C-terminal peptide,CTX-Ⅱ)、组蛋白去乙酰化酶3(histone deacetylase 3,HDAC3)水平变化,分析其与免疫功能、疾病活动度的关系。方法:选取本院收治的84例RA患者(RA组)和84例体检健康者(NC组)。分析血清CTX-Ⅱ、HDAC3(酶联免疫吸附法)及免疫功能指标[免疫球蛋白(immunoglobulin,Ig)A、IgG、IgM、CD3^(+)、CD4^(+)、CD8^(+)T细胞比例]、疾病活动度相关指标[C反应蛋白(C-reactive protein,CRP)、血沉(erythrocyte sedimentation rate,ESR)]水平变化情况。Pearson法分析血清CTX-Ⅱ、HDAC3分别与免疫指标、疾病活动度相关指标的相关性。结果:RA组CTX-Ⅱ(9.26±1.84 vs 4.59±0.62)、HDAC3(83.72±13.51 vs 20.15±4.90)水平高于NC组(t=22.044、40.542,P<0.05)。缓解组CTX-Ⅱ(7.37±1.71 vs 10.75±1.94)、HDAC3(72.97±12.10 vs 92.18±14.62)水平低于活动组(t=8.347、7.940,P<0.05)。RA组CD3^(+)(64.42±6.91 vs 70.42±7.34)、CD4^(+)(34.80±5.96 vs 39.74±5.80)T细胞比例低于NC组,而CD8^(+)T细胞(31.68±6.15 vs 29.62±7.15)、IgM(2.06±0.39 vs 1.47±0.30)、IgA(2.96±0.50 vs 1.51±0.36)、IgG(15.49±3.05 vs 11.83±2.19)、ESR(28.32±7.60 vs 13.75±2.18)、CRP(10.94±2.02 vs 6.08±0.73)水平显著高于NC组(P<0.05)。活动组患者CD3^(+)(62.94±6.60 vs 66.30±7.30)、CD4^(+)T(33.57±5.74 vs 36.36±6.24)细胞低于缓解组,IgM(2.19±0.41 vs 1.89±0.36)、IgA(3.08±0.54 vs 2.81±0.45)、IgG(16.16±3.27 vs 14.64±2.77)、ESR(30.06±8.15 vs 26.11±6.90)、CRP(12.37±2.24 vs 9.12±1.74)水平高于缓解组(P<0.05)。相关性分析显示,血清CTX-Ⅱ与ESR、CRP、28个关节疾病活动(disease activity score in 28 joints,DAS28)呈正相关(r=0.572、0.495、0.809,P均=0.000);血清HDAC3分别与CD3^(+)、CD4^(+)T细胞比例存在负相关(r=-0.529、-0.464,P均=0.000),而与IgM、IgA、IgG、ESR、CRP、DAS28评分呈正相关(r=0.460、0.491、0.445、0.540、0.519、0.864,P均<0.05)。结论:类风湿关节炎患者血清CTX-Ⅱ、HDAC3水平均高于体检健康者,CTX-Ⅱ与RA疾病活动度有关,HDAC3与RA免疫功能紊乱以及疾病活动度有关。
