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Macamide B促进缺氧缺血性脑损伤新生小鼠神经元的PGRN表达
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作者 周倩 李莉霞 +5 位作者 杨晓夏 杨俊华 文锦坤 李朋飞 罗利 李国营 《中国临床解剖学杂志》 CSCD 北大核心 2023年第3期304-309,共6页
目的研究玛咖酰胺B(Macamide B)对缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)的新生小鼠神经元中颗粒蛋白前体(Progranulin,PGRN)及糖氧剥夺(oxygen-glucose deprivation,OGD)后的小鼠海马神经元细胞系(HT22)中PGRN表达的影... 目的研究玛咖酰胺B(Macamide B)对缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)的新生小鼠神经元中颗粒蛋白前体(Progranulin,PGRN)及糖氧剥夺(oxygen-glucose deprivation,OGD)后的小鼠海马神经元细胞系(HT22)中PGRN表达的影响,探究Macamide B在新生小鼠HIBD后发挥神经保护作用的机制。方法建立HIBD小鼠模型,将出生后7 d的C57小鼠随机分为Sham组、HIBD组和Macamide B组;HT22细胞培养并制备OGD细胞模型,分为Control组、OGD组和Macamide B组。通过免疫荧光、Western blot检测小鼠神经元或HT22细胞中PGRN的表达。结果体内实验显示,与Sham组相比,HIBD组小鼠神经元中PGRN表达降低;与HIBD组相比,Macamide B组小鼠神经元中PGRN表达升高。在体外,与Control组相比,OGD组HT22细胞中PGRN表达降低;与OGD组相比,Macamide B组HT22细胞中PGRN表达显著升高。结论Macamide B可能通过促进PGRN表达对HIBD新生小鼠发挥神经保护作用。 展开更多
关键词 缺氧缺血性脑损伤 Macamide B 神经保护 PGRN
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Nutritional Compositions of Maca Grown in Ebian and HPLC Fingerprints of Macaene and Macamide
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作者 陈建 夏陈 +1 位作者 朱永清 栢凤女 《Agricultural Science & Technology》 CAS 2016年第4期947-950,共4页
In the present study, the nutritional compositions of maca which was grown in a mountain area at an elevation of 2 200-2 800 m of Ebian County,Sichuan Province were measured, and then HPLC analysis on two representati... In the present study, the nutritional compositions of maca which was grown in a mountain area at an elevation of 2 200-2 800 m of Ebian County,Sichuan Province were measured, and then HPLC analysis on two representative active compounds(macaene and macamide) in the maca sample was performed.The results revealed that there were 24.20% total protein, 18.40% total amino acids(including 3.84% arginine), 42.80% total sugars, 1.36% fat and kinds of minerals(including 1.14% potassium) in Ebian maca. HPLC fingerprints of macaene and macamide of Ebian maca were similar to those of Peru maca. The results suggested that maca could be cultivated with good quality in some mountain areas with an altitude1 000 m lower than the origin place in Peru. 展开更多
关键词 MACA Nutritional compositions Macaene Macamide HPLC fingerprints
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