BACKGROUND Macro-aspartate aminotransferase(AST), a macroenzyme, is a high-molecular mass complex formed by self-polymerization or association with other serum components that are difficult for the kidney to clear, le...BACKGROUND Macro-aspartate aminotransferase(AST), a macroenzyme, is a high-molecular mass complex formed by self-polymerization or association with other serum components that are difficult for the kidney to clear, leading to the isolated elevation of serum AST activity. Cases of macro-AST formation are rare, with only 3 published in the English language literature up to September 2019 in China. In this paper, we present a case in which an asymptomatic woman with persistent isolated elevated AST was confirmed as having macro-AST by the polyethylene glycol precipitation method.CASE SUMMARY A 34-year-old woman was referred to our clinic for elevated AST levels with normal levels of other liver-associated enzymes on November 12, 2018. Her AST level of liver function test had been abnormal for 7 mo before she came to the clinic. The patient was asymptomatic with a normal physical examination. There was no relevant family history and no alcohol consumption or smoking. She had a several-month history of traditional Chinese medical taking and had stopped it 1 year prior. The laboratory tests in our clinic showed only the elevation of AST(89.5 U/L) with no other significant abnormalities. We performed the precipitation technique with polyethylene glycol to confirm the presence of macro-AST. Then for almost a year, her AST level still fluctuated in the abnormal range.CONCLUSION This case highlights that clinical physicians should be familiar with this rare condition of persistent isolated AST elevation due to the presence of macro-AST to avoid unnecessary investigation and patient anxiety.展开更多
An extended displacement discontinuity method (EDDM) is proposed to analyze the stress wave propagation in jointed viscoelastic rock mass (VRM).The discontinuities in a rock mass are divided into two groups.The primar...An extended displacement discontinuity method (EDDM) is proposed to analyze the stress wave propagation in jointed viscoelastic rock mass (VRM).The discontinuities in a rock mass are divided into two groups.The primary group with an average geometrical size larger than or in the same order of magnitude of wavelength of a concerned stress wave is defined as 'macro-joints',while the secondary group with a high density and relatively small geometrical size compared to the wavelength is known as 'micro-defects'.The rock mass with micro-defects is modeled as an equivalent viscoelastic medium while the macro-joints in the rock mass are modeled explicitly as physical discontinuities.Viscoelastic properties of a micro-defected sedimentary rock are obtained by longitudinally impacting a cored long sedimentary rod with a pendulum.Wave propagation coefficient and dynamic viscoelastic modulus are measured.The EDDM is then successfully employed to analyze the wave propagation across macro-joint in VRM.The effect of the rock viscosity on the stress wave propagation is evaluated by comparing the results of VRM from the presented EDDM with those of an elastic rock mass (ERM) from the conventional displacement discontinuity method (CDDM).The CDDM is a special case of the EDDM under the condition that the rock viscosity is ignored.Comparison of the reflected and transmitted waves shows that the essential rock viscosity has a significant effect on stress wave attenuation.When a short propagation distance of a stress wave is considered,the results obtained from the CDDM approximate to the EDDM solutions,however,when the propagation distance is sufficiently long relative to the wavelength,the effect of rock viscosity on the stress wave propagation cannot be ignored.展开更多
剪切型断裂是岩土工程中常见的破坏模式,了解剪切破坏机理并准确预测剪切型裂纹的萌生、扩展过程对保障工程结构的安全性与稳定性具有重要意义.文章建立了基于比例边界有限元法(scaled boundary finite element methods,SBFEM)和非局部...剪切型断裂是岩土工程中常见的破坏模式,了解剪切破坏机理并准确预测剪切型裂纹的萌生、扩展过程对保障工程结构的安全性与稳定性具有重要意义.文章建立了基于比例边界有限元法(scaled boundary finite element methods,SBFEM)和非局部宏-微观损伤模型的剪切型裂纹动态开裂模拟方法,定义了基于偏应变概念的物质点对的正伸长量,可作为预测剪切型裂纹扩展行为的动态开裂准则,一点的损伤定义为该点影响域范围内连接的物质键损伤的加权平均值,而物质键的损伤则与基于偏应变概念的物质点对的正伸长量相关联,并引入能量退化函数建立结构域几何拓扑损伤与能量损失之间的关系,将拓扑损伤与应力应变联系起来,通过能量退化函数修正了SBFEM的刚度系数矩阵,得到了子域在损伤状态下的刚度矩阵,推导了考虑结构损伤的SBFEM动力控制方程,采用Newmark隐式算法对控制方程进行时间离散.最后,通过3个典型算例验证了建议的模型可较好地模拟剪切型断裂问题,能够很好地捕捉剪切型裂纹的扩展路径,并得到较为准确的载荷-位移曲线.展开更多
论文建立了基于比例边界有限元法(scaled boundary finite element methods,SBFEM)框架的非局部宏微观损伤模型,考虑材料细观物理参数的空间变异性,探讨了材料参数的空间变异性对结构开裂过程的影响。结果表明:考虑材料参数空间变异性后...论文建立了基于比例边界有限元法(scaled boundary finite element methods,SBFEM)框架的非局部宏微观损伤模型,考虑材料细观物理参数的空间变异性,探讨了材料参数的空间变异性对结构开裂过程的影响。结果表明:考虑材料参数空间变异性后,裂纹扩展路径具有不确定性,建议的模型能够很好地反应材料内在的随机性;随着结构受力情况的复杂化和结构本体缺陷的增多,裂纹开裂模式的变异性也会增大。自相关长度和参数变异系数对结构开裂分析结果有重要影响。展开更多
对磷酸香草醛显色法的反应条件进行优化研究,建立了磷酸香草醛显色法快速检测微生物胞内油脂的方法,并探讨该方法在微生物油脂测定上的应用。经过实验确定最佳比色条件为:将油脂样品蒸干溶剂后加入2 mL 98%硫酸,于90℃水浴20 min,冷却...对磷酸香草醛显色法的反应条件进行优化研究,建立了磷酸香草醛显色法快速检测微生物胞内油脂的方法,并探讨该方法在微生物油脂测定上的应用。经过实验确定最佳比色条件为:将油脂样品蒸干溶剂后加入2 mL 98%硫酸,于90℃水浴20 min,冷却至室温后添加3 mL 0.25 g/L香草醛磷酸溶液(34%的磷酸溶液配制),摇匀室温显色20 min,扣除非油脂成分干扰,在530 nm波长下测定吸光度,经验证该反应体系稳定性良好。在裂殖壶菌和隐甲藻两种产DHA油脂微生物中,通过称重法与磷酸香草醛显色法比较,进一步证实建立的方法分析结果准确可靠,分析过程简便、快速,稳定性好,适合作为常规分析检测方法。展开更多
文摘BACKGROUND Macro-aspartate aminotransferase(AST), a macroenzyme, is a high-molecular mass complex formed by self-polymerization or association with other serum components that are difficult for the kidney to clear, leading to the isolated elevation of serum AST activity. Cases of macro-AST formation are rare, with only 3 published in the English language literature up to September 2019 in China. In this paper, we present a case in which an asymptomatic woman with persistent isolated elevated AST was confirmed as having macro-AST by the polyethylene glycol precipitation method.CASE SUMMARY A 34-year-old woman was referred to our clinic for elevated AST levels with normal levels of other liver-associated enzymes on November 12, 2018. Her AST level of liver function test had been abnormal for 7 mo before she came to the clinic. The patient was asymptomatic with a normal physical examination. There was no relevant family history and no alcohol consumption or smoking. She had a several-month history of traditional Chinese medical taking and had stopped it 1 year prior. The laboratory tests in our clinic showed only the elevation of AST(89.5 U/L) with no other significant abnormalities. We performed the precipitation technique with polyethylene glycol to confirm the presence of macro-AST. Then for almost a year, her AST level still fluctuated in the abnormal range.CONCLUSION This case highlights that clinical physicians should be familiar with this rare condition of persistent isolated AST elevation due to the presence of macro-AST to avoid unnecessary investigation and patient anxiety.
文摘An extended displacement discontinuity method (EDDM) is proposed to analyze the stress wave propagation in jointed viscoelastic rock mass (VRM).The discontinuities in a rock mass are divided into two groups.The primary group with an average geometrical size larger than or in the same order of magnitude of wavelength of a concerned stress wave is defined as 'macro-joints',while the secondary group with a high density and relatively small geometrical size compared to the wavelength is known as 'micro-defects'.The rock mass with micro-defects is modeled as an equivalent viscoelastic medium while the macro-joints in the rock mass are modeled explicitly as physical discontinuities.Viscoelastic properties of a micro-defected sedimentary rock are obtained by longitudinally impacting a cored long sedimentary rod with a pendulum.Wave propagation coefficient and dynamic viscoelastic modulus are measured.The EDDM is then successfully employed to analyze the wave propagation across macro-joint in VRM.The effect of the rock viscosity on the stress wave propagation is evaluated by comparing the results of VRM from the presented EDDM with those of an elastic rock mass (ERM) from the conventional displacement discontinuity method (CDDM).The CDDM is a special case of the EDDM under the condition that the rock viscosity is ignored.Comparison of the reflected and transmitted waves shows that the essential rock viscosity has a significant effect on stress wave attenuation.When a short propagation distance of a stress wave is considered,the results obtained from the CDDM approximate to the EDDM solutions,however,when the propagation distance is sufficiently long relative to the wavelength,the effect of rock viscosity on the stress wave propagation cannot be ignored.
文摘剪切型断裂是岩土工程中常见的破坏模式,了解剪切破坏机理并准确预测剪切型裂纹的萌生、扩展过程对保障工程结构的安全性与稳定性具有重要意义.文章建立了基于比例边界有限元法(scaled boundary finite element methods,SBFEM)和非局部宏-微观损伤模型的剪切型裂纹动态开裂模拟方法,定义了基于偏应变概念的物质点对的正伸长量,可作为预测剪切型裂纹扩展行为的动态开裂准则,一点的损伤定义为该点影响域范围内连接的物质键损伤的加权平均值,而物质键的损伤则与基于偏应变概念的物质点对的正伸长量相关联,并引入能量退化函数建立结构域几何拓扑损伤与能量损失之间的关系,将拓扑损伤与应力应变联系起来,通过能量退化函数修正了SBFEM的刚度系数矩阵,得到了子域在损伤状态下的刚度矩阵,推导了考虑结构损伤的SBFEM动力控制方程,采用Newmark隐式算法对控制方程进行时间离散.最后,通过3个典型算例验证了建议的模型可较好地模拟剪切型断裂问题,能够很好地捕捉剪切型裂纹的扩展路径,并得到较为准确的载荷-位移曲线.
文摘论文建立了基于比例边界有限元法(scaled boundary finite element methods,SBFEM)框架的非局部宏微观损伤模型,考虑材料细观物理参数的空间变异性,探讨了材料参数的空间变异性对结构开裂过程的影响。结果表明:考虑材料参数空间变异性后,裂纹扩展路径具有不确定性,建议的模型能够很好地反应材料内在的随机性;随着结构受力情况的复杂化和结构本体缺陷的增多,裂纹开裂模式的变异性也会增大。自相关长度和参数变异系数对结构开裂分析结果有重要影响。
文摘对磷酸香草醛显色法的反应条件进行优化研究,建立了磷酸香草醛显色法快速检测微生物胞内油脂的方法,并探讨该方法在微生物油脂测定上的应用。经过实验确定最佳比色条件为:将油脂样品蒸干溶剂后加入2 mL 98%硫酸,于90℃水浴20 min,冷却至室温后添加3 mL 0.25 g/L香草醛磷酸溶液(34%的磷酸溶液配制),摇匀室温显色20 min,扣除非油脂成分干扰,在530 nm波长下测定吸光度,经验证该反应体系稳定性良好。在裂殖壶菌和隐甲藻两种产DHA油脂微生物中,通过称重法与磷酸香草醛显色法比较,进一步证实建立的方法分析结果准确可靠,分析过程简便、快速,稳定性好,适合作为常规分析检测方法。
