NT build 443, or profile fitting method, is often used to measure the diffusion coefficient of chloride in concrete. However, this method is quite laborious and needs special equipment. Colorimetric method is a quick ...NT build 443, or profile fitting method, is often used to measure the diffusion coefficient of chloride in concrete. However, this method is quite laborious and needs special equipment. Colorimetric method is a quick and simple method to measure the penetration depth of chloride by spraying 0.1 mol/L silver nitrate solution. The objective of this work is to study the possibility of the use of colorimetric method in the calculation of non-steady-state diffusion coefficient. Twelve concrete mixtures with different supplementary cementitious materials and water-to-cement ratios of 0.35, 0.48 and 0.6 were used for study. According to NT build 443, the concrete specimens were immersed in 165 g/L NaC1 （2.8 mol/L） solution for 42 d. Both water-soluble （convert to free chloride） chloride and acid-soluble chloride at different layers of specimens were measured. The results show that the mean value of free chloride concentration at the color change boundary Cd was 0.306 mol/L. The surface free chloride concentration cs was obtained by profile fitting method, which was 40% lower than the chloride concentration of exposure solution after an immersion period of 42 d. Chloride diffusion coefficients obtained by the colorimetric method was not well correlated with those obtained by profile fitting method.展开更多
A simple, rapid and precise method has been developed for determination of lipase activity in microbial media. The method is based on using phenyl acetate as substrate for lipase and determination of liberated phenol ...A simple, rapid and precise method has been developed for determination of lipase activity in microbial media. The method is based on using phenyl acetate as substrate for lipase and determination of liberated phenol by Folin Ciocalteu reagent. Reaction mixture containing substrate 2.4 ml of phenyl acetate 165 μM in Tris HCl buffer, 0.1 M and pH 7, with 1% (v/v) Triton X-100) and 0.1 ml lipase is incubated at 40?C during 10 minutes and the absorbance was measured at 750 nm. Linearity was observed in the concentration range 0-0.8 g/L lipase.展开更多
This study aims to evaluate the presence of free chlorides by the silver nitrate colorimetric spraying method in mortars made with Portland cement CP II Z-32. In order to make an assessment beyond the qualitative anal...This study aims to evaluate the presence of free chlorides by the silver nitrate colorimetric spraying method in mortars made with Portland cement CP II Z-32. In order to make an assessment beyond the qualitative analysis, a calculation of the contaminated areas by free chlorides was performed by measuring the regions using a computer image analysis software （IMAGEJ）. The experimental part of the research involved samples of 5 cm x 10 cm of mortar and 0.1 M solution of silver nitrate in distilled water. The mortar samples were made with cement CP II Z-32 with the following concentrations of chloride incorporated into the mixing water： 0%0, 0.4%, 0.6%, 0.8%, 1.0% and 2.0%, in relation to the mass of cement, totaling 108 samples. The average of percentage results from the contaminated area of the series of samples was respectively 0%, 14.9694%, 19.7444%, 46.0239%, 62.3311% and 96.0083% in relation to the total area, concluding that the method of silver nitrate spraying is applicable and that the white color indicates the presence of chloride ions and other possible aggressive salts to the structure or the concrete.展开更多
In order to accurately and automatically measure the light emitting diode(LED) colorimetric parameters,the design of a measurement system by adopting a high-performance spectrometer and looking-up table method was pre...In order to accurately and automatically measure the light emitting diode(LED) colorimetric parameters,the design of a measurement system by adopting a high-performance spectrometer and looking-up table method was presented based on the LabVIEW.First,the data of the LED relative spectral power distribution(SPD) were read by the spectrometer to measure LED chromaticity coordinate,and the slopes table were formed by the LED chromaticity coordinate and the equal stimulus point.Then,the CIE1931 chromaticity diagram was divided into 4 different regions to ensure the slopes table that had the character of monotonic decreasing.Finally,the LED dominant wavelength and purity were automatically calculated using the LabVIEW programs.The data of LEDs' colorimetric parameters have demonstrated that the measurement method in this paper can achieve higher precision result.展开更多
A colorimetric method for the direct determination of hydrogen peroxide in aqueous solution is described. H2O2 stoichiometrically converts 4-nitrophenyl boronic acid or 4-nitrophenyl boronic acid pinacol ester into 4-...A colorimetric method for the direct determination of hydrogen peroxide in aqueous solution is described. H2O2 stoichiometrically converts 4-nitrophenyl boronic acid or 4-nitrophenyl boronic acid pinacol ester into 4-nitrophenol, which can be quantified by measuring the absorption at 400 nm in neutral or basic media. The reactions proceed fast under basic conditions and complete in 2 minutes to at pH 11 and 80?C. The linear range for the colorimetric method extends beyond 1.0 to 40 μM H2O2, and the limit of detection is ~1.0 μM H2O2. This method offers a convenient and practical process for rapid determination of hydrogen peroxide in aqueous media. Compared to many other techniques in H2O2 detection, this process is a direct measurement of H2O2, and is relatively unaffected by the presence of various salts, metal ions and the chelator EDTA.展开更多
基金Project(2010QZZD018) supported by Central South Universtiy,China,and Ghent University,Belgium
文摘NT build 443, or profile fitting method, is often used to measure the diffusion coefficient of chloride in concrete. However, this method is quite laborious and needs special equipment. Colorimetric method is a quick and simple method to measure the penetration depth of chloride by spraying 0.1 mol/L silver nitrate solution. The objective of this work is to study the possibility of the use of colorimetric method in the calculation of non-steady-state diffusion coefficient. Twelve concrete mixtures with different supplementary cementitious materials and water-to-cement ratios of 0.35, 0.48 and 0.6 were used for study. According to NT build 443, the concrete specimens were immersed in 165 g/L NaC1 （2.8 mol/L） solution for 42 d. Both water-soluble （convert to free chloride） chloride and acid-soluble chloride at different layers of specimens were measured. The results show that the mean value of free chloride concentration at the color change boundary Cd was 0.306 mol/L. The surface free chloride concentration cs was obtained by profile fitting method, which was 40% lower than the chloride concentration of exposure solution after an immersion period of 42 d. Chloride diffusion coefficients obtained by the colorimetric method was not well correlated with those obtained by profile fitting method.
文摘A simple, rapid and precise method has been developed for determination of lipase activity in microbial media. The method is based on using phenyl acetate as substrate for lipase and determination of liberated phenol by Folin Ciocalteu reagent. Reaction mixture containing substrate 2.4 ml of phenyl acetate 165 μM in Tris HCl buffer, 0.1 M and pH 7, with 1% (v/v) Triton X-100) and 0.1 ml lipase is incubated at 40?C during 10 minutes and the absorbance was measured at 750 nm. Linearity was observed in the concentration range 0-0.8 g/L lipase.
文摘This study aims to evaluate the presence of free chlorides by the silver nitrate colorimetric spraying method in mortars made with Portland cement CP II Z-32. In order to make an assessment beyond the qualitative analysis, a calculation of the contaminated areas by free chlorides was performed by measuring the regions using a computer image analysis software （IMAGEJ）. The experimental part of the research involved samples of 5 cm x 10 cm of mortar and 0.1 M solution of silver nitrate in distilled water. The mortar samples were made with cement CP II Z-32 with the following concentrations of chloride incorporated into the mixing water： 0%0, 0.4%, 0.6%, 0.8%, 1.0% and 2.0%, in relation to the mass of cement, totaling 108 samples. The average of percentage results from the contaminated area of the series of samples was respectively 0%, 14.9694%, 19.7444%, 46.0239%, 62.3311% and 96.0083% in relation to the total area, concluding that the method of silver nitrate spraying is applicable and that the white color indicates the presence of chloride ions and other possible aggressive salts to the structure or the concrete.
基金National Natural Science Foundation of China (No. 51105289)Doctoral Program Foundation of Self-determined and Innovative Research Funds of WUT,China (No. 2010-ZY-JD-034)
文摘In order to accurately and automatically measure the light emitting diode(LED) colorimetric parameters,the design of a measurement system by adopting a high-performance spectrometer and looking-up table method was presented based on the LabVIEW.First,the data of the LED relative spectral power distribution(SPD) were read by the spectrometer to measure LED chromaticity coordinate,and the slopes table were formed by the LED chromaticity coordinate and the equal stimulus point.Then,the CIE1931 chromaticity diagram was divided into 4 different regions to ensure the slopes table that had the character of monotonic decreasing.Finally,the LED dominant wavelength and purity were automatically calculated using the LabVIEW programs.The data of LEDs' colorimetric parameters have demonstrated that the measurement method in this paper can achieve higher precision result.
文摘A colorimetric method for the direct determination of hydrogen peroxide in aqueous solution is described. H2O2 stoichiometrically converts 4-nitrophenyl boronic acid or 4-nitrophenyl boronic acid pinacol ester into 4-nitrophenol, which can be quantified by measuring the absorption at 400 nm in neutral or basic media. The reactions proceed fast under basic conditions and complete in 2 minutes to at pH 11 and 80?C. The linear range for the colorimetric method extends beyond 1.0 to 40 μM H2O2, and the limit of detection is ~1.0 μM H2O2. This method offers a convenient and practical process for rapid determination of hydrogen peroxide in aqueous media. Compared to many other techniques in H2O2 detection, this process is a direct measurement of H2O2, and is relatively unaffected by the presence of various salts, metal ions and the chelator EDTA.
