Rapid hologram generation through backward ray tracing and adaptive-resolution wavefront recording plane

An advanced method for rapidly computing holograms of large three-dimensional(3D)objects combines backward ray tracing with adaptive resolution wavefront recording plane(WRP)and adaptive angular spectrum propagation.In the initial phase,a WRP with adjustable resolution and sampling interval based on the object’s size is defined to capture detailed information from large 3D objects.The second phase employs an adaptive angular spectrum method(ASM)to efficiently compute the propagation from the large-sized WRP to the small-sized computer-generated hologram(CGH).The computation process is accelerated using CUDA and OptiX.Optical experiments confirm that the algorithm can generate high-quality holograms with shadow and occlusion effects at a resolution of 1024×1024 in 29 ms.

Isolation of Protoplast and Ion Channel Recording in Plasma Membrane of Suspension Cells of Populus euphratica

The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1 0% cellulase “onozuka” R\|10, 0\^01% pectolyase Y\|23,0\^15% macerozyme R\|10 and 0\^1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell\|attached recording of patch\|clamp technique. In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species.

FAULT LOCATION AND RECORDING SYSTEM OF TRANSMISSION LINES BASED ON COMPUTER NETWORK

In this paper a fault location and recording system based on a computer network is presented. A brief description of the system structure and main features are given. Emphasis is placed on the accurate fault location method for extra high voltage and long distance transmission lines.

Microelectrode array for bioelectrical signal stimulation and recording

A microelectrode array（MEA） is presented, which is composed of 60 independent electrodes with 59 working ones and one reference one, and they are divided into 30 pairs. Except for the reference electrode, each pair consists of one stimulating electrode and one recording electrode. Supported by the peripheral circuits, four electrode states to study the bioelectrical signal of biological tissue or slice cultured in-vitro on the surface of the electrodes can be realized through each pair of electrodes. The four electrode states are stimulation, recording, stimulation and recording simultaneously, and isolation. The state of each pair of working electrodes can be arbitrarily controlled according to actual needs. The MEAs are fabricated in printed circuit board （PCB） technology. The total area of the PCB-based MEA is 49 mm × 49 mm. The impedance measurement of MEA is carried out in 0.9% sodium chloride solution at room temperature by means of 2-point measurements with an Agilent LCR meter, and the test signal for the impedance measurement is sinusoidal （AC voltage 50 mV, sweeping frequency 20 Hz to 10 kHz）. The electrode impedance is between 200 and 3 kΩ while the frequency is between 500 and 1 000 Hz. The electrode impedance magnitude is inversely proportional to the frequency. Experiments of toad sciatic nerve in-vitro stimulation and recording and signal regeneration between isolated toad sciatic nerves are carried out on the PCB-based MEA. The results show that the MEA can be used for bioelectrical signal stimulation, recording, stimulation and recording simultaneously, and isolation of biological tissues or slices in-vitro.

An optimized recording method to characterize biophysical and pharmacological properties of acid-sensing ion channel

Objective To re-confirm and characterize the biophysical and pharmacological properties of endogenously expressed human acid-sensing ion channel 1a （hASIC1a） current in HEK293 cells with a modified perfusion methods. Methods With cell floating method, which is separating the cultured cell from coverslip and putting the cell in front of perfusion tubing, whole cell patch clamp technique was used to record hASICla currents evoked by low pH external solution. Results Using cell floating method, the amplitude of hASICla currents activated by pH 5.0 in HEK293 cells is twice as large as that by the conventional method where the cells remain attached to coverslip. The time to reach peak at two different recording conditions is （21±5） ms and （270±25） ms, respectively. Inactivation time constants are （496±23） ms and （2284±120） ms, respectively. The cell floating method significantly increases the amiloride potency of block on hASIC 1 a [IC50 is （3.4± 1.1 ） μmol/L and （2.4± 0.9） μmol/L, respectively]. Both recording methods have similar pH activation ECs0 （6.6±0.6, 6.6±0.7, respectively）. Conclusion ASICs channel activation requires fast exchange of extracellular solution with the different pH values. With cell floating method, the presence of hASIC la current was re-confirmed and the biophysical and pharmacological properties of hASIC la channel in HEK293 cells was precisely characterized. This method could be used to study all ASICs and other ligand-gated channels that require fast extracellular solution exchange.

Strong motion observations and recordings from the great Wenchuan Earthquake

The National Strong Motion Observation Network System （NSMONS） of China is briefly introduced in this paper. The NSMONS consists of permanent free-field stations, special observation arrays, mobile observatories and a network management system. During the Wenchuan Earthquake, over 1,400 components of acceleration records were obtained from 460 permanent free-field stations and three arrays for topographical effect and structural response observation in the network system from the main shock, and over 20,000 components of acceleration records from strong aftershocks occurred before August 1, 2008 were also obtained by permanent free-field stations of the NSMONS and 59 mobile instruments quickly deployed after the main shock. The strong motion recordings from the main shock and strong aftershocks are summarized in this paper. In the ground motion recordings, there are over 560 components with peak ground acceleration （PGA） over 10 Gal, the largest being 957.7 Gal. The largest PGA recorded during the aftershock exceeds 300 Gal.

Improved HVSR site classification method for free-field strong motion stations validated with Wenchuan aftershock recordings

Local site conditions play an important role in the effective application of strong motion recordings.In the China National Strong Motion Observation Network System（NSMONS）,some of the stations do not provide borehole information,and correspondingly,do not assign the site classes yet.In this paper,site classification methodologies for free-field strong motion stations are reviewed and the limitations and uncertainties of the horizontal-to-vertical spectral ratio（HVSR） methods are discussed.Then,a new method for site classification based on the entropy weight theory is proposed.The proposed method avoids the head or tail joggle phenomenon by providing the objective and subjective weights.The method was applied to aftershock recordings from the 2008 Wenchuan earthquake,and 54 free-field NSMONS stations were selected for site classification and the mean HVSRs were calculated.The results show that the improved HVSR method proposed in this paper has a higher success rate and could be adopted in NSMONS.

Magnetic property and recording performance of chemical deposition CoP thin films

The thickness of CoP thin films prepared by wet chemical deposition is of crucial importance on magnetic property and recording perform-ance. The coercivity of CoP films decreased with increasing film thickness. The coercivity was 45.37 kA m 1 at the thickness of 300 nm, and decreased to 21.65 kA m 1 at 5.7 μm. Recording performance tests indicate that, for drums with the same size, different recorded magnetic pole density have different thickness requirements. For 40 mm diameter magnetic drum, the optimal CoP thickness is 3～10 μm for 256 re-corded magnetic poles, 1～2 μm for 512 recorded magnetic poles, and 500～800 nm for 1024 recorded magnetic poles.

Characteristics of response spectra for long-periods of main-shock recordings of the Chi-Chi earthquake

Current practice uses predictive models to extrapolate long-period response spectra based on far-field recordings in moderate and weak earthquakes. However, the spectra are not long enough and the data are often not reliable, which means that the seismic design code cannot accurately define seismic design requirements for long-period structures. The near-field recordings in the main-shock of the Chi-Chi earthquake have a large signal-to-noise ratio （SNR）, which makes them suitable for studying the long-period acceleration response spectrum up to 20 sec. The acceleration response spectra from 246 stations within 120 km of the causative fault are statistically analyzed in this paper. The influence of distance and site conditions on long-period response spectrum is discussed, and the shapes of the amplification spectra are compared with the standard spectra specified in the seismic design code of China. Finally, suggestions for future revisions to the code are proposed.

Multilevel read-only optical recording methods

The advantages of read-only storage is the predominance of optical recording relative to magnetic and other rewritable methods. Multilevel （ML） read-only technology has been a trend to improve the data capacity and transfer rate. Based on the principle and coding method of ML, this paper demonstrates some ML read-only recording methods, of which a new ML read-only recording is developed. This recording method integrates amplitude modulation achieved by the reaction mechanism of physics and chemistry of photoresist with the run-length-limited technology. The discs can be achieved using standard photoresist mastering and replication techniques with great compatibility to conventional binary read-only discs.

The 2018 M_S 5.9 Mojiang Earthquake:Source model and intensity based on near-field seismic recordings

On September 8, 2018, an M_S 5.9 earthquake struck Mojiang, a county in Yunnan Province, China. We collect near-field seismic recordings(epicentral distances less than 200 km) to relocate the mainshock and the aftershocks within the first 60 hours to determine the focal mechanism solutions of the mainshock and some of the aftershocks and to invert for the finite-fault model of the mainshock.The focal mechanism solution of the mainshock and the relocation results of the aftershocks constrain the mainshock on a nearly vertical fault plane striking northeast and dipping to the southeast. The inversion of the finite-fault model reveals only a single slip asperity on the fault plane. The major slip is distributed above the initiation point, ～14 km wide along the down-dip direction and ～14 km long along the strike direction, with a maximal slip of ～22 cm at a depth of ～6 km. The focal mechanism solutions of the aftershocks show that most of the aftershocks are of the strike-slip type, a number of them are of the normal-slip type, and only a few of them are of the thrust-slip type.On average, strike-slip is dominant on the fault plane of the mainshock, as the focal mechanism solution of the mainshock suggests, but when examined in detail, slight thrust-slip appears on the southwest of the fault plane while an obvious part of normal-slip appears on the northeast, which is consistent with what the focal mechanism solutions of the aftershocks display. The multiple types of aftershock focal mechanism solutions and the slip details of the mainshock both suggest a complex tectonic setting, stress setting, or both. The intensity contours predicted exhibit a longer axis trending from northeast to southwest and a maximal intensity of Ⅷ around the epicenter and in the northwest.

An Effective Method to Determine the Distribution Boundary of Cobalt-Rich Fe-Mn Crusts on a Guyot: Synchronous Application of Sub-bottom Profiling and Deep-Sea Video Recording

Determining the upper boundary of the cobalt-rich crust distribution of a guyot is important for estimating the mineral resources available, however, it has also long been an unsolved question. Correlations between the sub-bottom structures, revealed by sub-bottom profiling, and crust distribution can be revealed for the first time by the synchronous application of sub-bottom profiling and deep-sea video recording. The lower boundary of the sediment corresponds with the upper boundary of the crust. By analysis of these two kinds of data, the lower boundary of the sediment can be determined; therefore, the upper boundary of the crust distribution can be deduced. According to this method of analysis, the upper boundary of water depth of crust distribution of a seamount in the western Pacific is about 1 560 m.

Egg laying behavior of common cuckoos(Cuculus canorus): Data based on field video-recordings

DEAR EDITOR,The egg laying behavior of brood parasites is at the heart of studies on host co-evolution.Therefore,research on egg laying behavior can improve our understanding of brood parasitism and associated processes.Over a seven year study period,we monitored 455 oriental reed warbler(Acrocephalus orientalis)nests during the egg laying period,250 of which were parasitized by common cuckoos(Cuculus canorus).We collected 53 clear videos of common cuckoo parasitism,analyzed all recorded parasitic behavior in detail.

Scattering-Suppression in Photochromic LiNbO_(3):Fe:Mn Non-volatile Holographic Recording

We propose and experimentally investigate a new scheme capable of suppressing the light-induced scattering by periodical incoherent erasure during every non-volatile holographic recording cycle in photochromic LiNbO_(3):Fe:Mn crystals.The results demonstrate that the scattering noise is suppressed effectively,and the final diffraction efficiency of the fixed grating is significantly enhanced,rather than decrease,by about 30%compared with the conventional recording procedure.The period of the recording and incoherent erasure cycle is theoretically calculated and experimentally optimized.

In vivo patch clamp recording technique in the study of neurophysiology

The patch clamp recording technique in vivois a blind patch clamp recording methods to record the current of the spinal or cereral neurons of anaes：hesia （ or awake） animals. This technique can be used to study the synaptic function and plasticity in central nervous system in vivoin order to understand the physiological properties of the ion channels from an integrated point of view. The advantage of this technique have already presented itself in the study of the synaptic transmission and nervous network. Nowadays, in vivo patch whole-cell recording technique in combination with other techniques is becoming a common method in the research fields.

Magnetic property and microstructure of SmCo magnetic recording films

Cr/SmCo/Cr thin films with Sm concentration of 37.7 at.% were deposited on glass substrates by magnetron sputtering. Measurement of magnetic properties showed that the SmCo film possessed good magnetic anisotropy, a high coercivity of 3019 kA/m and low magnetic exchange coupling. Microstructure analysis showed that crystallized SmCo5 magnetic phase, non-magnetic SmCo2 phase and Sm2Co7 phase co-existed in the film. The non-magnetic SmCo2 phase might function as isolator of SmCo grains, leading to a decrease of magnetic exchange coupling. Moreover, a Cr2O3 oxide layer which could protect the SmCo layer from oxidation formed at the surface of the Cr cap layer.

Whole-cell recordings of calcium and potassium currents in acutely isolated smooth muscle cells

AIM： To record calcium and potassium currents in acutely isolated smooth muscle cells of mesenteric arterial branches in rats. METHODS： Smooth muscle cells were freshly isolated by collagenase digest and mechanical trituration with polished pipettes. Patch clamp technique in whole-cell mode was employed to record calcium and potassium currents. RESULTS： The procedure dissociated smooth muscle cells without impairing the electrophysiological characteristics of the cells. The voltage-gated Ca^2＋ and potassium currents were successfully recorded using whole-cell patch clamp configuration. CONCLUSION： The method dissociates smooth muscle cells from rat mesenteric arterial branches. Voltage-gated channel currents can be recorded in this preparation.

Whole-cell recording of the robust nucleus of the arcopallium neurons in the adult zebra finch

BACKGROUND： Electrophysiological properties of the song nucleus have been revealed using conventional techniques, such as intracellular and extracellular recording. Research concerning the neuronal activation properties and regulations of the song system at the cellular and ion channel level may help reveal the neural mechanism of song learning. OBJECTIVE： To perform whole-cell recording of robust nucleus of the arcopallium （RA） neurons in brain slices from adult zebra finches （Taeniopygia guttata） and observe the action potential, sodium/potassium current and the spontaneous postsynaptic current of RA neurons. DESIGN, TIME AND SETTING： Self-controlled, neuroelectrophysiological experiment. The study was performed at the Neurophysiology Laboratory of South China Normal University from April to September 2008. MATERIALS： Flaming/Brown puller P-97 was purchased from Sutter Ins, USA; Axopatch 700B amplifier and Digidata 1332A converter were purchased from Axon Instrument, USA; pClamp software was provided by Axon Instrument, USA. METHODS： RA neurons were acutely isolated from 24 healthy male zebra finches. The action potential, voltage-gate sodium/potassium current and spontaneous postsynaptic current were recorded by whole-cell recording technology. Data were analyzed by pClamp software. MAIN OUTCOME MEASURES： The amplitude and frequency of the action potential, and the amplitude of the voltage-dependent and spontaneous postsynaptic currents, were measured. RESULTS： （1） Testing of action potential： Cells exhibited a stable current-voltage relationship following a series of hyperpolarization stepped currents, and an action potential was triggered by the spike threshold. All the recorded cells displayed repetitive firing following depolarizing current injection, with a frequency beyond 100 Hz. （2） Testing of voltage-gate currents： The inward and outward whole-cell currents were observed after a series of depolarizing voltage steps. The inward current disappeared following the application of tetrodotoxin and the outward current was significantly inhibited by application of 4-aminopyfidione and tetraethylammonium chloride. （3） Testing of spontaneous postsynaptic current： The majority of recorded cells exhibited an inward synaptic current when the membrane potential was maintained at -60 mV, with some cells exhibiting a robustly outward current when the membrane potential was maintained at -30 mV. Tetrodotoxin was unable to affect the spontaneous postsynaptic current. Following application of bicuculline [y-aminobutyric acid （A） receptor antagonist] and high concentration kynurenic acid （ionotropic glutamate receptor antagonist）, the inward and outward currents were completely inhibited. CONCLUSION： Under these experimental conditions, the action potential, sodium/potassium current and spontaneous postsynaptic current were recorded successfully in RA neurons. This indicates that the cells preserved relatively intact synaptic connections and normal physiological activity, which is required for investigating ion channels. The inward and outward whole-cell currents were sodium and potassium currents, respectively. The postsynaptic y-aminobutyric acid （A） receptors and ionotropic glutamate receptors contributed to the spontaneous postsynaptic current.

High‑Performance Flexible Microneedle Array as a Low‑Impedance Surface Biopotential Dry Electrode for Wearable Electrophysiological Recording and Polysomnography

Microneedle array(MNA)electrodes are an effective solution to achieve high-quality surface biopotential recording without the coordination of conductive gel and are thus very suitable for long-term wearable applications.Existing schemes are limited by flexibility,biosafety,and manufacturing costs,which create large barriers for wider applications.Here,we present a novel flexible MNA electrode that can simultaneously achieve flexibility of the substrate to fit a curved body surface,robustness of microneedles to penetrate the skin without fracture,and a simplified process to allow mass production.The compatibility with wearable wireless systems and the short preparation time of the electrodes significantly improves the comfort and convenience of electrophysiological recording.The normalized electrode–skin contact impedance reaches 0.98 kΩcm^(2)at 1 kHz and 1.50 kΩcm^(2)at 10 Hz,a record low value compared to previous reports and approximately 1/250 of the standard electrodes.The morphology,biosafety,and electrical/mechanical properties are fully characterized,and wearable recordings with a high signal-to-noise ratio and low motion artifacts are realized.The first reported clinical study of microneedle electrodes for surface electrophysiological monitoring was conducted in tens of healthy and sleep-disordered subjects with 44 nights of recording(over 8 h per night),providing substantial evidence that the electrodes can be leveraged to substitute for clinical standard electrodes.

Improvement of Mechanical Stability for Single Unit Recording Based on Skull Cap in Living Chinchilla

Three-point head fixation was constructed to provide mechanical stability for single unit recording(SUR)on vestibular sensory system in living chinchilla previously.However,it is no more qualified to this work when the stimulation intensity becomes large because of frequent unit losing and neuron damage,which strongly implies that the mechanical stability has been broken during the stimulation.Here,we constructed a novel hea0 fixation(skull cap assistant head fixation)provided by skull cap on the basis of three-point head fixation in order to improve the mechanical stability for SUR under the stimulation with large magnitude.The large area bone connection is the feature and advantage of this improved method,which directly fixes the tested local nervous tissue and microelectrode in an intact stable system through skull cap except two ear bars and a tube face mask.Our data exhibited that skull cap assistant head fixation could significantly improve the success rate of neural response activity recording in the population of semicircular canal neurons under the stimulation with large intensity(amplitudd 00 deg/s).Based on the analysis of neural response activity and noise base-line during stimulation,our data further indicated that this method could significantly improve the mechanical stability for SUR during high-speed motion stimulation on vestibular system in living chinchilla.Skull cap assistant head fixation extends the application of SUR on vestibular neuron in linear response range and provides a solid foundation for electrophysiological research on vestibular sensory system in fhrther studies.