Objective: Ethanol treatment induces an increase in oxidative stress. As licorice compounds are potent antioxidants, our aim was to examine whether magnesium isoglycyrrhizinate attenuated lipid peroxidation, the majo...Objective: Ethanol treatment induces an increase in oxidative stress. As licorice compounds are potent antioxidants, our aim was to examine whether magnesium isoglycyrrhizinate attenuated lipid peroxidation, the major end-point of oxidative damage resulting from ethanol administration. Methods: Four groups(18 animals in each group) of male Kunming mice were used. The first group served as control and received 0.4 ml normal saline daily for 18 days orally. The second group of mice was given 56% ethanol at 16 ml/kg body weight per day for 18 days orally. The third group was given the same dose of ethanol and administrated magnesium isoglycyrrhizinate (15 mg/kg.d, i.p.) for 18 days. The fourth group was given the same dose of ethanol and administrated with magnesium isoglycyrrhizinate (45 mg/kg.d, i.p.) for 18 days. Twenty four hours after 9 days or 18 days of treatment the mice were sacrificed using 10% chloral hydrate. Sperm counts and motility in the epididymis were assessed. The lipid peroxidation and antioxidants of testicular mitochondria were also determined. The pathological changes of testicle tissue of the mice were observed by light microscopy. Results: Magnesium isoglycyrrhizinate effectively prevented the ethanol-induced seminiferous epithelium disorganization and degeneration of Sertoli cells and germ cells. Sperm counts and motility of the magnesium isoglycyrrhizinate treated groups were higher than those of the alcohol treated group, but were lower than those of the control group. The drug exhibited an ability to counteract ethanol induced oxidative challenge as it effectively reduced testicular malondialdehyde (MDA) and increased the activities of superoxide dismutase and glutathione peroxidase. Conclusion: Magnesium isoglycyrrhizinate is able to inhibit the ethanol-induced lipid peroxidation and has a protective effect against testicular oxidative injury.展开更多
Background and Aims:Oxaliplatin is widely used in can-cer chemotherapy with adverse effects such as liver toxicity.Magnesium isoglycyrrhizinate(MgIG)has hepatoprotective effects,but the underlying mechanism remains el...Background and Aims:Oxaliplatin is widely used in can-cer chemotherapy with adverse effects such as liver toxicity.Magnesium isoglycyrrhizinate(MgIG)has hepatoprotective effects,but the underlying mechanism remains elusive.The study’s aim was to investigate the mechanism underlying the hepatoprotective effects of MgIG against oxaliplatin-induced liver injury.Methods:A xenografted colorectal cancer mouse model was established with MC38 cells.Mice were given ox-aliplatin(6 mg/kg/week)for 5 weeks to mimic oxaliplatin-induced liver injury in vivo.LX-2 human hepatic stellate cell s(HSCs)were employed for in vitro studies.Serological tests,hematoxylin and eosin staining,oil red O staining and trans-mission electron microscopy were used for histopathological examinations.Real-time PCR,western blotting,immuno-fluorescence and immunohistochemical staining were used to determine Cx43 mRNA or protein levels.Flow cytometry was used to assay reactive oxygen species(ROS)and mito-chondrial membrane.Short hairpin RNA targeting Cx43 was lentivirally transduced in LX-2 cells.Ultra-high performance liquid chromatography-tandem mass spectrometry was used to determine MgIG and metabolite concentration.Results:MgIG(40 mg/kg/day)treatment significantly reduced se-rum aspartate transaminase(AST)and alanine transami-nase(ALT)levels in the mouse model,and alleviated liver pathological changes,including necrosis,sinusoidal expan-sion,mitochondrial damage,and fibrosis.MgIG reduced the abnormal expression of Cx43 in the mitochondria and nuclei of HSCs.MgIG inhibited the activation of HSCs via reducing ROS generation,mitochondrial dysfunction,and N-cadherin transcription.MgIG’s inhibition of HSCs activation was abol-ished after knockdown of Cx43 in LX-2 cells.Conclusions:Cx43 mediated MgIG’s hepatoprotective effects against ox-aliplatin-induced toxicity.展开更多
文摘Objective: Ethanol treatment induces an increase in oxidative stress. As licorice compounds are potent antioxidants, our aim was to examine whether magnesium isoglycyrrhizinate attenuated lipid peroxidation, the major end-point of oxidative damage resulting from ethanol administration. Methods: Four groups(18 animals in each group) of male Kunming mice were used. The first group served as control and received 0.4 ml normal saline daily for 18 days orally. The second group of mice was given 56% ethanol at 16 ml/kg body weight per day for 18 days orally. The third group was given the same dose of ethanol and administrated magnesium isoglycyrrhizinate (15 mg/kg.d, i.p.) for 18 days. The fourth group was given the same dose of ethanol and administrated with magnesium isoglycyrrhizinate (45 mg/kg.d, i.p.) for 18 days. Twenty four hours after 9 days or 18 days of treatment the mice were sacrificed using 10% chloral hydrate. Sperm counts and motility in the epididymis were assessed. The lipid peroxidation and antioxidants of testicular mitochondria were also determined. The pathological changes of testicle tissue of the mice were observed by light microscopy. Results: Magnesium isoglycyrrhizinate effectively prevented the ethanol-induced seminiferous epithelium disorganization and degeneration of Sertoli cells and germ cells. Sperm counts and motility of the magnesium isoglycyrrhizinate treated groups were higher than those of the alcohol treated group, but were lower than those of the control group. The drug exhibited an ability to counteract ethanol induced oxidative challenge as it effectively reduced testicular malondialdehyde (MDA) and increased the activities of superoxide dismutase and glutathione peroxidase. Conclusion: Magnesium isoglycyrrhizinate is able to inhibit the ethanol-induced lipid peroxidation and has a protective effect against testicular oxidative injury.
基金the Open Project Program of Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica(No.JKLPSE201501)the Open Project of Chinese Materia Medica First-Class Discipline of Nanjing University of Chinese Medicine(No.2020YLXK20)the Science and Technology Development Foundation of Nanjing Medical University(No.NMUB2019186).
文摘Background and Aims:Oxaliplatin is widely used in can-cer chemotherapy with adverse effects such as liver toxicity.Magnesium isoglycyrrhizinate(MgIG)has hepatoprotective effects,but the underlying mechanism remains elusive.The study’s aim was to investigate the mechanism underlying the hepatoprotective effects of MgIG against oxaliplatin-induced liver injury.Methods:A xenografted colorectal cancer mouse model was established with MC38 cells.Mice were given ox-aliplatin(6 mg/kg/week)for 5 weeks to mimic oxaliplatin-induced liver injury in vivo.LX-2 human hepatic stellate cell s(HSCs)were employed for in vitro studies.Serological tests,hematoxylin and eosin staining,oil red O staining and trans-mission electron microscopy were used for histopathological examinations.Real-time PCR,western blotting,immuno-fluorescence and immunohistochemical staining were used to determine Cx43 mRNA or protein levels.Flow cytometry was used to assay reactive oxygen species(ROS)and mito-chondrial membrane.Short hairpin RNA targeting Cx43 was lentivirally transduced in LX-2 cells.Ultra-high performance liquid chromatography-tandem mass spectrometry was used to determine MgIG and metabolite concentration.Results:MgIG(40 mg/kg/day)treatment significantly reduced se-rum aspartate transaminase(AST)and alanine transami-nase(ALT)levels in the mouse model,and alleviated liver pathological changes,including necrosis,sinusoidal expan-sion,mitochondrial damage,and fibrosis.MgIG reduced the abnormal expression of Cx43 in the mitochondria and nuclei of HSCs.MgIG inhibited the activation of HSCs via reducing ROS generation,mitochondrial dysfunction,and N-cadherin transcription.MgIG’s inhibition of HSCs activation was abol-ished after knockdown of Cx43 in LX-2 cells.Conclusions:Cx43 mediated MgIG’s hepatoprotective effects against ox-aliplatin-induced toxicity.